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Ci X.-K.,Shandong Agricultural University | Ci X.-K.,Chinese Academy of Agricultural Sciences | Liu H.-L.,Shandong Agricultural University | Liu H.-L.,Circulation Industry Promotion Center | And 4 more authors.
Journal of Integrative Agriculture | Year: 2012

A pot experiment was conducted to investigate the effect of different arsenic (As) levels on maize (Zea mays L.) growth and As accumulation and species in different parts of maize plants, as a guideline for production of maize in As-polluted areas with the objective of preventing As from entering the food chain, and improving understanding of the mechanisms of effect of As on plant. Zhengdan 958 was grown at five As levels added to soil (0, 12.5, 25, 50, and 100 mg kg-1 As). As concentration in maize tissues increased in the order of grain Source

Hao Y.-B.,Shandong Agricultural University | Hao Y.-B.,Heilongjiang Academy of Agricultural science | Liu H.-L.,Circulation Industry Promotion Center | Ci X.-K.,Shandong Agricultural University | And 5 more authors.
Chinese Journal of Applied Ecology | Year: 2012

A pot experiment with conventional maize cultivar ZD958 and glutinous maize cultivar JN218 was conducted to study the effects of applying different concentrations(0, 10, 25 and 50 mg·kg-1) of selenium(Se) on the Se allocation in plant organs, grain yield, and its quality. At low concentrations(≤10 mg·kg-1), Se stimulated maize growth, and increased biomass accumulation and grain yield significantly. At high concentrations(≥25 mg·kg-1), Se inhibited maize growth, and decreased dry mass accumulation, grain yield, and its quality. The Se concentration in plant organs was in the order of root > leaf > stalk > sheath. The Se concentrations in plant organs had a positive correlation with the Se concentration in soil. Comparing with ZD958, JN218 could accumulate more Se in natural low-Se environment, but enrich lesser Se in the environment with 10 mg·kg-1 of Se. Taking the Se accumulation amount in grain and aboveground vegetative organs as the standard for evaluation, JN218 was more available planted on natural low-Se(0.25 mg·kg-1) soil or high-Se(25 mg·kg-1) soil, while ZD958 was appropriate planted on Se-rich(10 mg· kg-1) soil or Se-polluted(50 mg·kg-1) soil. Source

JIN W.-J.,Yangzhou University | ZHENG Z.-M.,Yangzhou University | ZHENG Z.-M.,Circulation Industry Promotion Center | WANG Q.-Q.,Yangzhou University | And 3 more authors.
Journal of Integrative Agriculture | Year: 2012

Fosfomycin, a broad-spectrum antibiotic against both Gram-positive and Gram-negative bacteria, is very important in the clinic but many fosfomycin-resistant bacteria have been isolated from patients. In this study, the resistance mechanism of three fosfomycin-resistant avian pathogenic Escherichia coli (APEC) strains (JE1, IF7 and CD11) isolated from septicemic chickens were analyzed. The results showed that their fosfomycin-resistance mechanisms were different. An alteration in the glpT transport system was the main reason of the fosfomycin-resistance mechanisms of strain IF7. Compared with the control stain BL21, the capacity of fosfomycin-uptake was low in all these three stains (JE1>IF7>CD11). Sequence results of murA showed that there were more than 10 sites of nucleotide mutation, but only one amino acid mutation T116A showed in CD11. Real-time detection test showed that the expression level of the murA gene of the three stains was significantly increased (four times increase in strain CD11 and two times increase in strains JE1 and IF7). The transformation and recombinant test showed that the recombinant bacteria with the murA of JE1 and CD11 showed high minimal inhibitory concentration (MIC) against fosfomycin. From the results of this research, it showed that most of the fosfomycin-resistance mechanisms once showed in patient bacteria have appeared in the APEC strains and the fosfomycin-resistance mechanism of the three APEC isolates was different. © 2012 Chinese Academy of Agricultural Sciences. Source

Hou X.,China Agricultural University | Li Y.,Circulation Industry Promotion Center | Zhang Z.,China Agricultural University | Wu Y.,Institute of Nutrition and Food Hygiene
Chromatographia | Year: 2010

Matrix solid-phase dispersion (MSPD) with alumina N as adsorbent has been used for extraction of para red, Sudan 1, Sudan 2, Sudan 3, and Sudan 4 dyes from egg yolk. The extracts were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). Mean recovery for the five dyes ranged from 63.2 to 98.6%, with CV 0.55-10.00%. One sample was confirmed to contain 0.3 mg kg-1 Sudan 4. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH. Source

Guo W.,Wuhan University | Guo W.,Circulation Industry Promotion Center | Hu S.,Wuhan University | Li X.,Circulation Industry Promotion Center | And 4 more authors.
Talanta | Year: 2011

Direct determination of trace arsenic in high chlorine food samples by ICP-MS is complicated by the presence of ArCl+ interferences, and the high first ionization energy of As (9.81 eV) also results in low analytical sensitivity in ICP-MS. In this work, two strategies based on ion-molecule reactions were successfully used to eliminate ArCl spectral interference in a dynamic reaction cell (DRC). The interference ion (40Ar 35Cl+) was directly removed by the reaction with methane gas, and the background signal was reduced by up to 100-fold at m/z 75. Alternatively, by using molecule oxygen as the reaction gas, 75As+ was effectively converted to 75As16O+ that could be detected at m/z 91 where the background is low. The poor signal intensity of As or AsO was improved 3-4 times by addition of 4% methanol in the analyzed solutions. The limit of quantitation (LOQ) for 75As (CH 4-DRC method) and 75As16O (O2-DRC method) was 0.8 and 0.3 ng g?1 and the analytical results of seaweed and yellow croaker standard reference materials were in good agreement with the certified values. As the routine arsenic monitoring method in our laboratory, it was applied to the accuracy determination of 119 high chlorine food samples from eight different markets of Beijing. © 2011 Elsevier B.V. All rights reserved. Source

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