Chongqing Tobacco Science Research Institute

Chongqing, China

Chongqing Tobacco Science Research Institute

Chongqing, China
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Li P.,Southwest University | Ding W.,Southwest University | Ding W.,Chongqing Tobacco Science Research Institute | Liu Q.,Southwest University | And 5 more authors.
Tobacco Science and Technology | Year: 2016

To evaluate the effects of foliar spraying of two inducers (mineral elemental silicon, Si; plant elicitor benzothiadiazole, BTH) on tobacco bacterial wilt, the content of tobacco bacteria wilt pathogen in tobacco roots, the Si contents in tobacco roots, stalks and leaves, the activity of defense enzymes and the expressions of resistance genes in tobacco leaves and the incidence of tobacco bacteria wilt in laboratory and fields were analyzed after Si and BTH treatments. The results showed that the content of tobacco bacteria wilt pathogen in roots was significantly reduced by Si and BTH, and the Si content in the roots was significantly increased by the Si treatment. The activities of defense enzymes in leaves were also enhanced by the Si and BTH treatments, in which the activities of peroxidase (POD) and β-1, 3-glucanase (GLU) were significantly promoted by the Si treatment, as did those of GLU, polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL) by BTH. The expressions of resistance genes (EFE26, ACC Oxidase, HIN1 and PR2) in tobacco were significantly increased by both procedures, the expressions of PR1, PR1a/c, EFE26, ACC Oxidase and HIN1 were significantly higher in tobacco treated with BTH; while the expression of PR2 was significantly higher in tobacco treated with the Si treatment. Si induced the resistance to tobacco bacterial wilt through enhancing the Si accumulation in tobacco roots, as did BTH through promoting the expressions of resistance-related genes. In summary, this study demonstrated that foliar application of Si and BTH induced the resistance to tobacco bacterial wilt in tobacco in two different mechanisms. © 2016, Editorial Office of Tobacco Science & Technology. All right reserved.


Li S.,Southwest University | Liu Y.,Southwest University | Wang J.,Southwest University | Yang L.,Southwest University | And 3 more authors.
Frontiers in Microbiology | Year: 2017

Soil acidification is a major problem in modern agricultural systems and is an important factor affecting the soil microbial community and soil health. However, little is known about the effect of soil acidification on soil-borne plant diseases. We performed a 4-year investigation in South China to evaluate the correlation between soil acidification and the occurrence of bacterial wilt. The results showed that the average soil pH in fields infected by bacterial wilt disease was much lower than that in non-disease fields. Moreover, the proportion of infected soils with pH lower than 5.5 was much higher than that of non-infected soils, and this phenomenon became more obvious as the area of bacterial wilt disease increased at soil pH lower than 5.5 from 2011 to 2014. Then, in a field pot experiment, bacterial wilt disease developed more quickly and severely in acidic conditions of pH 4.5, 5.0, and 5.5. These results indicate that soil acidification can cause the outbreak of bacterial wilt disease. Further experiments showed that acidic conditions (pH 4.5-5.5) favored the growth of the pathogen Ralstonia solanacearum but suppressed the growth and antagonistic activity of antagonistic bacteria of Pseudomonas fluorescens and Bacillus cereus. Moreover, acidic conditions of pH 5.5 were conducive to the expression of the virulence genes PopA, PrhA, and SolR but restrained resistance gene expression in tobacco. Finally, application of wood ash and lime as soil pH amendments improved soil pH and reduced the occurrence of bacterial wilt. Together, these findings improve our understanding of the correlation between soil acidification and soil-borne plant diseases and also suggest that regulation of soil acidification is the precondition and foundation of controlling bacterial wilt. © 2017 Li, Liu, Wang, Yang, Zhang, Xu and Ding.


Zhao M.,Chongqing Tobacco Science Research Institute | Xu C.,Chongqing Tobacco Science Research Institute | Jia L.,State Key Laboratory of Silkworm Genome Biology | Cheng T.,State Key Laboratory of Silkworm Genome Biology | And 3 more authors.
Acta Tabacaria Sinica | Year: 2016

To rapidly and accurately detect bacterial population on tobacco leaves, gene chip was used for screening bacterial species specific probes from metagenomic sequencing data of varieties grown in main tobacco grown areas. Results showed that the overall quality of sequencing reads was relatively high, with percentage of Q20 and Q30 of 99.2% and 81.4%, respectively. The sequencing length was 100 bp and with well-distributed base. 200 specific candidate probes were identified from blast comparison results, which belong to 9 types of bacteria genera, and 165 probes belong to Pseudomonas. The results showed that gene chip technology can be applied to detect bacteria population in tobacco leaves. © 2016, Editorial Office Chinese Tobacco Society. All right reserved.


He M.-J.,Hunan Agricultural University | He M.-J.,China Agricultural University | Fan T.-F.,China Agricultural University | Yang C.,Chongqing Tobacco Science Research Institute | And 5 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2017

In this article, by using a nutrient-hydroponic culture method, 15N-substrate labeling and mass spectrum-based element measurement techniques, we identified firstly a minimum time when N was not transported to upper parts of two tobacco (Nicotiana tabacum) varieties after their root uptake of NH4 + and NO3 -, and subsequently characterized processes and components of N absorption by roots. Our data show that the time for the movement of N from its uptake by the roots to the upper parts of the two varieties happened within only several minutes, and that at least two different systems for the N uptake should exist in the tobacco roots. When external N concentrations were ≤1 mmol·L-1, the process of the root N-uptake displayed an enzymatic action property; KM values [i.e. 'K326':84.5 μmol·L-1; 'Honghuadajinyuan' ('HD'): 47.8 μmol·L-1] for NO3 - absorbed by the roots were significantly lower than that for NH4 + ('K326':93.5 μmol·L-1; 'HD':90.4 μmol·L-1), suggesting that the lower KM for NH4 + or NO3 - uptake by 'HD' would be one of critical physiological factors for a better tolerance of 'HD' to low N than that of 'K326'. As external N>1 mmol·L-1, N acquisition by the roots was positively proportional to external N concentrations; the rate of NO3 - uptake by 'K326' was more than 2-fold higher than that of by 'HD', and the rate of NH4 + uptake by 'K326' was 2.4-fold lower than that of by 'HD'; the uptake rate of 'K326' for NO3 - was 3.6-fold higher than for NH4 +, and 'HD' for NO3 - was half as many as for NH4 +. Our results may provide physiological evidence for further exploration of molecular mechanisms of N uptake by the roots of tobacco plants. © 2017, Science Press. All right reserved.


Jiang H.,Chongqing Tobacco Science Research Institute | Jiang H.,Henan Agricultural University | Chen T.,Chongqing Tobacco Company | Ma H.,Chongqing Tobacco Company | And 3 more authors.
Tobacco Science and Technology | Year: 2014

In order to find out the main factors influencing the sensory quality of flue-cured tobacco in Chongqing tobacco growing areas, the relations of appearance quality, physical properties, chemical components, and the harmony between those components with the sensory quality of tobacco were investigated with canonical correlation analysis. The results showed that the total sugar content in tobacco leaves was moderate or higher, the ratio of sugar to nicotine was slightly higher; the contents of reducing sugar, nicotine, total nitrogen and the ratios of nitrogen to nicotine and potassium to chloride were appropriate; while the contents of potassium and chloride were lower. The aromatic quality of tobacco leaves mainly affected by and positively correlated to total sugar, the difference between total sugar and reducing sugar, the ratio of nitrogen to nicotine, leaf color and body. The smoke concentration and impact mainly affected by and positively correlated to nicotine, total nitrogen, weight per unit leaf area, leaf oil and body, while affected by and significantly negatively correlated to potassium and the ratios of sugar to nicotine and potassium to chloride. Offensive odor mainly affected by and positively correlated to total sugar, the ratio of nitrogen to nicotine and the difference between total sugar and reducing sugar. Smoke sweetness mainly affected by and positively correlated to the ratio of nitrogen to nicotine and the difference between total sugar and reducing sugar.


Jiang H.,Chongqing Tobacco Science Research Institute | Li N.,Chongqing Tobacco Science Research Institute | Shan P.,Dali Branch of Yunnan Province Tobacco Company | Wang H.,Chongqing Tobacco Science Research Institute | And 2 more authors.
Tobacco Science and Technology | Year: 2013

For producing flue-cured tobacco seedlings at the areas of low temperature and less sunshine, closed seedling producing technique was studied with a self-configured closed seedling production system for flue-cured tobacco, the working principle of the system was introduced and its specifications and parameters were determined. The results of experiments showed that it only needed 22 days for cultivating seedlings of 4.5 leaf age with this technique, which was 24 days shorter than that with a greenhouse floating seedling system; the stalk circumference of seedling, the dry weight of leaf, stalk and root, root-shoot ratio, and vigorous seedling index increased by 35.29%, 8.48%, 49.23%, 160.00%, 74.22% and 83.78%, respectively; moreover, the costs of direct and heeled-in transplanting of the seedlings from closed system reduced by 101.3 and 30.4 RMB yuan per 667 m2, respectively. Closed seedling producing technique effectively solved the problems of longer seedling age and lower vigorous seedling ratio at areas of low temperature and less sunshine, it reduced the consumption of non-renewable peat resource and more significantly the cost of seedling production.


Li S.,Southwest University | Xu C.,Chongqing Tobacco Science Research Institute | Wang J.,Southwest University | Guo B.,Southwest University | And 3 more authors.
Plant and Soil | Year: 2016

Aim: The secretion of allelochemicals from plant roots plays a key role in soil sickness and soil-borne disease. The goal of this study was to investigate the role of allelopathic chemicals in Ralstonia solanacearum-infected tobacco roots. Methods: The organic acids investigated in the present study are major components of tobacco root exudates. Through a swarming assay, we assessed the chemotaxis and colonization of R. solanacearum in response to organic acids. Results: Fumaric acid was detected, and the results showed that this acid could serve as a semiochemical for attracting R. solanacearum and inducing the formation of biofilms of this species. The results also revealed that cinnamic and myristic acids play significant roles on swarming motility and chemotaxis. In addition, cinnamic, myristic and fumaric acids could enhance the expression of chemotaxis- and motility-related genes in R. solanacearum cultured in minimal medium. Furthermore, these three acids promote R. solanacearum colonization and accelerate disease progression in tobacco. Conclusion: Cinnamic, myristic and fumaric acids could serve as semiochemical attractants to induce the colonization and infection of R. solanacearum. The results of the present study enhance our understanding of the ecological effects of plant root exudates in plant-microbe interactions and help to reveal the relationship between tobacco bacterial wilt and the autotoxins and allelochemicals that accumulate from root exudates. © 2016 Springer International Publishing Switzerland

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