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Shen G.,University of Science and Arts of Iran | Cai C.,Chuxiong Normal University | Yang J.,University of Science and Arts of Iran
Electrochimica Acta | Year: 2011

A novel electrochemical immunosensor based on gold-hydroxyapatite nanocomposite-chitosan film has been developed for the detection of prostate-specific antigen. The large surface area of the gold-hydroxyapatite nanocomposite and the porous morphology of chitosan allowed high antibody loading without the loss of bioactivity. The stepwise modification of the electrode was characterized with cyclic voltammetry. Different parameters, including the pH of a supporting electrolyte, the size of the gold nanoparticles, and the antibody concentration were optimized. Under the optimal conditions, the electrochemical immunosensor reliably detected prostate-specific antigen at concentrations from 3.5 to 30 ng/mL with a detection threshold of 2.6 ng/mL. The prepared biosensor was used to assay prostate-specific antigen in human serum samples, and the results were in good agreement with those obtained by enzyme-linked immunosorbent assays. The reported immunosensor exhibits rapid detection, good reproducibility, high sensitivity, specificity, and stable performance. The proposed strategy can be extended for the development of other biosensors. © 2011 Elsevier Ltd. Source


Huang Y.,Chuxiong Normal University
Nonlinear Dynamics | Year: 2013

With the aid of the known Bäcklund transformation, starting from some given traveling solutions, we consider new exact no-traveling wave solutions to the Liouville equation, and a series of breather soliton solutions, doubly periodic solutions, two-soliton solutions as well as periodic-soliton solutions are obtained. © 2012 Springer Science+Business Media Dordrecht. Source


Huang Y.,Chuxiong Normal University
Nonlinear Dynamics | Year: 2014

By means of the known Darboux transformation, starting from some stationary wave solutions, we construct a large number of new explicit multiple waves solutions to the Korteweg-de Vries equation, including stationary periodic-soliton solutions, stationary soliton-periodic solutions, doubly periodic solutions, triply periodic solutions, as well as two- and three-soliton solutions. © 2014 Springer Science+Business Media Dordrecht. Source


Qin H.-F.,Chuxiong Normal University
Research Journal of Applied Sciences, Engineering and Technology | Year: 2012

This study analyzes telecom owe churn customers. Firstly, use the technology of OLAP to analyze the whole theme of the loss of arrears. Then, adopting cluster analysis analyzed consumer behavior (traffic, consumption amount, owed amount) before churn of the churn customer, age and in the net time and explaining the cluster. Finally, using the decision tree analyze the discrete variable and draws the conclusion. © Maxwell Scientific Organization 2012. Source


Shen G.,University of Science and Arts of Iran | Cai C.,Chuxiong Normal University | Wang K.,University of Science and Arts of Iran | Lu J.,University of Science and Arts of Iran
Analytical Biochemistry | Year: 2011

For the construction of a well-defined antibody surface, protein A was used as a binding material to immobilize antibodies onto gold-derivatized transducers. The traditional method tends to assemble protein A directly onto the gold-derivatized transducers. In this paper, we tried to indirectly bind protein A onto sensors through hyperbranched polymer (HBP) which was synthesized from p-phenylenediamine and trimesic acid. The three-dimensional structure of HBP and the characteristics including orientation control and biocompatibility of protein A led to highly efficient immunoreactions and enhanced detection system performance. With this strategy, cysteamine monolayer was first assembled onto Au electrodes associated with the piezoelectric quartz crystal; secondly, the cysteamine-modified gold electrode was further modified by the activated HBP; thirdly, protein A was immobilized onto the HBP film; and finally, antibodies were immobilized onto the surface of protein A film for detecting the corresponding antigen. The quartz crystal microbalance immunosensor thus fabricated was applied to detect hepatitis B surface antigen in solutions that ranged from 0.71 to 300 μg mL -1. The detection limit was estimated to be 0.53 μg mL -1. The immunosensor holds good selectivity, sensitivity, and repeatability. © 2010 Elsevier Inc. All rights reserved. Source

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