Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education

Chongqing, China

Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education

Chongqing, China
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Tan F.,Chongqing Medical University | Wang C.,Chongqing Medical University | Wang C.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Yang C.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | And 3 more authors.
Journal of Healthcare Engineering | Year: 2017

Introduction. Osseointegration is required for prosthetic implant, but the various bone-implant interfaces of orthodontic miniscrews would be a great interest for the orthodontist. There is no clear consensus regarding the minimum amount of bone-implant osseointegration required for a stable miniscrew. The objective of this study was to investigate the influence of different bone-implant interfaces on the miniscrew and its surrounding tissue. Methods. Using finite element analysis, an advanced approach representing the bone-implant interface is adopted herein, and different degrees of bone-implant osseointegration were implemented in the FE models. A total of 26 different FE analyses were performed. The stress/strain patterns were calculated and compared, and the displacement of miniscrews was also evaluated. Results. The stress/strain distributions are changing with the various bone-implant interfaces. In the scenario of 0% osseointegration, a rather homogeneous distribution was predicted. After 15% osseointegration, the stress/strains were gradually concentrated on the cortical bone region. The miniscrew experienced the largest displacement under the no osseointegra condition. The maximum displacement decreases sharply from 0% to 3% and tends to become stable. Conclusion. From a biomechanical perspective, it can be suggested that orthodontic loading could be applied on miniscrews after about 15% osseointegration without any loss of stability. © 2017 Chao Wang et al.


Yu Y.,Chongqing Medical University | Yu Y.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Yu Y.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | Zhao T.,Chongqing Medical University | And 5 more authors.
Experimental Animals | Year: 2017

Inflammatory bowel disease (IBD) is a severe inflammatory condition in the colon. To date, clinical solutions for this disease have been limited. Mesenchymal stem cells (MSCs), multipotential cells with immune regulation and anti-inflammatory functions, have been applied to treatment of IBD. However, the therapeutic effectiveness of MSCs still needs to be improved. Here, we were interested in whether regulatory T cells (Tregs) could enhance the immune regulation function of MSCs in treatment of mouse colitis. We generated a dextran sulfate sodium (DSS)-induced IBD mouse model. Combined cell therapy with both MSCs and Tregs was able to help increase body weight and preserve a better colon morphology compared with single cell therapy with MSCs or Tregs alone. Further studies demonstrated that combined cell therapy could reduce pro-inflammatory cytokines such as TNF-α,IL-10, IFN-γ, IL-17A, IL-1β and at the same time promote CD3+ T cells apoptosis. In conclusion, our study indicates that combined cell therapy could prevent the development of colitis in a mouse model, which may lead to a new effective therapeutic approach for treatment of human IBD. © 2017 Japanese Association for Laboratory Animal Science.


He S.,Chongqing Medical University | He S.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | He S.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | Wang J.,Chongqing Medical University | And 2 more authors.
Journal of Headache and Pain | Year: 2017

Background: The present study aimed to validate of Social support and Pain Questionnaire (SPQ) for use in Chinese patients with painful temporomandibular disorders (TMD). Methods: The Chinese version of SPQ was produced by translation and cross-culturally adaptation of the original English version according to international guidelines. The Chinese version of SPQ was then distributed to a total of 118 patients with painful TMD. Reliability of the SPQ was evaluating using internal consistency and test-retest methods and validity of the SPQ was determined by construct validity and convergent validity. The exploratory factor analysis (EFA) was used to assess the construct validity of SPQ. And convergent validity was assessed by correlating the SPQ scores with the score of a global oral health question. Results: The Chinese version of SPQ has a high internal consistency (Cronbach’s alpha value, 0.926) and good test-retest reliability ((intraclass correlation coefficient (ICC), 0.784). Construct validity was evaluated by EFA, extracting one factor, accounting for 74.8% of the variance. All factor loadings of the six items had exceeded 0.80. As regards convergent validity, the SPQ showed good correlation with the global oral health question. Conclusion: These findings support that the Chinese version of SPQ can be used as a reliable and valid tool for Chinese patients with painful TMD. © 2017, The Author(s).


Yue M.,Chongqing Medical University | Aishu R.,Chongqing Medical University | Gang F.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology | Year: 2016

OBJECTIVE: This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).METHODS: Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.RESULTS: Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.CONCLUSIONS: Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.目的 探讨中药丹参主要活性成分丹酚酸B(Sal B)对人牙周膜细胞(hPDLCs)成骨分化的影响。方法 取第3代hPDLCs进行实验,运用甲基噻唑基四唑(MTT)法检测不同浓度丹酚酸B对hPDLCs增殖活性影响,通过检测碱性磷酸酶(ALP)活性、矿化结节染色、骨钙素(OCN)mRNA表达来探讨丹酚酸B对hPDLCs成骨分化的影响。结果 丹酚酸B对hPDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5 μmol·L⁻¹时均能增加hPDLCs的ALP活性和OCN mRNA表达,与OIM组比较差异有统计学意义(P<0.05);且当丹酚酸B浓度为0.5、1、5 μmol·L⁻¹时hPDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进hPDLCs成骨分化。.


Gao X.,Southwest University | Deng X.,Chongqing Medical University | Deng X.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Deng X.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | And 4 more authors.
PLoS ONE | Year: 2016

Body image distress or body dissatisfaction is one of the most common consequences of obesity and overweight. We investigated the neural bases of body image processing in overweight and average weight young women to understand whether brain regions that were previously found to be involved in processing self-reflective, perspective and affective components of body image would show different activation between two groups. Thirteen overweight (O-W group, age = 20.31±1.70 years) and thirteen average weight (A-W group, age = 20.15±1.62 years) young women underwent functional magnetic resonance imaging while performing a body image self-reflection task. Among both groups, whole-brain analysis revealed activations of a brain network related to perceptive and affective components of body image processing. ROI analysis showed a main effect of group in ACC as well as a group by condition interaction within bilateral EBA, bilateral FBA, right IPL, bilateral DLPFC, left amygdala and left MPFC. For the A-W group, simple effect analysis revealed stronger activations in Thin-Control compared to Fat-Control condition within regions related to perceptive (including bilateral EBA, bilateral FBA, right IPL) and affective components of body image processing (including bilateral DLPFC, left amygdala), as well as self-reference (left MPFC). The O-W group only showed stronger activations in Fat-Control than in Thin-Control condition within regions related to the perceptive component of body image processing (including left EBA and left FBA). Path analysis showed that in the Fat-Thin contrast, body dissatisfaction completely mediated the group difference in brain response in left amygdala across the whole sample. Our data are the first to demonstrate differences in brain response to body pictures between average weight and overweight young females involved in a body image self-reflection task. These results provide insights for understanding the vulnerability to body image distress among overweight or obese young females. © 2016 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Xu X.,Chongqing Medical University | Xu X.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Zhou J.,Chongqing Medical University | Zhou J.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | And 4 more authors.
PLoS ONE | Year: 2016

Objective: To observe dynamic changes in root resorption repair, tooth movement relapse and alveolar bone microstructure following the application of orthodontic force. Materials and Methods: Forces of 20 g, 50 g or 100 g were delivered to the left maxillary firstmolars of fifteen 10- week-old rats for 14 days. Each rat was subjected tomicro-computed tomography scanning at 0, 3, 7, 10, 14, 28 and 42 days after force removal. The root resorption crater volume, tooth movement relapse and alveolar bone microarchitecture were measured at each time point. Results: From day 3 to day 14, the root resorption volume decreased significantly in each group. In the 20-g force group, the root resorption volume gradually stabilized after 14 days, whereas in the 50-g and 100-g force groups, it stabilized after 28 days. In all groups, toothmovement relapsed significantly from day 0 to day 14 and then remained stable. From day 3 to day 10, the 20-g group exhibited faster relapse than the 50-g and 100-g groups. In all groups, the structure model index and trabecular separation decreased slowly from day 0 to day 10 and eventually stabilized. Trabecular number increased slowly from day 0 to day 7 and then stabilized. Conclusions: The initial stage of root resorption repair did not change significantly and was followed by a dramatic repair period before stabilizing. The most serious tooth movement relapse occurred immediately after the appliance was removed, and then the tooth completely returned to the original position. © 2016 Xu et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PubMed | Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Peking University
Type: Journal Article | Journal: ACS applied materials & interfaces | Year: 2016

Nanohydroxyapatite (HA) synthesized by biomimetic strategy is a promising nanomaterial as bone substitute due to its physicochemical features similar to those of natural nanocrystal in bone tissue. Inspired by mussel adhesive chemistry, a novel nano-HA was synthesized in our work by employing polydopamine (pDA) as template under weak alkaline condition. Subsequently, the as-prepared pDA-templated HA (tHA) was introduced into polycaprolactone (PCL) matrix via coelectrospinning, and a bioactive tHA/PCL composite nanofiber scaffold was developed targeted at bone regeneration application. Our research showed that tHA reinforced PCL composite nanofibers exhibited favorable cytocompatibility at given concentration of tHA (0-10 w.t%). Compared to pure PCL and traditional nano-HA enriched PCL (HA/PCL) composite nanofibers, enhanced cell adhesion, spreading and proliferation of human mesenchymal stem cells (hMSCs) were observed on tHA/PCL composite nanofibers on account of the contribution of pDA present in tHA. More importantly, tHA nanoparticles exposed on the surface of composite nanofibers could further promote osteogenesis of hMSCs in vitro even in the absence of osteogenesis soluble inducing factors when compared to traditional HA/PCL scaffolds, which was supported by in vivo test as well according to the histological analysis. Overall, our study demonstrated that the developed tHA/PCL composite nanofibers with enhanced cytocompatibility and osteogenic capacity hold great potential as scaffolds for bone tissue engineering.


Wang Q.,Chongqing Medical University | Wang Q.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Wang Q.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | Ao Y.,Chongqing Medical University | And 3 more authors.
Oncology Reports | Year: 2016

Previous studies have shown that the aberrant expression of period circadian clock 2 (Per2) is closely related to the occurrence and development of cancers,but the specific mechanism remains unclear. In the present study,we used shRNA to downregulate Per2 in oral squamous cell carcinoma (OSCC) Tca8113 cells,and then detected the alterations in cell cycle,cell proliferation and apoptosis by flow cytometric analysis and mRNA expression alterations in all the important genes in the cyclin/cyclin-dependent protein kinase (CDK)/cyclin-dependent kinase inhibitor (CKI) cell cycle network by RT-qPCR. We found that in the Tca8113 cells,after Per2 downregulation,the mRNA expression levels of cyclin A2,B1 and D1,CDK4,CDK6 and E2F1 were significantly increased (P<0.05),the mRNA expression levels of p53,p16 and p21 were significantly decreased (P<0.05),cell proliferation was significantly higher (P<0.05),apoptosis was significantly lower (P<0.05) and the number of cells in the G1/G0 phase was significantly decreased (P<0.05). The present study proves that in OSCC,clock gene Per2 plays an important role in cell cycle progression and the balance of cell proliferation and apoptosis by regulation of the cyclin/CDK/CKI cell cycle network. Further research on Per2 may provide a new effective molecular target for cancer treatments.


Singh P.,Chongqing Medical University | Wang C.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | Ajmera D.H.,Chongqing Medical University | Xiao S.S.,Chongqing Medical University | And 2 more authors.
Journal of Oral and Maxillofacial Surgery | Year: 2016

Purpose Surgically assisted mandibular arch expansion has proved to be an effective treatment modality in alleviating constriction and crowding issues. However, only mandibular symphyseal distraction osteogenesis has been in favor for the purpose of mandibular arch expansion. In addition, no relevant study has compared the biomechanical response of different osteotomy designs on mandibular expansion. The present study evaluated the effect of different osteotomy approaches and modes of loading on the expansion of the adult mandible using biomechanics. Materials and Methods To address the research purpose, 9 finite element (FE) models, including 2 novel osteotomy designs, were simulated. Stress, strain, and displacement of crown, root, and bone were calculated and compared under different osteotomy approaches and loading conditions. Results The biomechanical response envisaged by the FE models in terms of displacement on the X axis was consistent from the anterior to posterior teeth with parasymphyseal step osteotomy and a hybrid mode of force application. In addition, the amount of displacement predicted by parasymphyseal step osteotomy with the hybrid mode was greater compared with that of the other models. Conclusions The results of our study suggest parasymphyseal step osteotomy with the hybrid mode is a viable treatment option for true bony expansion in the adult mandible. © 2016 American Association of Oral and Maxillofacial Surgeons


PubMed | Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Chongqing Medical University
Type: Journal Article | Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons | Year: 2016

Surgically assisted mandibular arch expansion has proved to be an effective treatment modality in alleviating constriction and crowding issues. However, only mandibular symphyseal distraction osteogenesis has been in favor for the purpose of mandibular arch expansion. In addition, no relevant study has compared the biomechanical response of different osteotomy designs on mandibular expansion. The present study evaluated the effect of different osteotomy approaches and modes of loading on the expansion of the adult mandible using biomechanics.To address the research purpose, 9 finite element (FE) models, including 2 novel osteotomy designs, were simulated. Stress, strain, and displacement of crown, root, and bone were calculated and compared under different osteotomy approaches and loading conditions.The biomechanical response envisaged by the FE models in terms of displacement on the X axis was consistent from the anterior to posterior teeth with parasymphyseal step osteotomy and a hybrid mode of force application. In addition, the amount of displacement predicted by parasymphyseal step osteotomy with the hybrid mode was greater compared with that of the other models.The results of our study suggest parasymphyseal step osteotomy with the hybrid mode is a viable treatment option for true bony expansion in the adult mandible.

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