Chongqing Key Laboratory of Chinese Medicine Resources

Chongqing, China

Chongqing Key Laboratory of Chinese Medicine Resources

Chongqing, China

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Chen D.-X.,Chongqing Academy of Chinese Materia Medica | Chen D.-X.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica | Chen D.-X.,Chongqing Key Laboratory of Chinese Medicine Resources | Chen D.-X.,Chinese Academy of Sciences | And 16 more authors.
Zhongguo Zhongyao Zazhi | Year: 2017

The genetic diversity and genetic relationship among four medicinal species of Coptis were detected by the approach of sequence-related amplified polymorphism (SCoT). The associated genetic parameters were calculated by POPGENE1.31. The systematic diagram of genetic relationship were clustered by TREECONW. The results showed that a total of 434 bands were produced by using 28 primers, of which 430 were polymorphic loci. There was a high level of genetic diversity among species (PPB = 99.1%,Na = 1.990 6,Ne = 1.329 3, H = 0.212 2, I = 0.337 8). However, genetic diversity was lower within species, the average of genetic parameters wasPPB = 16.8%,NB = 1.168 2, Ne = 1.073 0,H = 0.043 7, I = 0.067 7. The Nei's genetic differentiation coefficient was 0.794 0, that indicated that most of the genetic variation existed among species. By clustering analysis, different individuals gathered in the same group. The results confirmed that SCoT marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Coptis.


Chen D.X.,Chongqing Academy of Chinese Materia Medica | Chen D.X.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica | Chen D.X.,Chongqing Key Laboratory of Chinese Medicine Resources | Chen D.X.,Chinese Academy of Sciences | And 12 more authors.
Genetics and Molecular Research | Year: 2015

Lonicera macranthoides is an important traditional Chinese herb. The lack of information regarding the genetic structure and genetic relationships among its cultivars has hindered the conservation and utilization of this resource. This study used start codon targeted markers to assess the genetic diversity and other genetic characteristics of five single-variety L. macranthoides populations in China. Using 22 primers produced a total of 266 bands, of which 227 were polymorphic, indicating a high level of polymorphism. At the species level, genetic diversity was high: percentage of polymorphic loci (PPB) = 85.34%, effective number of alleles (NE) = 1.3479, Nei’s gene diversity (H) = 0.2075, and Shannon’s information index (Hsp, species level) = 0.3198. However, at the varietal population level, genetic diversity was lower, with averages of: PPB = 19.74%, NE = 1.0946, H = 0.0561, Hpop = 0.0850 (population level). Nei’s genetic differentiation coefficient was 0.7319, which is consistent with Shannon’s population genetic differentiation coefficient (0.7324). This indicates that most of the genetic variation in this species exists among the varietal populations. The differentiation among varieties may have been caused by artificial selection, mode of reproduction, and barriers to gene flow (0.1831). The genetic similarity coefficient ranged from 0.7222 to 0.9419. Phylogenetic analysis showed the five varieties to form two major clades. Results suggest that cultivar breeders should strengthen the exchange of germplasm and increase the mutual penetration of useful genes, which would broaden the hereditary basis of L. macranthoides. © FUNPEC-RP.

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