Hiroshima-shi, Japan
Hiroshima-shi, Japan

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Ise K.,Japan Atomic Energy Agency | Sasaki Y.,Japan Atomic Energy Agency | Amano Y.,Japan Atomic Energy Agency | Iwatsuki T.,Japan Atomic Energy Agency | And 3 more authors.
Geomicrobiology Journal | Year: 2017

We investigated the change in bacterial community structure after drilling boreholes, 09-V250-M02 and 09-V250-M03, in the 250-m deep research gallery of the Horonobe Underground Research Laboratory. In the 09-V250-M02 borehole, ϵ-Proteobacteria were predominantly detected in the clone library analyses of the groundwater samples conducted immediately after drilling. All the ϵ-Proteobacteria clones were closely related to Arcobacter spp., which are known to be sulfide-oxidizing chemoautotrophic bacteria. After 4 years, the microbial structure drastically changed, and most detected operational taxonomic units were uncultured species such as candidate division OP9 and Chloroflexi relatives, which are frequently detected in deep sea sediments. The results indicated that the microbial community structure was drastically affected by borehole drilling and was concomitant with oxidation perturbation. However, these disturbed microbial communities changed within a few years to a microbial community composed of uncultivated species such as OP9 and Chloroflexi. 2016 © 2017 Taylor & Francis Group, LLC

Suzuki T.,Shibaura Institute of Technology | Nakamura T.,Chugai Technos Corporation | Fuse H.,Shibaura Institute of Technology
Microbes and Environments | Year: 2011

Two novel ethylene-assimilating bacteria, strains ETY-M and ETY-NAG, were isolated from seawater around Japan. The characteristics of both strains were investigated, and phylogenetic analyses of their 16S rRNA gene sequences showed that they belonged to the genus Haliea. In C1-4 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in addition to ethylene. Methane, n-butane, and i-butane were not utilized or degraded by either strain. Soluble methane monooxygenase-type genes, which are ubiquitous in alkene-assimilating bacteria for initial oxidation of alkenes, were not detected in these strains, although genes similar to particulate methane monooxygenases (pMMO)/ammonia monooxygenases (AMO) were observed. The phylogenetic tree of the deduced amino acid sequences formed a new clade near the monooxygenases of ethane-assimilating bacteria similar to other clades of pMMOs in type I, type II, and Verrucomicrobia methanotrophs and AMOs in alpha and beta proteobacteria.

Hirayama H.,Japan Agency for Marine - Earth Science and Technology | Fuse H.,Shibaura Institute of Technology | Abe M.,Japan Agency for Marine - Earth Science and Technology | Miyazaki M.,Japan Agency for Marine - Earth Science and Technology | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4 T and T2-1 were Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum and type I intracytoplasmic membranes. They were obligate methanotrophs that grew only on methane or methanol. Each strain possessed the particulate methane monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. The strains grew best at 37 °C, and did not grow at 45 °C. NaCl was required for growth within a concentration range of 1-8 % (w/v). The major phospholipid fatty acids were C 16: 0, C 16: 1ω7c, and C 16: 1ω5t. The major isoprenoid quinone was MQ-8. The DNA G+C content was 50.9-51.7 mol%. The 16S rRNA gene sequences of the strains showed 99.4 % similarity to each other, and DNA-DNA hybridization analysis indicated that the strains were representatives of the same species. The 16S rRNA gene sequences were highly similar to some marine environmental sequences (94.0-97.7 % similarity), but did not show similarities more than 94 % with sequences of members of other related genera, such as Methylomicrobium, Methylobacter, Methylomonas and Methylosarcina. Phylogenies based on 16S rRNA gene sequences and deduced partial PmoA sequences, and the physiological and chemotaxonomic characteristics revealed that strains IT-4 T and T2-1 represent a novel species of a new genus in the family Methylococcaceae, for which the name Methylomarinum vadi gen. nov., sp. nov. is proposed. The type strain is IT-4 T (= JCM 13665 T = DSM 18976 T). © 2013 IUMS.

Yamauchi T.,Toyama Institute of Health | Yoshigou H.,Chugai Technos Co. | Itoh T.,Marine Biological Research Institute of Japan Co.
Comparative Parasitology | Year: 2013

ABSTRACT: We present the first record of Parabdella quadrioculata (Annelida: Hirudinida: Glossiphoniidae) from Japan. Leeches were collected from the Japanese pond turtle Mauremys japonica and a Japanese man. Mauremys japonica is a new host for this leech. This report is the first case of human infestation by the glossiphoniid leech.

PubMed | Ibaraki University, Meiji University and Chugai Technos Corporation
Type: Journal Article | Journal: International journal of phytoremediation | Year: 2016

Several species of ornamental flowering plants were evaluated regarding their phytoremediation ability for the cleanup of oil-contaminated soil in Japanese environmental conditions. Thirty-three species of plants were grown in oil-contaminated soil, and Mimosa, Zinnia, Gazania, and cypress vine were selected for further assessment on the basis of their favorable initial growth. No significant difference was observed in the above-ground and under-ground dry matter weight of Gazania 180 days after sowing between contaminated and non-contaminated plots. However, the other 3 species of plants died by the 180th day, indicating that Gazania has an especially strong tolerance for oil-contaminated soil. The total petroleum hydrocarbon concentration of the soils in which the 4 species of plants were grown decreased by 45-49% by the 180th day. Compared to an irrigated plot, the dehydrogenase activity of the contaminated soil also increased significantly, indicating a phytoremediation effect by the 4 tested plants. Mimosa, Zinnia, and cypress vine all died by the 180th day after seeding, but the roots themselves became a source of nutrients for the soil microorganisms, which led to a phytoremediation effect by increase in the oil degradation activity. It has been indicated that Gazania is most appropriate for phytoremediation of oil-contaminated soil.

Yamane K.,Japan National Food Research Institute | Hattori Y.,Chugai Technos Co. | Ohtagaki H.,Chugai Technos Co. | Fujiwara K.,Chugai Technos Co.
FEMS Microbiology Ecology | Year: 2011

We prepared DNA from the production waters of oil deposits and wellheads of the high- and hypertemperature Japanese oil wells #AR39 (depth, 1230m; temperature, 74°C; pressure, 2.92MPa) and #SR123 (depth, 1687m; temperature, 98°C; pressure, 11.3MPa) to detect indigenous bacterial and archaeal microorganisms. We used PCR to amplify the 16S rRNA genes of microbial communities and characterized them based on their sequences. A few species of microorganisms with high GC contents were detected in samples from oil deposits, whereas the microbial constituents and their GC contents were diverse in wellhead samples. A comparison of the composition of the microbial communities found that the predominant indigenous populations in the #SR123 oil deposit were Thermotoga hypogea-, Thermotoga petrophila- and Thermodesulfobacterium commune-like bacteria with a 61-63% GC content in their 16S rRNA gene sequences, and Archaeoglobus fulgidus-like archaea with a 65% GC content, whereas the major population in #AR39 comprised Thermacetogenium phaeum- and Fervidobacterium pennavorans-like bacteria and Methanothermobacter thermautotrophicus-like archaea with a 60%, 60% and 61% GC content, respectively. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.

Lee Y.-J.,Korea University | Lee Y.-J.,Korea Institute of Energy Research | Kawamura T.,Japan National Institute of Advanced Industrial Science and Technology | Kawamura T.,Chugai Technos Corporation | And 2 more authors.
Journal of Chemical and Engineering Data | Year: 2012

The hydrate phase equilibrium behaviors of tetrahydrofuran (THF) + CH 4, THF + CO2, CH4 + CO2, and THF + CO2 + CH4 were investigated over wide ranges of temperature, pressure, and concentration. The dissociation conditions of THF + CH4 and THF + CO2 hydrates were shifted to lower pressures and higher temperatures from the dissociation boundaries of pure CH4 and pure CO2 hydrates. X-ray diffraction results revealed that the CH4 + CO2 and THF + CO2 + CH4 hydrates prepared from a CH4/CO2 (50:50) gas mixture formed structure I and II clathrate hydrates, respectively. Raman measurements provided detailed information regarding the cage occupancy of CH4 and CO2 molecules encaged in the hydrate frameworks. For the CH 4 + CO2 hydrates, the concentrations of CO2 in the hydrate phase were higher than those in the vapor phase. In contrast, for the THF + CO2 + CH4 hydrates, the concentrations of CO2 in the hydrate phase were lower than those in the vapor phase. © 2012 American Chemical Society.

Noriyuki M.,Chugai Technos Corporation | Okuda S.,Nakaochiai
Journal of Japanese Botany | Year: 2014

A saprophytic plant, Sciaphila nana Blume (Triuridaceae), ranging from Honshu, Shikoku, Kyushu to Okinawa Island, Japan (Ohashi et al. 2008), was collected in the mainland part of Tokyo and Tochigj Pref, Honshu, Japan, in 2007 and 2013. All of these had female flowers. This is the first record for the mainland part of Tokyo Pref. In Tochigi Pref., this is a rediscovery after 70 years from the first finding. Both habitats were floors of abandoned, secondary forests of Quercus serrata. Owing to the cessation of ordinary maintenance, the habitats might be kept in appropriate conditions for Sciaphila nana. A saprophytic orchid, Lecanorchis nigricans Honda, was also found in both localities.

Saito H.,Tokai University | Mochizuki A.,Chugai Technos Corporation | Yano K.,Tokai University
Japanese Journal of Limnology | Year: 2016

In oligotrophic lakes, littoral benthic communities are diverse with due to multiple supply routes of production. In the present study, the spatial and temporal patterns of benthic assemblages and their potential food resources were investigated for three habitat types (cobble, pebble and sand) in the littoral zone of a large oligotrophic lake, Lake Shikotsu of northern Japan, from June 2006 to June 2007. A total of 22,112 observations representing 47 taxa were recorded. Spatial and temporal patterns of taxa richness, number of individuals and biomass of benthic assemblages revealed high variation among habitat types and across sampling periods. On a spatial scale, taxa richness, number of individuals and biomass of benthic assemblages in the cobble habitat showed the highest values among the three habitat types. Three potential food resources (chlorophyll a of algae, coarse particulate organic matter and fine particulate organic matter) also indicated high variation among sampling sites and sampling periods, with the most abundant resources found in the cobble habitat. Among the four functional feeding groups of benthic assemblages examined, collector-gatherers (49–81%) represented the largest number of individuals (herbivores, 20–35%, predators, 11–15%, and shredders, 1–5%). Dominant taxa collected in this study consisted of two mayflies (Paraleptophlebia sp. and Caenis sp.) and four midges (Orthocladiinae, Tanypodinae, Diamesinae and Chironominae). Five out of the six dominant taxa belonged to collector-gatherers, with the exception of Tanypodinae. Despite such high value of collector-gatherers in benthic assemblages, multiple regression analysis indicated that chlorophyll a and coarse particulate organic matter were better predictor elements in potential food resources for benthic assemblages than fine particulate organic matters. Our results suggest that spatial and temporal patterns of benthic assemblages in littoral zones of oligotrophic lakes exhibit high variation in abundance, reflecting the complex relationship with substrate type, canopy opening and resource abundance in each habitat. © 2016, Japanese Society of Limnology. All rights reserved.

Chugai Technos Corporation | Date: 2014-03-20

Provided is a carbon dioxide ground leakage monitoring system that is low cost, capable of real-time multi-point monitoring on a wide scale, and capable of providing the public with easy-to-understand information. A plurality of monitoring posts 100 and a field data server 200 and the field data server 200 and an administrative server 300 are connected by communication lines in respective sections therebetween. The plurality of monitoring posts 100 measure a concentration of carbon dioxide having leaked above the ground, and transmits the measured carbon dioxide data to the field data server 200, the field data server 200 transmits the data transmitted from the plurality of monitoring posts 100 to the administrative server 300, and the administrative server 300 discloses carbon dioxide ground leakage information on the Internet based on the carbon dioxide data transmitted from the field data server 200.

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