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Yuzhong Chengguanzhen, China

Zhang Z.,Southwest University | Zhang Z.,Chongqing Center for Clinical Laboratory | Wang R.,Southwest University | Xie J.,Southwest University
Current Microbiology | Year: 2015

Mycobacterium smegmatis mc2155 MSMEG_3705 gene was annotated to encode a transporter protein that contains 12 alpha-helical transmembrane domains. We predicted MSMEG_3705 encoding a major facilitator superfamily (MFS) member. To confirm the prediction, the M. smegmatis mc2155 MSMEG_3705 gene was deleted. The MSMEG_3705 deletion mutant strain M. smegmatis mc2155 ∆MSMEG_3705 was more sensitive to capreomycin. Moreover, M. smegmatis mc2155 ∆MSMEG_3705 strain accumulated more ethidium bromide intracellular than wild-type M. smegmatis mc2155. Quite unexpectedly, M. smegmatis mc2155 ∆MSMEG_3705 grew faster than the wild-type M. smegmatis mc2155. The upregulation of the expression of MSMEG_3706, a gene encoding isocitrate lyase downstream MSMEG_3705, in the deletion mutant, might underlie such faster growth in the mutant. The study showed that MSMEG_3705 encodes a genuine MFS member and plays significant role in bacterial growth and antibiotics resistance. © 2015, Springer Science+Business Media New York.

Xu D.,Chongqing EYE and ENT Hospital | Yi H.,Chongqing EYE and ENT Hospital | Yu S.,Chongqing EYE and ENT Hospital | Li X.,Chongqing Center for Clinical Laboratory | And 2 more authors.
PLoS ONE | Year: 2016

Purpose To investigate the association of C5 SNPs with proliferative diabetic retinopathy (PDR) of type 2 diabetes (T2D). Methods A total of four C5 SNPs including rs2269067, rs7040033, rs1017119 and rs7027797 were genotyped in 400 PDR patients with T2D (cases) and 600 non- proliferative diabetic retinopathy PDR (NPDR) with T2D patients (controls) by using PCR-RFLP method. mRNA expression was examined by real-time PCR. Cytokine production was detected by ELISA. Results The frequency of GG genotype of C5 rs2269067 was significantly increased in cases compared with controls (Pc = 3.4×10-5, OR = 1.87). And C5mRNA expression was significantly increased in rs2269067 GG cases as compared with CG or CC cases (P = 0.003, P = 0.001, respectively). Moreover, the production of IL-6 was significantly increased in rs2269067 GG cases compared to CG cases or CC cases (P = 0.002, P = 0.001, respectively). Conclusions C5 rs2269067 GG genotype confers risk for PDR of T2D in Chinese han population and is associated with an elevated C5 mRNA expression and an increased IL-6 production. © 2016 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Wang R.,Southwest University | Zhang Z.,Southwest University | Zhang Z.,Chongqing Center for Clinical Laboratory | Xie L.,Southwest University | Xie J.,Southwest University
Critical Reviews in Eukaryotic Gene Expression | Year: 2015

Major facilitator superfamily (MFS) is an important secondary membrane transport protein superfamily conserved from prokaryotes to eukaryotes. The MFS proteins are widespread among bacteria and are responsible for the transfer of substrates. Pathogenic Mycobacterium MFS transporters, their distribution, function, phylogeny, and predicted crystal structures were studied to better understand the function of MFS and to discover specific inhibitors of MFS for better tuberculosis control. © 2015 Begell House, Inc.

Li X.,Chongqing Center for Clinical Laboratory | Li X.,Chongqing Medical University | Xia H.,Chongqing Medical University | Wang D.,Chongqing Medical University | And 2 more authors.
BioMed Research International | Year: 2015

The aim of our study was to increase the detection rate of fetal cardiac malformations for congenital heart disease (CHD). The ultrasonic and microanatomical methods were combined to study the CHD cases firstly, which could provide the microsurgical anatomical basis to the prenatal ultrasonic diagnosis which was used in suspected CHD and help the sonographer to improve the quality of fetal cardiac diagnosis. We established the ultrasonic standard section of the 175 complex CHD cases and collected the fetal echocardiography image files. The induced/aborted fetuses were fixed by 4% paraformaldehyde and dissected by the ultrasonic microsurgical anatomy. This research could obtain the fetal cardiac anatomic cross-sectional images which was consistent with the ultrasonic standard section and could clearly show the internal structure of the vascular malformation that optimized the ultrasound examination individually. This method could directly display the variation of the CHD fetal heart clearly and comprehensively help us to understand the complex fetal cardiac malformation from the internal structure of the vascular malformation which was consolidated by the anatomical basis of the fetal heart. This study could improve the integrity and accuracy of the prenatal cardiac ultrasound examination tremendously. © 2015 Xiaosong Li et al.

Long Q.,Southwest University | Long Q.,Chongqing Center for Clinical Laboratory | Huang C.,Southwest University | Huang C.,Chongqing Medical University | And 2 more authors.
Critical Reviews in Eukaryotic Gene Expression | Year: 2013

Acinetobacter baumannii is an important opportunist pathogen, due to severe antibiotic resistance and nosocomial infection. The epidemiology and antibiotic resistance of A.baumannii have been extensively reviewed, but the pathogenesis and virulence remain unclear. Proteomics analysis has been applied to study the mechanism of drug resistance, biofilm, micronutrient acquisition, and the extracellular compartment. This review summarizes applications of proteomics in A. baumannii, aiming to summarize novel insights into the mechanism of A. baumannii pathogenesis and drug resistance. © 2013 Begell House, Inc.

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