Chongqing Engineering Technology Research Center for Import and Export Food Safety

Chongqing, China

Chongqing Engineering Technology Research Center for Import and Export Food Safety

Chongqing, China
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Wang G.,Chongqing Engineering Technology Research Center for Import and Export Food Safety | Zhao J.,Centers for Disease Control and Prevention | Peng T.,Chinese Academy of Inspection and Quarantine | Chen D.,Chinese Academy of Inspection and Quarantine | And 3 more authors.
Journal of Separation Science | Year: 2013

Matrix effects in determination of three β-receptor agonists including salbutamol (SAL), clenbuterol, and terbutaline in animal-derived foodstuffs were studied by ultra-performance LC-MS/MS with cleanup of immunoaffinity SPE column (IAC). Some animal tissue samples including pig liver, swine muscle, and fish muscle were hydrolyzed by the mixed enzyme solution or HCl solution, and the cleanup efficiencies with SAL IAC, MCX SPE column, and C18-SCX tandem columns were examined and compared by using spiked experiments. The results showed that the matrix effects in the determination of SAL and terbutaline can be eliminated with SAL IAC cleanup, and the average recoveries of SAL were 77.4∼81.5%, 79.0∼80.3%, and 85.0∼87.2% in pig liver, swine muscle, and fish muscle, respectively. The decision limit (ccα) and detection capability (ccβ) for SAL in pig liver were 0.02 and 0.05 μg/kg, respectively. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Zou Y.,Southwest University | Zhao J.,Centers for Disease Control and Prevention | Zhang J.,Southwest University | Wang G.,Chongqing Engineering Technology Research Center for Import and Export Food Safety | And 3 more authors.
Analytical Methods | Year: 2013

Animal tissue samples were extracted with ethyl acetate or alkalized ethyl acetate, and purified with MCX or LC-18 solid-phase extraction columns, and the matrix effects (ME%) in the simultaneous determination of four fenicol antibiotics including chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) were studied by ultra performance liquid chromatography-tandem mass spectrometry. ME% for FFA in swine muscle and casings extracted by ethyl acetate with LC-18 column cleanup were 11.1% and 10.4%, and in swine muscle extracted by ethyl acetate and alkalized ethyl acetate with MCX column cleanup ME% were 8.1% and 16.1%, respectively, showing strong signal suppression; while ME% for FF in swine muscle extracted by ethyl acetate and alkalized ethyl acetate with LC-18 column cleanup were 148.5% and 146.9%, and in swine muscle extracted by ethyl acetate and alkalized ethyl acetate with MCX column cleanup were 234.3% and 150.9%, showing strong signal reinforcement. Deuterated chloramphenicol-d5 could only be used as an internal standard to eliminate matrix effects for CAP determination. It may be that the most effective method to eliminate matrix interference in the simultaneous determination of the four analytes is quantified by using matrix-matched solutions. Under the optimum conditions of sample pretreatment and quantitative analysis, the decision limits (ccα as specified in European Directive 2002/657/EC) for FFA, TAP, CAP and FF in swine muscle were determined to be 0.4, 0.05, 0.02 and 0.02 μg kg-1, and 0.5, 0.1, 0.04 and 0.03 μg kg-1 in casings; the detection capabilities in swine muscle were determined to be 0.7, 0.09, 0.05 and 0.05 μg kg-1, and 1.2, 0.19, 0.12 and 0.07 μg kg-1 in casings, respectively. © 2013 The Royal Society of Chemistry.


PubMed | Chongqing Engineering Technology Research Center for Import and Export Food Safety
Type: Evaluation Studies | Journal: Journal of separation science | Year: 2013

Matrix effects in determination of three -receptor agonists including salbutamol (SAL), clenbuterol, and terbutaline in animal-derived foodstuffs were studied by ultra-performance LC-MS/MS with cleanup of immunoaffinity SPE column (IAC). Some animal tissue samples including pig liver, swine muscle, and fish muscle were hydrolyzed by the mixed enzyme solution or HCl solution, and the cleanup efficiencies with SAL IAC, MCX SPE column, and C(18) -SCX tandem columns were examined and compared by using spiked experiments. The results showed that the matrix effects in the determination of SAL and terbutaline can be eliminated with SAL IAC cleanup, and the average recoveries of SAL were 77.4~81.5%, 79.0~80.3%, and 85.0~87.2% in pig liver, swine muscle, and fish muscle, respectively. The decision limit (cc) and detection capability (cc) for SAL in pig liver were 0.02 and 0.05 g/kg, respectively.

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