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Nicolae I.,Dermatovenerological Center | Schipor S.,National Institute Of Endocrinology Ci Parhon
Journal of the European Academy of Dermatology and Venereology | Year: 2010

Background Recently, it became more evident that skin is a target for neuroendocrine signals. Aims (1) To evaluate the relationship between tumour aggressiveness and hypercalcaemia in patients with non-melanoma skin cancer; (2) to identify clinical, functional, biological alterations caused by this setting; (3) calcium redistribution from extracellular fluids to intracellular compartments; (4) to describe several molecular aspects of hypercalcaemia development. Materials and methods This study was conducted between January 2000 and May 2009 in Dermatoveneorological Center, Bucharest. From the 1232 cases that were investigated, there were 32 patients with keratoachantoma, 468 patients with basal cell carcinoma, 412 patients with squamous cell carcinoma and 320 healthy volunteers. All the patients were screened by clinical and paraclinical examinations (haematology, biochemistry, immunology). After biochemical confirmation of hypercalcaemia, patients had endocrine tests, electrocardiography and imagistic approaches. Total serum calcium was measured in extracellular fluids (serum, urine) by spectrophotometric methods. Ionized calcium was calculated depending on total serum calcium and total proteins. Corrected serum total calcium (cTCa) levels were calculated using albumin and total serum calcium levels. In tumour tissues and intact skin, calcium was assayed by physical methods of analysis: Instrumental Neutron Activation Analysis (INAA), Proton-Induced X-ray Emission (PIXE). Intact PTH was measured by ELISA. Results PTH-independent hypercalcaemia prevalence is low in SCC patients (1.21%). Hypercalcaemia manifestations are multiple including: digestive, renal, neuromuscular, and cardiovascular abnormalities. In these patients, intact PTH (iPTH) is normal, urinary calcium is decreased, serum albumin is reduced, and calcium concentration in tumour tissue is significantly increased compared to healthy tissue. Conclusions PTH-independent hypercalcaemia has a low prevalence in SCC patients. Hypercalcaemia is correlated with susceptibility to develop metastases in SCC. A possible mechanism is PTHrp hypersecretion by malignant keratinocytes. © 2009 European Academy of Dermatology and Venereology.

Georgescu M.,Carol Davila University of Medicine and Pharmacy | Tampa M.,Carol Davila University of Medicine and Pharmacy | Matei C.,Carol Davila University of Medicine and Pharmacy | Paun D.L.,National Institute Of Endocrinology Ci Parhon
Gineco.eu | Year: 2014

Important changes take place during pregnancy. Electrolyte imbalance and hormone fluctuations are recognised as triggers for neurological impairment, also commonly found in otorhinolaryngology. After delivery most of these deficits spontaneously remit. Still, due to ethical considerations treatment is mandatory in order to facilitate recovery of the neurological deficit. This case report presents a 33-year pregnant woman suffering from sudden unilateral sensorineural hearing loss and tinnitus in the third trimester. Although Dextrane 40 infusion treatment was successful, with measurable improvement on the pure tone audiogram, pharmacological treatment during pregnancy has to be carefully and individually coordinated. An interdisciplinary approach in diagnostic and therapeutic measures together with gynaecologists appears obligatory.

Luca C.,University of Bucharest | Buburuzan L.,University of Bucharest | Schipor S.,National Institute Of Endocrinology Ci Parhon | Badiu C.,National Institute Of Endocrinology Ci Parhon | And 3 more authors.
Annals of the Romanian Society for Cell Biology | Year: 2011

Mutations in genes encoding for SDHB and SDHD are identified as genetic causes of paraganglioma (PG) and pheochromocytoma (PC). Mutations of both genes are associated with relatively high rates of extra-adrenal tumors compared with adrenal tumors; however, the mutations in SDHB appear to be associated with more aggressive tumor behavior and a higher rate of malignancy. In this study we assessed the presence of different mutations in the SDHB gene from patients with sporadic PC. Blood samples from six patients were used for DNA extraction. Exons 3 to 7 of the SDHB gene were amplified by PCR using specific primers and analyzed by direct sequencing. We identified novel heterozygous point mutations in two patients with pheochromocytoma in intron two (T/G, + 25990) and intron four (C/A +30654, G/A +30831). Further studies will investigate the oncogenic implication of these mutations.

Manolescu B.N.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,University of Bucharest | Dumitru L.,Carol Davila University of Medicine and Pharmacy | And 9 more authors.
Inflammation | Year: 2011

Stroke is a pathological condition associated with an enhanced inflammatory response that has a multifactorial etiology. We evaluated the dynamic of plasma concentrations of IL-1α, IL-6, IL-8, TNF-α, soluble form of intercellular adhesion molecule 1, and lipoprotein (a) [Lp(a)] during the rehabilitation of post-acute stroke patients (n=20), in parallel with control subjects (n = 24). Stroke patients had significantly increased concentrations of IL-6, TNF-α, and Lp(a) when compared to healthy controls. It was found that the changes in the IL-6, IL-8, and TNF-α concentrations associated with the pathological condition were statistically significant (χ 2=4.81, p=0.028, χ 2=10.40, p=0.005 and χ 2=6.73, p=0.034, respectively). The decrease of Lp(a) during the rehabilitation had statistical significance (p = 0.043), while the decrease of IL-1α had marginal significance (p = 0.071). IL-1α, TNF-α, and Lp(a) concentrations were significantly negatively correlated with the Barthel index values, suggesting that the decrease of these inflammatory markers was beneficial for patients' recovery. © 2010 Springer Science+Business Media, LLC.

Moanta M.L.,Emergency Clinical County Hospital Craiova | Dinu I.-R.,Emergency Clinical County Hospital Craiova | Mota M.,University of Medicine and Pharmacy of Craiova | Copaescu C.,Delta Hospital Bucharest National Institute of Endocrinology CI Parhon | Dumitrache C.,National Institute of Endocrinology CI Parhon
Romanian Journal of Diabetes, Nutrition and Metabolic Diseases | Year: 2011

Obesity has become a serious health problem and it is a major risk factor for the development of insulin resistance and type 2 diabetes mellitus (T2DM). Laparoscopic sleeve gastrectomy (LSG) has revealed to be effective for achieving weight loss and apotential competitor with other operations. The aim of this study is to evaluate weight loss and the reversibility of associated comorbidities in obese T2DMpatients, one and three months after LSG. Data collected between January 1st - June 30th 20ll from 23 subjects with obesity and T2DM were analyzed retrospectively. The following parameters recorded before, one and three months after surgery were compared: body mass index (BMI), fasting plasma glucose, lipid profile, arterial blood pressure, hypoglycemic drugs, antihypertensive and hypolipidemic drugs. Mean BMI decreased from 45.17 ± 8.342kg/m2 to 39 ± 6.031kg/m2 at one month and to 34.91 ± 4.435kg/m2 3 months after surgery. Fasting plasma glucose decreased to 117.87 ± 35.143mg/dl at one month and to 102.22 ± 16.605mg/dl, 3 months after surgery. Following surgery, afull remission ofT2DM was achieved in 39% of patients in the first month and in 65% of patients at 3 months. There was also an important improved in lipid profile, blood pressure control and obstructive sleep apnea (OSA). Our study has shown positive early effects of LSG on obese T2DM patients regarding weight loss and obesity - related comorbidities reduction. © 2011 ILEX PUBLISHING HOUSE, Bucharest, Roumania.

Uyy E.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Suica V.I.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Boteanu R.M.,Institute of Cellular Biology and Pathology Nicolae Simionescu | Manda D.,National Institute Of Endocrinology Ci Parhon | And 4 more authors.
Journal of Proteome Research | Year: 2016

The study aimed to evaluate the proteomic changes in benign follicular adenoma versus malignant follicular variant of papillary thyroid carcinoma. Tumor and nontumor adjacent samples were analyzed by liquid nanochromatography mass spectrometry, and protein abundance was evaluated by label-free quantification. Western blotting and quantitative real-time polymerase chain reaction were used to validate and complement the mass spectrometry data. The results demonstrated deregulated expression of four endoplasmic reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin, calnexin, protein disulfide-isomerase A4), glutathione peroxidase 3 and thyroglobulin, all of them involved in thyroid hormone synthesis pathway. The altered tissue abundance of endoplasmic reticulum chaperones in thyroid cancer was correlated with serum expression levels. The identified proteins significantly discriminate between adenoma and carcinoma in both thyroid tissue and corresponding sera. Data are available via ProteomeXchange with identifier PXD004322. © 2016 American Chemical Society.

Manolescu B.N.,University of Sfax | Berteanu M.,Carol Davila University of Medicine and Pharmacy | Berteanu M.,Universitary Emergency Hospital Elias | Oprea E.,University of Bucharest | And 6 more authors.
Annals of Clinical Biochemistry | Year: 2011

Background: Stroke patients have a redox imbalance, a consequence of both the cerebrovascular event and the associated pathological conditions. Our study was aimed to investigate the dynamic of some oxidative and nitrosative markers during the convalescent phase of postacute stroke patients undergoing rehabilitation. Methods: We assessed thiol, advanced oxidation protein product, protein carbonyl, 3-nitro-L-tyrosine, ceruloplasmin and oxidized LDL concentrations, as well as gamma-glutamyltranspeptidase (GGT) activity in 20 patients at the beginning of the hospitalization and at the discharge moment, respectively, and 24 apparently healthy controls. Results: We found significantly increased values for GGT (P = 0.04), ceruloplasmin (P = 0.01) and protein carbonyl (P = 0.04) in stroke patients at the hospitalization moment when compared with healthy controls, while total thiols were significantly decreased (P = 0.002). Rehabilitation was associated with a significant decrease of protein carbonyl (P = 0.03) and oxidized LDL particle concentrations (P = 0.03), as well as GGT activity (P = 0.02). At the hospitalization moment, both GGT and ceruloplasmin were significantly negatively correlated with non-proteic thiols (r = 20.44, P = 0.049, and r = 20.53, P = 0.015, respectively) and significantly positively with protein carbonyls (r = +0.80, P<0.001, and r = +0.69, P<0.001, respectively) suggesting putative roles of GGT and ceruloplasmin in the redox imbalance. Conclusions: These results highlight the existence of a redox imbalance in postacute stroke patients, and the possible benefits of an antioxidant-based therapy for the recovery of these patients.

Moanta M.L.,Carol Davila University of Medicine and Pharmacy | Moanta M.L.,Emergency Clinical County Hospital | Mota M.,Emergency Clinical County Hospital | Mota M.,University of Medicine and Pharmacy of Craiova | And 4 more authors.
Romanian Journal of Diabetes, Nutrition and Metabolic Diseases | Year: 2012

Objectives: To determine and compare the efficacy of two weight-reducing surgical interventions, gastric bypass (GBP) and sleeve gastrectomy (SG), in inducing remission/improvement of type 2 diabetes mellitus (T2DM). Materials and Methods: Data were collected from 162 obese T2DM subjects who underwent SG (96) or GBP (66) between 2009 and 2011. The following parameters were recorded: body mass index, waist-hip-ratio, blood pressure, fasting plasma glucose, HbA1c, lipid profile, hypoglycemic drugs used. Results: Remission of T2DM was achieved in 83% of patients who underwent SG and in 81% of patients who underwent GBP. For both procedures, T2DM remission rate was higher for patients with a shorter duration of T2DM, with better preoperative glycemic control and for those not requiring pharmacological agents before surgery. Conclusions: SG and GBP are effective in inducing T2DM remission in obese patients. The clinical features of T2DM are important predictors for the remission of the disease after bariatric surgery. © 2012 ILEX PUBLISHING HOUSE, Bucharest, Roumania.

Maria I.A.,Carol Davila University of Medicine and Pharmacy | Cristina D.,Genetic Laboratory | Emilia S.,Carol Davila University of Medicine and Pharmacy | Schipor S.,National Institute Of Endocrinology Ci Parhon | And 8 more authors.
Romanian Biotechnological Letters | Year: 2016

Introduction: The aim of this study was to develop a noninvasive method for fetal RHD genotyping by analyzing cell free fetal DNA circulating in the maternal blood in order to help improve the management of the mother-fetus Rh incompatibility. Materials and Methods: Total cell free DNA from maternal blood samples was extracted manually. In order to detect the targeted sequences, we develop two distinct end-point multiplex PCR reactions followed by automatic high-resolution capillary electrophoresis. Results and Discussions: During our study, one hundred and thirty-six plasma samples withdrawn from women in their 5th to 28th weeks of pregnancy were tested. We evaluated the diagnostic accuracy of this noninvasive fetal RHD genotyping approach and found that we successfully genotyped one hundred and thirty-five (97.8%) samples. Three samples (2.2%) were inconclusive due to small amount of fetal DNA in maternal plasma, correlated with blood sampling too early in pregnancy (5th to 7th weeks). After the 9th week of pregnancy, we were able to retest only two of three samples, one sample being impossible to test because of a miscarriage. Conclusion: The results presented in this paper are demonstrating the feasibility of this protocol to correctly genotype the RHD gene and its potential value for the Romanian prenatal care, screening and diagnosis services. © 2016 University of Bucharest.

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