Chongqing Key Laboratory for Oral Diseases and Biomedical science

Chongqing, China

Chongqing Key Laboratory for Oral Diseases and Biomedical science

Chongqing, China

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Wu X.,Chongqing Medical University | Wu X.,Chongqing key Laboratory for Oral Diseases and Biomedical science | Wang L.,Chongqing Medical University | Wang L.,Chongqing key Laboratory for Oral Diseases and Biomedical science | And 4 more authors.
Microscopy Research and Technique | Year: 2014

The ability of bone repair scaffolds to form bone is traditionally evaluated using cell culture and animal experiments. Mouse calvarial organ culture maintains the natural cell-to-cell and cell-to-matrix relationships as well as the anatomical order, and this model has been used to study the biological behavior of intramembranous bones. The aim of this study was to evaluate the potential of mouse calvarial organ culture to be used as an in vitro model to study the bone regenerative ability of bone repair polymer scaffolds. Critical size defects (CSD) were created in the parietal bones. Electrospun poly(ε-caprolactone) scaffolds were placed into one group of defects. The remaining defects served as a control. The bones were cultured for 38 days and analyzed with μCT, phase-contrast microscopy, dissecting microscopy, scanning electron microscopy, and energy-dispersive X-ray analyses. This organ culture technique is easily available and could permit researchers to quickly establish a valuable database of candidate bone repair scaffolds. Microsc. Res. Tech. 77:1037-1043, 2014. © 2014 Wiley Periodicals, Inc.


Pang L.,Chongqing Medical University | Pang L.,Chongqing Key Laboratory for Oral Diseases and Biomedical science | Zhao X.,Chongqing University | Liu W.,Chongqing Medical University | And 5 more authors.
Nutrients | Year: 2015

Bear bile was used as a traditional medicine or tonic in East Asia, and ursodeoxycholic acid (UDCA) is the most important compound in bear bile. Further, synthetic UDCA is also used in modern medicine and nutrition; therefore, its further functional effects warrant research, in vitro methods could be used for the fundamental research of its anticancer effects. In this study, the apoptotic effects of UDCA in human oral squamous carcinoma HSC-3 cells through the activation of caspases were observed by the experimental methods of MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, DAPI (4’,6-diamidino-2-phenylindole) staining, flow cytometry analysis, RT-PCR (reverse transcription-polymerase chain reaction) assay and Western blot assay after HSC-3 cells were treated by different concentrations of UDCA. With 0 to 400 μg/mL UDCA treatment, UDCA had strong growth inhibitory effects in HSC-3 cells, but had almost no effect in HOK normal oral cells. At concentrations of 100, 200 and 400 μg/mL, UDCA could induce apoptosis compared to untreated control HSC-3 cells. Treatment of 400 μg/mL UDCA could induce more apoptotic cancer cells than 100 and 200 μg/mL treatment; the sub-G1 DNA content of 400 μg/mL UDCA treated cancer cells was 41.3% versus 10.6% (100 μg/mL) and 22.4% (200 μg/mL). After different concentrations of UDCA treatment, the mRNA and protein expressions of caspase-3, caspase-8, caspase-9, Bax, Fas/FasL (Fas ligand), TRAIL (TNF-related apoptosis-inducing ligand), DR4 (death receptor 4) and DR5 (death receptor 5) were increased in HSC-3 cells, and mRNA and protein expressions of Bcl-2 (B-cell lymphoma 2), Bcl-xL (B-cell lymphoma-extra large), XIAP (X-linked inhibitor of apoptosis protein), cIAP-1 (cellular inhibitor of apoptosis 1), cIAP-2 (cellular inhibitor of apoptosis 2) and survival were decreased. Meanwhile, at the highest concentration of 400 μg/mL, caspase-3, caspase-8, caspase-9, Bax, Fas/FasL, TRAIL, DR4, DR5, and IκB-α expression levels were the highest, and Bcl-2, Bcl-xL, XIAP, cIAP-1, cIAP-2, survival, and NF-κB expression levels were the lowest. These results proved that UDCA could induce apoptosis of HSC-3 cancer cells through caspase activation, and the higher concentration of UDCA had stronger effects in vitro. UDCA might be a good nutrient for oral cancer prevention. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


He S.-L.,Chongqing Key Laboratory for Oral Diseases and Biomedical science | He S.-L.,Chongqing Medical University | Wang J.-H.,Chongqing Key Laboratory for Oral Diseases and Biomedical science | Wang J.-H.,Chongqing Medical University | And 2 more authors.
Quality of Life Research | Year: 2013

Objectives The aim of this study was to validate the Chinese version of the Summated Xerostomia Inventory (SXI). Methods The English SXI was translated into Chinese, cross-cultural adaptation and pilot tested. The final Chinese version of SXI was tested in a consecutive sample of 212 patients with xerostomia. The reliability of the Chinese version of SXI was determined through internal consistency and test-retest methods. The construct validity of SXI was analysed by content validity, construct validity, and convergent validity. Results Cronbach's alpha value for the SXI score was 0.798, and the test-retest intraclass correlation coefficient value for the SXI score was 0.837. Construct validity was proved by the presence of one-factor structure that accounted for 57.68 % of the variance and fitted well into the model. The correlation between the total score of the SXI and the global oral health question was 0.75, indicating very good correlation (P \ 0.01). Conclusion This study provided preliminary evidence concerning validity and reliability of the Chinese version of the SXI. The results provide initial evidence that the SXI may be a useful tool for the mainland Chinese xerostomia patients for both clinical and epidemiologic researches. © Springer Science+Business Media Dordrecht 2013.


Yang Z.,Chongqing Medical University | Yang Z.,Chongqing Key Laboratory for Oral Diseases and Biomedical science | Ren L.,Chongqing Medical University | Deng F.,Chongqing Medical University | And 4 more authors.
Journal of Ultrasound in Medicine | Year: 2014

Objectives: Low-intensity pulsed ultrasound (US) can accelerate fracture healing and osteogenic differentiation. The aim of this study was to investigate the osteogenic effect of low-intensity pulsed US on human periodontal ligament cells and to determine whether bone morphogenetic protein (BMP)-Smad signaling was involved. Methods-Human periodontal ligament cells were exposed to low-intensity pulsed US at a frequency of 1.5 MHz and intensity of 90 mW/cm2 for 20 min/d. Osteogenic differentiation was determined by assaying alkaline phosphatase (ALP) and calcium deposition. Expression of BMP-2, BMP-6, and BMP-9 was detected by real-time polymerase chain reaction analysis. Phosphorylated Smad was detected by western blotting; Smad in the cells was labeled by an immunofluorescent antibody and observed by laserscanning confocal microscopy. Results: The optical density of ALP stimulated by US at 1.5 MHz and 90 mW/cm2 for 20 min/d was significantly higher than in other groups (P <.01); therefore, this dosage was considered optimal for promoting osteogenic differentiation. After 13 days of US exposure, ALP increased gradually after 5 days, peaked at 11 days, and decreased at 13 days, with a significant difference compared with the control group (P <.05). Osteocalcin production increased from 9 to 13 days and peaked at 15 days, with a significant difference compared with the control group (P <.05). BMP-2 and BMP-6 increased dynamically after exposure for 13 days. BMP-2 increased 6.07-fold at 3 days, 6.39-fold at 11 days, and 5.97-fold at 13 days. BMP-6 expression increased 6.82-fold at 1 day and 51.5-fold at 3 days and decreased thereafter. BMP-9 was not expressed. Phospho-Smad1/5/8 expression was significantly increased after exposure (P<.05) and transferred from the cytoplasm into the nuclei. Conclusions: Low-intensity pulsed US effectively induced osteogenic differentiation of human periodontal ligament cells, and the BMP-Smad signaling pathway was involved in the mechanism. © 2014 by the American Institute of Ultrasound in Medicine.


Li Y.,Chongqing Medical University | Li Y.,Chongqing Key Laboratory for Oral Diseases and Biomedical Science | Zhou Z.,Chongqing Medical University | Zhou Z.,Chongqing Key Laboratory for Oral Diseases and Biomedical Science
Journal of Pure and Applied Microbiology | Year: 2014

To construct LuxS deletion mutant of Streptococcus mutans, and study the effect of luxS mutation on the biofilm formation of streptococcus mutans under various conditions, and find out the differences between luxS mutant strain and streptococcus mutans. Long flanking homology polymerase chain reaction(LFH-PCR) was introduced to generate a gene disruption construct consisting of Emr cassette with long flanking homology regions to the target gene. The electroporation competence of Streptococcus mutans was then transformed with this PCR product. Then positive transformants were counted on selective agar which containing erythromycin and identified by PCR. The streptococcus mutans-luxS mutant and the standard strain were grown in three different conditions(BHI, 2% glucose-BHI, 2% saccharobiose-BHI), and the ability of S.mutans and LuxS mutant biofilm formation was examined in 24 h by scanning electron microscopy (SEM). Identification by PCR and sequencing confirmed the validity of the LuxS deletion mutant of Streptococcus mutans. Compared with S.mutans ,the LuxS mutant maintained with 2% sucrose displayed an apparent defect in biofilm formation. Conclusions: The successful construction of the LuxS deletion mutant, and the ability of sucrose-dependent biofilm formation will be down-regulated in Streptococcus mutans after LuxS gene was knocked out.


Deng J.-S.,Chongqing Medical University | Deng J.-S.,Chongqing Key Laboratory for Oral Diseases and Biomedical science | Qin P.,Chongqing Medical University | Li X.-X.,Chongqing Medical University | And 2 more authors.
Human Immunology | Year: 2013

The aim of this study was to perform a meta-analysis to evaluated the association between interleukin-1β (IL-1β) C(3953/4)T polymorphism and chronic periodontitis (CP). Systematic searches of electronic databases and hand searching of references were performed, including PubMed, Embase and Web of Science. The pooled odds ratios (ORs) with 95% confidence intervals (95%CIs) were calculated. Publication bias was tested by Begg's funnel plot and Egger's regression test. Sensitivity analysis was conducted by limiting the meta-analysis studies conforming to Hardy-Weinberg equilibrium (HWE) or high quality (score. ≥7). Data analyses were carried out by Stata 11.0. There were significant associations between IL-1β C(3953/4)T polymorphism and CP (for T allele vs. C allele: OR. =1.30, 95%CI. =1.05-1.60, p=0.02; for T/T vs. C/C: OR. =1.66, 95%CI. =1.12-2.45, p=0.01; for C/T. +. T/T vs. C/C: OR. =1.28, 95%CI. =0.99-1.65; and for T/T vs. C/T+C/C: OR. =1.62, 95%CI. =1.15-2.29, p=0.006). When stratified by ethnicity, statistically significantly elevated risk was found for Caucasians, but not for Asians. When stratified by study design, evidences of significant association was observed between IL-1β C(3953/4)T polymorphism and CP in both population-based studies and hospital-based studies. This meta-analysis indicates that there is strong evidence for association between IL-1β C(3953/4)T polymorphism and CP. © 2012 American Society for Histocompatibility and Immunogenetics.


Ye G.,Chongqing key Laboratory for Oral Diseases and Biomedical science | Ye G.,Chongqing Medical University | Li C.,Chongqing Medical University | Xiang X.,Chongqing key Laboratory for Oral Diseases and Biomedical science | And 13 more authors.
International Journal of Medical Sciences | Year: 2014

Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been found to have potent osteogenic activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected with recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of osteogenic markers such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) as well as mineralization ability were measured. The results showed that BMP9 promoted bone formation of PDLSCs. In other experiments, SB203580 and PD98059, which are inhibitors of p38 and ERK1/2, respectively, were used to determine if these kinases are involved in the osteogenic differentiation process. The resulting protein expression profiles and osteogenic markers of PDLSCs revealed that the mitogen-activated protein kinase (MAPK) signaling pathway might play an important role in the process of BMP9-induced osteogenic differentiation of PDLSCs. © Ivyspring International Publisher.


Wang J.-H.,Chongqing key Laboratory for Oral Diseases and Biomedical science | Wang J.-H.,Chongqing Medical University | Liu Y.-Z.,Chongqing Medical University | Liu Y.-Z.,Key Laboratory for Biochemistry and Molecular Pharmacology of Chongqing | And 16 more authors.
Bone | Year: 2013

Mesenchymal stem cells (MSCs) can self-renew and differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. It's reported that bone morphogenetic protein 9 (BMP9) is one of the most potent osteogenic BMPs to initiate the commitment of MSCs to osteoblast lineage. Cyclooxygenase-2 (COX-2) is critical for bone fracture healing and osteogenic differentiation in MSCs. However, the relationship between COX-2 and BMP9 in osteogenesis remains unknown. Herein, we investigate the role of COX-2 in BMP9-induced osteogenesis in MSCs. We demonstrate that COX-2 is up-regulated as a target of BMP9 in MSCs. Both COX-2 inhibitor (NS-398) and COX-2 knockdown siRNAs can effectively decrease alkaline phosphatase (ALP) activities induced by BMP9 in MSCs. NS-398 also down-regulates BMP9-induced expression of osteopontin and osteocalcin, so does the matrix mineralization. The in vivo studies indicate that knockdown of COX-2 attenuates BMP9-induced ectopic bone formation. In perinatal limb culture assay, NS-398 is shown to reduce the hypertropic chondrocyte zone and ossification induced by BMP9. Mechanistically, knockdown of COX-2 significantly inhibits the BMP9 up-regulated expression of Runx2 and Dlx-5 in MSCs, which can be rescued by exogenous expression of COX-2. Furthermore, knockdown of COX-2 apparently reduces BMP9 induced BMPR-Smad reporter activity, the phosphorylation of Smad1/5/8, and the expression of Smad6 and Smad7 in MSCs. NS-398 blocks the expression of BMP9 mediated by BMP9 recombinant adenovirus. Taken together, our findings suggest that COX-2 plays an important role in BMP9 induced osteogenic differentiation in MSCs; BMP9 and COX-2 may form an important regulatory loop to orchestrate the osteogenic differentiation in MSCs. © 2013 Elsevier Inc.


PubMed | Chongqing Key Laboratory for Oral Diseases and Biomedical science
Type: | Journal: Paediatric anaesthesia | Year: 2016

The incidence of early childhood caries shows a significant increasing trend. Often, children younger than 6 years need additional help to finish the dental treatment. Therefore, general anesthesia (GA) could help to provide a successful environment for pediatric dental treatment.The aim of this study was to assess the effect of dental treatment under general anesthesia (DGA) on the patients oral health in Chongqing, Southwest China using the P-CPQ and the FIS questionnaires.We collected the hospitals records of the children who received DGA from 2013 to 2014 and the questionnaires answered by their parents or caregivers before and after the treatment. Then the data were integrated and analyzed to assess the effect of DGA on the children and their family, the incidence of the complications during or after DGA, and the satisfaction of their parents.We collected 659 patients records including 55.4% boys and 44.6% girls. There were 443 children younger than 4 years, and 216 children older than 4 years. The main reason why they chose DGA was the patients fear for the treatment (95.1%). The mean cost of DGA almost reached a half (61.5%) or a third (15.3%) of the familys monthly earning. The mean P-CPQ score and FIS score significantly decreased after DGA. The highest incidence of complications was emergence agitation, headache, and nausea/vomiting. The incidence of emergence agitation and headache was related to the operation time. The majority of families reported a high degree of satisfaction.Childrens oral health-related quality of life after DGA improved significantly. Meanwhile DGA showed a positive effect on the whole family and majority of families reported a high degree of satisfaction to it.


PubMed | Chongqing Key Laboratory for Oral Diseases and Biomedical science
Type: Journal Article | Journal: Journal of oral rehabilitation | Year: 2013

The aim of this study was to investigate the psychometric properties of the Oro-facial Esthetic Scale among Chinese-speaking patients. The original Oro-facial Esthetic Scale was cross-culturally adapted in accordance with the international standards to develop a Chinese version (OES-C). Unlike the original Oro-facial Esthetic Scale, the version employed in this study used a 5-point Likert scale with items rated from unsatisfactory to most satisfactory. Psychometric evaluation included the reliability and validity of the OES-C. The reliability of the OES-C was determined through internal consistency and test-retest methods. The validity of OES-C was analysed by content validity, discriminative validity, construct validity and convergent validity. The corrected item-total correlation coefficients of the OES-C ranged from 0859 to 0910. The inter-item correlation coefficients between each two of the eight items of the OES-C ranged from 0766 to 0922. The values of ICC ranged from 079 (95% CI=054-098) to 093 (95% CI=087-099), indicating an excellent agreement. Construct validity was proved by the presence of one-factor structure that accounted for 83507% of the variance and fitted well into the model. Convergent validity was confirmed by the association between OES-C scores and self-reported oral aesthetics and three questions from the Oral Health Impact Profile related to aesthetics (correlation coefficients ranged from -0830 to -0702, P<0001). OES-C scores discriminated aesthetically impaired patients from healthy controls. This study provides preliminary evidence concerning the reliability and validity of the OES-C. The results show that the OES-C may be a useful tool for assessment of oro-facial esthetics in China.

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