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Song J.-H.,Kangwon National University | Park K.,Chungcheongnam Do Institute of Health and Environment Research | Shim A.,Kangwon National University | Kwon B.-E.,Kangwon National University | And 6 more authors.
Osong Public Health and Research Perspectives | Year: 2015

Objectives: Coxsackievirus A group 16 strain (CVA16) is one of the predominant causative agents of hand, foot, and mouth disease (HFMD). Methods: Using a specimen from a male patient with HFMD, we isolated and performed sequencing of the Korean CVA16 strain and compared it with a G10 reference strain. Also, we were investigated the effects of medicinal plant extract on the cytopathic effects (CPE) by CPE reduction assay against Korean CVA16. Results: Phylogenetic analysis showed that the Korean CVA16 isolate belonged to cluster B-1 and was closely related to the strain PM-15765-00 isolated in Malaysia in 2000. The Korean CVA16 isolate showed 73.2% nucleotide identity to the G10 prototype strain and 98.7% nucleotide identity to PM-15765-00. Next, we assessed whether the Korean CVA16 isolate could be used for invitro screening of antiviral agents to treat HFMD infection. Vero cells infected with the Korean CVA16 isolate showed a cytopathic effect 2 days after the infection, and the treatment of cells with Cornus officinalis, Acer triflorum, Pulsatilla koreana, and Clematis heracleifolia var. davidiana Hemsl extracts exhibited strong antiviral activity against CVA16. Conclusion: Collectively, our work provides potential candidates for the development of vaccine and novel drugs to treat the CVA16 strain isolated from a Korean patient. © 2015 Korea Centers for Disease Control and Prevention. Source

Kim Y.-I.,Ajou University | Song J.-H.,Kangwon National University | Kwon B.-E.,Kangwon National University | Kim H.-N.,Ajou University | And 7 more authors.
Vaccine | Year: 2015

Enterovirus 71 (EV71) causes hand, foot, and mouth diseases and can result in severe neurological disorders when it infects the central nervous system. Thus, there is a need for the development of effective vaccines against EV71 infection. Here we report that viral capsid protein 1 (VP1), one of the main capsid proteins of EV71, efficiently elicited VP1-specific immunoglobulin G (IgG) in the serum of mice immunized with recombinant VP1. The VP1-specific IgG produced in female mice was efficiently transferred to their offspring, conferring protection against EV71 infection immediately after birth. VP1-specific antibody can neutralize EV71 infection and protect host cells. VP1-specific maternal IgG in offspring was maintained for over 6 months. However, the pre-existence of VP1-specific maternal IgG interfered with the production of VP1-specific IgG antibody secreting cells by active immunization in offspring. Therefore, although our results showed the potential for VP1-specific maternal IgG protection against EV71 in neonatal mice, other strategies must be developed to overcome the hindrance of maternal IgG in active immunization. In this study, we developed an effective and feasible animal model to evaluate the protective efficacy of humoral immunity against EV71 infection using a maternal immunity concept. © 2015 Elsevier Ltd. Source

Kim D.-U.,Chungcheongnam Do Institute of Health and Environment Research | Kim D.-U.,Chungbuk National University | Yeo S.-G.,National Health Research Institute | Baek K.-A.,Chungcheongnam Do Institute of Health and Environment Research | And 7 more authors.
Journal of Pure and Applied Microbiology | Year: 2014

With the increase in population visiting hot water systems such as spas and water parks, the opportunity to be exposed to the risk of legionellae's infection is rising in Chungnam, Korea. So, we investigated Legionella contamination and its population on environmental sources for assessing the relationships in the types of facilities and water sources. PCR and culture method were used to detect and isolate Legionella spp. Partial 16s rRNA and rpoB gene sequences were used to identify the isolates and determine subspecies of Legionella pneumophila isolates. The detection rate (29%) of Legionella spp.from public bathes was the highest and L. pneumophila was the dominant species in Chungnam, Korea. L. pneumophila isolates used in this study were divided into subsp. pneumophila and subsp.fraseri. All of Legionella species isolated in this study except for L. nautamm were pathogenic to human. L. pneumophila isolates used in this study were divided into subsp. pneumophila and subsp.fraseri. Results of identification between rpoB and 16s rRNA trees were nearly consistent, except for some isolates. Our findings will be helpful to create a better understanding of the molecular basis of Legionella and further preventing and controlling outbreaks by legionellosis. Source

Park K.S.,Chungcheongnam Do Institute of Health and Environment Research | Kang K.H.,Chungcheongnam Do Fishery Institute Freshwater Fishery Development Center | Bae E.Y.,Elohim Co. R and D center | Baek K.A.,Chungcheongnam Do Institute of Health and Environment Research | And 6 more authors.
International Food Research Journal | Year: 2015

Our study was undertaken to gain basic and detailed information about the composition of caviar from sterlet (Acipenser ruthenus) aqua-cultured in Korea. The moisture, protein, and lipid content of caviar samples was 51.32 ± 2.36%, 25.43 % ± 0.32, and 13.21% ± 0.44, respectively. Hunter color L, a, and b value of unprocessed caviars was 14.83, 0.58, and 1.87, respectively. In accordance with time distribution, a and b values were not significantly changed during the period of observation, but L values indicative of brightness began to decline from 5 weeks. The fatty acids of the caviars consisted of saturated (27.87%), monounsaturated (43.14%), and polyunsaturated fatty acids (28.99%), especially, omega-3 fatty acids such as docosahexaenoic acid (11.39%) and eicosapentanoic acid (4.69%). Among the total amino acids, glutamic acid of 33.5 mg/g (13.9%) was the largest portion of caviars, followed by lysine (27.1 mg/g, 11.3%), leucine (23.1 mg/g, 9.6%), and phenylalanine (22.4 mg/g, 9.3%). © All Rights Reserved. Source

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