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Chum Phae, Thailand

Chieochansin T.,Chulalongkorn University | Chieochansin T.,Mahidol University | Vutithanachot V.,Chumphae Hospital | Theamboonlers A.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University
Archives of Virology | Year: 2015

Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27 %) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear. © 2015, Springer-Verlag Wien.

Khananurak K.,Chulalongkorn University | Vutithanachot V.,Chumphae Hospital | Simakachorn N.,Maharat Nakhon Ratchasima Hospital | Theamboonlers A.,Chulalongkorn University | And 2 more authors.
Infection, Genetics and Evolution | Year: 2010

Rotaviruses are the most common cause of severe diarrhea among infants and young children worldwide, especially in developing countries. In Thailand, rotavirus has presented a major public health problem causing severe diarrhea in infants and young children. It was responsible for about one-third of diarrheal diseases in hospitalized patients. In this study, we have analyzed the distribution and performed molecular characterization of rotaviruses circulating in infants and young children with diarrhea admitted to the city and rural hospitals in Thailand between July 2007 and May 2009. Group A human rotavirus was detected in 158 (28.4%) of 557 fecal specimens by RT-PCR. The peak incidence of infection was found in the winter months between December and March. The G1P[8] strain was identified as the most prevalent (49.4%) followed by G9P[8] (22.2%), G2P[4] (20.2%) and G3P[8] (0.6%). The uncommon strains G12P[8], G12P[6] and G3P[9] were also detected. Phylogenetic analysis of selected G and P genotypes isolated in this study was performed to compare with the reference strains from different countries. Emergence of G12 in the northern part of Thailand was observed and phylogenetic analysis demonstrated close relation between Thai isolates and strains from India. The present study reveals the recurring changing genotypes of rotavirus circulating in Thailand. The genetic association between isolates from Thailand and other countries ought to be considered with regard to local and global dissemination of rotavirus as it is crucial for prevention especially, with respect to vaccine implementation. © 2010 Elsevier B.V.

Chirathaworn C.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University | Vuthitanachot V.,Chumphae Hospital
Asian Pacific Journal of Tropical Medicine | Year: 2010

Objective: To determine the levels of IL-18 and IL-18 binding protein (BP) in patients with dengue virus infection. Methods: Acute and convalescent sera were collected from each patient. Control group was sera from blood donors. The levels of both IL-18 and IL-18BP were measured by ELISA assays. Results: It was shown that IL-18 and IL-18BP levels were significantly higher in patients when compared with controls. In addition, the level of IL-18BP was lower in convalescent than in acute sera. Conclusions: These data suggest that both IL-18 and IL-18BP production was induced following dengue virus infection. Investigating the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection. © 2010 Hainan Medical College.

Chieochansin T.,Chulalongkorn University | Vutithanachot V.,Chumphae Hospital | Theamboonlers A.,Chulalongkorn University | Poovorawan Y.,Chulalongkorn University
Clinical Laboratory | Year: 2014

Background: Rapid tests are widely used to detect rotavirus A; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. Therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step RT-PCR method. Methods: Seven hundred fifty-five stool samples were collected from children with acute diarrhea. All samples were processed immediately after arrival with the SD BIOLINE rota rapid test and one-step RT-PCR method. Results: The detection rates of rotavirus A were 40.79% and 41.91% for the rapid test and one-step RT-PCR, respectively. The rapid test had 93.57% sensitivity and 96.17% specificity. Most of the different genotypes of rotavirus A were detected with the SD rapid test. Conclusions: Although the rapid test is able to quickly give results, we found that it has high false positive and negative rates. Thus, other highly sensitive methods such as one-step RT-PCR are still required for true diagnosis.

Prachayangprecha S.,Chulalongkorn University | Makkoch J.,Chulalongkorn University | Vuthitanachot C.,Chulalongkorn University | Vuthitanachot V.,Chulalongkorn University | And 4 more authors.
Japanese Journal of Infectious Diseases | Year: 2011

Since April 2009, the outbreak of human pandemic influenza A (H1N1) virus (pH1N1) infection has spread from North America to other parts of the world, and currently, pH1N1 is the predominant circulating strain of influenza viruses. Our objectives were to perform a serological survey of medical personnel at the Chumphae Hospital in Thailand and to investigate the prevalence of pH1N1 in randomly selected patients diagnosed with respiratory tract disease. Prevalence of pH1N1 in the patients was determined by performing real-time reverse transcription-polymerase chain reaction. The study was carried out between July 2009 and November 2010. Seroprevalence of hemaglutination inhibition (HI) titers among medical personnel was established in three cross-sectional studies at the end of each wave of the pandemic by performing HI assay to detect antibodies against pH1N1. Infection by the pH1N1 peaked between July and October 2009; the second wave was from January to March 2010 and the third wave from June to November 2010. The HI titers after the first, second, and third waves were 48.2z, 22.4z, and 25.7z, respectively. After the second and third waves, 52.1z and 45.3z of the medical personnel who had received pH1N1 vaccination had HI titers ≥40. These findings show that seasonal influenza strain in Chumphae and the predominant influenza strain from each wave was pH1N1. HI assay results also represent the severity of the attack rate in each wave.

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