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Jia Q.,Chongqing Medical University | Jia Q.,Chongqing Key Laboratory of Cytomics | Yang Y.,Chongqing Medical University | Yang Y.,Chongqing Key Laboratory of Cytomics | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Purpose: Pathological evidence has continually supported the prognostic value of tumour-infiltrating T lymphocytes for several solid tumours across diverse patient cohorts. To investigate the clinical relevance of tumour-infiltrating memory T lymphocytes, we investigated relevant publications to identify the significance of memory tumour-infiltrating T lymphocytes (TILs) in predicting survival in cancer patients and analysed the influence of variations in the tumour stage and the infiltrated location thereon. Methods: Relevant publications that assessed the clinical relevance of memory TILs to the patient’s overall survival (OS) were investigated. Disease-free survival (DFS) was also evaluated where possible. Hazard ratios (HR) and 95% confidence intervals (CI) were assessed using a random-effects model. Heterogeneity was investigated with stratified analysis and I2 statistics. Results: In total, 16 relevant publications, including 4248 cancer patients, were analysed. In the pooled analysis, intra-tumour accumulation of memory TILs correlated positively with favourable clinical outcomes for both OS (pooled HR, 2.06; 95% CI, 1.76-2.40) and DFS (pooled HR, 2.31; 95% CI, 1.67-3.19). Various effects of the tumour stage and the anatomical region where the cells infiltrated were identified in survival prediction, using memory TILs. Conclusions: Overall, infiltration of memory TILs can serve as a biomarker for survival prediction in cancer. Additional heterogeneous effects of the associated factors should be considered when categorizing high-risk patients. © 2015, Int J Clin Exp Med. All Rights Reserved.

Li Y.,Chongqing Medical University | Li Y.,Chongqing Key Laboratory of Cytomics | Wang Y.,Chongqing Medical University | Wang Y.,Chongqing Key Laboratory of Cytomics | And 34 more authors.
Immunity | Year: 2016

Dendritic cells (DCs) orchestrate complex membrane trafficking through an interconnected transportation network linked together by Rab GTPases. Through a tandem affinity purification strategy and mass spectrometry, we depicted an interactomic landscape of major members of the mammalian Rab GTPase family. When complemented with imaging tools, this proteomic analysis provided a global view of intracellular membrane organization. Driven by this analysis, we investigated dynamic changes to the Rab32 subnetwork in DCs induced by L. monocytogenes infection and uncovered an essential role of this subnetwork in controlling the intracellular proliferation of L. monocytogenes. Mechanistically, Rab32 formed a persistent complex with two interacting proteins, PHB and PHB2, to encompass bacteria both during early phagosome formation and after L. monocytogenes escaped the original containment vacuole. Collectively, we have provided a functional compartmentalization overview and an organizational framework of intracellular Rab-mediated vesicle trafficking that can serve as a resource for future investigations. © 2016 Elsevier Inc.

Li Y.,Chongqing Medical University | Li Y.,Chongqing Key Laboratory of Cytomics | Jia Q.,Chongqing Medical University | Jia Q.,Chongqing Key Laboratory of Cytomics | And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Abstract Renal cell carcinoma (RCC) is a common urological cancer with a poor prognosis. A recent cohort study revealed that the median survival of RCC patients was only 1.5 years and that <10% of the patients in the study survived up to 5 years. In tumor development, Rab GTPase are known to play potential roles such as regulation of cell proliferation, migration, invasion, communication, and drug resistance in multiple tumors. However, the correlation between Rabs expression and the occurrence, development, and metastasis of RCC remains unclear. In this study, we analyzed the transcriptional levels of 52 Rab GTPases in RCC patients. Our results showed that high levels of Rab25 expression were significantly correlated with RCC invasion classification (P < 0.01), lymph-node metastasis (P < 0.001), and pathological stage (P < 0.01). Conversely, in 786-O and A-498 cells, knocking down Rab25 protein expression inhibited cell proliferation, migration, and invasion. Our results also demonstrated that Rab25 is a target gene of let-7d, and further suggested that Rab25 upregulation in RCC is due to diminished expression of let-7d. These findings indicate that Rab25 might be a novel candidate molecule involved in RCC development, thus identifying a potential biological therapeutic target for RCC. © 2015 Elsevier Inc. All rights reserved.

Li Y.,Chongqing Medical University | Li Y.,Chongqing Key Laboratory of Cytomics | Lian H.,Chongqing Medical University | Lian H.,ChengDu Military General Hospital | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Non-small cell lung cancer (NSCLC) is a common malignant disease, and in ∼10-20% of patients, pleural effusion is the first symptom. The pleural effusion proteome contains information on pulmonary disease that directly or indirectly reflects pathophysiological status. However, the proteome of pleural effusion in NSCLC patients is not well understood, nor is the variability in protein composition between malignant and benign pleural effusions. Here, we investigated the different proteins in pleural effusions from NSCLC and tuberculosis (TB) patients by using nano-scale liquid chromatography-tandem mass spectrometry (nLC-MS/MS) analysis. In total, 363 proteins were identified in the NSCLC pleural effusion proteome with a low false discovery rate (<1%), and 199 proteins were unique to NSCLC. The proteins in the NSCLC patients' pleural effusion were involved in cell adhesion, proteolysis, and cell migration. Furthermore, interleukin 1 alpha (IL1A), a protein that regulates tumor growth, angiogenesis, and metastasis, was significantly more abundant in the NSCLC group compared to the TB group, a finding that was validated with an ELISA assay. © 2014 Elsevier Inc. All rights reserved.

Li Y.,Chongqing Medical University | Li Y.,Chongqing Key Laboratory of Cytomics | Jia Q.,Chongqing Medical University | Jia Q.,Chongqing Key Laboratory of Cytomics | And 8 more authors.
Medical Oncology | Year: 2015

Gastric cancer (GC) is the fourth most common cancer worldwide and is associated with a low 5-year survival rate of <24 % due to the tendency of early invasion and metastasis. Rab GTPases, which are master regulators of intracellular trafficking, have been shown to a play new role in the control of multiple tumor-related processes, including cell migration, invasion, proliferation, communication, and drug resistance. Here, we analyzed the mRNA expression levels of 63 Rab GTPases in samples from GC patients. Our data demonstrated that the expression level of Rab40b was significantly correlated with GC invasion classification (P < 0.01), lymph node metastasis (P < 0.01), and pathological stage (P < 0.01). High Rab40b mRNA expression was also correlated with shorter overall survival in patients with GC (P < 0.05). Moreover, knockdown of Rab40b protein reduced the migration and invasion of GC cells, while overexpression of Rab40b significantly promoted GC cell metastasis in nude mice. Our results also showed that Rab40b is a target gene of miR-204 and further demonstrated that Rab40b is negatively correlated with miR-204 in GC tissues. These findings indicate that Rab40b might be a novel prognostic marker and a candidate biological therapeutic target for GC. © 2015, Springer Science+Business Media New York.

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