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Yoon M.-Y.,Kongju National University | Moe K.T.,Kongju National University | Kim D.-Y.,South Korean National Institute of Animal Science | Rho I.-R.,South Korean National Institute of Animal Science | And 5 more authors.
Electronic Journal of Biotechnology

In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a single population in which > 70% of their inferred ancestry was derived from one of the model-based populations. However, two accessions (3.4%) in the sample were categorized as having admixed ancestry. Here, we report detailed information on commercially grown strawberry accessions from five different origins using SSR markers. These results couldbe used for broadening the genetic base of commercially grown varieties. © 2012 by Pontificia Universidad Católica de Valparaíso, Chile. Source

Lee C.H.,Chungnam National University | Min J.H.,Chungnam National University | Kim T.I.L.,Chungcheongnam do Agricultural Research and Extension Services | Kim J.G.,South Korean National Institute of Animal Science | And 3 more authors.
Horticulture Environment and Biotechnology

Three local varieties of related strawberries, were examined to find the relationship between fruit firmness and cell wall matrix. Firmness loss between the white to full ripe stage in firm variety, 'Maehyang' was lower and contained higher amount of ethanol insoluble solids (EIS) at full ripe stage. Firmness difference was not found at full ripe stage between soft varieties, 'Akihime' and 'Seohyang', even 'Akihime' had a significantly higher amount of ethanol insoluble solids than 'Seolhyang'. The water soluble pectin of 'Maehyang' occupied about 54. 1% of total pectins whereas it was 63. 7% and 62. 5% in 'Akihime 'and 'Seolhyang', respectively. Difference in cellulose contents was not found between varieties and fruit developmental stages. The level of total neutral sugars of EIS was much lower in 'Seolhyang' compared to the other varieties. Arabinose showed a decreasing trend regardless of varieties along with the advance of ripening. There was an increase of galactose contents in 'Seolhyang' unlike in the other varieties. Gel chromatography elution profiles of wall polysaccharide showed no clear relationships between fruit firmness and depolymerization. © 2011 Korean Society for Horticultural Science. Source

Jang J.-O.,Korea Research Institute of Bioscience and Biotechnology | Jang J.-O.,Chungnam National University | Kwon M.-K.,Chungcheongnam do Agricultural Research and Extension Services | Park D.-J.,Korea Research Institute of Bioscience and Biotechnology | And 2 more authors.
Journal of Applied Biological Chemistry

This study was carried out to investigate the plant growth promoting activity of hairy vetch (Vicia villosa Roth) on reclaimed land. At the previous research, Rhizobium sp. RH84 was isolated and selected for further study from hairy vetch. For the investigation of plant growth promoting effects by the Rhizobium sp. RH84, production of indole acetic acid (IAA), siderophore, phosphate solubilization and nitrogen fixation were tested and other characters were examined. As results, RH84 produced 9.03 μg IAA per mL and showed nitrogen fixation activity. With the treatment of Rhizobium sp. RH84 to hairy vetch showed good growth at 0.3% salty reclaimed soil, and the production yield was increased up to 56% at field test. From these results, it was confirmed that the Rhizobium sp. RH84 would be used as a green manure for hairy vetch under the salty condition of reclaimed land. © The Korean Society for Applied Biological Chemistry 2013. Source

Min S.R.,Korea Research Institute of Bioscience and Biotechnology | Ko S.M.,Jeju National University | Lyu J.I.,Korea Research Institute of Bioscience and Biotechnology | Park J.H.,Korea Research Institute of Bioscience and Biotechnology | And 9 more authors.
Journal of Plant Biotechnology

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants. © Korean Society for Plant Biotechnology. Source

Park S.-Y.,South Korean National Institute of Animal Science | Park W.T.,Chungnam National University | Park Y.C.,Chungcheongnam do Agricultural Research and Extension Services | Ju J.I.,Chungcheongnam do Agricultural Research and Extension Services | And 2 more authors.
Bioscience, Biotechnology and Biochemistry

Lycium chinense has been used as a traditional medicine for centuries in Asia because of its positive effects on health. However, its functional components have not been elucidated. This study determines the levels of health-promoting lipophilic compounds, including carotenoids, tocopherols, and phytosterol, and those of 42 hydrophilic metabolites, including sugars, organic acids, alcohols, amines, and amino acids, in L. chinense fruit from 11 cultivars. The metabolite profiles were subjected to a principal component analysis (PCA), Pearson correlation analysis, and hierarchical clustering analysis (HCA). PCA showed the Cheongdang (LM-3) cultivar to be distinct from the others. The correlation results for a total of 55 compounds revealed strong correlations between the metabolites that participated on closely related pathways. The Cheongdang cultivar appears to be most suited for functional food production because of its high carotenoid, tocopherol, and phytosterol levels. These results indicate the usefulness of metabolite profiling as a tool for assessing the quality of food. Source

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