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PubMed | Biological Resources Research Center, Chungcheongnam do Agricultural Research and Extension Services, Sungkyunkwan University, Kyungpook National University and National Academy of Agricultural Science
Type: | Journal: Scientific reports | Year: 2016

Tomato yellow leaf curl virus (TYLCV) is one of the most well-known tomato-infecting begomoviruses and transmitted by Bemisia tabaci. Seed transmission has previously been reported for some RNA viruses, but TYLCV has not previously been described as a seed-borne virus. In 2013 and 2014, without whitefly-mediated transmission, TYLCV was detected in young tomato plants germinated from fallen fruits produced from TYLCV-infected tomato plants in the previous cultivation season. In addition, TYLCV-Israel (TYLCV-IL) was also detected in seeds and their seedlings of TYLCV-infected tomato plants that were infected by both viruliferous whitefly-mediated transmission and agro-inoculation. The seed infectivity was 20-100%, respectively, and the average transmission rate to seedlings was also 84.62% and 80.77%, respectively. TYLCV-tolerant tomatoes also produced TYLCV-infected seeds, but the amount of viral genome was less than seen in TYLCV-susceptible tomato plants. When tomato plants germinated from TYLCV-infected seeds, non-viruliferous whiteflies and healthy tomato plants were placed in an insect cage together, TYLCV was detected from whiteflies as well as receiver tomato plants six weeks later. Taken together, TYLCV-IL can be transmitted via seeds, and tomato plants germinated from TYLCV-infected seeds can be an inoculum source of TYLCV. This is the first report about TYLCV seed transmission in tomato.


Park S.-Y.,South Korean National Institute of Animal Science | Park W.T.,Chungnam National University | Park Y.C.,Chungcheongnam do Agricultural Research and Extension Services | Ju J.I.,Chungcheongnam do Agricultural Research and Extension Services | And 2 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2012

Lycium chinense has been used as a traditional medicine for centuries in Asia because of its positive effects on health. However, its functional components have not been elucidated. This study determines the levels of health-promoting lipophilic compounds, including carotenoids, tocopherols, and phytosterol, and those of 42 hydrophilic metabolites, including sugars, organic acids, alcohols, amines, and amino acids, in L. chinense fruit from 11 cultivars. The metabolite profiles were subjected to a principal component analysis (PCA), Pearson correlation analysis, and hierarchical clustering analysis (HCA). PCA showed the Cheongdang (LM-3) cultivar to be distinct from the others. The correlation results for a total of 55 compounds revealed strong correlations between the metabolites that participated on closely related pathways. The Cheongdang cultivar appears to be most suited for functional food production because of its high carotenoid, tocopherol, and phytosterol levels. These results indicate the usefulness of metabolite profiling as a tool for assessing the quality of food.


Lee C.H.,Chungnam National University | Min J.H.,Chungnam National University | Kim T.I.L.,Chungcheongnam do Agricultural Research and Extension Services | Kim J.G.,South Korean National Institute of Animal Science | And 3 more authors.
Horticulture Environment and Biotechnology | Year: 2011

Three local varieties of related strawberries, were examined to find the relationship between fruit firmness and cell wall matrix. Firmness loss between the white to full ripe stage in firm variety, 'Maehyang' was lower and contained higher amount of ethanol insoluble solids (EIS) at full ripe stage. Firmness difference was not found at full ripe stage between soft varieties, 'Akihime' and 'Seohyang', even 'Akihime' had a significantly higher amount of ethanol insoluble solids than 'Seolhyang'. The water soluble pectin of 'Maehyang' occupied about 54. 1% of total pectins whereas it was 63. 7% and 62. 5% in 'Akihime 'and 'Seolhyang', respectively. Difference in cellulose contents was not found between varieties and fruit developmental stages. The level of total neutral sugars of EIS was much lower in 'Seolhyang' compared to the other varieties. Arabinose showed a decreasing trend regardless of varieties along with the advance of ripening. There was an increase of galactose contents in 'Seolhyang' unlike in the other varieties. Gel chromatography elution profiles of wall polysaccharide showed no clear relationships between fruit firmness and depolymerization. © 2011 Korean Society for Horticultural Science.


Jang J.-O.,Korea Research Institute of Bioscience and Biotechnology | Jang J.-O.,Chungnam National University | Kwon M.-K.,Chungcheongnam do Agricultural Research and Extension Services | Park D.-J.,Korea Research Institute of Bioscience and Biotechnology | And 2 more authors.
Journal of Applied Biological Chemistry | Year: 2013

This study was carried out to investigate the plant growth promoting activity of hairy vetch (Vicia villosa Roth) on reclaimed land. At the previous research, Rhizobium sp. RH84 was isolated and selected for further study from hairy vetch. For the investigation of plant growth promoting effects by the Rhizobium sp. RH84, production of indole acetic acid (IAA), siderophore, phosphate solubilization and nitrogen fixation were tested and other characters were examined. As results, RH84 produced 9.03 μg IAA per mL and showed nitrogen fixation activity. With the treatment of Rhizobium sp. RH84 to hairy vetch showed good growth at 0.3% salty reclaimed soil, and the production yield was increased up to 56% at field test. From these results, it was confirmed that the Rhizobium sp. RH84 would be used as a green manure for hairy vetch under the salty condition of reclaimed land. © The Korean Society for Applied Biological Chemistry 2013.


Song J.Y.,Chungnam National University | Seo M.W.,Chungnam National University | Kim S.I.,Chungcheongnam do Agricultural Research and Extension Services | Nam M.H.,Chungcheongnam do Agricultural Research and Extension Services | And 2 more authors.
Mycobiology | Year: 2014

We analyzed the genetic diversity of Cylindrocarpon destructans isolates obtained from Korean ginseng (i.e., Panax ginseng) roots by performing virulence tests and nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA sequence analysis. The phylogenetic relationship analysis performed using ITS DNA sequences and isolates from other hosts helped confirm that all the Korean C. destructans isolates belonged to Nectria/Neonectria radicicola complex. The results of in vivo and ex vivo virulence tests showed that the C. destructans isolates could be divided into two groups according to their distinctive difference in virulence and the genetic diversity. The highly virulent Korean isolates in pathogenicity group II (PG II), together with foreign isolates from P. ginseng and P. quinquefolius, formed a single group. The weakly virulent isolates in pathogenicity group I, together with the foreign isolates from other host plants, formed another group and exhibited a greater genetic diversity than the isolates of PG II, as confirmed by the mt SSU rDNA sequence analysis. In addition, as the weakly virulent Korean isolates were genetically very similar to the foreign isolates from other hosts, they were likely to originate from hosts other than the ginseng plants. © The Korean Society of Mycology.


Kim H.-K.,Chungcheongnam do Agricultural Research and Extension Services | Yang E.-S.,Chungcheongnam do Agricultural Research and Extension Services | Park G.-M.,Sungkyunkwan University | Kim G.-H.,Geumsan Ginseng and Medicinal Crop Experiment Station | And 2 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2011

This study was conducted to investigate the quality characteristics, antioxidant activity, and α-glucoamylase inhibitory activity of Dahyang, a Chungnam Agricultural Research and Extension Service's newly bred cultivar of brown button mushroom. Total phenolic compound contents of Dahyang and the no. 705 mushroom were 189±12 mg% and 168±8 mg%, respectively. The major free sugars in Dahyang were mannitol (3.11%), xylose (0.12%), and trehalose (0.08%). β-Glucan content was 28.34% in Dahyang and 26.55% in the no. 705 mushroom, respectively. Electron donating ability by DPPH in Dahyang and the no. 705 mushroom was 52.14% and 45.27% for the water extract, and 57.81% and 46.93% for the 80% ethanol extract, respectively. α-Glucoamylase inhibitory activity in a 10 mg/mL concentration of water extract were was 33.25% in Dahyang and 29.22% in the no. 705 mushroom, respectively.


Yoon M.-Y.,Kongju National University | Moe K.T.,Kongju National University | Kim D.-Y.,South Korean National Institute of Animal Science | Rho I.-R.,South Korean National Institute of Animal Science | And 5 more authors.
Electronic Journal of Biotechnology | Year: 2012

In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a single population in which > 70% of their inferred ancestry was derived from one of the model-based populations. However, two accessions (3.4%) in the sample were categorized as having admixed ancestry. Here, we report detailed information on commercially grown strawberry accessions from five different origins using SSR markers. These results couldbe used for broadening the genetic base of commercially grown varieties. © 2012 by Pontificia Universidad Católica de Valparaíso, Chile.


Kim D.C.,Chungcheongnam do Agricultural Research and Extension Services | Choi H.G.,Chungcheongnam do Agricultural Research and Extension Services | Pak H.S.,Chungcheongnam do Agricultural Research and Extension Services | Lee Y.H.,Chungcheongnam do Agricultural Research and Extension Services | And 3 more authors.
Korean Journal of Horticultural Science and Technology | Year: 2015

The new garden chrysanthemum (Dendranthema grandiflorum Ramat.) cultivar ‘Nuri Ball’ was developed at Yesan Chrysanthemum Experiment Station of Chungcheongnam-do Agricultural Research and Extension Services in 2011. ‘Nuri Ball’ was bred through a cross between the ‘02-145-01’ line as the female parent with yellow flower color and ‘02-04-32’ as the male plant with white flower color in 2004. Three years of adaptation trials were conducted from 2006 to 2009 under natural conditions. This study compared the external shape type with that of ‘White Miri’ and conducted ploidy and RAPD (Random amplified polymorphic DNA) marker analyses. These tests showed that ‘Nuri Ball’ cultivar has its own characteristics compared with the control ‘White Miri’. ‘Nuri Ball’ was a shrub type variety with semi-double flowers of 4.0 cm in width with white petals. It could produce 1025.2 flowers per plant in autumn. Compared with the control ‘White Miri’, ‘Nuri Ball’ was similar in terms of shape and color of flowers, but was different in flower size and number. The natural flowering time of ‘Nuri Ball’ was late September. It had very vigorous growth and an early budding plant. ‘Nuri Ball’ was demonstrated to be a new cultivar based on ploidy test and RAPD analysis. ‘Nuri Ball’ is intended for use as a bed chrysanthemum and expected to contribute to farm incomes in landscaping. © 2015 Korean Society for Horticultural Science.


Nam M.H.,Chungcheongnam do Agricultural Research and Extension Services | Kim H.S.,Chungcheongnam do Agricultural Research and Extension Services | Choi J.H.,Chungcheongnam do Agricultural Research and Extension Services | Lee H.D.,Chungcheongnam do Agricultural Research and Extension Services
Korean Journal of Horticultural Science and Technology | Year: 2013

Fungicide applications are required to prevent the strawberry from Botrytis fruit rot and powdery mildew that infect open strawberry flowers, however, their effects of fungicides on pollen germination of strawberry have been rarely documented, particularly those from recently developed active fungicidal ingredients. In this study we have evaluated the effects of 24 commercial fungicidal formulations and 6 organic materials on pollen germination in 3 strawberry cultivars using in vitro assays. Pollens from strawberry had higher germination rates on agar with sucrose of 18% and 25 °C than other tested conditions. Pollen germination rates of cvs. Seolhyang, Maehyang, and Kumhyang at 18% sucrose and 25 °C were 15.3, 18.4 and 30.7%, respectively. Pyraclostrobin, azoxystrobin, kresoxim-methyl, dichlofluanid, iminoctadine tris, and sulfur showed the strongest inhibitory efficacy with the germination rates of more than 93.8% compared to the no-fungicide control. Germination was not significantly affected by simeconazole and procymidone. This in vitro germination study may provide information useful for selecting fungicides in flowering stage to strawberry farmers. © 2013 Korean Society for Horticultural Science.


Jo M.H.,Chungcheongnam do Agricultural Research and Extension Services | Ham I.K.,Chungcheongnam do Agricultural Research and Extension Services | Moe K.T.,Kongju National University | Kwon S.-W.,Kongju National University | And 6 more authors.
Australian Journal of Crop Science | Year: 2012

Garlic bulb is an important seasoning ingredient in many of the world's cuisines. However, clonal lineages within this species show a remarkably high degree of phenotypic diversity in bulb size and color. Present study classified the genetic variations of garlic. Seven selected simple sequence repeats (SSRs) revealed a total of 37 alleles across 120 garlic accessions, with an average of seven alleles per locus. The values for observed heterozygosity ranged from 0 to 0.99 (mean = 0.71). The average genetic diversity and polymorphic information content values were 0.586 and 0.518, respectively. Based on the 37 alleles obtained from the seven SSRs, a phylogram was constructed to understand the relationships among the 120 accessions. Thegarlic accessions were clustered into fourmaingroups (G1-G4)in the phylogram. Group1 consisted ofaccessionsof 'Aomori', Group2 consisted of64 accessions, Group3 consisted of25 accessions, and Group4 consisted of20accessions. Our results indicate that genetic diversity is correlated with geographical region. There may have been local selection pressure and differences in adaptability of the garlic to different geographical conditions. All of the tested loci deviated significantly (P < 0.01) from Hardy-Weinberg equilibrium. Thus, a number of disturbances occurred in the garlic population tested, including natural selection. Our findings will helpexplain the genetic relationships and population structure of garlic accessions.

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