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Mérida, Mexico

Castano E.,CICY | Philimonenko V.V.,Academy of Sciences of the Czech Republic | Kahle M.,Academy of Sciences of the Czech Republic | Fukalova J.,Academy of Sciences of the Czech Republic | And 5 more authors.
Histochemistry and Cell Biology | Year: 2010

Actin is a well-known protein that has shown a myriad of activities in the cytoplasm. However, recent findings of actin involvement in nuclear processes are overwhelming. Actin complexes in the nucleus range from very dynamic chromatin-remodeling complexes to structural elements of the matrix with single partners known as actin-binding proteins (ABPs). This review summarizes the recent findings of actin-containing complexes in the nucleus. Particular attention is given to key processes like chromatin remodeling, transcription, DNA replication, nucleocytoplasmic transport and to actin roles in nuclear architecture. Understanding the mechanisms involving ABPs will definitely lead us to the principles of the regulation of gene expression performed via concerting nuclear and cytoplasmic processes. © Springer-Verlag 2010. Source

Rosas-Durazo A.,CIAD Guaymas | Lizardi J.,CIAD Hermosillo | Higuera-Ciapara I.,CICY | Arguelles-Monal W.,CIAD Guaymas | And 2 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2011

The aim of this work was to develop and characterize a new type of nanocapsules. To this end, a nanoemulsion bearing an oily core (Miglyol 812) was obtained by spontaneous emulsification and stabilized by dodecyl-trimethylammonium chloride (DTAC), a commercial cationic surfactant; this nanoemulsion was coated with proportionally very small amounts of κ-carrageenan (at molar charge ratios of Z≤ 0.0045) that interact predominantly by an electrostatic mechanism with the positively charged sites at the polar heads of DTAC at the nanoemulsion's surface to harness nanocapsules of average size ∼250-330. nm and zeta potential (ζ) ranging from ∼+80 to +7. mV. The potential application of the new type of developed nanosystems as drug delivery vehicles has yet to be investigated and fully realized. © 2011 Elsevier B.V. Source

Rosas-Durazo A.,CIAD Guaymas | Hernandez J.,University of Veracruz | Lizardi J.,CIAD Hermosillo | Higuera-Ciapara I.,CICY | And 2 more authors.
Soft Matter | Year: 2011

The gelation processes taking place in the non-stoichiometric complex between κ-carrageenan and dodecyltrimethylammonium chloride in KCl were studied. The mechanical properties of the hydrogels show a gradual reinforcement when the amount of surfactant in the complex increases, indicative of a more densely cross-linked polymer network. The thermal and viscoelastic behavior of complexes during cooling is similar to that of κ-carrageenan alone. Nevertheless, the melting of the gel splits into two endothermic processes. The emergence of a new transition during heating indicates that the overall polymer network is related to two types of networks: one formed by typical bonds between κ-carrageenan macromolecular chains and the other associated to hydrophobic blocks developing as a consequence of the cooperative nature of polyelectrolyte-surfactant interactions. In the hydrogel these two networks co-exist, probably semi-interpenetrated, somehow interconnected at the molecular level. © 2011 The Royal Society of Chemistry. Source

Ritter E.,Neiker | Herran A.,Neiker | Valdes-Infante J.,IIFT | Rodriguez-Medina N.N.,IIFT | And 14 more authors.
Acta Horticulturae | Year: 2010

Integrated parental linkage maps have been constructed in three guava mapping populations ('Enana' × "N", 'Enana' × 'Suprema Roja' and 'Enana' × 'Belic L-207') based on AFLP and SSR markers. Between 102 and 119 AFLP primer combinations (PCs) were analysed in each population, generating between 684 and 1163 segregating AFLP fragments. The distribution of parent-specific and common markers indicated that 'Enana' is less heterozygous than the other parents and that all parents share a considerable gene pool. In addition, between 28 and 171 SSR PCs were analysed for linkage mapping in these populations. Initially parent specific linked fragments were arranged into linkage groups. In all mapping population, 11 linkage groups (LGs) corresponding to the 11 chromosomes of the haploid guava genome were obtained for each parent. Based on the available SSR markers, combined parental linkage maps of each mapping population were produced using as anchor points allelic SSR fragments and common AFLP fragments. These integrated maps contain between 408 and 850 markers and have lengths of 1885 to 2179 cM, respectively. Average linkage group lengths in these maps vary between 160 and 198 cM and contain on average between 37 and 77 markers. Several identical SSR markers were mapped in various progenies, and potential associations of linkage groups from different populations were detected. In the future, the number of common SSR markers has to be increased in order to achieve full alignment of all individual linkage maps into a high-density reference molecular linkage map of guava. Source

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