Chongqing Medal Eye Institute

Chongqing, China

Chongqing Medal Eye Institute

Chongqing, China
SEARCH FILTERS
Time filter
Source Type

Li M.,Chongqing Medical University | Du Z.,Chongqing Medical University | Wang M.,Chongqing Medical University | Yan P.,Chongqing Medal Eye Institute | And 2 more authors.
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2017

Background: High intensity focused ultrasound (HIFU) is a novel noninvasive technique and it has been used to clinical treatment of solid tumors. However, whether it is safe to cure eye related diseases has not been reported. Objective: This study was to explore the influence of HIFU on retinas of living rabbits after the irradiation on cornea, and provide a theoretical basis for the safety of clinical therapy. Methods: Forty-one rabbits were involved. The corneal stroma of the right eye was irradiated by HIFU that was fixed in circular shape. The left eye with no treatment served as control group. Six rabbits were sacrificed at day 1, 7, 14, 30, 60 and 90 days after irradiation, respectively. The eyeballs of 3 rabbits were collected for paraffin imbedding. Histological method was then used to evaluate the structure of retina. Immunohistochemistry was used to determine the expression of Nogo-A.TUNEL method was carried out to examine cell apoptosis of different layers of retina. The retina of the rest 3 rabbits were collected to determine the contents of superoxide dismutase (SOD) and malondialdehyde (MDA). The retinal morphology of 5 rabbits were scanned by OCT and retinal thickness was recorded before irradiation and 1, 7, 14, 30, 60 and 90 days after irradiation. The use and care of experimental animals complied with Regulations on the Administration of Experimental Animals from National Ministry of Science and Technology. Results: The number of retinal cell layers remained intact in the cornea of living rabbits after HIFU irradiation. The structure of each layer was densely and orderly arranged with clear borders between layers. The histological morphology of retinal tissues were the same among control eyes and experimental eyes at different time points. There was no significant difference in Nogo-A expression among control eyes and experimental eyes at different time points (Fgroup=0.672, P=0.413; Ftime=1.622, P=0.157). No significant difference was obtained in SOD activity and MDA contents among control eyes and experimental eyes at different time points (SOD:Fgroup=0.167, P=0.684; Ftime=0.074, P=0.996.MDA: Fgroup=0.005, P=0.946; Ftime=0.405, P=0.844). No TUNEL positive cell was obtained in each group. The thickness of retinal tissues showed no significant difference among before irradiation and 1, 7, 14, 30, 60 and 90 days after irradiation (F=0.150, P=0.988). Conclusions: The irradiation of HIFU on the cornea of living rabbits shows no significant influence on the morphology and bioactive factors of retina. Therefore, the therapeutic application of HIFU irradiation on cornea is safe for retina. Copyright © 2017 by the Chinese Medical Association.


Zhang Y.,Chongqing Medal Eye Institute | Yan P.-S.,Chongqing Medal Eye Institute
Chinese Journal of Ophthalmology | Year: 2013

Objective: To assess the quality of vision in the early stage after sub-bowman keratomileusis (SBK) for the correction of moderate and high myopia. Methods: Retrospective case series. Sixty eight myopic eyes of 35 consecutive patients in Chongqing Medal Eye Institute from May to November 2011 were enrolled in this study. All patients were evaluated at 2 weeks, 1 and 3 months postoperatively. The main measurements included visual acuity, corneal and total optical wavefront aberration, photopic and mesopic contrast sensitivity. Results: The median values of preoperative uncorrected central visual acuity (UCVA) and best corrected visual acuity (BCVA) were 0.05 and 1.00, respectively. The median value of postoperative UCVA was 1.20. There was no significant difference between the preoperative and postoperative anterior corneal total aberration (F = 0.712, P > 0.05). Compared with preoperative value (0.47 ± 0.17) μm, the corneal high orders aberrations in 2 weeks after SBK (1.37 ± 0.72) μm increased by 3 times. The total optical aberrations in 2 weeks after SBK (0.99 ± 0.58) μm decreased to one-eighth of preoperative measurements (8.26 ± 2.93) μm. Compared with preoperative value (0.30 ± 0.12) μm, the total high order aberrations in 2 weeks after SBK (0.46 ± 0.19) μm increased by 1.5 times. Although photopic and mesopic contrast sensitivity fluctuated mildly after SBK, the measurements did not decline significantly. Compared with preoperative data, mesopic contrast sensitivity at 3 months increased 0.294 (F = 20.88, P < 0.01) and 0.296 (F = 13.64, P < 0.01) at 12 and 18 c/d, respectively. Conclusion: Patients could obtain good quality of vision at the early stage after SBK. Copyright © 2013 by the Chinese Medical Association.


PubMed | Chongqing Medal Eye Institute
Type: Journal Article | Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology | Year: 2013

To assess the quality of vision in the early stage after sub-bowman keratomileusis (SBK) for the correction of moderate and high myopia.Retrospective case series. Sixty eight myopic eyes of 35 consecutive patients in Chongqing Medal Eye Institute from May to November 2011 were enrolled in this study. All patients were evaluated at 2 weeks, 1 and 3 months postoperatively. The main measurements included visual acuity, corneal and total optical wavefront aberration, photopic and mesopic contrast sensitivity.The median values of preoperative uncorrected central visual acuity (UCVA) and best corrected visual acuity (BCVA) were 0.05 and 1.00, respectively. The median value of postoperative UCVA was 1.20. There was no significant difference between the preoperative and postoperative anterior corneal total aberration (F = 0.712, P > 0.05). Compared with preoperative value (0.47 0.17)m, the corneal high orders aberrations in 2 weeks after SBK (1.37 0.72) m increased by 3 times. The total optical aberrations in 2 weeks after SBK (0.99 0.58) m decreased to one-eighth of preoperative measurements (8.26 2.93) m. Compared with preoperative value (0.30 0.12)m, the total high order aberrations in 2 weeks after SBK (0.46 0.19) m increased by 1.5 times. Although photopic and mesopic contrast sensitivity fluctuated mildly after SBK, the measurements did not decline significantly. Compared with preoperative data, mesopic contrast sensitivity at 3 months increased 0.294 (F = 20.88,P < 0.01) and 0.296 (F = 13.64, P < 0.01) at 12 and 18 c/d, respectively.Patients could obtain good quality of vision at the early stage after SBK.

Loading Chongqing Medal Eye Institute collaborators
Loading Chongqing Medal Eye Institute collaborators