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Yu Y.,PLA Fourth Military Medical University | Yu Y.,Chongqing Medical University | Yu Y.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Yu Y.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | And 16 more authors.
Molecular Therapy | Year: 2017

Bone marrow-derived mesenchymal stem cells (MSCs) have been recently used in clinical treatment of inflammatory diseases. Practical strategies improving the immunosuppressive property of MSCs are urgently needed for MSC immunotherapy. In this study, we aimed to develop a microRNA-based strategy to improve MSC immunotherapy. Bioinformatic analysis revealed that let-7a targeted the 3′ UTR of mRNA of Fas and FasL, both of which are essential for MSCs to induce T cell apoptosis. Knockdown of let-7a by specific inhibitor doubled Fas and Fas ligand (FasL) protein levels in MSCs. Because Fas attracts T cell migration and FasL induces T cell apoptosis, knockdown of let-7a significantly promoted MSC-induced T cell migration and apoptosis in vitro and in vivo. Importantly, MSCs knocked down of let-7a were more efficient to reduce the mortality, prevent the weight loss, suppress the inflammation reaction, and alleviate the tissue lesion of experimental colitis and graft-versus-host disease (GVHD) mouse models. In conclusion, knockdown of let-7a significantly improved the therapeutic effect of MSC cytotherapy on inflammatory bowel diseases and GVHD. With high safety and convenience, knockdown of let-7a is a potential strategy to improve MSC therapy for inflammatory diseases in clinic. © 2017 The American Society of Gene and Cell Therapy


Gao X.,Southwest University | Deng X.,Chongqing Medical University | Deng X.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Deng X.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | And 4 more authors.
PLoS ONE | Year: 2016

Body image distress or body dissatisfaction is one of the most common consequences of obesity and overweight. We investigated the neural bases of body image processing in overweight and average weight young women to understand whether brain regions that were previously found to be involved in processing self-reflective, perspective and affective components of body image would show different activation between two groups. Thirteen overweight (O-W group, age = 20.31±1.70 years) and thirteen average weight (A-W group, age = 20.15±1.62 years) young women underwent functional magnetic resonance imaging while performing a body image self-reflection task. Among both groups, whole-brain analysis revealed activations of a brain network related to perceptive and affective components of body image processing. ROI analysis showed a main effect of group in ACC as well as a group by condition interaction within bilateral EBA, bilateral FBA, right IPL, bilateral DLPFC, left amygdala and left MPFC. For the A-W group, simple effect analysis revealed stronger activations in Thin-Control compared to Fat-Control condition within regions related to perceptive (including bilateral EBA, bilateral FBA, right IPL) and affective components of body image processing (including bilateral DLPFC, left amygdala), as well as self-reference (left MPFC). The O-W group only showed stronger activations in Fat-Control than in Thin-Control condition within regions related to the perceptive component of body image processing (including left EBA and left FBA). Path analysis showed that in the Fat-Thin contrast, body dissatisfaction completely mediated the group difference in brain response in left amygdala across the whole sample. Our data are the first to demonstrate differences in brain response to body pictures between average weight and overweight young females involved in a body image self-reflection task. These results provide insights for understanding the vulnerability to body image distress among overweight or obese young females. © 2016 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Xu X.,Chongqing Medical University | Xu X.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Zhou J.,Chongqing Medical University | Zhou J.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | And 4 more authors.
PLoS ONE | Year: 2016

Objective: To observe dynamic changes in root resorption repair, tooth movement relapse and alveolar bone microstructure following the application of orthodontic force. Materials and Methods: Forces of 20 g, 50 g or 100 g were delivered to the left maxillary firstmolars of fifteen 10- week-old rats for 14 days. Each rat was subjected tomicro-computed tomography scanning at 0, 3, 7, 10, 14, 28 and 42 days after force removal. The root resorption crater volume, tooth movement relapse and alveolar bone microarchitecture were measured at each time point. Results: From day 3 to day 14, the root resorption volume decreased significantly in each group. In the 20-g force group, the root resorption volume gradually stabilized after 14 days, whereas in the 50-g and 100-g force groups, it stabilized after 28 days. In all groups, toothmovement relapsed significantly from day 0 to day 14 and then remained stable. From day 3 to day 10, the 20-g group exhibited faster relapse than the 50-g and 100-g groups. In all groups, the structure model index and trabecular separation decreased slowly from day 0 to day 10 and eventually stabilized. Trabecular number increased slowly from day 0 to day 7 and then stabilized. Conclusions: The initial stage of root resorption repair did not change significantly and was followed by a dramatic repair period before stabilizing. The most serious tooth movement relapse occurred immediately after the appliance was removed, and then the tooth completely returned to the original position. © 2016 Xu et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Wang C.,Chongqing Medical University | Wang C.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Zhang W.,Lanzhou Hospital of Stomatology | Ajmera D.H.,Chongqing Medical University | And 5 more authors.
Biomechanics and Modeling in Mechanobiology | Year: 2015

Dental implants have to be placed with the long axis in different angulations due to the change in bone morphology. The objective of this study was to investigate the different bone remodeling response induced by the tilted dental implants and to assess whether it could lead to bone loss and implant failure. In this study, bone remodeling due to palato-labially inclined dental implants placed in the anterior maxillary incisor region was simulated. CT-based finite element models of a maxillary bone with dental implants were created herein. Five dental implants were placed at (Formula presented.), (Formula presented.), (Formula presented.), (Formula presented.) and (Formula presented.), respectively. The remodeling progression was recorded and compared. Model (Formula presented.) (palatal side) shows the highest bone density values, but the inclined implant at (Formula presented.) (labial side) leads to significant bone loss. From a biomechanical perspective, it is speculated that a palatally inclined implant is more likely to enhance the bone density in the maxillary anterior region, but labial inclination of implant could jeopardize its stability. © 2015 Springer-Verlag Berlin Heidelberg


PubMed | Chongqing Key Laboratory of Oral Diseases and Biomedical science, Chongqing Medical University and Chong Qing Obstetrics and Gynecology Hospital
Type: Journal Article | Journal: Nutrients | Year: 2016

Amino acids have an important role in the pre and post implantation of placenta and embryo development. l-type amino-acid transporter 1 (lat1) is responsible for the transportation of large neutral amino acids and is mainly expressed in human fetal liver, placenta, brain, etc. This study is the first to investigate the expression of lat1 in the early pregnancy of mouse uteri and its role in the process of decidualization. Endometrial stromal cells of a mouse model were used to evaluate decidualization from Day 4-8 of pregnancy in vitro followed by lat1 knock down by small interfering RNA and by a competitive inhibitor of Leucine transport 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). The effects of lat1 on decidualization in vivo were assessed by injecting BCH into the uterine horns. The mRNA and protein expressions of lat1 in the implantation sites were higher than that in the inter-implantation sites and were localized in the luminal and gland epithelium, stromal and decidual cells. Its increased expression (


Zhang H.,Chongqing Medical University | Li L.,Chongqing Medical University | Dong Q.,Chongqing Medical University | Wang Y.,Chongqing Medical University | And 6 more authors.
Cellular Physiology and Biochemistry | Year: 2015

Background/Aims: BMP9 is highly capable of promoting osteogenic differentiation of mesenchymal stem cells (MSCs) although the molecular mechanism involved is largely unknown. Here, we explored the detail role of PKA/CREB signaling in BMP9-induced osteogenic differentiation. Methods: Activation status of PKA/CREB signaling is assessed by nonradioactive assay and Western blot. Using PKA inhibitors and a dominant negative protein of CREB (A-CREB), we investigated the effect of PKA/CREB signaling on BMP9-induced osteogenic differentiation. Results: We found that BMP9 promotes PKA activity and enhances CREB phosphorylation in MSCs. BMP9 is shown to down-regulate protein kinase A inhibitor γ (PKIγ) expression. We demonstrated that PKA inhibitors suppress BMP9-induced early osteogenic marker alkaline phosphatase (ALP) activity in MSCs as well as late osteogenic markers osteopontin (OPN), osteocalcin (OCN) and matrix mineralization. We found that PKA inhibitor reduces BMP9-induced Runx2 activation and p38 phosphorylation in MSCs. Lastly, interference of CREB function by A-CREB decreased BMP9-induced osteogenic differentiation as well. Conclusion: Our results revealed that BMP9 may activate PKA/CREB signaling in MSCs through suppression of PKIγ expression. It is noteworthy that inhibition of PKA/CREB signaling may impair BMP9-induced osteogenic differentiation of MSCs, implying that activation of PKA/CREB signaling is required for BMP9 osteoinductive activity. © 2015 S. Karger AG, Basel.


Ji Y.,Chongqing Medical University | Ji Y.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Wang L.,Chongqing Medical University | Wang L.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | And 9 more authors.
Dental Materials | Year: 2014

Objectives. Osteoporosis is one of the most common bone diseases in the world and resultsfrom an imbalance of bone cell functions. In the process of guided bone regeneration, osteo-porosis weakens the bonding strength between scaffold and bone. Naringin is evidenced tobe effective for the treatment of osteoporosis and bone resorption and the aim was to exploremethods and benefits of its incorporation.Methods. In this study, naringin was incorporated in the electrospun nanoscaffold containingpoly(ε-caprolactone) (PCL) and poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-b-PCL).Results. The nanoscaffold demonstrated unchanged chemical structure, improvedhydrophilicity, thinner and more uniform nanofibers by Fourier-transform infrared spec-troscopy, contact angle measurement and scanning electron microscopy. The nanoscaffoldalso showed faster degradation rate and controlled-release of naringin. Osteoblast-nanoscaffold interactions were studied by the evaluation of adhesion, proliferation,differentiation of MC3T3-E1 osteoblasts and mineralization of ECM on the nanoscaffolds.Meanwhile, the response of osteoclasts to nanoscaffolds was evaluated in a mouse calvarialcritical size defect organ culture model. The osteoclasts around the bone defect were shownby tartrate resistant acid phosphatase staining.Significance. The results demonstrated that controlled-release naringin nanoscaffolds sup-ported greater osteoblast adhesion, proliferation, differentiation, and mineralization and suppressed osteoclast formation. © 2014 Academy of Dental Materials.


He S.-L.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Wang J.-H.,Chongqing Key Laboratory of Oral Diseases and Biomedical science
Quality of Life Research | Year: 2015

Objectives: The aim of this study was to validate the short form of the Dentine Hypersensitivity Experience Questionnaire (DHEQ-15) in Chinese patients with dentine hypersensitivity (DH). Methods: In total, 200 patients with DH were recruited to complete the questionnaire. The reliability of the DHEQ-15 was evaluated using internal consistency and test–retest methods. The exploratory factor analysis (EFA) was conducted to identify domains of the DHEQ-15. Convergent validity was determined by analyzing the correlation between DHEQ-15 subscale scores and the global rating of oral health question. Results: Cronbach’s alpha value (internal reliability) for the total DHEQ-15 score was 0.934 (higher to the original DHEQ-15’ 0.924), and the intraclass correlation coefficient value (test–retest reliability) was 0.894 (lower to the original DHEQ-15’ 0.939). The EFA identified three components with eigenvalues >1, explaining 53.0, 17.1, and 12.3 % of the variance, respectively, accounting for a total of 82.4 % of the variance. The three components named ‘restrictions’ (three items), ‘changes in eating habits’ (six items), and ‘emotions and identity’ (six items). In terms of convergent validity, the DHEQ-15 subscale was significant highly negatively correlated to the global oral health rating. Conclusions: The results provide initial evidence that the DHEQ-15 can be properly used for assessment of patients with DH in China. © 2014, Springer International Publishing Switzerland.


He S.-L.,Chongqing key Laboratory of Oral Diseases and Biomedical science | Wang J.-H.,Chongqing key Laboratory of Oral Diseases and Biomedical science
Quality of Life Research | Year: 2015

Objectives: The aim of this study was to evaluate the reliability and validity of the Chinese version of the Oral Health Impact Profile for edentulous subjects (OHIP-EDENT-C). Methods: After translation and cross-cultural adaptation following international guidelines, 162 complete denture wearers were recruited to complete the questionnaire. The reliability of the OHIP-EDENT-C was evaluated using internal consistency and test–retest methods. The validity of the OHIP-EDENT-C was analyzed by construct validity and convergent validity. Construct validity was determined based on factor analysis and convergent validity by analyzing the correlation between OHIP-EDENT-C subscale scores and the global question. Results: Cronbach’s alpha value (internal reliability) for the total OHIP-EDENT-C score was 0.972, and the intraclass correlation coefficient value (test–retest reliability) was 0.763 (similar to the original OHIP-EDENT). Construct validity was determined by factor analysis, extracting five factors, accounting for 74.31 % of the variance. All items had factor loadings above 0.40. In terms of convergent validity, the OHIP-EDENT-C subscale was significantly highly correlated to the global question. Conclusion: The results suggest that the OHIP-EDENT-C is a reliable and valid measure to evaluate OHRQoL for edentulous subjects in China. © 2014, Springer International Publishing Switzerland.


Wang Q.,Chongqing Medical University | Wang Q.,Chongqing Key Laboratory of Oral Diseases and Biomedical science | Wang Q.,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education | Ao Y.,Chongqing Medical University | And 3 more authors.
Oncology Reports | Year: 2016

Previous studies have shown that the aberrant expression of period circadian clock 2 (Per2) is closely related to the occurrence and development of cancers,but the specific mechanism remains unclear. In the present study,we used shRNA to downregulate Per2 in oral squamous cell carcinoma (OSCC) Tca8113 cells,and then detected the alterations in cell cycle,cell proliferation and apoptosis by flow cytometric analysis and mRNA expression alterations in all the important genes in the cyclin/cyclin-dependent protein kinase (CDK)/cyclin-dependent kinase inhibitor (CKI) cell cycle network by RT-qPCR. We found that in the Tca8113 cells,after Per2 downregulation,the mRNA expression levels of cyclin A2,B1 and D1,CDK4,CDK6 and E2F1 were significantly increased (P<0.05),the mRNA expression levels of p53,p16 and p21 were significantly decreased (P<0.05),cell proliferation was significantly higher (P<0.05),apoptosis was significantly lower (P<0.05) and the number of cells in the G1/G0 phase was significantly decreased (P<0.05). The present study proves that in OSCC,clock gene Per2 plays an important role in cell cycle progression and the balance of cell proliferation and apoptosis by regulation of the cyclin/CDK/CKI cell cycle network. Further research on Per2 may provide a new effective molecular target for cancer treatments.

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