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Luo X.,Chongqing Medical University | Li L.,Chongqing Institute of Population and Family Planning | Ma M.,Chongqing Institute of Population and Family Planning | Li R.,Chongqing Institute of Population and Family Planning
Environmental Science and Pollution Research | Year: 2015

Cadmium (Cd) is an important toxic chemical due to its increasing levels in the environment and its resulting accumulation in humans and animals. The present study was performed to evaluate the long-term effects of low doses of Cd administered in offspring by oral route to rats during pregnancy and lactation. There were no adverse effects on the physical and sexual development in the pups, except to delay the development of offspring. The relative weights of livers and kidneys in the adult female offspring were significantly decreased after exposure to 10 ppm Cd. These results indicated that there were adverse effects on growth and development from exposure to 5 or 10 ppm Cd in utero and during lactation. The results also showed differential gender sensitivity effects on the organ weights. © 2015 Springer-Verlag Berlin Heidelberg


Li R.,Chongqing Institute of Population and Family Planning | Luo X.,Chongqing Medical University | Li L.,Chongqing Institute of Population and Family Planning | Peng Q.,Beibei District of Chongqing Municipal Public Security Bureau of Interpol Detachment | And 4 more authors.
Biological Trace Element Research | Year: 2015

Cadmium (Cd) is widely used in daily life and was recently recognized as a possible source of human toxicity due to its ability to accumulate in organs. Previous studies have shown that Cd exposure may cause testicular toxicity through oxidative stress and an inflammatory effect. Melatonin has been demonstrated to be an effective anti-oxidant and has an anti-inflammatory effect. The aim of the present study was to investigate the toxicological effects of Cd on reproduction in male mice and the potential protective action of melatonin against these adverse effects. Adult male mice were injected intraperitoneally with Cd at a dose of 2 mg/kg body weight per day for seven consecutive days with or without melatonin pretreatment. Sex organ weight, sperm parameters including sperm quality, apoptosis, acrosome integrity, mitochondrial membrane potential, testicular morphology, serum sex hormone, inflammatory status, and oxidative stress were evaluated. The results showed that significant adverse effects were observed in the male reproductive system after Cd exposure, including alterations in sperm parameters, increased DNA damage, and sex hormone disturbance. Acute Cd exposure also significantly increased malondialdehyde (MDA) contents, decreased glutathione (GSH) and superoxide dismutase (SOD) activities, and upregulated levels of the pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α), and interleukin-1beta (IL-1β), in the testis. In contrast, melatonin pretreatment significantly alleviated these toxic effects, and its mechanism may involve inhibiting MDA level, restoring GSH and SOD activities, and reducing the upregulation of TNF-α and IL-1β. Our data suggest that oxidative stress and inflammation are involved in Cd-induced toxicity in the male reproductive system and that co-administration of melatonin exerts a protective effect against Cd-induced male reproductive toxicity. © 2015 Springer Science+Business Media New York


Zhang D.,Key Laboratory of Birth Defects and Reproductive Health | Zhang D.,Chongqing Institute of Population and Family Planning | Li L.,Key Laboratory of Birth Defects and Reproductive Health | Li L.,Chongqing Institute of Population and Family Planning | And 15 more authors.
Gene | Year: 2013

Congenital heart disease (CHD) is the most frequently occurring congenital disorder in newborns and is the most frequent cause of infant death from birth defects. Human genetic studies have identified that numerous genes encoding transcription factors that regulate specific events in heart development are responsible for inherited and sporadic CHD. Nuclear factor-kappa B (NF-κB) is a major transcription regulator of immune response, apoptosis and cell-growth control genes. The aim of this study was to investigate whether the functional -94 insertion/deletion ATTG polymorphism (rs28362491) in the promoter of nuclear factor κB gene (NFKB1) is associated with susceptibility to CHD. Polymerase chain reaction (PCR)-polyacrylamide gel electrophoresis (PAGE) method was used to genotype rs28362491 in 122 atrial septal defect (ASD) patients, 114 ventricular septal defect (VSD) patients, and 412 controls. The frequencies of II (Insertion/Insertion) genotype in the ASD and VSD patients were significantly higher than that of controls (p= 0.004 for ASD Vs. controls, and p= 0.009 for VSD Vs. controls, respectively), and the frequencies for I allele in CHD patients were also significantly higher than that in controls (p= 0.01 for ASD Vs. controls, and p= 0.009 for VSD Vs. controls, respectively). This study suggests that the functional -94 insertion/deletion ATTG polymorphism in the promoter of NFKB1 is associated with CHD. © 2012 Elsevier B.V.

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