Chumphae Hospital

Chum Phae, Thailand

Chumphae Hospital

Chum Phae, Thailand
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Posuwan N.,Chulalongkorn University | Wanlapakorn N.,Chulalongkorn University | Sa-Nguanmoo P.,Chulalongkorn University | Wasitthankasem R.,Chulalongkorn University | And 15 more authors.
PLoS ONE | Year: 2016

Hepatitis B vaccination for newborns was introduced in two provinces in 1988 as part of Thailand's Expanded Program on Immunization (EPI), and extended to the whole country in 1992. Our previous studies showed that children and adolescents who were born after the implementation of this program had a carrier rate of less than 1%, compared with 5-6% before implementation. In 2014 we performed hepatitis B serosurveys among 5964 subjects in the different geographic regions of the country to evaluate the long-term immunogenicity and impact of universal hepatitis B vaccination in newborns as part of the 22-year EPI program, by assessing HBsAg, anti-HBc and anti-HBs seropositivity status. The number of HB virus (HBV) carriers, both children and young adults, who were born after universal HB vaccination was markedly reduced. The carrier rates among the age groups 6 months to 5 years, 5-10, 11-20, 21-30, 31-40, 41-50 and >50 years were respectively 0.1, 0.29, 0.69, 3.12, 3.78, 4.67 and 5.99%. The seropositivity rate for HBsAg in the post-EPI group was 0.6%, whereas in the pre-EPI group it was as high as 4.5% (p<0.001). HBV infection by means of detectable anti-HBc had also drastically declined in the population born after the HB vaccine was integrated into the EPI program. We estimated that the total number of HBV carriers amounted to 2.22 million, or 3.48%of the total population, most of whom are adults. The HB vaccine is the first vaccine shown to be effective in preventing the occurrence of chronic liver disease and hepatocellular carcinoma. Universal vaccination campaign will contribute to the eventual eradication of HBV-associated disease. © 2016 Posuwan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Chieochansin T.,Chulalongkorn University | Chieochansin T.,Mahidol University | Vutithanachot V.,Chumphae Hospital | Theamboonlers A.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University
Archives of Virology | Year: 2015

Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27 %) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear. © 2015, Springer-Verlag Wien.


Chieochansin T.,Chulalongkorn University | Chieochansin T.,Mahidol University | Vutithanachot V.,Chumphae Hospital | Phumpholsup T.,Chulalongkorn University | And 3 more authors.
Infection, Genetics and Evolution | Year: 2016

Human rotavirus A (RVA) is the major infectious virus causing acute watery diarrhea in children, especially those younger than 5 years of age, and is a major public health problem in Thailand. Outbreaks of this virus have been reported worldwide. Besides the common genotypes, unusual genotypes providing evidence of inter-species transmission have also been described. Therefore, the aim of this study was to investigate the prevalence and genotypes of RVA in Thailand. A total of 688 samples were collected from children who were hospitalized with acute diarrhea in Chumphae Hospital in Khon Kaen and Chulalongkorn Hospital in Bangkok. RVA was detected using one-step RT-PCR and the genotypes were evaluated by sequencing. Overall, 204 of the 688 samples (30%) were positive for RVA. Nine genotypes were identified: three common in humans (G1P[8] [53%], G2P[4] [18%], G3P[8] [12%]), one feline-like (G3P[9] [1%]), four porcine-like (G4P[6] [0.5%], G5P[6] [0.5%], G9P[8] [0.5%], G12P[6] [1.5%]), and one bovine-like (G8P[8] [13%]). The variation in virus genotypes and the animal-like genotypes detected in this study suggested that a high diversity of RVA types is circulating in the Thai population. Therefore, continuous molecular epidemiological monitoring of RVA is essential and has implications for the national vaccination program. © 2015 Elsevier B.V.


Prachayangprecha S.,Chulalongkorn University | Makkoch J.,Chulalongkorn University | Payungporn S.,Chulalongkorn University | Chieochansin T.,Chulalongkorn University | And 4 more authors.
Journal of Health, Population and Nutrition | Year: 2010

The study was aimed at determining the prevalence of pandemic influenza (H1N1) 2009 among patients with respiratory tract diseases during July-December 2009 using real-time reverse transcription polymerase chain reaction. Haemagglutination inhibition (HI) assay was performed to detect antibody titres against pandemic influenza in 255 medical personnel, 307 members of the general population during the second week of December 2009 in Khon Kaen province, Thailand, and in 100 stored sera collected from people of different age-groups during 2008. The results showed that the pandemic (H1N1) 2009 had occurred during July-December 2009. The results of the HI test after the wave of this outbreak showed that 123 (48%) of the 255 sera collected from the medical personnel, 109 (36%) of the 307 sera obtained from the general population, and only two of the 100 stored sera from 2008 contained antibodies (HI titres ≥40) against pandemic influenza. Antibody against the pandemic (H1N1) 2009 was found in at least one-third of the population. In conclusion, the prevalence of virus and serological data obtained from the study can be used as the serological background level of the Thai population after the July-December pandemic. Finally, the serological data might be useful for outbreak-prevention and control strategies and for the management of vaccination for the pandemic (H1N1) 2009 in Thailand. © International Centre for Diarrhoeal Disease Research, Bangladesh.


Khananurak K.,Chulalongkorn University | Vutithanachot V.,Chumphae Hospital | Simakachorn N.,Maharat Nakhon Ratchasima Hospital | Theamboonlers A.,Chulalongkorn University | And 2 more authors.
Infection, Genetics and Evolution | Year: 2010

Rotaviruses are the most common cause of severe diarrhea among infants and young children worldwide, especially in developing countries. In Thailand, rotavirus has presented a major public health problem causing severe diarrhea in infants and young children. It was responsible for about one-third of diarrheal diseases in hospitalized patients. In this study, we have analyzed the distribution and performed molecular characterization of rotaviruses circulating in infants and young children with diarrhea admitted to the city and rural hospitals in Thailand between July 2007 and May 2009. Group A human rotavirus was detected in 158 (28.4%) of 557 fecal specimens by RT-PCR. The peak incidence of infection was found in the winter months between December and March. The G1P[8] strain was identified as the most prevalent (49.4%) followed by G9P[8] (22.2%), G2P[4] (20.2%) and G3P[8] (0.6%). The uncommon strains G12P[8], G12P[6] and G3P[9] were also detected. Phylogenetic analysis of selected G and P genotypes isolated in this study was performed to compare with the reference strains from different countries. Emergence of G12 in the northern part of Thailand was observed and phylogenetic analysis demonstrated close relation between Thai isolates and strains from India. The present study reveals the recurring changing genotypes of rotavirus circulating in Thailand. The genetic association between isolates from Thailand and other countries ought to be considered with regard to local and global dissemination of rotavirus as it is crucial for prevention especially, with respect to vaccine implementation. © 2010 Elsevier B.V.


Sa-Nguanmoo P.,Chulalongkorn University | Posuwan N.,Chulalongkorn University | Vichaiwattana P.,Chulalongkorn University | Vuthitanachot V.,Chumphae Hospital | And 12 more authors.
PLoS ONE | Year: 2016

Hepatitis A virus (HAV) is transmitted via the fecal-oral route from contaminated food or water. As part of the most recent survey of viral hepatitis burden in Thailand, we analyzed the current seroprevalence of HAV in the country and compared with data dating back to 1971. From March to October, 2014, a total of 4,260 individuals between one month and 71 years of age from different geographical regions (North = 961; Central = 1,125; Northeast = 1,109; South = 1,065) were screened for anti-HAV IgG antibody using an automated chemiluminescent microparticle immunoassay. Overall, 34.53% (1,471/4,260) possessed anti-HAV IgG antibody, and the age-standardized seroprevalence was 48.6%. Seroprevalence rates were 27.3% (North), 30.8% (Central), 33.8% (Northeast) and 45.8% (South) and were markedly lower than in the past studies especially among younger age groups. The overall trend showed an increase in the age by which 50% of the population were anti-HAV IgG antibody: 4.48 years (1971-1972), 6(1976), 12.49(1990), 36.02 (2004) and 42.03 (2014). This suggests that Thailand is transitioning from low to very low HAV endemicity. Lower prevalence of HAV correlated with improved healthcare system as measured by decreased infant mortality rate and improved national economy based on increased GDP per capita. The aging HAV immuno-naïve population may be rendered susceptible to potential HAV outbreaks similar to those in industrialized countries and may benefit from targeted vaccination of high-risk groups. © 2016 Sa-nguanmoo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Chirathaworn C.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University | Vuthitanachot V.,Chumphae Hospital
Asian Pacific Journal of Tropical Medicine | Year: 2010

Objective: To determine the levels of IL-18 and IL-18 binding protein (BP) in patients with dengue virus infection. Methods: Acute and convalescent sera were collected from each patient. Control group was sera from blood donors. The levels of both IL-18 and IL-18BP were measured by ELISA assays. Results: It was shown that IL-18 and IL-18BP levels were significantly higher in patients when compared with controls. In addition, the level of IL-18BP was lower in convalescent than in acute sera. Conclusions: These data suggest that both IL-18 and IL-18BP production was induced following dengue virus infection. Investigating the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection. © 2010 Hainan Medical College.


Chieochansin T.,Chulalongkorn University | Vutithanachot V.,Chumphae Hospital | Theamboonlers A.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University
Clinical Laboratory | Year: 2014

Background: Rapid tests are widely used to detect rotavirus A; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. Therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step RT-PCR method. Methods: Seven hundred fifty-five stool samples were collected from children with acute diarrhea. All samples were processed immediately after arrival with the SD BIOLINE rota rapid test and one-step RT-PCR method. Results: The detection rates of rotavirus A were 40.79% and 41.91% for the rapid test and one-step RT-PCR, respectively. The rapid test had 93.57% sensitivity and 96.17% specificity. Most of the different genotypes of rotavirus A were detected with the SD rapid test. Conclusions: Although the rapid test is able to quickly give results, we found that it has high false positive and negative rates. Thus, other highly sensitive methods such as one-step RT-PCR are still required for true diagnosis.


Prachayangprecha S.,Chulalongkorn University | Makkoch J.,Chulalongkorn University | Vuthitanachot C.,Chulalongkorn University | Vuthitanachot V.,Chulalongkorn University | And 4 more authors.
Japanese Journal of Infectious Diseases | Year: 2011

Since April 2009, the outbreak of human pandemic influenza A (H1N1) virus (pH1N1) infection has spread from North America to other parts of the world, and currently, pH1N1 is the predominant circulating strain of influenza viruses. Our objectives were to perform a serological survey of medical personnel at the Chumphae Hospital in Thailand and to investigate the prevalence of pH1N1 in randomly selected patients diagnosed with respiratory tract disease. Prevalence of pH1N1 in the patients was determined by performing real-time reverse transcription-polymerase chain reaction. The study was carried out between July 2009 and November 2010. Seroprevalence of hemaglutination inhibition (HI) titers among medical personnel was established in three cross-sectional studies at the end of each wave of the pandemic by performing HI assay to detect antibodies against pH1N1. Infection by the pH1N1 peaked between July and October 2009; the second wave was from January to March 2010 and the third wave from June to November 2010. The HI titers after the first, second, and third waves were 48.2z, 22.4z, and 25.7z, respectively. After the second and third waves, 52.1z and 45.3z of the medical personnel who had received pH1N1 vaccination had HI titers ≥40. These findings show that seasonal influenza strain in Chumphae and the predominant influenza strain from each wave was pH1N1. HI assay results also represent the severity of the attack rate in each wave.


PubMed | Chulalongkorn University, Mahidol University and Chumphae Hospital
Type: | Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Year: 2015

Human rotavirus A (RVA) is the major infectious virus causing acute watery diarrhea in children, especially those younger than 5 years of age, and is a major public health problem in Thailand. Outbreaks of this virus have been reported worldwide. Besides the common genotypes, unusual genotypes providing evidence of inter-species transmission have also been described. Therefore, the aim of this study was to investigate the prevalence and genotypes of RVA in Thailand. A total of 688 samples were collected from children who were hospitalized with acute diarrhea in Chumphae Hospital in Khon Kaen and Chulalongkorn Hospital in Bangkok. RVA was detected using one-step RT-PCR and the genotypes were evaluated by sequencing. Overall, 204 of the 688 samples (30%) were positive for RVA. Nine genotypes were identified: three common in humans (G1P[8] [53%], G2P[4] [18%], G3P[8] [12%]), one feline-like (G3P[9] [1%]), four porcine-like (G4P[6] [0.5%], G5P[6] [0.5%], G9P[8] [0.5%], G12P[6] [1.5%]), and one bovine-like (G8P[8] [13%]). The variation in virus genotypes and the animal-like genotypes detected in this study suggested that a high diversity of RVA types is circulating in the Thai population. Therefore, continuous molecular epidemiological monitoring of RVA is essential and has implications for the national vaccination program.

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