CIIDIR IPN

Victoria de Durango, Mexico

CIIDIR IPN

Victoria de Durango, Mexico

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Atighi M.R.,University of Tehran | Pourjam E.,University of Tehran | Pereira T.J.,University of California at Riverside | Pereira T.J.,CAPES Foundation | And 4 more authors.
Nematology | Year: 2013

Filenchus annulatus is redescribed and males are characterised for the first time based on a population found in Northern Khorasan province, Iran. Newmorphological characterisation is based on light and scanning electron microscopy. In addition, molecular analyses based on 18S and 28S genes are included to test monophyly of the genus. Females from the Iranian population have a spermatheca typically filled with sperm. Generally males are similar to females, ranging from 306 to 426 μm long. Spicules are arcuate, cephalated and 11.5-14.0 μm long, the gubernaculum is minute and trough-shaped and the caudal alae are adanal. Phylogenetic analyses differed in results depending on the gene used: 28S gene strongly supports Filenchus as monophyletic whereas 18S shows Filenchus as polyphyletic. In both gene phylogenies, F. annulatus is placed as a sister taxon of F. quartus from Wyoming, USA. Although sequence divergence between these two species is only 3 base pairs and 1 base pair for 28S and 18S genes, respectively, strong morphological differences support their species status. Relationships between Filenchus and other Tylenchidae genera are also gene dependent. Such differences in tree topologies and branch support are related to the number of Filenchus species used in the analyses (greater for 18S gene) and gene resolution (greater for 28S gene). Molecular phylogenies also suggest that other Tylenchidae genera (i.e., Psilenchus, Cephalenchus and Eutylenchus) belong to separate clades, as is also suggested by some morphology-based classifications. The inclusion of more taxa and perhaps additional genes is needed further to clarify Filenchus relationships and further to test its monophyly. Copyright © Koninklijke Brill NV, Leiden, 2013.


Reyes Estrada O.,Universidad Juárez del Estado de Durango | Murillo Ortiz M.,Universidad Juárez del Estado de Durango | Herrera Torres E.,Universidad Juárez del Estado de Durango | Gurrola Reyes J.,CIIDIR IPN | Juarez Reyes A.,Universidad Juárez del Estado de Durango
Journal of Animal and Veterinary Advances | Year: 2010

We used four ruminally cannulated steers (350±3 kg) to evaluate the effect of season on chemical composition, intake, kinetic, digestibility and ruminal fermentation of diet selected during grazing. Data were analyzed as a randomized complete block design with months as the block and season of year as treatment. Time-sequence data than pH, ammonia Nitrogen (NH3N) and Volatile Fatty Acids (VFA) were analyzed as repeated measures within a randomized block design. The Dry Matter Intake (DMI), Organic Matter Intake (OMI), Digestible Energy (DE), Metabolizable Energy (ME) and passage rate (Kp) of diet were affected by season (p<0.05). The Crude Protein (CP) and NH3N concentration were higher in the rain season (p<0.01). The content of NDF was different in seasons (p<0.01). Similar, acetate, propionate and butyrate concentrations were different (p<0.01). Changes in chemical composition, intake, kinetic, digestibility and ruminal fermentation observed in this study appeared to be related to season. © Medwell Journals, 2010.


Gallegos-Infante J.A.,Durango Institute of Technology | Rocha-Guzman N.E.,Durango Institute of Technology | Gonzalez-Laredo R.F.,Durango Institute of Technology | Medina-Torres L.,National Autonomous University of Mexico | And 5 more authors.
Food Bioscience | Year: 2013

The effect of two wall materials and two feed flow rates on the physical and antioxidant properties of Quercus resinosa leaf infusion microencapsulated by spray-drying is reported. Dispersions with lyophilized Q. resinosa infusion and wall material (k-carrageenan and maltodextrin [10 DE]) were prepared. Samples were fed at rate flows of 1.6 and 1.7 L/h. Encapsulation yield, total phenolic content, DPPH test, deoxy-D-ribose assayt, rheological and SEM evaluations were made. The highest yield and DPPH scavenging activity were obtained at 100% maltodextrin and 1.7 L/h. Higher polyphenolic retention was observed in blends of carrageenan/maltodextrin (1.6 and 1.7 L/h) and maltodextrin (1.6 L/h). The highest inhibition of deoxy-D-ribose oxidation was found at blends of carrageenan/maltodextrin (1.7 L/h). Dispersions showed pseudoplastic behavior and properties as liquid-like materials. Microcapsules showed particle sizes between 5 and 35 |im. The best condition for encapsulation of Q. resinosa infusions was suggested as 100% maltodextrin at 1.7 L/h. © 2012 Elsevier Ltd.


Troconis-Torres I.G.,National Polytechnic Institute of Mexico | Rojas-Lopez M.,Research Center en Biotecnologia Aplicada | Hernandez-Rodriguez C.,National Polytechnic Institute of Mexico | Villa-Tanaca L.,National Polytechnic Institute of Mexico | And 4 more authors.
Journal of Biomedicine and Biotechnology | Year: 2012

The genus Capsicum provides antioxidant compounds, such as phenolics and carotenoids, into the diet. In Mexico, there is a wide diversity of species and varieties of chilli peppers, a fruit which has local cultural and gastronomic importance. In the present study, the relationship of the carotenoid and phenolic profiles with the RAPD fingerprint of three different commercial cultivars of chilli peppers of seven regions of Mexico was investigated. Through RAPD, the species of chilli were differentiated by means of different primers (OPE-18, MFG-17, MFG-18, C51, and C52). The genetic distance found with OPE 18 was in the order of 2.6. The observed differences were maintained when the chromatographic profile of carotenoids, and the molecular markers were analyzed, which suggest a close relationship between carotenoids and the genetic profile. While the chromatographic profile of phenols and the molecular markers were unable to differentiate between genotypes of chilli peppers. In addition, by using infrared spectroscopy and statistical PCA, differences explained by geographic origin were found. Thus, this method could be an alternative for identification of chilli species with respect to their geographic origin. Copyright © 2012 Ivonne Guadalupe Troconis-Torres et al.


Campos-Enriquez J.O.,National Autonomous University of Mexico | Corbo-Camargo F.,National Autonomous University of Mexico | Arzate-Flores J.,National Autonomous University of Mexico | Keppie J.D.,National Autonomous University of Mexico | And 3 more authors.
Journal of South American Earth Sciences | Year: 2013

Thirty magnetotelluric soundings were made along two NW-SE profiles to the north and south of Oaxaca City in southern Mexico. The profiles crossed the N-S Oaxaca Fault and the Oaxaca-Juarez terrane boundary defined by the Juarez mylonitic complex. Dimensionality analysis of the MT data showed that the subsurface resistivity structure is 2D or 3D. The Oaxaca and correlative Guichicovi terranes consist of ca. 1-1.4 Ga granulitic continental crust overlain by Phanerozoic sedimentary rocks, characterized by high and low resistivities, respectively. The Juarez terrane consists of oceanic Mesozoic metavolcanic and metasedimentary rocks, characterized by a low to medium resistivity layer, that is approximately 10 km thick. The Oaxaca Fault is a Cenozoic aged, normal fault that reactivated the dextral and thrust Juarez mylonitic complex north of Oaxaca City: its location south of Oaxaca City is uncertain. In the southern profile, the MT data show a ca. 20-50 km wide, west-dipping, relatively low resistivity zone material that extends through the entire crust. This is inferred to be the Juarez terrane bounded on either side by the ca. 1-1.4 Ga granulites. The Oaxaca Fault is imaged only by a major electrical resistivity discontinuity (low to the west, high to the east) along both the western border of the Juarez mylonitic complex (northern profile) and the San Miguel de la Cal mountains (southern profile) suggesting continuity. © 2013 Elsevier Ltd.


Abebe E.,Elizabeth City State University | Sharma J.,University of Texas at San Antonio | Mundo-Ocampo M.,CIIDIR IPN | Platt T.R.,Saint Mary's College
Nematology | Year: 2012

Examination of perianal folds of stinkpot turtles, Sternotherus odoratus, from Virginia, USA, revealed several monhysterid nematodes. The general morphological characteristics of this population, such as circular amphids, unstriated cuticle under light microscope, single outstretched ovary, single testis, caudal glands, terminal spinneret and the absence of an apophysis on the gubernaculum, with the exception of the position of the gonad in relation to the intestine, shows the affinity of this population to the Monhysteridae. Microscopic observations of the inner stoma show three (one dorsal and two ventro-sublateral) buccal wall extensions towards the lumen and numerous (ca 50) distinct denticles on its wall. Because this stoma structure is a morphological novelty within the Monhysterida and this is the first monhysterid group associated with perianal folds of turtles, we propose to establish Testudinema n. gen. within the Monhysteridae to accommodate this morphologically distinct population. We provide a detailed comparison with known monhysterid genera including those that possess a cup-shaped stoma, stoma with denticles or those genera reported to be commensals (Gammarinema, Monhystrium, Tripylium, Odontobius). Stinkpot turtles feed on benthic fauna and may acquire these commensals when the sediment is disturbed. The denticles and structure of the buccal cavity could be morphological adaptations for transition to ecto-parasitism. © Koninklijke Brill NV., Leiden, 2012.


PubMed | CIIDIR IPN and Autonomous University of Sinaloa
Type: Journal Article | Journal: World journal of microbiology & biotechnology | Year: 2016

Maize is an economically important crop in northern Mexico. Different fungi cause ear and root rot in maize, including Fusarium verticillioides (Sacc.) Nirenberg. Crop management of this pathogen with chemical fungicides has been difficult. By contrast, the recent use of novel biocontrol strategies, such as seed bacterization with Bacillus cereus sensu lato strain B25, has been effective in field trials. These approaches are not without their problems, since insufficient formulation technology, between other factors, can limit success of biocontrol agents. In response to these drawbacks, we have developed a powder formulation based on Bacillus B25 spores and evaluated some of its characteristics, including shelf life and efficacy against F. verticillioides, in vitro and in maize plants. A talc-based powder formulation containing 1 10(9) c.f.u. g(-1) was obtained and evaluated for seed adherence ability, seed germination effect, shelf life and antagonism against F. verticillioides in in vitro and in planta assays. Seed adherence of viable bacterial spores ranged from 1.0 to 1.41 10(7) c.f.u. g(-1). Bacteria did not display negative effects on seed germination. Spore viability for the powder formulation slowly decreased over time, and was 53 % after 360 days of storage at room temperature. This formulation was capable of controlling F. verticillioides in greenhouse assays, as well as eight other maize phytopathogenic fungi in vitro. The results suggest that a talc-based powder formulation of Bacillus B25 spores may be sufficient to produce inoculum for biocontrol of maize ear and root rots caused by F. verticillioides.


Murillo M.,Mexico State University | Herrera E.,Mexico State University | Reyes O.,Mexico State University | Gurrola J.N.,CIIDIR IPN | Gutierrez E.,Autonomous University of Nuevo León
African Journal of Agricultural Research | Year: 2011

This study evaluated the seasonal changes in the in vitro gas production of diets consumed for grazing steers. The gas produced by the soluble fraction "a" and insoluble but slowly fermenting fraction "b", were highest in summer and fall (p<0.01). The constant gas production rate "c" was affected by season of the year (p<0.01). The organic matter digestibility (OMD) and short chain fatty acids (SCFA) was affected by season of the year (p<0.05). The in vitro gas production is a good indicator of the nutritive quality diet consumed by grazing cattle. © 2011 Academic Journals.


Santos-Cervantes M.E.,CIIDIR IPN | Chavez-Medina J.A.,CIIDIR IPN | Acosta-Pardini J.,Sonora Institute of Technology | Flores-Zamora G.L.,CIIDIR IPN | And 2 more authors.
Plant Disease | Year: 2010

Potato purple top (PPT) disease has caused severe economic losses in some potato (Solanum tuberosum) growing areas of Mexico. Two distinct phytoplasma strains belonging to the aster yellows and peanut witches'-broom groups (16SrI and 16SrII groups) have been associated with PPT disease in several regions of Mexico. However, there has been no previous large-scale survey in the main potato growing areas in Mexico to analyze the diversity and geographical distribution of phytoplasmas. Potato samples were collected between 2003 and 2006 and were analyzed by nested polymerase chain reaction assays. On the basis of results from nucleotide sequence comparisons and virtual restriction fragment length polymorphism analysis of 16S rDNA, four different phytoplasma groups were detected in potato growing areas in Mexico. The aster yellows group (16SrI) 'Candidatus Phytoplasma asteris' was distributed in all potato growing areas, whereas peanut witches'-broom group (16SrII) 'Candidatus Phytoplasma aurantifolia' was detected in Guanajuato and Sinaloa, X-disease group (16SrIII) was detected in Coahuila and Guanajuato, and the Mexican periwinkle virescence (16SrXIII) was only detected in Sinaloa. Phytoplasmas from X-disease and Mexican periwinkle virescence groups were detected in potato samples for the first time in Mexico. © 2010 The American Phytopathological Society.


The quantitative importance of rainfall interception loss and the performance of the reformulated Gash model were evaluated as a function of basal area in Mexico's northeastern temperate forest communities. A sensitivity analysis as well as an iterative search of parameters matched interception loss measurements and assessments and isolated coefficient values that drive the model performance. Set hypothesis was tested with a total of 73 rainfalls recorded on four forest stands with different canopy cover for model fitting (39) and validation (34). The reformulated Gash model predicted well rainfall interception loss because mean deviations between recorded and modelled interception loss as a function of gross rainfall, MD, were <2.6% and 5.3% for fitting and validating parameter data sets, respectively. Basal area was negatively related to the model performance, but maximum projected MD range values can be found in most interception loss studies, for example, <7% when basal area is <5m2ha-1. The wet canopy evaporation rate and the canopy storage coefficient drive interception loss and the iterative parameter search showed that high wet canopy evaporation rates were expected in these forests. These parameters must be further studied to physically explain drivers of high wet canopy evaporation rates. Copyright © 2012 John Wiley & Sons, Ltd.

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