Cai L.,Chongqing Academy of Chinese Materia Medica |
Cai L.,Beijing University of Chinese Medicine |
Zhong G.,Chongqing Academy of Chinese Materia Medica |
Zhong G.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
And 4 more authors.
Zhongguo Zhongyao Zazhi
Objective: A HPLC method was established for the determination of 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside and anthraquinon simultaneously in Polygonum multiflori. The contents variation from different growing stages in P. multifield were observed. Method: The HPLC method was used to measure the contents of 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D- glucopyranoside and anthraquinon simultaneously in 1-4 years old P. multiflori which were harvested in July to December. Chromatographic conditions: The chromatography column was C18 with a mobile phase composed of methanol-0.1% water phosphoric acid. The detection wavelength was 320 nm and 254 nm respectively, and the flow rate was 1.0 mL ·min-1. Result: In a certain range, 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D- glucopyranoside, emodin and physcion showed good linearity, precision, reproducibility, stability and average recovery. Conclusion: The developed method is sensitive, accurate. The contents of 2, 3, 5, 4′- tetrahydroxystilbene-2-O-β-D-glucopyranoside and anthraquinon in P. multiflorum rapidly accumulated in one to two years, and in three year that was the highest. The samples collected in November accumulate much higher amounts of 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside and anthraquinon than those which were collected in other months. Source
Wu Y.,Chongqing Academy of Chinese Materia Medica |
Wu Y.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
Li L.,Chongqing Academy of Chinese Materia Medica |
Li L.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
And 6 more authors.
Zhongguo Zhongyao Zazhi
Objective:To explore the effects of micro-elements fertilizers on the quality and yields of Artemisia annua. Method:Field experiments were conducted according to the method of random blocks design. After the harvest the yield was calculated and the content of artemisinin was determined. Result:By applying 0.1%-0.5% Mn and 0.1%-0.5% Zn the dried leaf output and artemisinin content were increased. Conclusion:The suitable ranges of Mn and Zn can increased the yield and artemisinin content of A. annua. Source
Wu Y.-K.,Chongqing Academy of Chinese Materia Medica |
Wu Y.-K.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
Wu Y.-K.,Southwest University |
Liu X.,Chongqing Academy of Chinese Materia Medica |
And 7 more authors.
Zhongguo Zhongyao Zazhi
The interspecific association of companion species in Artemisia annua community in 48 region of southwest China was analyzed by variance analysis, χ2-test and association indices. The total related variance ratio among species in A. annua community was 2.05. Among 45 species pairs of 10 main species in the community, only 4 pairs showed significant negative correlations. χ2 test, PC, OI, DI and AC values indicated pairs 1-8 (A. annua-A. lactiflora), 1-9 (A. annua-Setaria viridis) and 1-10 (A. annua-Bidens pilosa) showed a high correlations, and common utilization to non-restrictive resources. The results indicated that there was a significant positive correlation among species,and the community was at a stable stage, showed strong ability to human interference. Source
Chen D.X.,Chongqing Academy of Chinese Materia Medica |
Chen D.X.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
Chen D.X.,Chongqing Key Laboratory of Chinese Medicine Resources |
Chen D.X.,Chinese Academy of Sciences |
And 12 more authors.
Genetics and Molecular Research
Lonicera macranthoides is an important traditional Chinese herb. The lack of information regarding the genetic structure and genetic relationships among its cultivars has hindered the conservation and utilization of this resource. This study used start codon targeted markers to assess the genetic diversity and other genetic characteristics of five single-variety L. macranthoides populations in China. Using 22 primers produced a total of 266 bands, of which 227 were polymorphic, indicating a high level of polymorphism. At the species level, genetic diversity was high: percentage of polymorphic loci (PPB) = 85.34%, effective number of alleles (NE) = 1.3479, Nei’s gene diversity (H) = 0.2075, and Shannon’s information index (Hsp, species level) = 0.3198. However, at the varietal population level, genetic diversity was lower, with averages of: PPB = 19.74%, NE = 1.0946, H = 0.0561, Hpop = 0.0850 (population level). Nei’s genetic differentiation coefficient was 0.7319, which is consistent with Shannon’s population genetic differentiation coefficient (0.7324). This indicates that most of the genetic variation in this species exists among the varietal populations. The differentiation among varieties may have been caused by artificial selection, mode of reproduction, and barriers to gene flow (0.1831). The genetic similarity coefficient ranged from 0.7222 to 0.9419. Phylogenetic analysis showed the five varieties to form two major clades. Results suggest that cultivar breeders should strengthen the exchange of germplasm and increase the mutual penetration of useful genes, which would broaden the hereditary basis of L. macranthoides. © FUNPEC-RP. Source
Chen D.-X.,Chinese Institute of Materia Medica |
Chen D.-X.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
Wang Y.,Chinese Institute of Materia Medica |
Wang Y.,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica |
And 6 more authors.
Chinese Traditional and Herbal Drugs
Objective: To investigate the genetic diversity and genetic structure among three types of Coptis chinensis by ISSR. Methods: Ninety individuals of the three types of C. chinensis were employed for inter-simple sequence repeat (ISSR) analysis. The parameters were calculated by POPGENE 1.31. Results: A total of 164 bands were amplified by 22 primers, among which 108 were polymorphic; A relatively high level of genetic diversity was revealed at species level: PPB = 65.85%, N e = 1.2298, H = 0.1440, and H sp = 0.2302; while at type level, PPB = 55.08%, N e = 1.2189, H = 0.1360, and H pop = 0.2151; The Nei's coefficient of genetic differentiation was 0.0561, which was consistent with the Shannon's coefficient of genetic differentiation (0.0652). Most of the genetic variation existed among types; The gene flow (N m = 8.4054) was high among cultivars, indicating that the degree of genetic differentiation was lower; Genetic similarity coefficient (I) changed from 0.9831 to 0.9897. By UPGMA clustering analysis and Nei's genetic distance, the cluster analysis based on ISSR and classification of morphology were almost the same. Conclusion: The relatively high genetic diversity of C. chinensis provides the basis for breeding new varieties. Source