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Chuzhou, China

Zhu L.,Anhui Normal University | Zhu L.,Chuzhou College | Wu X.,Anhui Normal University | Wu C.,CAS Institute of Zoology
Oriental Insects

The mitochondrial DNA (mtDNA) variation in Papilio bianor Cramer from its extant range was evaluated to provide a phylogeographic history of its populations from China. Molecular variation in a 655 bp section of COII from 128 individuals (22 populations) was analysed, and 17 haplotypes were detected. Phylogenetic analysis revealed the presence of three major lineages, northeastern, southwestern and central lineages. The sequence divergence suggests that these regions had been isolated since the early Pleistocene. Nested clade, coalescent and genetic structure analyses indicate that allopatric fragmentation might be the cause of the deep divergence within the species. This period of allopatry was followed by contiguous range expansion, presumably involving recolonisation of formerly glaciated or uninhabitable areas from at least three refuges. © 2011 Taylor & Francis. Source

Guo L.,Chuzhou College | Fang P.,Nanjing University | Yu M.,Nanjing University | Shi M.,Yangzhou University | And 2 more authors.
Journal of Endocrinology

Alarin, a regulatory peptide, belongs to the galanin family and plays the same regulatory roles as galanin in orexigenic activity and energy metabolism. Our previous studies had found that galanin might facilitate insulin sensitivity via activation of its central receptors. To date, little is known about whether central alarin may exert similar effects on insulin sensitivity. In order to investigate this, alarin and its specific antagonist, alarin 6-25Cys, were administered into the cerebral ventricles of type 2 diabetic rats (T2DR) to evaluate the changes in insulin resistance. The results indicated that central treatment with alarin significantly increased the body weight of animals, the 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose uptake, the plasma adiponectin levels, the glucose infusion rates in hyperinsulinemic-euglycemic clamp tests, the vesicle-associated membrane protein 2 as well as glucose transporter 4 (GLUT4 (SLC2A4)) protein and mRNA levels, and the ratios of GLUT4 contents in plasma membranes to total cell membranes in adipocytes, but reduced blood glucose and plasma retinol-binding protein 4 levels. These effects of alarin may be inhibited by pretreatment with alarin 6-25Cys. The above-mentioned results suggest that the central alarin projective system may facilitate insulin sensitivity and glucose uptake via the increase in GLUT4 content and GLUT4 translocation from intracellular pools to plasma membranes in T2DR. © 2014 Society for Endocrinology. Source

Li Q.,Nanjing University | Li Q.,Chuzhou College
International Journal of Computer Mathematics

In this paper, we present a class of non-conventional two-step explicit methods for solving the second-order initial value problems (IVPs) with periodic or oscillatory solutions. The methods have second algebraic order, a large interval of periodicity and high phase-lag order. For multi-dimensional problems, we give the vector form of the methods with the aid of a special vector operation. Some numerical results are reported to illustrate the efficiency of our methods. Source

Guo L.,Chuzhou College | Shi M.,Yangzhou University | Zhang L.,Yangzhou University | Li G.,Yangzhou University | And 6 more authors.
General and Comparative Endocrinology

Seeing that galanin increases animal body weight on the conditions of inhibiting insulin secretion and animals with metabolic disorder of galanin easily suffer from diabetes, we postulate that endogenous galanin is necessary to reduce insulin resistance in adipocytes. To test this hypothesis, we compared four groups of rats to examine whether an increase in galanin secretion stimulated by swimming may reduce insulin resistance. The rats from sedentary and trained drug groups were injected by M35, a galanin antagonist. The rats from trained control and trained drug groups swam after each injection for four weeks. We found that exercise significantly elevated plasma galanin contents and glucose transporter 4 (GLUT4) mRNA levels in adipocytes. Meanwhile, M35 treatment reduced GLUT4 and GLUT4 mRNA levels, and glucose infusing rates in euglycemic-hyperinsulinemic clamp tests. The ratios of GLUT4 concentrations at plasma membranes to total cell membranes in both drug groups were lower compared with each control group, respectively. These observations suggest that endogenous galanin reduces insulin resistance by increasing GLUT4 contents and promoting GLUT4 transportation from intracellular membranes to plasma membranes in adipocytes. Galanin is an important hormone to reduce insulin resistance in rats. © 2011 Elsevier Inc. Source

Zhang Z.,Yangzhou University | Guo L.,Chuzhou College | Li G.,Yangzhou University | Zhang L.,Yangzhou University | And 3 more authors.
Molecular and Cellular Endocrinology

The aim of this study is to investigate whether galanin (GAL) central receptors are involved in regulation of insulin resistance. To test it, a GAL antagonist, M35 was intracerebroventricularly administrated in trained type 2 diabetic rats. The euglycemic-hyperinsulinemic clamp test was conducted for an index of glucose infusion rates. The epididymal fat pads were processed for determination of glucose uptake and Glucose Transporter 4 (GLUT4) amounts. The Gal mRNA expression levels in hypothalamus were quantitatively assessed too. We found an inhibitory effect of M35 on glucose uptake into adipocytes, Gal mRNA expression levels in hypothalamus, glucose infusion rates in the clamp test and GLUT4 concentration in plasma membranes and total cell membranes of adipocytes. The ratios of GLUT4 contents of the former to the latter in M35 groups were lower. These results suggest a facilitating role for GAL on GLUT4 translocation and insulin sensitivity via its central receptors in rats. © 2012 Elsevier Ireland Ltd. Source

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