Chongqing Tobacco Research Institute

Chongqing, China

Chongqing Tobacco Research Institute

Chongqing, China
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Jia J.,Northwest University, China | Lu W.,Northwest University, China | Zhong C.,Northwest University, China | Zhou R.,Northwest University, China | And 7 more authors.
Frontiers in Microbiology | Year: 2017

Small RNAs (sRNAs) are important non-coding RNA regulators, playing key roles in developmental regulation, transposon suppression, environmental response, host-pathogen interaction and other diverse biological processes. However, their roles in oomycetes are poorly understood. Here, we performed sRNA sequencing and RNA sequencing of Phytophthora parasitica at stages of vegetative growth and infection of Arabidopsis roots to examine diversity and function of sRNAs in P. parasitica, a model hemibiotrophic oomycete plant pathogen. Our results indicate that there are two distinct types of sRNA-generating loci in P. parasitica genome, giving rise to clusters of 25-26 nt and 21 nt sRNAs, respectively, with no significant strand-biases. The 25-26 nt sRNA loci lie predominantly in gene-sparse and repeat-rich regions, and overlap with over 7000 endogenous gene loci. These overlapped genes are typically P. parasitica species-specific, with no homologies to the sister species P. infestans. They include approximately 40% RXLR effector genes, 50% CRN effector genes and some elicitor genes. The transcripts of most of these genes could not be detected at both the vegetative mycelium and infection stages as revealed by RNA sequencing, indicating that the 25-26 nt sRNAs are associated with efficient silencing of these genes. The 21 nt sRNA loci typically overlap with the exon regions of highly expressed genes, suggesting that the biogenesis of the 21 nt sRNAs may be dependent on the level of gene transcription and that these sRNAs do not mediate efficient silencing of homologous genes. Analyses of the published P. infestans sRNA and mRNA sequencing data consistently show that the 25-26 nt sRNAs, but not the 21 nt sRNAs, may mediate efficient gene silencing in Phytophthora. © 2017 Jia, Lu, Zhong, Zhou, Xu, Liu, Gou, Wang, Yin, Xu and Shan.

Liu Y.,Southwest University | Tang Y.,Southwest University | Zhang S.,Southwest University | Guo B.,Southwest University | And 3 more authors.
Acta Tabacaria Sinica | Year: 2016

46 isolates were assessed to explore the diversity of tobacco Ralstonia solanacearum in Chongqing by using biovar, phylotype specific multiplex PCR, and phylogenetic relationships (egl and mutS). Results showed that all tested isolates belonged to biovar 3 and phylotype I. The phylogenetic neighbor-joining tree based on partial sequences of egl and mutS genes indicated that the tested isolates were clustered into phylotype I, and were further divided into 3 sequevars, namely sequevars 15, 17, and 44. Sequevar 17 and 44 were predominant. R. solanacearum strains on tobacco showed genetic differentiation to some extent, which provided basis for studying relationship between classification and geographic areas. © 2016, Editorial Office Chinese Tobacco Society. All right reserved.

Zhang X.,Chongqing University of Technology | Wang W.,Chongqing University of Technology | Tan L.,China Tobacco Chuanyu Industrial Co. | Lu J.,Chongqing University of Technology | And 3 more authors.
Tobacco Science and Technology | Year: 2015

In order to reduce the content of starch in flue-cured tobacco leaves, three curing means were tested and compared with current curing means(CK), including low temperature high humidity longer period flue-curing (W), cellulose-aid flue-curing (M) and high temperature-induced amylase pre-activating flue-curing (BK), separately. The starch content in tobacco leaf was determined by iodine colorimetry, and the activity of amylase by 3, 5-dinitrosalicylic acid (DNS) colorimetry. The results showed that: 1) The starch contents in tobacco leaves cured by CK, W, M, BK were 6.54%, 6.06%, 5.08%, 3.13%, respectively. 2) The amylase activity in fresh tobacco leaf was 0.25 U, increased to 0.31 U after curing for 3 hours by CK. For BK, the amylase activity in tobacco leaf pre-activated by high temperature reached 0.49 U, the amylase activity during curing process exhibited a profile with two peaks. High temperature-induced amylase pre-activating flue-curing means reduced the starch content in tobacco leaf more obviously ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

Jiang H.,Chongqing Tobacco Research Institute | Xu A.,Chongqing Tobacco Research Institute | Chen D.,Chongqing Wulong Tobacco Company | Yang C.,Chongqing Tobacco Research Institute | And 2 more authors.
Acta Tabacaria Sinica | Year: 2014

Leaves of Yunyan 97 plants were removed to investigate the effect on root vigor, leaf chlorophyll content and other physiological characteristics. The number of leaves removed were 0, 2, 3 and 4 and yield, grad, economic value were also determined. Results indicated that removing leaves on lower part of plant helped enhancing root vigor, chlorophyll content, net photosynthetic rate (Pn) and transpiration rate (Tr) while delaying photosynthetic function decline. The biggest influence was from removing lower part leaf, followed by middle leaf and upper leaf. At the early stage (10 d), with the increase of number of removed fresh leaf, water use efficiency (WUE) increased. Grad and economic value were greatly improved when removing three leaves. Removing three leaves is the best agronomy practice under the ecological conditions of Wulong county. ©, 2014, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

Hou-Long J.,Henan Agricultural University | Hou-Long J.,ChongQing Tobacco Research Institute | Guo-Shun L.,Henan Agricultural University | Rui W.,Enshi Tobacco Company of Hubei Province | And 2 more authors.
Communications in Soil Science and Plant Analysis | Year: 2012

The spatial variability of soil total nutrient levels, which may be greatly affected by parent material, plays an important role in both agriculture and environment, especially with regard to soil fertility and soil quality. Little research has been done that addresses the spatial characteristics of total nutrients. Soil samples (0-20 cm) were taken from 111 points on an approximately 20-m grid in March 2009 using a global positioning system (GPS) to define sample locations. Sixteen soil total chemical properties were analyzed by classical statistical and geostatistical methods. Soil aluminum (Al), calcium (Ca), chromium (Cr), copper (Cu), nitrogen (N), sodium (Na), silicon (Si), strontium (Sr), titanium (Ti), and vanadium (V) exhibited strong spatial dependence, with the nugget-sill ratios ranging from 3.39% (Na) to 23.53% (Si). Soil barium (Ba), iron (Fe), potassium (K), manganese (Mn), phosphorus (P), and zinc (Zn) had moderate spatial dependence, with nugget-sill ratios of 3.39% to 23.53%. The spatial correlation distances varied from 15.7 m (Zn) to 286.5 m (Cr). The clearly patchy distributions indicated that significant positive correlations among Al, Ba, Fe, K, Mn, Na, Si, Ti, and V led to strong positional similarity. The interpolated maps of the nutrients showed the spatial distributions of the soil variables, which is helpful for better understanding their spatial variability and delineating the potential agricultural management zone. The soil total nutrient distribution maps could be used as the basis for site-specific fertilizer application to improve quality and increase yield of flue-cured tobacco in the study area. © 2012 Copyright Taylor and Francis Group, LLC.

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