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Wang B.,Southwest University | Wang B.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Wang B.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Wu Y.,Southwest University | And 11 more authors.
Sensors and Actuators, B: Chemical | Year: 2017

A flexible free-standing paper sensor was fabricated for the first time via in situ growth of Cu nanoflower on reduced graphene oxide (RGO) sheets towards amperometrically non-enzymatic detection of glucose. The low cost of copper (Cu) and graphene made the prepared sensor inexpensive in comparison with sensors made from noble metal catalysts. This Cu-RGO paper sensor exhibited high sensitivity and good selectivity when utilised in glucose sensing attributed to the synergistic effects of graphene paper and Cu nanoflowers. The combination of excellent catalytic activity, largely reactive surface area of Cu nanoflower and good conductivity of graphene paper enabled the sensor to perform well in glucose detection. The prepared glucose sensor holds a great promise for inexpensive flexible electrochemical non-enzymatic glucose sensors. © 2016 Elsevier B.V.


Lu Z.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Lu Z.,Southwest University | Lu Z.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Mao C.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | And 5 more authors.
Journal of Materials Chemistry C | Year: 2015

A repeated coating-reduction approach was developed to directly immobilize graphene nanosheets on silk for high conductivity. The as-prepared highly conductive graphene-coated silk fabrics (1.5 kΩ sq-1) and fibers (3595 S m-1) are promising as the functional supporting matrix and conducting fabrics/wires in future wearable electronics. This journal is © The Royal Society of Chemistry 2015.


Yu L.,Southwest University | Yu L.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Yu L.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Shi Z.Z.,Southwest University | And 2 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2015

Microfluidic paper-based analytical devices (μPADs) attract tremendous attention as an economical tool for in-field diagnosis, food safety and environmental monitoring. We innovatively fabricated 2D and 3D μPADs by photolithography-patterning microchannels on a Parafilm® and subsequently embossing them to paper. This truly low-cost, wax printer and cutter plotter independent approach offers the opportunity for researchers from resource-limited laboratories to work on paper-based analytical devices. © The Royal Society of Chemistry 2015.


Lu Z.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Lu Z.,Southwest University | Lu Z.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Wang J.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | And 12 more authors.
Chemical Communications | Year: 2015

Transient spikes from the bacteriorhodopsin (bR) photocycle are triggered with NIR irradiation for the first time by integrating bR with upconversion nanoparticles. This work may open new horizons for the bR applications in the IR wavelength range. This journal is © The Royal Society of Chemistry 2015.


Hu W.,Southwest University | Hu W.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Hu W.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Liu Y.,Southwest University | And 7 more authors.
Advanced Materials | Year: 2015

(Figure Presented). A hierarchically nanostructured organic-inorganic hybrid substrate comprising randomly oriented ZnO nanorods on glass slide with coaxially tethered dense polymer brush, POEGMAcoGMA is reported for highly sensitive antibody microassay, achieving excellent detection specificity, and superior detection limit of as low as 100 fg mL-1 for biomarkers in human serum within a 1 h assay time. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA,.


Wang B.,Southwest University | Wang B.,Wuhan University | Wang B.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Wang B.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | And 19 more authors.
Biosensors and Bioelectronics | Year: 2016

Novel fluorescent nitrogen-doped carbon dots (N,C-dots) were synthesized and assembled on aptamer modified gold nanoparticles (Aptamer/AuNPs) for the super sensitive detection of aflatoxin B1 (AFB1). Positively charged N,C-dots were synthesized by the hydrothermal treatment of pancreatin. The prepared N,C-dots were assembled on aptamer/AuNPs by electrostatic interactions. The fluorescence of the N,C-dots was efficiently quenched. When AFB1 was added to the assay solution, specific interactions between AFB1 and the aptamer caused release of the N,C-dots. The fluorescence of the N,C-dots recovered and the intensity increase could be used to calculate the amount of AFB1 added. The assay exhibits super-high sensitivity with a detection limit of 5pg/mL (16pM) and a wide range of linear response of 5pg/mL to 2.00ng/mL. A novel aptasensor is thus successfully constructed, it provides an efficient way for sensitive AFB1 sensing as well as a new technique for aptamer based novel sensor construction. © 2015 Elsevier B.V.


Li M.,Southwest University | Li M.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Shi Z.Z.,Southwest University | Shi Z.Z.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | And 7 more authors.
Analytica Chimica Acta | Year: 2016

The complement fixation test (CFT) is a serological test that can be used to detect the presence of specific antibodies or antigens to diagnose infections, particularly diseases caused by microbes that are not easily detected by standard culture methods. We report here, for the first time, a poly(dimethylsiloxane) (PDMS)/glass slide hybrid microfluidic device that was used to manipulate the solution compartment and communication within the microchannel to establish sampler and indicator systems of CFT. Two types of on-chip CFT, solution-based and solid phase agar-based assays, were successfully demonstrated for biomarker carcinoembryonic antigen (CEA) and recombinant avian influenza A (rH7N9) virus protein detection. In addition, the feasibility of the on-chip CFT in assaying real biopsy was successfully demonstrated by specifically detecting rH7N9 and CEA in human serum. The results demonstrated that the miniaturized assay format significantly reduced the assay time and sample consumption. Exemption from protein immobilization, blocking, complicated washing steps and expensive enzyme/fluorescein conjugates highlights the merits of on-chip CFT over ELISA. Most attractively, the on-chip agar-based CFT results can be imaged and analysed by smartphone, strengthening its point-of-care application potential. We anticipate that the on-chip CFT reported herein will be a useful supplemental or back-up tool for on-chip immunoassays such as ELISA for disease diagnosis and food inspection. © 2016 Elsevier B.V.


Lu Z.,Southwest University | Lu Z.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | Chen X.,Southwest University | Chen X.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | And 6 more authors.
Microchimica Acta | Year: 2014

Aflatoxin B1 (AFB1), a secondary fungal metabolite of Aspergillus flavus, was employed as a model mycotoxin to establish an aptamer based assay that exploits the quenching of the fluorescence of CdTe quantum dots (Q-dots) by graphene oxide (GO). A thiolated aptamer specific for AFB1 was linked to the surface of Q-dots via ligand exchange. The fluorescence of the aptamer modified-Q-dots is strongly quenched by GO. If, however, AFB1 is added, fluorescence is restored depending on the quantity of AFB1 added. The system was evaluated both in phosphate buffer solution and in peanut oil. If performed in an aqueous system, the assay possesses good selectivity, a wide dynamic range (from 3.2 nM to 320 μM) and a low limit of detection (1.0 nM). If performed in peanut oil solution, the dynamic range is from 1.6 nM to 160 μM, and the limit of detection is 1.4 nM. In our perception, this is a simple, sensitive and selective method for the determination of AFB1 that also may be extended to the analysis of other mycotoxins. © 2014, Springer-Verlag Wien.


Lu Z.,Southwest University | Lu Z.,Chongqing Key Laboratory for Advanced Materials and oTechnologies of Clean Energies | Lu Z.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Mao C.,Southwest University | And 20 more authors.
Journal of Colloid and Interface Science | Year: 2014

To endow silk with UV-shielding ability and antibacterial activity, CeO2 nanoparticles were immobilized on silk surface via a dip-coating approach without changing silk structure. Surface density of the nanoparticles could be easily adjusted by controlling the number of dip-coating cycle. Enhanced thermal stability of the modified silk is exhibited in thermogravimetric analysis (TGA) and derivative thermogravimetric analysis (DTG). The excellent UV-protection ability and antibacterial property of the CeO2 nanoparticle-coated silk are demonstrated in UV-vis diffuse reflectance spectroscopy and colony-forming capability test, respectively. Based on the data, it can be concluded that CeO2 nanoparticles could be used as a very promising coating material to modify silk for UV-protection and antibacterial applications. © 2014 Elsevier Inc.


Chen H.,Southwest University | Chen H.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | Hu W.,Southwest University | Hu W.,Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases | And 2 more authors.
Sensors and Actuators, B: Chemical | Year: 2015

Heavy metal mercury(II) (Hg2+) ion is a highly toxic pollution widely distributed in air, water and soil. Simple and reliable on-site detection of Hg2+ has raised considerable concerns for environmental protection and human health but current methods necessitate sophisticated instruments and/or tedious procedures. In this work we report a simple, reliable and instrument-free colorimetric method for Hg2+ detection based on its inhibition effect on 2,2′-Bipyridyl (Bipy) induced aggregation of gold nanoparticles (AuNPs). Bipy is found to be capable of triggering the quasi-linear aggregation of citrate-capped monodisperse AuNPs with a controllable manner and generate visible color change; in the presence of Hg2+ the aggregation is efficiently decelerated/inhibited, allowing naked-eye quantification of Hg2+ in a range from 0.2 to 2.0 μM. The detection limit is further down to 38 nM when coupled to a simple UV-vis spectrometer. The method shows good selectivity toward Hg2+ and excellent reliability in various practical samples with different pH values or ionic strengths, thus holding great promise for rapid and on-site detection of Hg2+. © 2015 Elsevier B.V. All rights reserved.

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