Chongqing Hifu Hospital

Chongqing, China

Chongqing Hifu Hospital

Chongqing, China

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Fu Z.,Chongqing Medical University | Wang L.,Chongqing HIFU Hospital | Ling L.,Chongqing Jiangbei District Peoples Hospital | Chen M.,Chongqing Yubei District Maternal and Child Health Care Hospital | And 4 more authors.
Chinese Journal of Interventional Imaging and Therapy | Year: 2016

Objective: To investigate the changes of local P16, Ki-67 and Fas expression in cervical intraepithelial neoplasia (CIN) grade I (CINI) before and after focused ultrasound treatment. Methods: Thirty patients with histologically confirmed CINI were treated with focused ultrasound and were followed up. All patients were examined for histopathological examination of the local cervical tissue after 3 months. The SP immunohistochemical method was used to detect the expression of P16, Ki-67 and Fas of cervical tissue before and after the treatment. Results: After focused ultrasound treatment, 25 patients were cured completely, 2 cases improved, the cervical returned to normal, 3 cases were not cured, and the total effective rate was 90.00% (27/30). The expression of P16, Ki-67 in local cervical tissue was significantly lower than that before treatment, but the expression of Fas was up-regulated (all P<0.05). Conclusion: The focused ultrasound can treat CINI effectively by reducing the expression of P16 and Ki-67 and increasing the expression of Fas in local cervical tissues to regulate cell proliferation and apoptosis, and to prevent it to the high grade. Copyright © 2016 by the Chinese Medical Association.


Shao R.,Chongqing Medical University | Xing R.,Chongqing Medical University | Xiong Y.,Chongqing Hifu Hospital | Fang L.,Chongqing Medical University | Wang Z.,Chongqing Medical University
Biochemical and Biophysical Research Communications | Year: 2015

Uterine leiomyomas are benign myometrial neoplasms that function as one of the common indications for hysterectomy. Clinical and biological evidences indicate that uterine leiomyomas are estrogen-dependent. Estrogen stimulates cell proliferation through binding to the estrogen receptor (ER), of which both subtypes α and β are present in leiomyomas. Clinically, leiomyomas may be singular or multiple, where the first one is rarely recurring if removed and the latter associated to a relatively young age or genetic predisposition. These markedly different clinical phenotypes indicate that there may different mechanism causing a similar smooth muscle response. To investigate the relative expression of ERα and ERβ in multiple and solitary uterine leiomyomas, we collected samples from 35 Chinese women (multiple leiomyomas n = 20, solitary leiomyoma n = 15) undergoing surgery to remove uterine leiomyomas. ELISA assay was performed to detect estrogen(E2) concentration. Quantitative real-time PCR analysis was performed to detect ERα and ERβ mRNA expression. Western blot and immunohistochemical analysis were performed to detect ERα and ERβ protein expression. We found that ERα mRNA and protein levels of in multiple leiomyomas were significantly lower than those of solitary leiomyomas, whereas ERβ mRNA and protein levels in multiple leiomyomas were significantly higher than those in solitary leiomyomas, irrespectively of the menstrual cycle stage. In both multiple and solitary leiomyomas, ERα expression was higher than that of ERβ. E2 concentration in multiple and solitary leiomyomas correlated with that of ERα expression. ERα was present in nuclus and cytoplasma while estrogen receptor β localized only in nuclei in both multiple and solitary leiomyomas. Our findings suggest that the difference of ERα and ERβ expression between multiple and solitary leiomyomas may be responsible for the course of the disease subtypes. © 2015 Elsevier Inc.


Long L.,Chongqing Medical University | Chen J.,Chongqing Medical University | Xiong Y.,Chongqing HIFU Hospital | Zou M.,Chongqing HIFU Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: The aim of this study was to evaluate the efficacy, safety and sexual life quality outcomes of ultrasound-guided high-intensity focused ultrasound (HIFU) ablations for the treatment of patients with symptomatic adenomyosis and uterine volumes >200 cm3. Methods: In our prospective clinical trial 47 patients with uterine volumes >200 cm3 and symptomatic adenomyosis were treated with single treatment sessions of ultrasound-guided HIFU ablations. Beside uterus and adenomyosis lesion size reductions, outcome measures were symptom severity score (SSS), visual analogue scale (VAS) of dysmenorrhea, female sexual function index (FSFI) scores and the incidence of complications. Results: In all 47 patients, the adenomyosis lesions sizes were significantly reduced 12 months after the interventions (P<0.01). The SSS and dysmenorrhea VAS scores were significantly reduced 12 months after the interventions (P<0.01) and the FSFI scores gradually improved during 12 months after the HIFU ablations (P<0.001). No serious complication occurred. Conclusion: Ultrasound-guided HIFU ablation is a safe and effective noninvasive alternative for the treatment of uterine volumes >200 cm3 with symptomatic adenomyosis. Particularly maintaining the integrity of patients’ uteri leads to significant FSFI score improvements, which were essentially reduced before the HIFU treatments. © 2015, E-Century Publishing Corporation. All rights reserved.


PubMed | Chongqing Medical University and Chongqing Hifu Hospital
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2015

Uterine leiomyomas are benign myometrial neoplasms that function as one of the common indications for hysterectomy. Clinical and biological evidences indicate that uterine leiomyomas are estrogen-dependent. Estrogen stimulates cell proliferation through binding to the estrogen receptor (ER), of which both subtypes and are present in leiomyomas. Clinically, leiomyomas may be singular or multiple, where the first one is rarely recurring if removed and the latter associated to a relatively young age or genetic predisposition. These markedly different clinical phenotypes indicate that there may different mechanism causing a similar smooth muscle response. To investigate the relative expression of ER and ER in multiple and solitary uterine leiomyomas, we collected samples from 35 Chinese women (multiple leiomyomas n=20, solitary leiomyoma n=15) undergoing surgery to remove uterine leiomyomas. ELISA assay was performed to detect estrogen(E2) concentration. Quantitative real-time PCR analysis was performed to detect ER and ER mRNA expression. Western blot and immunohistochemical analysis were performed to detect ER and ER protein expression. We found that ER mRNA and protein levels of in multiple leiomyomas were significantly lower than those of solitary leiomyomas, whereas ER mRNA and protein levels in multiple leiomyomas were significantly higher than those in solitary leiomyomas, irrespectively of the menstrual cycle stage. In both multiple and solitary leiomyomas, ER expression was higher than that of ER. E2 concentration in multiple and solitary leiomyomas correlated with that of ER expression. ER was present in nuclus and cytoplasma while estrogen receptor localized only in nuclei in both multiple and solitary leiomyomas. Our findings suggest that the difference of ER and ER expression between multiple and solitary leiomyomas may be responsible for the course of the disease subtypes.

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