National Institute Of Endocrinology Ci Parhon

Bucharest, Romania

National Institute Of Endocrinology Ci Parhon

Bucharest, Romania
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Neamtu C.,National Institute Of Endocrinology Ci Parhon | Tupea C.,National Institute Of Endocrinology Ci Parhon | Paun D.,National Institute Of Endocrinology Ci Parhon | Hoisescu A.,National Institute Of Endocrinology Ci Parhon | And 3 more authors.
Hormones | Year: 2016

Thyroid hormones (TH) exert their actions by binding nuclear receptors alpha (TRα1) and beta (TRß1 and TRß2). Resistance to thyroid hormone (RTH) is a clinical syndrome with various clinical manifestations, its hallmark being decreased tissue sensitivity to the action of thyroid hormones. We report the case of a family harbouring a novel TRß mutation. Sequencing of the TRβ gene revealed a single nucleotide substitution-C to G in codon 340: glutamine was replaced by glutamic acid. The clinical picture and biochemical and hormonal panel showed significant differences within the family, despite their sharing the same mutation. We also present the result of low-dose antithyroid treatment in one member of the family diagnosed with this rare condition. © 2016, Hellenic Endocrine Society. All rights reserved.

Albu S.E.,Carol Davila University of Medicine and Pharmacy | Carsote M.,Carol Davila University of Medicine and Pharmacy | Terzea D.,National Institute Of Endocrinology Ci Parhon | Ghemigian A.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Archives of the Balkan Medical Union | Year: 2016

We aim at introducing a series of cases regarding autoimmune thyroid conditions in adult non-pregnant women with a particular pattern: hypothyroidism and thyrotoxicosis in the same patient at different moments. A 61-year old non-smoking woman was diagnosed at age of 58 with Basedow's disease and treated for 3 months with thiamazole. She presented asymmetrical eye involvement and negative TRAB (TSH Receptor Antibodies). TSH (Thyroid Stimulating Hormone) rapidly increased under therapy but further on spontaneously when the drug was stopped thus levothyroxine was necessary. This aspect was unchanged for 3 more years. The second case is a 42-year old woman with autoimmune thyroiditis with inaugural episode of flare-up at age of 37 and remission within 6 months. Spontaneous euthyroidism continued for two years until thyrotoxicosis relapsed. High Iodine 131 uptake and elevated TRAB confirmed Basedow's disease.Thiamazole was started and controlled thyroid function. Radioiodine therapy was offered to the patient (15 mCi). Two months later, severe hypothyroidism required substitution therapy. A 65-year old woman had a two decades history of chronic thyroiditis with treated hypothyroidism. 12 years ago she was diagnosed with hepatitis C and treated with interferon, without remission. She continued thyroid substitution until recently when daily oral dose of levothyroxine was no longer necessary due to suppressed TSH. The patient refused any medication for thyroid during 2 years. Currently, primary hyperthyroidism is confirmed by thyroid scintigram, thus a short course of thyroid blocker drugs as propylthiouracil was started, to introduce a dose of radioiodine therapy. Multiple levels of interactions are found in patients with positive reaction for thyroid antibodies or chronic hepatic viral infections requiring a long-term follow-up and continuing adjustment of therapeutically approach. © 2016 CELSIUS.

Gruia A.,Medlife Clinic | Dumitru A.,National Institute Of Endocrinology Ci Parhon | Carsote M.,Carol Davila University of Medicine and Pharmacy | Morar A.,Clinical Hospital of Infectious Diseases | Valea A.,University of Medicine and Pharmacy, Cluj-Napoca
Archives of the Balkan Medical Union | Year: 2016

Vanishing testes syndrome (or bilateral anorchia), as part of 46, XY differences of sex development, may be early detected during life or partial gonadal dysgenesis are discovered during adult years with mild forms of hypogonadism. Testicular regression may have a genetic background, as mutations of steroidogenic factor 1 (SF 1), but it may be potentially related to other syndromes and anomalies as mental retard, renal anomalies, etc. We aim to introduce a complex male case with a long medical history, including late diagnosis of vanishing testes syndrome. This case introduces the challenges of distinguishing between testicular regression syndrome and ectopic testes on an adult male with mild form of hypogonadism. Particularly, the vanishing testes syndrome was associated with a compressive form of chronic thyroiditis of fibrous type, with aggressive elevation of anti-thyroid antibodies including after thyroidectomy, a large apparently benign unilateral multi-cystic mass at kidney level. Abbreviations: ATG = Anti-thyroglobulin antibodies, cm = centimetre, CT = computed tomography, TPO = Antithyreoperoxidase antibodies, TSH = Thyroid Stimulating Hormone. © 2016 CELSIUS.

Nicolae I.,Dermatovenerological Center | Schipor S.,National Institute Of Endocrinology Ci Parhon
Journal of the European Academy of Dermatology and Venereology | Year: 2010

Background Recently, it became more evident that skin is a target for neuroendocrine signals. Aims (1) To evaluate the relationship between tumour aggressiveness and hypercalcaemia in patients with non-melanoma skin cancer; (2) to identify clinical, functional, biological alterations caused by this setting; (3) calcium redistribution from extracellular fluids to intracellular compartments; (4) to describe several molecular aspects of hypercalcaemia development. Materials and methods This study was conducted between January 2000 and May 2009 in Dermatoveneorological Center, Bucharest. From the 1232 cases that were investigated, there were 32 patients with keratoachantoma, 468 patients with basal cell carcinoma, 412 patients with squamous cell carcinoma and 320 healthy volunteers. All the patients were screened by clinical and paraclinical examinations (haematology, biochemistry, immunology). After biochemical confirmation of hypercalcaemia, patients had endocrine tests, electrocardiography and imagistic approaches. Total serum calcium was measured in extracellular fluids (serum, urine) by spectrophotometric methods. Ionized calcium was calculated depending on total serum calcium and total proteins. Corrected serum total calcium (cTCa) levels were calculated using albumin and total serum calcium levels. In tumour tissues and intact skin, calcium was assayed by physical methods of analysis: Instrumental Neutron Activation Analysis (INAA), Proton-Induced X-ray Emission (PIXE). Intact PTH was measured by ELISA. Results PTH-independent hypercalcaemia prevalence is low in SCC patients (1.21%). Hypercalcaemia manifestations are multiple including: digestive, renal, neuromuscular, and cardiovascular abnormalities. In these patients, intact PTH (iPTH) is normal, urinary calcium is decreased, serum albumin is reduced, and calcium concentration in tumour tissue is significantly increased compared to healthy tissue. Conclusions PTH-independent hypercalcaemia has a low prevalence in SCC patients. Hypercalcaemia is correlated with susceptibility to develop metastases in SCC. A possible mechanism is PTHrp hypersecretion by malignant keratinocytes. © 2009 European Academy of Dermatology and Venereology.

Georgescu M.,Carol Davila University of Medicine and Pharmacy | Tampa M.,Carol Davila University of Medicine and Pharmacy | Matei C.,Carol Davila University of Medicine and Pharmacy | Paun D.L.,National Institute Of Endocrinology Ci Parhon | Year: 2014

Important changes take place during pregnancy. Electrolyte imbalance and hormone fluctuations are recognised as triggers for neurological impairment, also commonly found in otorhinolaryngology. After delivery most of these deficits spontaneously remit. Still, due to ethical considerations treatment is mandatory in order to facilitate recovery of the neurological deficit. This case report presents a 33-year pregnant woman suffering from sudden unilateral sensorineural hearing loss and tinnitus in the third trimester. Although Dextrane 40 infusion treatment was successful, with measurable improvement on the pure tone audiogram, pharmacological treatment during pregnancy has to be carefully and individually coordinated. An interdisciplinary approach in diagnostic and therapeutic measures together with gynaecologists appears obligatory.

Manolescu B.N.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,University of Bucharest | Dumitru L.,Carol Davila University of Medicine and Pharmacy | And 9 more authors.
Inflammation | Year: 2011

Stroke is a pathological condition associated with an enhanced inflammatory response that has a multifactorial etiology. We evaluated the dynamic of plasma concentrations of IL-1α, IL-6, IL-8, TNF-α, soluble form of intercellular adhesion molecule 1, and lipoprotein (a) [Lp(a)] during the rehabilitation of post-acute stroke patients (n=20), in parallel with control subjects (n = 24). Stroke patients had significantly increased concentrations of IL-6, TNF-α, and Lp(a) when compared to healthy controls. It was found that the changes in the IL-6, IL-8, and TNF-α concentrations associated with the pathological condition were statistically significant (χ 2=4.81, p=0.028, χ 2=10.40, p=0.005 and χ 2=6.73, p=0.034, respectively). The decrease of Lp(a) during the rehabilitation had statistical significance (p = 0.043), while the decrease of IL-1α had marginal significance (p = 0.071). IL-1α, TNF-α, and Lp(a) concentrations were significantly negatively correlated with the Barthel index values, suggesting that the decrease of these inflammatory markers was beneficial for patients' recovery. © 2010 Springer Science+Business Media, LLC.

Moanta M.L.,Emergency Clinical County Hospital Craiova | Dinu I.-R.,Emergency Clinical County Hospital Craiova | Mota M.,University of Medicine and Pharmacy of Craiova | Copaescu C.,Delta Hospital Bucharest National Institute of Endocrinology CI Parhon | Dumitrache C.,National Institute of Endocrinology CI Parhon
Romanian Journal of Diabetes, Nutrition and Metabolic Diseases | Year: 2011

Obesity has become a serious health problem and it is a major risk factor for the development of insulin resistance and type 2 diabetes mellitus (T2DM). Laparoscopic sleeve gastrectomy (LSG) has revealed to be effective for achieving weight loss and apotential competitor with other operations. The aim of this study is to evaluate weight loss and the reversibility of associated comorbidities in obese T2DMpatients, one and three months after LSG. Data collected between January 1st - June 30th 20ll from 23 subjects with obesity and T2DM were analyzed retrospectively. The following parameters recorded before, one and three months after surgery were compared: body mass index (BMI), fasting plasma glucose, lipid profile, arterial blood pressure, hypoglycemic drugs, antihypertensive and hypolipidemic drugs. Mean BMI decreased from 45.17 ± 8.342kg/m2 to 39 ± 6.031kg/m2 at one month and to 34.91 ± 4.435kg/m2 3 months after surgery. Fasting plasma glucose decreased to 117.87 ± 35.143mg/dl at one month and to 102.22 ± 16.605mg/dl, 3 months after surgery. Following surgery, afull remission ofT2DM was achieved in 39% of patients in the first month and in 65% of patients at 3 months. There was also an important improved in lipid profile, blood pressure control and obstructive sleep apnea (OSA). Our study has shown positive early effects of LSG on obese T2DM patients regarding weight loss and obesity - related comorbidities reduction. © 2011 ILEX PUBLISHING HOUSE, Bucharest, Roumania.

Uyy E.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Suica V.I.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Boteanu R.M.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Manda D.,National Institute Of Endocrinology Ci Parhon | And 4 more authors.
Journal of Proteome Research | Year: 2016

The study aimed to evaluate the proteomic changes in benign follicular adenoma versus malignant follicular variant of papillary thyroid carcinoma. Tumor and nontumor adjacent samples were analyzed by liquid nanochromatography mass spectrometry, and protein abundance was evaluated by label-free quantification. Western blotting and quantitative real-time polymerase chain reaction were used to validate and complement the mass spectrometry data. The results demonstrated deregulated expression of four endoplasmic reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin, calnexin, protein disulfide-isomerase A4), glutathione peroxidase 3 and thyroglobulin, all of them involved in thyroid hormone synthesis pathway. The altered tissue abundance of endoplasmic reticulum chaperones in thyroid cancer was correlated with serum expression levels. The identified proteins significantly discriminate between adenoma and carcinoma in both thyroid tissue and corresponding sera. Data are available via ProteomeXchange with identifier PXD004322. © 2016 American Chemical Society.

Manolescu B.N.,University of Sfax | Berteanu M.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,Universitary Emergency Hospital Elias | Oprea E.,University of Bucharest | And 6 more authors.
Annals of Clinical Biochemistry | Year: 2011

Background: Stroke patients have a redox imbalance, a consequence of both the cerebrovascular event and the associated pathological conditions. Our study was aimed to investigate the dynamic of some oxidative and nitrosative markers during the convalescent phase of postacute stroke patients undergoing rehabilitation. Methods: We assessed thiol, advanced oxidation protein product, protein carbonyl, 3-nitro-L-tyrosine, ceruloplasmin and oxidized LDL concentrations, as well as gamma-glutamyltranspeptidase (GGT) activity in 20 patients at the beginning of the hospitalization and at the discharge moment, respectively, and 24 apparently healthy controls. Results: We found significantly increased values for GGT (P = 0.04), ceruloplasmin (P = 0.01) and protein carbonyl (P = 0.04) in stroke patients at the hospitalization moment when compared with healthy controls, while total thiols were significantly decreased (P = 0.002). Rehabilitation was associated with a significant decrease of protein carbonyl (P = 0.03) and oxidized LDL particle concentrations (P = 0.03), as well as GGT activity (P = 0.02). At the hospitalization moment, both GGT and ceruloplasmin were significantly negatively correlated with non-proteic thiols (r = 20.44, P = 0.049, and r = 20.53, P = 0.015, respectively) and significantly positively with protein carbonyls (r = +0.80, P<0.001, and r = +0.69, P<0.001, respectively) suggesting putative roles of GGT and ceruloplasmin in the redox imbalance. Conclusions: These results highlight the existence of a redox imbalance in postacute stroke patients, and the possible benefits of an antioxidant-based therapy for the recovery of these patients.

Moanta M.L.,Carol Davila University of Medicine and Pharmacy | Moanta M.L.,Emergency Clinical County Hospital | Mota M.,Emergency Clinical County Hospital | Mota M.,University of Medicine and Pharmacy of Craiova | And 4 more authors.
Romanian Journal of Diabetes, Nutrition and Metabolic Diseases | Year: 2012

Objectives: To determine and compare the efficacy of two weight-reducing surgical interventions, gastric bypass (GBP) and sleeve gastrectomy (SG), in inducing remission/improvement of type 2 diabetes mellitus (T2DM). Materials and Methods: Data were collected from 162 obese T2DM subjects who underwent SG (96) or GBP (66) between 2009 and 2011. The following parameters were recorded: body mass index, waist-hip-ratio, blood pressure, fasting plasma glucose, HbA1c, lipid profile, hypoglycemic drugs used. Results: Remission of T2DM was achieved in 83% of patients who underwent SG and in 81% of patients who underwent GBP. For both procedures, T2DM remission rate was higher for patients with a shorter duration of T2DM, with better preoperative glycemic control and for those not requiring pharmacological agents before surgery. Conclusions: SG and GBP are effective in inducing T2DM remission in obese patients. The clinical features of T2DM are important predictors for the remission of the disease after bariatric surgery. © 2012 ILEX PUBLISHING HOUSE, Bucharest, Roumania.

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