Preparation and in vitro characterization of dexamethasone-loaded poly(d,l-lactic acid) microspheres embedded in poly(ethylene glycol)-poly(Éε-caprolactone)-poly(ethylene glycol) hydrogel for orthopedic tissue engineering
Fan M.,University of Sichuan |
Guo Q.F.,University of Sichuan |
Luo J.C.,University of Sichuan |
Luo F.,University of Sichuan |
And 3 more authors.
Journal of Biomaterials Applications | Year: 2013
The corium is decreased to about half of its thickness in skin defects and wrinkles due to gravity and environment. In this study, dexamethasone/poly(d,l- lactic acid) (Mn = 160,000) microspheres were incorporated into poly(ethylene glycol)-poly(Éε-caprolactone)-poly(ethylene glycol) (Mn = 3300) hydrogel to prepare an injectable hydrogel composite. The composite was designed to increase the thickness of the corium. Dexamethasone/poly(d,l-lactic acid) microspheres were prepared by oil-in-water emulsion/solvent evaporation technique. The properties of microspheres were investigated by size distribution measurement, scanning electron microscope and x-ray diffraction. Drug loading, encapsulation efficiency, and drug delivery behavior of microspheres were also studied in detail. Cell adhesion of microspheres was investigated by NIH3T3 cell in vitro. The properties of hydrogel composite were investigated by scanning electron microscope, rheological measurements and methyl thiazolyl tetrazolium assay. Drug release from composite was determined by HPLC-UV analysis. These results suggested that poly(d,l-lactic acid) microspheres encapsulating dexamethasone embedded in poly(ethylene glycol)-poly(Éε- caprolactone)-poly(ethylene glycol) hydrogel might have prospective application in orthopedic tissue engineering field. © 2012 The Author(s).
Xue B.,University of Sichuan |
Xue B.,Chengdu University of Traditional Chinese Medicine |
Wang Y.,University of Sichuan |
Tang X.,University of Sichuan |
And 4 more authors.
Journal of Biomedical Nanotechnology | Year: 2012
The great potential of oridonin (ORI) for clinical application in cancer therapy is greatly limited due to its poor water-solubility. The purpose of this study was to increase the water solubility of oridonin using monomethoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) as drug carrier. The ORI-loaded MPEG-PCL micelles were prepared by thin film hydration method. The obtained ORI-micelles could be lyophilized into powder form, which could be re-dissolved in water to form homogeneous solution. This study showed that ORI was successfully incorporated in the core-shell structure of MPEG-PCL micelles and maintained its anticancer activity. The average particle size was 25.55±0.10 nm and the mean zeta potential was -4.71±0.05 mV. The actual drug loading and encapsulation efficiency were 7.99±0.03% and 99.51±0.34%, respectively. ORI could be released from MPEG-PCL micelles in a sustained manner in vitro. The permeation profiles of ORI from ORI-micelles and ORI water saturated solution through excised mouse skin demonstrated that ORI-micelles showed much better transdermal penetration performance than ORI water saturated solution. The prepared ORI-micelles have great potential for both direct intravascular administration and being further developed as a transdermal drug delivery system in cancer chemotherapy. Copyright © 2012 American Scientific Publishers All rights reserved.
Zeng S.,University of Sichuan |
Fu S.,University of Sichuan |
Guo G.,University of Sichuan |
Liang H.,University of Sichuan |
And 4 more authors.
Journal of Biomedical Nanotechnology | Year: 2011
In this study, nano-hydroxyapatite/poly(vinyl alcohol) (n-HA/PVA) composite membranes were prepared by solvent casting and evaporation method. The morphology, mechanical properties, water absorption behavior, contact angle measurement and biocompatibility of these membranes were examined by SEM, FTIR, XRD, MTT assay and etc. The results demonstrate that the surface of composite membranes is suitable for the adhesion and proliferation of osteogenic cells, and that n-HA and PVA are in uniform distribution when the content of n-HA is less than 20 wt%. Tests of swelling behavior and water contact angle show that the incorporation of n-HA into PVA matrix significantly reduces its hydrophilicity. Mechanical tests reveal that the addition of n-HA nanoparticles reduces tensile strength and elongation rate but increases Young's modulus of composite membranes. Cell attachment test and MTT assay prove that n-HA/PVA composite membranes have good biocompatibility. Therefore, the n-HA/PVA composite membranes possess potential application for guided bone regeneration (GBR). Copyright © 2011 American Scientific Publishers All rights reserved.
Chongqing Lummy Pharmaceutical Co. | Date: 2012-08-02
Chongqing Lummy Pharmaceutical Co. | Date: 2012-08-01
A double-headed transfusion container comprises a body portion (