Chungcheongnam do Agricultural Research and Extension Services

Yesan, South Korea

Chungcheongnam do Agricultural Research and Extension Services

Yesan, South Korea
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Byun H.-S.,Sungkyunkwan University | Kil E.-J.,Sungkyunkwan University | Seo H.,Sungkyunkwan University | Suh S.-S.,Korea Advanced Institute of Science and Technology | And 9 more authors.
Plant Disease | Year: 2016

Papaya (Carica papaya) is one of the most common tropical fruits throughout the world. In July 2014, symptoms of curling, deformation, and yellowing of young leaves, which are known typical symptoms of Papaya leaf curl virus (PaLCuV), were observed in papayas cultivated in greenhouses of Gokseong and Haenam located in the southern part of the Korean peninsula (Chang et al. 2003). Total DNA from six plants was extracted using a Viral Gene-spin viral DNA/RNA extraction kit (iNtRON Biotechnology, Seongnam, Korea) and used as templates in PCR amplifications with PaLCuV-F (5′-GCATTATGTATAGCCCACGA-3′) and PaLCuV-R (5′-GTCAGCCAATGATGCTTACG-3′) primers. The primer set was newly constructed based on the existing PaLCuV sequences reported in Taiwan (GenBank Accession Nos. JN703795 and KC161184). The amplicon of every reaction had the same predicted size, 250 bp. To obtain the full sequence of PaLCuV, sample DNA was amplified by rolling circle amplification and digested with the restriction enzyme BamHI, which cuts PaLCuV only once (Kil et al. 2014). The amplified DNA, of size 2.8 kb, was cloned into the pGEM-3zf(+) vector (Promega, Madison, WI) and sequenced by a commercial sequencing service (Macrogen Inc., Seoul, Korea). NCBI BLAST results revealed that PaLCuV isolate Korea (KT266873) has 99.5% sequence identity to PaLCuV isolate PF-1 (KC161184). A phylogenetic tree constructed with the full sequences of PaLCuV isolates also showed that PaLCuV isolate Korea has the closest relationship to the PF-1 isolate. Southern blot hybridization using the PCR product as a probe showed the typical double-stranded and single-stranded appearance of PaLCuV DNA. Interestingly, symptoms of PaLCuV appeared in spring, and the affected leaves did not grow and expand well until August. However, in September, newly produced leaves were asymptomatic. Dense scars caused by severe stunting persisted on the middle of trunks of infected plants. PCR analysis showed that the fully recovered leaves of PaLCuV-infected papaya contained PaLCuV DNA, but in lesser amounts than papaya leaves with severe symptoms. The phenomenon of recovery of symptoms from geminivirus infection has been reported, but to our knowledge, this is the first report of PaLCuV infecting papayas in Korea, and of recovery of its symptoms (Góngora-Castillo et al. 2012). As the average atmospheric temperature has risen, various tropical fruits have begun to be grown in Korea. But most seeds and seedlings of tropical crops cultivated in Korea have been imported from Southeast Asia, which is a repository of plant virus. Because of the risk from new and emerging viruses in imported materials and their unknown infectivity to new hosts in Korea, an effective quarantine system is important to manage these risks. © 2016, American Phytopathological Society. All rights reserved.


Kil E.-J.,Sungkyunkwan University | Seo H.,Sungkyunkwan University | Byun H.-S.,Sungkyunkwan University | Suh S.-S.,Korea Advanced Institute of Science and Technology | And 8 more authors.
Plant Disease | Year: 2016

Euphorbia leaf curl virus (EuLCV), belonging to the genus Begomovirus, has been identified in poinsettia (Euphorbia pulcherrima) and passion fruit (Passiflora edulis) plants cultivated in China and Taiwan (Cheng et al. 2014; Wu et al. 2011). In 2014, symptomatic passion fruit plants showing yellow spotting and curling of leaves were first identified from tropical crop farms of three different regions (Goheung, Haenam, and Damyang) located in the southern part of the Korean peninsula. Viral DNA of each sample (6 samples from 3 regions) was isolated (Viral Gene-spin Viral DNA/RNA Extraction Kit, iNtRON Biotech., Seongnam, Republic of Korea) and amplified through rolling circle amplification (TempliPhi Amplification Kit, GE Healthcare Life Sci., Uppsala, Sweden) following manufacturer’s instructions. Amplified DNA was digested by the restriction enzyme NheI (TaKaRa Bio, Shiga, Japan) and separated on an agarose gel. DNA fragments (2.8 kb) were purified from the gel, ligated into a pGEM-T easy vector (Promega, Madison, WI), and sequenced (Macrogen, Seoul, Republic of Korea). According to BLAST search results, the full-length genome sequence (GenBank Accession No. KT259282) of 2.8-kb DNA fragments showed 99.38% identity to the EuLCV Taiwan isolate (KC161185) and 98.47% identity to the Chinese (Shandong) isolate (KC852148). Polymerase chain reaction (PCR) using 2×Taq PCR MasterMix (Bioneer, Daejeon, Republic of Korea) with EuCV-specific primers designed based on the viral genome sequence of EuLCV Korea isolate in this study (EuLCV-F: 5′- CAGGATCGAGCCGTGCTTAT-3′, EuLCV-R: 5′-GCCCAATACCACGGGTAACA-3′, expected size: 225 bp) was conducted with samples from randomly collected symptomatic passion fruit plants. Most of the passion fruit plants (23/24) were identified as EuLCV-infected according to PCR. Southern blot hybridization using probe DNA amplified with EuLCV-F and EuLCV-R primers also supported the conclusion that EuLCV was infectious to passion fruit plants. This is the first identification of EuLCV in passion fruit plants from South Korea. In the same tropical crop farm in Haenam, papaya (Carica papaya) plants cultivated with EuLCV-infected passion fruits showed mild virus disease symptoms like mild yellow spotting and curling of leaves. Full-length sequence of the viral genome from papaya was also confirmed to be identical to that from passion fruit (100%). Results of PCR and Southern blot hybridization with symptomatic papaya leaves (10/10) indicated that all papaya plants cultivated with EuLCV-infected passion fruit plants were also infected with EuLCV. This is the first report of EuLCV infecting papaya plants. Recently, in some regions of South Korea, tropical crops using seedlings and seeds imported from Taiwan and other Southeast Asian nations were cultivated gradually due to global warming. Because viruses and other pathogens infecting tropical crops can pass across the border with newly introduced tropical plants and they can infect other plants as yet unconfirmed as hosts, import and cultivation of newly introduced crops should be done carefully. © 2016, American Phytopathological Society. All rights reserved.


PubMed | Biological Resources Research Center, Chungcheongnam do Agricultural Research and Extension Services, Sungkyunkwan University, Kyungpook National University and National Academy of Agricultural Science
Type: | Journal: Scientific reports | Year: 2016

Tomato yellow leaf curl virus (TYLCV) is one of the most well-known tomato-infecting begomoviruses and transmitted by Bemisia tabaci. Seed transmission has previously been reported for some RNA viruses, but TYLCV has not previously been described as a seed-borne virus. In 2013 and 2014, without whitefly-mediated transmission, TYLCV was detected in young tomato plants germinated from fallen fruits produced from TYLCV-infected tomato plants in the previous cultivation season. In addition, TYLCV-Israel (TYLCV-IL) was also detected in seeds and their seedlings of TYLCV-infected tomato plants that were infected by both viruliferous whitefly-mediated transmission and agro-inoculation. The seed infectivity was 20-100%, respectively, and the average transmission rate to seedlings was also 84.62% and 80.77%, respectively. TYLCV-tolerant tomatoes also produced TYLCV-infected seeds, but the amount of viral genome was less than seen in TYLCV-susceptible tomato plants. When tomato plants germinated from TYLCV-infected seeds, non-viruliferous whiteflies and healthy tomato plants were placed in an insect cage together, TYLCV was detected from whiteflies as well as receiver tomato plants six weeks later. Taken together, TYLCV-IL can be transmitted via seeds, and tomato plants germinated from TYLCV-infected seeds can be an inoculum source of TYLCV. This is the first report about TYLCV seed transmission in tomato.


Park S.-Y.,South Korean National Institute of Animal Science | Park W.T.,Chungnam National University | Park Y.C.,Chungcheongnam do Agricultural Research and Extension Services | Ju J.I.,Chungcheongnam do Agricultural Research and Extension Services | And 2 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2012

Lycium chinense has been used as a traditional medicine for centuries in Asia because of its positive effects on health. However, its functional components have not been elucidated. This study determines the levels of health-promoting lipophilic compounds, including carotenoids, tocopherols, and phytosterol, and those of 42 hydrophilic metabolites, including sugars, organic acids, alcohols, amines, and amino acids, in L. chinense fruit from 11 cultivars. The metabolite profiles were subjected to a principal component analysis (PCA), Pearson correlation analysis, and hierarchical clustering analysis (HCA). PCA showed the Cheongdang (LM-3) cultivar to be distinct from the others. The correlation results for a total of 55 compounds revealed strong correlations between the metabolites that participated on closely related pathways. The Cheongdang cultivar appears to be most suited for functional food production because of its high carotenoid, tocopherol, and phytosterol levels. These results indicate the usefulness of metabolite profiling as a tool for assessing the quality of food.


Song J.Y.,Chungnam National University | Seo M.W.,Chungnam National University | Kim S.I.,Chungcheongnam do Agricultural Research and Extension Services | Nam M.H.,Chungcheongnam do Agricultural Research and Extension Services | And 2 more authors.
Mycobiology | Year: 2014

We analyzed the genetic diversity of Cylindrocarpon destructans isolates obtained from Korean ginseng (i.e., Panax ginseng) roots by performing virulence tests and nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA sequence analysis. The phylogenetic relationship analysis performed using ITS DNA sequences and isolates from other hosts helped confirm that all the Korean C. destructans isolates belonged to Nectria/Neonectria radicicola complex. The results of in vivo and ex vivo virulence tests showed that the C. destructans isolates could be divided into two groups according to their distinctive difference in virulence and the genetic diversity. The highly virulent Korean isolates in pathogenicity group II (PG II), together with foreign isolates from P. ginseng and P. quinquefolius, formed a single group. The weakly virulent isolates in pathogenicity group I, together with the foreign isolates from other host plants, formed another group and exhibited a greater genetic diversity than the isolates of PG II, as confirmed by the mt SSU rDNA sequence analysis. In addition, as the weakly virulent Korean isolates were genetically very similar to the foreign isolates from other hosts, they were likely to originate from hosts other than the ginseng plants. © The Korean Society of Mycology.


Kim H.-K.,Chungcheongnam do Agricultural Research and Extension Services | Yang E.-S.,Chungcheongnam do Agricultural Research and Extension Services | Park G.-M.,Sungkyunkwan University | Kim G.-H.,Geumsan Ginseng and Medicinal Crop Experiment Station | And 2 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2011

This study was conducted to investigate the quality characteristics, antioxidant activity, and α-glucoamylase inhibitory activity of Dahyang, a Chungnam Agricultural Research and Extension Service's newly bred cultivar of brown button mushroom. Total phenolic compound contents of Dahyang and the no. 705 mushroom were 189±12 mg% and 168±8 mg%, respectively. The major free sugars in Dahyang were mannitol (3.11%), xylose (0.12%), and trehalose (0.08%). β-Glucan content was 28.34% in Dahyang and 26.55% in the no. 705 mushroom, respectively. Electron donating ability by DPPH in Dahyang and the no. 705 mushroom was 52.14% and 45.27% for the water extract, and 57.81% and 46.93% for the 80% ethanol extract, respectively. α-Glucoamylase inhibitory activity in a 10 mg/mL concentration of water extract were was 33.25% in Dahyang and 29.22% in the no. 705 mushroom, respectively.


Yoon M.-Y.,Kongju National University | Moe K.T.,Kongju National University | Kim D.-Y.,South Korean National Institute of Animal Science | Rho I.-R.,South Korean National Institute of Animal Science | And 5 more authors.
Electronic Journal of Biotechnology | Year: 2012

In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a single population in which > 70% of their inferred ancestry was derived from one of the model-based populations. However, two accessions (3.4%) in the sample were categorized as having admixed ancestry. Here, we report detailed information on commercially grown strawberry accessions from five different origins using SSR markers. These results couldbe used for broadening the genetic base of commercially grown varieties. © 2012 by Pontificia Universidad Católica de Valparaíso, Chile.


Kim D.C.,Chungcheongnam do Agricultural Research and Extension Services | Choi H.G.,Chungcheongnam do Agricultural Research and Extension Services | Pak H.S.,Chungcheongnam do Agricultural Research and Extension Services | Lee Y.H.,Chungcheongnam do Agricultural Research and Extension Services | And 3 more authors.
Korean Journal of Horticultural Science and Technology | Year: 2015

The new garden chrysanthemum (Dendranthema grandiflorum Ramat.) cultivar ‘Nuri Ball’ was developed at Yesan Chrysanthemum Experiment Station of Chungcheongnam-do Agricultural Research and Extension Services in 2011. ‘Nuri Ball’ was bred through a cross between the ‘02-145-01’ line as the female parent with yellow flower color and ‘02-04-32’ as the male plant with white flower color in 2004. Three years of adaptation trials were conducted from 2006 to 2009 under natural conditions. This study compared the external shape type with that of ‘White Miri’ and conducted ploidy and RAPD (Random amplified polymorphic DNA) marker analyses. These tests showed that ‘Nuri Ball’ cultivar has its own characteristics compared with the control ‘White Miri’. ‘Nuri Ball’ was a shrub type variety with semi-double flowers of 4.0 cm in width with white petals. It could produce 1025.2 flowers per plant in autumn. Compared with the control ‘White Miri’, ‘Nuri Ball’ was similar in terms of shape and color of flowers, but was different in flower size and number. The natural flowering time of ‘Nuri Ball’ was late September. It had very vigorous growth and an early budding plant. ‘Nuri Ball’ was demonstrated to be a new cultivar based on ploidy test and RAPD analysis. ‘Nuri Ball’ is intended for use as a bed chrysanthemum and expected to contribute to farm incomes in landscaping. © 2015 Korean Society for Horticultural Science.


Nam M.H.,Chungcheongnam do Agricultural Research and Extension Services | Kim H.S.,Chungcheongnam do Agricultural Research and Extension Services | Choi J.H.,Chungcheongnam do Agricultural Research and Extension Services | Lee H.D.,Chungcheongnam do Agricultural Research and Extension Services
Korean Journal of Horticultural Science and Technology | Year: 2013

Fungicide applications are required to prevent the strawberry from Botrytis fruit rot and powdery mildew that infect open strawberry flowers, however, their effects of fungicides on pollen germination of strawberry have been rarely documented, particularly those from recently developed active fungicidal ingredients. In this study we have evaluated the effects of 24 commercial fungicidal formulations and 6 organic materials on pollen germination in 3 strawberry cultivars using in vitro assays. Pollens from strawberry had higher germination rates on agar with sucrose of 18% and 25 °C than other tested conditions. Pollen germination rates of cvs. Seolhyang, Maehyang, and Kumhyang at 18% sucrose and 25 °C were 15.3, 18.4 and 30.7%, respectively. Pyraclostrobin, azoxystrobin, kresoxim-methyl, dichlofluanid, iminoctadine tris, and sulfur showed the strongest inhibitory efficacy with the germination rates of more than 93.8% compared to the no-fungicide control. Germination was not significantly affected by simeconazole and procymidone. This in vitro germination study may provide information useful for selecting fungicides in flowering stage to strawberry farmers. © 2013 Korean Society for Horticultural Science.


Jo M.H.,Chungcheongnam do Agricultural Research and Extension Services | Ham I.K.,Chungcheongnam do Agricultural Research and Extension Services | Moe K.T.,Kongju National University | Kwon S.-W.,Kongju National University | And 6 more authors.
Australian Journal of Crop Science | Year: 2012

Garlic bulb is an important seasoning ingredient in many of the world's cuisines. However, clonal lineages within this species show a remarkably high degree of phenotypic diversity in bulb size and color. Present study classified the genetic variations of garlic. Seven selected simple sequence repeats (SSRs) revealed a total of 37 alleles across 120 garlic accessions, with an average of seven alleles per locus. The values for observed heterozygosity ranged from 0 to 0.99 (mean = 0.71). The average genetic diversity and polymorphic information content values were 0.586 and 0.518, respectively. Based on the 37 alleles obtained from the seven SSRs, a phylogram was constructed to understand the relationships among the 120 accessions. Thegarlic accessions were clustered into fourmaingroups (G1-G4)in the phylogram. Group1 consisted ofaccessionsof 'Aomori', Group2 consisted of64 accessions, Group3 consisted of25 accessions, and Group4 consisted of20accessions. Our results indicate that genetic diversity is correlated with geographical region. There may have been local selection pressure and differences in adaptability of the garlic to different geographical conditions. All of the tested loci deviated significantly (P < 0.01) from Hardy-Weinberg equilibrium. Thus, a number of disturbances occurred in the garlic population tested, including natural selection. Our findings will helpexplain the genetic relationships and population structure of garlic accessions.

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