Veterans Affairs Medical Center Cincinnati

Cincinnati, OH, United States

Veterans Affairs Medical Center Cincinnati

Cincinnati, OH, United States

Time filter

Source Type

Patel Z.H.,Cincinnati Childrens Hospital Medical Center | Patel Z.H.,University of Cincinnati | Kottyan L.C.,Cincinnati Childrens Hospital Medical Center | Kottyan L.C.,Veterans Affairs Medical Center Cincinnati | And 17 more authors.
Frontiers in Genetics | Year: 2014

Next Generation Sequencing studies generate a large quantity of genetic data in a relatively cost and time efficient manner and provide an unprecedented opportunity to identify candidate causative variants that lead to disease phenotypes. A challenge to these studies is the generation of sequencing artifacts by current technologies. To identify and characterize the properties that distinguish false positive variants from true variants, we sequenced a child and both parents (one trio) using DNA isolated from three sources (blood, buccal cells, and saliva). The trio strategy allowed us to identify variants in the proband that could not have been inherited from the parents (Mendelian errors) and would most likely indicate sequencing artifacts. Quality control measurements were examined and three measurements were found to identify the greatest number of Mendelian errors. These included read depth, genotype quality score, and alternate allele ratio. Filtering the variants on these measurements removed ~95% of the Mendelian errors while retaining 80% of the called variants. These filters were applied independently. After filtering, the concordance between identical samples isolated from different sources was 99.99% as compared to 87% before filtering. This high concordance suggests that different sources of DNA can be used in trio studies without affecting the ability to identify causative polymorphisms. To facilitate analysis of next generation sequencing data, we developed the Cincinnati Analytical Suite for Sequencing Informatics (CASSI) to store sequencing files, metadata (eg. relatedness information), file versioning, data filtering, variant annotation, and identify candidate causative polymorphisms that follow either de novo, rare recessive homozygous or compound heterozygous inheritance models. We conclude the data cleaning process improves the signal to noise ratio in terms of variants and facilitates the identification of candidate disease causative polymorphisms. © 2014 Patel, Kottyan, Lazaro, Williams, Ledbetter, Tromp, Rupert, Kohram, Wagner, Husami, Qian, Valencia, Zhang, Hostetter, Harley and Kaufman.

Loading Veterans Affairs Medical Center Cincinnati collaborators
Loading Veterans Affairs Medical Center Cincinnati collaborators