Chongqing Key Laboratory for Proteomics of Diseases

Chongqing, China

Chongqing Key Laboratory for Proteomics of Diseases

Chongqing, China
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Zhou J.,Chongqing Medical University | Zhou J.,Chongqing Key Laboratory for Proteomics of Diseases | Wang X.,Chongqing Medical University | Wang X.,Urumqi General Hospital of PLA | And 26 more authors.
Scandinavian Journal of Immunology | Year: 2012

Thymus grafts made up of mixed syngeneic and xenogeneic thymus tissues could induce donor-specific tolerance to xenografts with no development of autoimmune syndrome (AIS). But the requirements for the simultaneous presentation of tissue antigens from both species in the process of T cell development in thymus grafts have not hitherto been defined. To do this, we setup a model in which xenothymus grafts from F344 rats were heterotopically implanted into BALB/c nude mice carrying syngeneic thymus grafts, and the grafts were either mixed together or spatially separately; next, we examined the induction of donor-specific tolerance, any pathological changes and the distribution of T lymphocytes. In contrast to the mixed thymus grafts, spatially separated thymus transplants could neither induce a long-term tolerance to skin grafts nor prevent AIS completely. 51Cr-labelled cell-tracing experiments showed that mature peripheral T cells could re-enter into both kinds of thymus grafts, while the T cells isolated from the syngeneic thymus tended to concentrate in the xenothymus grafts. Hence, our data suggest that the immune tolerance induced by mixed thymus transplants could be partially reversed if the thymus tissues from donors and recipients were segregated by spatial telorism. The uneven recirculation of mature T cells might imply that T cells get retolerized within the thymus grafts. © 2012 The Authors. Scandinavian Journal of Immunology © 2012 Blackwell Publishing Ltd.


Zhou J.,Chongqing Medical University | He W.,Chongqing Medical University | He W.,Chongqing Key Laboratory for Proteomics of Diseases | Luo G.,Chongqing Medical University | And 2 more authors.
Archivum Immunologiae et Therapiae Experimentalis | Year: 2013

Transplantation of allogeneic or xenogeneic skin grafts can evoke strong immune responses that lead to acute rejection of the graft tissues. In this process, donor-derived dendritic cells play crucial roles in the triggering of such immune responses. Both the innate and acquired host immune systems participate in graft rejection. At present, the rejection of skin grafts cannot be well-controlled by ordinary systemic immunosuppression therapy. Although several strategies for the long-term survival of allogeneic or xenogeneic skin grafts have been demonstrated in animal models, the induction of long-term tolerance to skin grafts is still a great challenge in clinical settings. In this article, we review the progress in the understanding of immune responses to skin grafts and discuss the possible methods that can decrease the immunogenicity of graft tissues and improve the survival of skin grafts, especially those included in preoperative pre-treatments. © 2013 L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.


Huang Z.,Chongqing Medical University | Huang Z.,Chongqing Key Laboratory for Proteomics of Diseases | Yang J.,Chongqing Medical University | Yang J.,Chongqing Key Laboratory for Proteomics of Diseases | And 24 more authors.
PLoS ONE | Year: 2010

How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas. © 2010 Huang et al.

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