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Angeloni N.L.,Northwestern University | Bond C.W.,Northwestern University | Tang Y.,Chongqing Medical and Pharmaceutical College | Harrington D.A.,Rice University | And 5 more authors.
Biomaterials | Year: 2011

SHH plays a significant role in peripheral nerve regeneration and has clinical potential to be used as a regenerative therapy for the CN in prostatectomy patients and in other patients with neuropathy of peripheral nerves. Efforts to regenerate the cavernous nerve (CN), which provides innervation to the penis, have been minimally successful, with little translation into improved clinical outcomes. We propose that, Sonic hedgehog (SHH), is critical to maintain CN integrity, and that SHH delivered to the CN by novel peptide amphiphile (PA) nanofibers, will promote CN regeneration, restore physiological function, and prevent penile morphology changes that result in erectile dysfunction (ED). We performed localization studies, inhibition of SHH signaling in the CN, and treatment of crushed CNs with SHH protein via linear PA gels, which are an innovative extended release method of delivery. Morphological, functional and molecular analysis revealed that SHH protein is essential to maintain CN architecture, and that SHH treatment promoted CN regeneration, suppressed penile apoptosis and caused a 58% improvement in erectile function in less than half the time reported in the literature. These studies show that SHH has substantial clinical application to regenerate the CN in prostatectomy and diabetic patients, that this methodology has broad application to regenerate any peripheral nerve that SHH is necessary for maintenance of its structure, and that this nanotechnology method of protein delivery may have wide spread application as an in vivo delivery tool in many organs. © 2010 Elsevier Ltd.


PubMed | Chongqing Medical and Pharmaceutical College, Lanzhou University and Chongqing University of Technology
Type: Journal Article | Journal: Iranian journal of public health | Year: 2017

The publics cognition of stroke and responses to stroke symptoms are important to prevent complications and decrease the mortality when stroke occurs. The aim of study was to develop and validate the Chinese version of the Stroke Action Test (C-STAT) in a Chinese population.This study was rigorously implemented with the published guideline for the translation, adaptation and validation of instruments for the cross-cultural use in healthcare care research. A cross-sectional study was performed among 328 stroke patients and family members in the Department of Neurology in the Second Hospital of Lanzhou University, Gansu province, China in 2014.The Chinese version of the instrument showed favorable content equivalence with the source version. Values of Cronbachs alpha and test-retest reliability of the C-STAT were 0.88 and 0.86, respectively. Principal component analysis supported four-factor solutions of the C-STAT. Criterion-related validity showed that the C-STAT was a significant predictor of the 7-item stroke symptom scores (R = 0.77; The C-STAT is an intelligible and brief psychometrical tool to assess individuals knowledge of the appropriate responses to stroke symptoms in Chinese populations. It could also be used by health care providers to assess educational programs on stroke prevention.


Wang Y.,Chongqing University | Ruan C.,Chongqing University | Sun J.,Chongqing University | Zhang M.,Chongqing University | And 3 more authors.
Polymer Degradation and Stability | Year: 2011

In order to investigate the effect of different chain extenders on degradation properties of segmented polyurethanes (SPUs), three types of segmented polyurethanes (SPU-P, SPU-O and SPU-A) based on poly (d, l-lactic acid) diol, hexamethylene diisocyanate (HDI), were synthesized with three chain extenders: peperazine (PP), 1, 4-butanediol (BDO) and 1, 4-butanediamine (BDA), respectively. Thermogravimetric analysis, activation energy and in vitro degradation were used to characterize the obtained polymers, quantitatively. The results revealed that chain extender played an important role in thermal degradation and biodegradation of polyurethanes. Thermogravimetric analysis and activation energy demonstrated that SPU-O, SPU-P and SPU-A presented best, second and weakest thermostability, respectively, and the thermal degradation mechanism of three SPUs was the same and regarded as a two-stage degradation. Data of hydrolytic degradation of the polymers during 12 weeks indicated that the in vitro degradation stability of SPU-A and SPU-P was similar, but both were better than that of SPU-O. The reason for the differences among three types of SPUs was discussed in this paper. © 2011 Elsevier Ltd. All rights reserved.


Chen J.Y.,Chongqing Medical and Pharmaceutical College
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

Study on the correlation and path analysis of artemisininum comtent and related factor on Artemisiae annie. In order to obtain high artemisininum content Artemisiae annie. On the natural condition, choose 36 area where Artemisiae annie growth in three gorges reservoir area. The related factor on artemisininum content are studies through correlation and path analysis. The artemisininum content had significant correlations with biomass, over cover degree, K, P, and N in soil. P in soil had the nost positive influence on the artemisininum content with the direct path coefficinent 0.3439, over cover degree had the nost negative in fluence on the artemisininum content with the direct path coefficient -0.1421. The influence order of other factor was N in soil (0.3180), K in soil (0.2352), biomass ( -0.0084), and plant height (-0.0347). Artemisininum content in Artemisiae annie leaves are correlated with biomass, over cover degree, K in soil, P in soil, N in soil.


Zhu H.-F.,Southwest University | Wan D.,Chongqing Medical University | Luo Y.,Chongqing Medical University | Zhou J.-L.,Chongqing Medical and Pharmaceutical College | And 2 more authors.
International Journal of Biological Sciences | Year: 2010

To investigate the role and mechanism of catalpol in brain angiogenesis in a rat model of stroke, the effect of catalpol (5 mg/kg; i.p) or vehicle administered 24 hours after permanent middle cerebral artery occlusion (pMCAO) on behavior, angiogenesis, ultra-structural integrity of brain capillary endothelial cells, and expression of EPO and VEGF were assessed. Repeated treatments with Catalpol reduced neurological deficits and significantly improved angiogenesis, while significantly increasing brain levels of EPO and VEGF without worsening BBB edema. These results suggested that catalpol might contribute to infarcted-brain angiogenesis and ameliorate the edema of brain capillary endothelial cells (BCECs) by upregulating VEGF and EPO coordinately.


Zhou J.,Chongqing Medical and Pharmaceutical College
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2010

OBJECTIVE: To investigate the effect of adiponectin on hepatocyte steatosis. METHODS: L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured. RESULTS: Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells. CONCLUSIONS: Overexpression of adiponectin prevent hepatocyte steatosis.


Wang L.-J.,Chongqing Medical and Pharmaceutical College | Che K.-K.,Third People Hospital of Chongqing
Chinese Journal of New Drugs | Year: 2015

Intra-articular injection, one of the drug delivery systems, gives the drug directly into the affected joint. However, the injection solution always leaks into systemic circulation after intra-articular injection and leads to frequent administration frequency. Thus researches have been focused on sustained release drug delivery system for intra-articular injection. As one of the widely accepted and ideal sustained release drug delivery systems, microspheres are prepared by natural or synthetic polymers and have several advantages such as mature preparation technology and longer release time. This review gives an overview of microspheres as intra-articular injection drug delivery system from the type of polymers, quality evaluation, pharmacodynamics study, and safety evaluation. ©, 2015, Chinese Journal of New Drugs Co. Ltd. All right reserved.


Zhang B.,Southwest University | Chen T.,Southwest University | Chen Z.,Chongqing Medical and Pharmaceutical College | Wang M.,Southwest University | And 4 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012

A series of hesperidin derivatives were prepared and identified by IR, 1H NMR, and MS spectra. These compounds were evaluated in vitro and in vivo based on α-glucosidase inhibition, glucose consumption of HepG2 cells, and blood glucose level in streptozotocin-induced diabetic mice. The results revealed that all the compounds exhibited anti-hyperglycemic activities. The inhibition at 10-3 M of compounds 3 and 7a on α-glucosidase were 55.02% and 53.34%, respectively, as compared to 54.80% by acarbose. Treated by compound 3 and the reference drug metformin, glucose consumption of HepG2 cell were 1.78 and 2.11 mM, respectively. After the streptozotocin-induced diabetic mice were oral administrated with compound 3 at 100 mg kg-1 d-1 for 10 days, the blood glucose level of 3 treated mice (13.23 mM, P <0.05) showed significant difference when compared to model control (23.03 mM). Thus, compound 3 exhibited promising anti-hyperglycemic activity. © 2012 Elsevier Ltd. All rights reserved.


Luo P.,Chongqing Medical University | Liu Y.,Chongqing Medical and Pharmaceutical College | Xia Y.,Chongqing Medical University | Xu H.,Chongqing Medical University | Xie G.,Chongqing Medical University
Biosensors and Bioelectronics | Year: 2014

A sensitive electrochemical biosensor was developed to detect toxin A (TOA) of Clostridium difficile based on an aptamer selected by the systematic evolution of ligands using exponential enrichment and gold nanoparticles (GNPS) synthesized by Bacillus stearothermophilus. The thiolated single-stranded DNA used as the capture probe (CP) was first self-assembled on a Nafion-thionine-GNPS-modified screen-printed electrode (SPE) through an Au-thiol interaction. The horseradish peroxidase (HRP)-labeled aptamer probe (AP) was then hybridized to the complementary oligonucleotide of CP to form an aptamer-DNA duplex. In the absence of TOA, the aptamer-DNA duplex modified the electrode surface with HRP, so that an amperometric response was induced based on the electrocatalytic properties of thionine. This was mediated by the electrons that were generated in the enzymatic reaction of hydrogen peroxide under HRP catalysis. After the specific recognition of TOA, an aptamer-TOA complex was produced rather than the aptamer-DNA duplex, forcing the HRP-labeled AP to dissociate from the electrode surface, which reduced the catalytic capacity of HRP and reduced the response current. The reduction in the response current correlated linearly with the concentration of TOA in the range of 0-200. ng/mL. The detection limit was shown to be 1. nM for TOA. This biosensor was applied to the analysis of TOA and showed good selectivity, reproducibility, stability, and accuracy. © 2013 Elsevier B.V.


Li Q.,Chongqing Medical University | Li J.,Chongqing Medical and Pharmaceutical College | Lv F.,Chongqing Medical University | Li K.,Chongqing Medical University | And 2 more authors.
Journal of Clinical Neuroscience | Year: 2011

The circle of Willis is a major collateral circulation that has an important role in ischemic events. The purpose of our study was to investigate the collateral circulation in a Chinese population with 64-section multidetector CT angiography (CTA). A total of 170 patients who underwent 64-section CT angiography at The First Affiliated Hospital of Chongqing Medical University were included in our study. The morphological variations in the anterior and posterior circle of Willis were assessed in each patient. A total of 160 patients were included in the final analysis, of whom 126 (79%) demonstrated a complete anterior circle of Willis, and 50 (31%) had a complete posterior circle of Willis. A complete circle of Willis was seen in 43 of 160 participants (27%). A fetal-type posterior circle of Willis was seen in 15 (9.4%) patients. This is the first report of a CTA study of collateral circulation in a Chinese population. A higher prevalence of compromised posterior collaterals was observed in this Chinese population compared to Western and Japanese populations. © 2010 Elsevier Ltd. All rights reserved.

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