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Yu L.,Southwest University | Yu L.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Yu L.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | Shi Z.Z.,Southwest University | And 2 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2015

Microfluidic paper-based analytical devices (μPADs) attract tremendous attention as an economical tool for in-field diagnosis, food safety and environmental monitoring. We innovatively fabricated 2D and 3D μPADs by photolithography-patterning microchannels on a Parafilm® and subsequently embossing them to paper. This truly low-cost, wax printer and cutter plotter independent approach offers the opportunity for researchers from resource-limited laboratories to work on paper-based analytical devices. © The Royal Society of Chemistry 2015.


Hu W.,Southwest University | Hu W.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Hu W.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | Liu Y.,Southwest University | And 7 more authors.
Advanced Materials | Year: 2015

(Figure Presented). A hierarchically nanostructured organic-inorganic hybrid substrate comprising randomly oriented ZnO nanorods on glass slide with coaxially tethered dense polymer brush, POEGMAcoGMA is reported for highly sensitive antibody microassay, achieving excellent detection specificity, and superior detection limit of as low as 100 fg mL-1 for biomarkers in human serum within a 1 h assay time. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA,.


Wang B.,Southwest University | Wang B.,Wuhan University | Wang B.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Wang B.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | And 19 more authors.
Biosensors and Bioelectronics | Year: 2016

Novel fluorescent nitrogen-doped carbon dots (N,C-dots) were synthesized and assembled on aptamer modified gold nanoparticles (Aptamer/AuNPs) for the super sensitive detection of aflatoxin B1 (AFB1). Positively charged N,C-dots were synthesized by the hydrothermal treatment of pancreatin. The prepared N,C-dots were assembled on aptamer/AuNPs by electrostatic interactions. The fluorescence of the N,C-dots was efficiently quenched. When AFB1 was added to the assay solution, specific interactions between AFB1 and the aptamer caused release of the N,C-dots. The fluorescence of the N,C-dots recovered and the intensity increase could be used to calculate the amount of AFB1 added. The assay exhibits super-high sensitivity with a detection limit of 5pg/mL (16pM) and a wide range of linear response of 5pg/mL to 2.00ng/mL. A novel aptasensor is thus successfully constructed, it provides an efficient way for sensitive AFB1 sensing as well as a new technique for aptamer based novel sensor construction. © 2015 Elsevier B.V.


Lu Z.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Lu Z.,Southwest University | Lu Z.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | Mao C.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | And 5 more authors.
Journal of Materials Chemistry C | Year: 2015

A repeated coating-reduction approach was developed to directly immobilize graphene nanosheets on silk for high conductivity. The as-prepared highly conductive graphene-coated silk fabrics (1.5 kΩ sq-1) and fibers (3595 S m-1) are promising as the functional supporting matrix and conducting fabrics/wires in future wearable electronics. This journal is © The Royal Society of Chemistry 2015.


Yu L.,Southwest University | Yu L.,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies | Yu L.,Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases | Shi Z.,Southwest University | And 8 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

In vitro cell-based analysis is strongly affected by material's surface chemical properties. The cell spreading, migration, and proliferation on a substrate surface are initiated and controlled by successful adhesion, particularly for anchor-dependent cells. Unfortunately, polydimethylsiloxane (PDMS), one of the most used polymeric materials for construction of microfluidic and miniaturized biomedical analytic devices, is not a cell-friendly surface because of its inherent hydrophobic property. Herein, a poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] (poly(GMA-co-pEGMA)) polymer brush was synthesized on a PDMS surface through a surface-initiated atom-transfer radical polymerization method. Contact angle and Fourier transform infrared characterization show that the poly (GMA-co-pEGMA) polymer brush functionalization can increase wettability of PDMS and introduce epoxy, hydroxyl, and ether groups into PDMS surface. In vitro cell growth assay demonstrates that cell adhesion and proliferation on poly(GMA-co-pEGMA) polymer brush-functionalized PDMS (poly(GMA-co-pEGMA)@PDMS) are better than on pristine PDMS. Additionally, immobilization of collagen type I (CI) and fibronectin (FN) on poly(GMA-co-pEGMA)@PDMS is better than direct coating of CI and FN on pristine PDMS to promote cell adhesion. Furthermore, increased intracellular reactive oxygen species and cell mitochondrial membrane depolarization, two indicators of cell oxidative stress, are observed from cells growing on pristine PDMS, but not from those on poly(GMA-co-pEGMA)@PDMS. Collectively, we demonstrate that poly(GMA-co-pEGMA) functionalization can enhance cell adhesion and proliferation on PDMS, and thus can be potentially used for microfluidic cell assay devices for cellular physiology study or drug screening. © 2015 Wiley Periodicals, Inc.

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