Chongqing Key Laboratory of Pig Industry science

Chongqing, China

Chongqing Key Laboratory of Pig Industry science

Chongqing, China
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Huang J.X.,Chongqing Academy of Animal Science | Huang J.X.,Key Laboratory of Pig Industry science | Huang J.X.,Chongqing Key Laboratory of Pig Industry science | Qi R.L.,Chongqing Academy of Animal Science | And 15 more authors.
Genetics and Molecular Research | Year: 2014

A total of 160 Rongchang pigs (26.76 ± 1.78 kg) were randomly assigned to 5 dietary treatment groups until their body weight (BW) reached 90 kg. The diets were supplemented with 0, 0.5, 1.0, 1.5, and 2.0% conjugated linoleic acid (CLA). Our results showed that the 1.0 to 2.0% CLA-fed pigs had less back fat deposition when their BW reached 90 kg than the pigs that received less than 1% CLA. During the 30 to 60 kg growing period, 1.0, 1.5, and 2.0% CLA treatments improved pork quality by significantly reducing the pork pH (P < 0.01) and color value (P < 0.05), but they increased marble scaling (P < 0.01). Similarly, the 1.5 and 2.0% CLA-fed pigs had more marble than other pigs when their BW reached 90 kg. Furthermore, CLA significantly affected the expression of muscle fiber-type genes. The 1.5% CLA-fed pigs exhibited the highest mRNA expression of MyHC1 and MyHC2a (P < 0.05) at 60 kg BW. At 90 kg BW, the highest expression of MyHC1 and MyHC2a (P < 0.05) was found in the 2.0% CLA group. However, MyHC2x was downregulated in the CLA-fed pigs at this time. In addition, CLA supplements did not evidently alter mRNA expression of MyHC2b at all times. These results demonstrate that CLA could affect carcass traits and improve the meat quality of growing-finishing pigs by altering the expression of genes related to muscle growth and development; 1-1.5% CLA was the most appropriate CLA dose. © FUNPEC-RP.


Qi R.,Chongqing Academy of Animal Science | Qi R.,Key Laboratory of Pig Industry science | Chen Y.,Chongqing Academy of Animal Science | Peng H.,Southwest University | And 5 more authors.
Livestock Science | Year: 2015

The purpose of this study is to determine the effect of dietary conjugated linoleic acid (CLA) supplementation of pregnant and lactating sows on muscle growth and myofiber type of their piglets. There are thirty healthy, pregnant Rongchang sows with similar body condition and closed expected day of parturition for the experiment in vivo. Three experiment groups carry out with/without CLA (1%, 2% and 0%). The experiment began on 85th day of gestation and ended at 28th day after piglet birth. The results show that (1) piglets from CLA-fed sows had heavier longissimus dorsi and higher percentages of slow myofibers than control piglets (P<0.05); (2) moreover, real-time PCR showed that CLA supplementation induce increasing expression of MyHC 1, MyOG, and MyOD genes while decreasing expression of MyHC 2a, MyHC 2x, and MSTN genes in piglets. We also check the expression of genes which appear in 2 in vitro experiment. c9, t11-CLA acted to induce the differentiation of cultured pig skeletal muscle cells and up-regulates the expression levels of MyHC 1 and MyOG, but t10, c12-CLA strongly inhibited cellular differentiation and down-regulates expression of MyOG. These findings suggest that dietary CLA during early life may affect muscle development and myofiber type in pigs. © 2015 Elsevier B.V.


Qi R.,Chongqing Academy of Animal Science | Qi R.,Key Laboratory of Pig Industry science | Qi R.,Chongqing Key Laboratory of Pig Industry science | Yang F.,Chongqing Academy of Animal Science | And 10 more authors.
BMC Veterinary Research | Year: 2014

Background: Conjugated linoleic acid (CLA), a C18 fatty acid with conjugated double bonds, has been shown to serve as a powerful anti-obesity agent by several research groups, although the precise mechanism remains elusive. Previous studies showed that CLA induced apoptosis in 3T3-L1 cells and in mice. The aim of this research was to clarify the role of CLA in adipocyte apoptosis in pigs, a relevant model for obesity research.Results: Our results clearly show that back fat deposition of CLA-fed pigs was significantly lower than that of pigs in the control group. Moreover, some typical apoptotic cells were observed among the adipocytes of CLA-fed pigs. Furthermore, the CLA-fed pigs had reduced expression of the anti-apoptosis factor Bcl-2 and increased expression of the pro-apoptosis factors Bax and P53. Subsequently, increased cytochrome C was released from the mitochondria to the endochylema, and the caspase cascade was activated, resulting in cellular apoptosis. These results are consistent with the effects of Bcl-2 and Bax in regulating CLA-induced adipocyte apoptosis via the mitochondrial signaling pathway. However, the increased expression of tumor necrosis factor (TNF)-α and its receptor TNFR indicate that the effect of CLA might partly be through the death receptor signaling pathway in adipose cells.Conclusions: Our study has demonstrated that CLA reduces pig body fat deposition, an outcome that is partly meditated by apoptosis of adipose cells, and that both the mitochondrial pathway and the death receptor pathway are involved in this effect. © 2014 Qi et al.; licensee BioMed Central Ltd.


Qi R.L.,Chongqing Academy of Animal Science | Qi R.L.,Key Laboratory of Pig Industry science | Qi R.L.,Chongqing Key Laboratory of Pig Industry science | Chen Y.,Chongqing Academy of Animal Science | And 6 more authors.
Genetics and Molecular Research | Year: 2015

In this study, we evaluated the effect and possible mechanism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were collected, and real-time polymerase chain reaction (PCR) was used to determine the expression of adipocyte differentiation marker genes and associated microRNAs (miRNAs). Our results indicated that dietary CLA significantly decreased body fat deposition in the pig dorsum. The expression of adipocyte differentiation marker genes, including peroxisome proliferator-activated receptor (PPAR)-γ and CCAAT/enhancer-binding protein α (C/EBPα) were not affected, whereas the expression of fatty acid binding protein 4 (FABP4) was significantly enhanced (P < 0.05). The expression of miR-27 and miR-143 in adipose tissue was significantly decreased. Data analysis indicated a significant negative correlation between miR-27 and FABP4 expression in the dorsal subcutaneous adipose tissue. In addition, the expression of miR-143 and miR-27 exhibited a significant negative relationship with FABP4 and PPARγ in the abdominal subcutaneous adipose tissue. Thus, miRNA levels in adipose tissues could be modulated by CLA, thereby affecting adipose metabolism. © FUNPEC-RP.


Ge L.,Chongqing Academy of Animal science | Ge L.,Chongqing Key Laboratory of Pig Industry science | Li Q.,University of Manitoba | Li Q.,Manitoba Institute of Child Health | And 8 more authors.
International Journal of Nanomedicine | Year: 2015

Pelvic organ prolapse (POP) is a serious health issue affecting many adult women. Complications of POP include pelvic pressure, pelvic pain, and problems in emptying their bowels or bladder. Sometimes, POP may even cause urinary outfow obstruction and lead to bladder or kidney infections. Currently, synthetic and naturally derived materials have been chosen for treatment of POP to reduce the high recurrence rates after surgical interventions. However, existing materials for POP treatment cannot meet the clinical requirements in terms of biocompatibility, mechanics, and minimal risk of rejection. Especially, erosion in synthetic polymers and rapid degradation in natural polymers limit their further applications in clinics. To address these concerns, we report a novel POP replacement using core–sheath polystyrene/ gelatin electrospun nanofiber mesh. The outside gelatin sheath provides a hydrophilic surface and implantable integrity between host and guest, while the inner PS core offers the necessary mechanical support. The composite mesh shows graft accommodation in pelvic submucosa after implantation in vivo, as shown in hematoxylin–eosin staining and T helper cell phenotype and macrophage phenotype stainings. Qualitative analysis of inducible nitric oxide synthase, arginase, interferon-γ, and interleukin-10 gene expressions also indicates that the implanted composite mesh switches to accommodation mode 2 weeks postimplantation. Thus, these novel core–sheath polystyrene/gelatin nanofibrous membranes are promising in pelvic reconstruction. © 2015 Ge et al.


Yang F.-Y.,Chongqing Academy of Animal Science | Yang F.-Y.,Key Laboratory of Pig Industry science | Huang J.-X.,Chongqing Academy of Animal Science | Huang J.-X.,Key Laboratory of Pig Industry science | And 15 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

Western and indigenous Chinese pig breeds show obvious differences in muscle growth and meat quality, however, the underlying molecular mechanism remains unclear. The main objective of this study was to evaluate the gene expression of muscle fiber type in local Rongchang and exotic Landrace pigs at the same body weight (10~110 kg). We compared differentially expressed muscle fiber types such as types I and 2a, type 2b, as well as type 2x fibers in longissimus dorsi of Rongchang and Landrace pigs using real-time reverse-transcription PCR. Results suggested that there are varieties and existence difference in developmental regularity of fiber types of longissimus muscle between Rongchang and Landrace pigs, and the fiber composition differences mainly manifest in 80 kg. Ratio of MyHC2b type fiber in Rong Chang was significantly lower than that of Landrace pig. These results may provide valuable information for understanding the molecular mechanism responsible for breed specific differences in growth performance and meat quality.


Dai R.-G.,Chongqing Academy of Animal Science | Dai R.-G.,Key Laboratory of Pig Industry science | Zhou X.-R.,Key Laboratory of Pig Industry science | Zhou X.-R.,Chongqing Key Laboratory of Pig Industry science | And 7 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

Performance of chicken with different concentrations of CO2 in the henhouse was studied on three hundred and twenty 21-day-old Ai Weiyin broiler chickens, randomly divided into 5 groups with different CO2 concentration (3,000 ppm, 5,000 ppm, 8,000 ppm, 12,000 ppm and 15,000 ppm). Production performance was detected. There was no significant difference on performance below 12,000 ppm groups of CO2 concentrations, but the chicken daily weight gain was significantly lower and the feed gain ratio significantly higher in more than 12,000 ppm of CO2 group. It significantly affected the performance of chicken when the concentrations of CO2 were above 12,000 ppm. The concentration of CO2 in the henhouse should be less than 12,000 ppm for good performance.


Zhai S.-Q.,Chongqing Academy of Animal Science | Zheng H.,Chongqing Academy of Animal Science | Zheng H.,Key Laboratory of Pig Industry science | Yang L.,Chongqing Academy of Animal Science | And 8 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

In recent years it has become a research hotspot to isolate and clone differentially expressed genes by the development of molecular biology and genomics project. It can give us a better understanding of the molecular mechanism of life activity based on the different activities in a variety of related genes for screening and research. Differential gene expression separation technologies which commonly used in recent years were compared in this paper.

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