Chongqing Institute of Traditional Chinese Medicine

Chongqing, China

Chongqing Institute of Traditional Chinese Medicine

Chongqing, China
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Qin Y.,Chongqing Institute of Traditional Chinese Medicine | Qin Y.,Taiji Group | Li Z.-W.,Chongqing Institute of Traditional Chinese Medicine | Li Z.-W.,Chongqing Medical University | And 8 more authors.
Journal of Drug Targeting | Year: 2014

Improving efficacy of inflammation treatment by increasing drug delivery to the inflammatory sites is a challenging endeavor. N-formyl-methionyl-leucyl- phenylalanine (fMLP), the first discovered leukocyte chemotaxis peptide, is composed of formyl methionine, leucine and phenylalanine. It conjugates with formyl peptide receptors on the target cells with high receptor expression on the surface such as macrophages. With this in mind, we developed a novel fMLP-modified liposome (fMLP-LIP) for enhancing drug delivery to the inflammatory sites and resolving the systemic reaction issue with conventional anti-inflammatory drugs. Being a more stable and cheaper liposomal component than phospholipids, cholesterol (CHO) has been thoroughly investigated as an alternative anchor. In this study, fMLP was covalently conjugated with CHO with polyethylene glycol link to prepare the liposomes, cellular uptake of liposomes by differentiated human U937 cells was examined and cellular uptake experiment in vitro was employed to optimize fMLP-LIP prescription and investigate the uptake mechanism. An in vivo inflammatory model was established to evaluate the targeting performance of fMLP-LIP to inflammatory site. The in vitro and in vivo findings indicate that the fMLP ligands playing an important role in increasing drug delivery to inflammatory sites and fMLP-LIP as a promising anti-inflammatory drug carrier. © 2014 Informa UK Ltd.


Yan X.,Chongqing Institute of Traditional Chinese Medicine | Yan X.,New Medical Science Research Institute | Shen H.,New Medical Science Research Institute | Jiang H.,Harvard University | And 4 more authors.
Cellular Physiology and Biochemistry | Year: 2013

Background/Aims: Colorectal cancer (CRC) is the second leading cause of cancer death in the Western countries. Novel approaches of treatment are needed for CRC. The purpose of the present study was to investigate cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human colorectal cancer cells. Methods: The effect of YXQ-EQ on viability, cell cycle progression and apoptosis in colorectal cancer HT-29 cells was investigated. Phosphorylation of Akt and Erk1/2, activation of NF-κB and the expression of proteins involved in regulation of cell cycle and apoptosis were examined by Western blot analysis. Results: YXQ-EQ markedly decreased viability and blocked colony formation of HT-29 cells. YXQ-EQ downregulated cyclin D1 expression and increased accumulation of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1, resulting in G1 cell cycle arrest. YXQ-EQ induced apoptosis in HT-29 cells in association with decreased expression of antiapoptotic proteins Bcl-xL, XIAP, survivin and Mcl-1 and elevated expression of proapoptotic protein Bax. YXQ-EQ significantly repressed phosphorylation of Akt and Erk1/2 and NF-κB activation in HT-29 cells, suggesting that YXQ-EQ may exert cytotoxic effect through regulating signaling pathways critical for cell proliferation and survival. Furthermore, YXQ-EQ treated PBS and an YXQ-EQ treated plant extract induced apoptosis in HT-29 cells. Conclusion: These findings show that YXQ-EQ has potent cytotoxic effect on HT-29 cells and suggest that YXQ-EQ could be potentially used for colorectal cancer treatment either directly or indirectly via carriers. Copyright © 2013 S. Karger AG, Basel.


Yan X.,Chongqing Institute of Traditional Chinese Medicine | Yan X.,New Medical Science Research Institute | Shen H.,New Medical Science Research Institute | Jiang H.,Harvard University | And 5 more authors.
Cellular Physiology and Biochemistry | Year: 2010

The antitumor effects of external Qi of Yan Xin Qigong (YXQ-EQ) have been widely described over the past three decades. To gain a better understanding of the mechanisms underlying YXQ-EQ's antitumor effects, in the present study we investigated its effects on growth, migration, invasion and apoptosis of breast cancer cells and the underlying molecular mechanisms. We show that YXQ-EQ treatment caused a time-dependent reduction in viability, blocked clonogenic growth and induced apoptosis in estrogen-independent breast cancer MDA-MB-231 cells. Furthermore, YXQ-EQ treatment blocked migration and invasion of MDA-MB-231 cells. Biochemically, YXQ-EQ treatment markedly inhibited constitutive and EGF-induced Akt phosphorylation. YXQ-EQ also substantially repressed NF-κB activity, resulting in decreased expression of anti-apoptotic Bcl-2, Bcl-XL, XIAP and survivin proteins. These findings suggest that YXQ-EQ may induce apoptosis and inhibition of migration and invasion of MDA-MB-231 cells through the repression of Akt/NF-κB signaling. Copyright © 2010 S. Karger AG, Basel.


Deng L.,Southwest University | Wang Q.,Southwest University | Wang Q.,Chongqing Institute of Traditional Chinese Medicine | Yuan H.,Southwest University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012

Objective: To study the effect of catalpol and puerarin freeze-dried powder for injection (CPFPI), a new compound traditional Chinese medicine(TCM) preparation, on coagulability, hemorheology and NO in rats with qi-deficiency and bloodstasis syndrome. Method: The model of rats with qi-deficiency and blood-stasis syndrome was established by hunger, fatigue, cold-dampness, panic and high fat diet. Coagulation time (CT) was observed by the glass method, and bleeding time (BT) was measured by tail-cutting method. The effects of CPFPI were also evaluated with prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). HCT was measured by the electric resistance method, hemorheology indicators were observed by auto-hemorheological instrument The level of NO in blood serum was measured by NO assay kit. Result: CPFPI 65.40 mg·kg -1 significantly prolonged CT, BT, PT, APTT and TT in rats. The viscosity of whole blood and plasma, hematocrit, erythrocyte aggregation and rigidity index, and reduced viscosity of whole blood in 65.40 mg·kg -1 groups were lower than model group. CPFPI 65.40 mg· kg -1 can raise the level of NO in blood serum. 32.70 mg·kg -1 markedly prolonged CT, PT and APTT and decreased whole blood viscosity , erythrocyte aggregation index and whole blood reduction viscosity. Conclusion: CPFPI has a significant effect in improving coagulability and hemorheology index and enhancing NO content in blood serum.


Li Z.-W.,Chongqing University | Huang H.,Chongqing University | Qin Y.,Taiji Group | Qin Y.,Chongqing Institute of Traditional Chinese Medicine | And 4 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2014

Objective To prepare and optimize the conditions of Sodium Aescinat micro-emulsion for injection. Methods Sodium Aescinat O/W micro-emulsion (soybean phospholipids + HS15/glycerin/medium chain triglycerides/water) was formulated guided by the pseudo-titration ternary phase diagram. Results Stable Sodium Aescinat micro-emulsion was obtained with medium chain triglycerides as oil, soybean phospholipids + HS15 (1 : 2) as emulsifier, glycerin as co-emulsifier, and a ratio of emulsifier and co-emulsifier (Km) between 2.00-3.75. Conclusion Stable Sodium Aescinat micro-emulsion can be prepared with conditions described in this study.


Cheng Y.-F.,Chongqing Institute of Traditional Chinese Medicine | Gou H.,Chongqing Institute of Traditional Chinese Medicine | Tan Q.-W.,Chongqing Institute of Traditional Chinese Medicine
Chinese Traditional and Herbal Drugs | Year: 2010

Objective Colla Corii Asini, Colla Carapacis et Plastri Testudinis, Colla Cornus Cervi, three types of plastic bead microwave process parameters were optimized in order to standardize the production of the types of plastic beads and to enhance the stability of the quality of plastic beads. Methods Using a number of quality indicators to evaluate the quality of plastic beads and orthogonal test to optimize the plastic bead process parameters. Results The microwave process parameters of Colla Corii Asini, Colla Carapacis et Plastri Testudinis, Colla Cornus Cervi, three types of plastic beads were obtained through orthogonal optimization. Conclusion Different types of plastic type drugs, different production lot, and different manufacturers of similar plastic herbs have their own optimum process parameters. L4 (23) orthogonal optimization can be used to screen out the plastic type drugs rapidly.


PubMed | Chongqing Institute of Traditional Chinese Medicine
Type: Journal Article | Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | Year: 2010

The antitumor effects of external Qi of Yan Xin Qigong (YXQ-EQ) have been widely described over the past three decades. To gain a better understanding of the mechanisms underlying YXQ-EQs antitumor effects, in the present study we investigated its effects on growth, migration, invasion and apoptosis of breast cancer cells and the underlying molecular mechanisms. We show that YXQ-EQ treatment caused a time-dependent reduction in viability, blocked clonogenic growth and induced apoptosis in estrogen-independent breast cancer MDA-MB-231 cells. Furthermore, YXQ-EQ treatment blocked migration and invasion of MDA-MB-231 cells. Biochemically, YXQ-EQ treatment markedly inhibited constitutive and EGF-induced Akt phosphorylation. YXQ-EQ also substantially repressed NF-kappaB activity, resulting in decreased expression of anti-apoptotic Bcl-2, Bcl-X(L), XIAP and survivin proteins. These findings suggest that YXQ-EQ may induce apoptosis and inhibition of migration and invasion of MDA-MB-231 cells through the repression of Akt/NF-kappaB signaling.


PubMed | Chongqing Institute of Traditional Chinese Medicine
Type: Journal Article | Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | Year: 2013

Colorectal cancer (CRC) is the second leading cause of cancer death in the Western countries. Novel approaches of treatment are needed for CRC. The purpose of the present study was to investigate cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human colorectal cancer cells.The effect of YXQ-EQ on viability, cell cycle progression and apoptosis in colorectal cancer HT-29 cells was investigated. Phosphorylation of Akt and Erk1/2, activation of NF-B and the expression of proteins involved in regulation of cell cycle and apoptosis were examined by Western blot analysis.YXQ-EQ markedly decreased viability and blocked colony formation of HT-29 cells. YXQ-EQ downregulated cyclin D1 expression and increased accumulation of cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip1), resulting in G1 cell cycle arrest. YXQ-EQ induced apoptosis in HT-29 cells in association with decreased expression of antiapoptotic proteins Bcl-xL, XIAP, survivin and Mcl-1 and elevated expression of proapoptotic protein Bax. YXQ-EQ significantly repressed phosphorylation of Akt and Erk1/2 and NF-B activation in HT-29 cells, suggesting that YXQ-EQ may exert cytotoxic effect through regulating signaling pathways critical for cell proliferation and survival. Furthermore, YXQ-EQ treated PBS and an YXQ-EQ treated plant extract induced apoptosis in HT-29 cells.These findings show that YXQ-EQ has potent cytotoxic effect on HT-29 cells and suggest that YXQ-EQ could be potentially used for colorectal cancer treatment either directly or indirectly via carriers.


PubMed | Chongqing Institute of Traditional Chinese Medicine
Type: Journal Article | Journal: Journal of drug targeting | Year: 2014

Improving efficacy of inflammation treatment by increasing drug delivery to the inflammatory sites is a challenging endeavor. N-formyl-methionyl-leucyl-phenylalanine (fMLP), the first discovered leukocyte chemotaxis peptide, is composed of formyl methionine, leucine and phenylalanine. It conjugates with formyl peptide receptors on the target cells with high receptor expression on the surface such as macrophages. With this in mind, we developed a novel fMLP-modified liposome (fMLP-LIP) for enhancing drug delivery to the inflammatory sites and resolving the systemic reaction issue with conventional anti-inflammatory drugs. Being a more stable and cheaper liposomal component than phospholipids, cholesterol (CHO) has been thoroughly investigated as an alternative anchor. In this study, fMLP was covalently conjugated with CHO with polyethylene glycol link to prepare the liposomes, cellular uptake of liposomes by differentiated human U937 cells was examined and cellular uptake experiment in vitro was employed to optimize fMLP-LIP prescription and investigate the uptake mechanism. An in vivo inflammatory model was established to evaluate the targeting performance of fMLP-LIP to inflammatory site. The in vitro and in vivo findings indicate that the fMLP ligands playing an important role in increasing drug delivery to inflammatory sites and fMLP-LIP as a promising anti-inflammatory drug carrier.


PubMed | Chongqing Institute of Traditional Chinese Medicine, Taiji Group, Chongqing Medical University and Chengdu University of Technology
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2014

To prepare and optimize the conditions of Sodium Aescinat micro-emulsion for injection.Sodium Aescinat O/W micro-emulsion (soybean phospholipids + HS15/glycerin/medium chain triglycerides/water) was formulated guided by the pseudo-titration ternary phase diagram.Stable Sodium Aescinat micro-emulsion was obtained with medium chain triglycerides as oil, soybean phospholipids + HS15 (1:2) as emulsifier, glycerin as co-emulsifier, and a ratio of emulsifier and co-emulsifier (Km) between 2.00-3.75.Stable Sodium Aescinat micro-emulsion can be prepared with conditions described in this study.

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