Liu X.-G.,Chui Yang liu Hospital of Beijing |
Deng Y.-B.,Sun Yat Sen University |
Cai H.,Chui Yang liu Hospital of Beijing |
Wang L.-N.,Chui Yang liu Hospital of Beijing |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Previous studies have demonstrated that transplantation of controlled release glial cell line-derived neurotrophic factor (GDNF) and bone marrow mesenchymal stem cells-derived neuron-like cells (BMSCs) can effectively promote the motor function recovery of rhesus monkeys with spinal cord injury. Objective: To validate whether GDNF+BMSCs transplantation exhibits better protective effects on spinal cord tissue of rhesus monkeys with spinal cord injury than BMSCs transplantation. Methods: Rhesus monkey models of acute severe spinal cord injury were divided into three groups and received GDNF+BMSCs transplantation (GDNF+BMSCs group) or BMSCs transplantation (BMSCs group) or PBS administration (control group). Spinal cord tissue was embedded with paraffin and stained with hematoxylin-eosin. Myelosyringosis was estimated by image analysis system. Results: In the control group, spinal cord structure was greatly destructed, and myelosyringosis occupied larger area. In the BMSCs group, spinal cord structure was well preserved, and small cavities, occasionally large cavities, were observed. In the GDNF+BMSCs group, spinal cord structure was best preserved and only small cavities were observed. There was significant difference in spinal cord structure destruction between groups (P < 0.01). These findings suggest that compared with simple BMSCs transplantation, transplantation of controlled released GDNF+BMSCs shows better protective effects on spinal tissue structure after spinal cord injury, which may be one of mechanisms by which myelosyringosis is reduced to a greater extent.