Yuzhong Chengguanzhen, China
Yuzhong Chengguanzhen, China

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Zhang Z.,Southwest University | Zhang Z.,Chongqing Center for Clinical Laboratory | Wang R.,Southwest University | Xie J.,Southwest University
Current Microbiology | Year: 2015

Mycobacterium smegmatis mc2155 MSMEG_3705 gene was annotated to encode a transporter protein that contains 12 alpha-helical transmembrane domains. We predicted MSMEG_3705 encoding a major facilitator superfamily (MFS) member. To confirm the prediction, the M. smegmatis mc2155 MSMEG_3705 gene was deleted. The MSMEG_3705 deletion mutant strain M. smegmatis mc2155 ∆MSMEG_3705 was more sensitive to capreomycin. Moreover, M. smegmatis mc2155 ∆MSMEG_3705 strain accumulated more ethidium bromide intracellular than wild-type M. smegmatis mc2155. Quite unexpectedly, M. smegmatis mc2155 ∆MSMEG_3705 grew faster than the wild-type M. smegmatis mc2155. The upregulation of the expression of MSMEG_3706, a gene encoding isocitrate lyase downstream MSMEG_3705, in the deletion mutant, might underlie such faster growth in the mutant. The study showed that MSMEG_3705 encodes a genuine MFS member and plays significant role in bacterial growth and antibiotics resistance. © 2015, Springer Science+Business Media New York.


Long Q.,Southwest University | Long Q.,Chongqing Center for Clinical Laboratory | Huang C.,Southwest University | Huang C.,Chongqing Medical University | And 2 more authors.
Critical Reviews in Eukaryotic Gene Expression | Year: 2013

Acinetobacter baumannii is an important opportunist pathogen, due to severe antibiotic resistance and nosocomial infection. The epidemiology and antibiotic resistance of A.baumannii have been extensively reviewed, but the pathogenesis and virulence remain unclear. Proteomics analysis has been applied to study the mechanism of drug resistance, biofilm, micronutrient acquisition, and the extracellular compartment. This review summarizes applications of proteomics in A. baumannii, aiming to summarize novel insights into the mechanism of A. baumannii pathogenesis and drug resistance. © 2013 Begell House, Inc.


Wang R.,Southwest University | Zhang Z.,Southwest University | Zhang Z.,Chongqing Center for Clinical Laboratory | Xie L.,Southwest University | Xie J.,Southwest University
Critical Reviews in Eukaryotic Gene Expression | Year: 2015

Major facilitator superfamily (MFS) is an important secondary membrane transport protein superfamily conserved from prokaryotes to eukaryotes. The MFS proteins are widespread among bacteria and are responsible for the transfer of substrates. Pathogenic Mycobacterium MFS transporters, their distribution, function, phylogeny, and predicted crystal structures were studied to better understand the function of MFS and to discover specific inhibitors of MFS for better tuberculosis control. © 2015 Begell House, Inc.


Du Y.,Chongqing Center for Clinical Laboratory | Du Y.,Luzhou Medical College | Li T.,Chongqing Center for Clinical Laboratory | Wan Y.,Chongqing Center for Clinical Laboratory | And 2 more authors.
Critical Reviews in Eukaryotic Gene Expression | Year: 2014

The quick spread of nosocomial bacterial infections and the increasing prevalence of drugresistant strains make the development of novel drugs for pathogens an urgent priority. Quorum sensing (QS) is a communication mechanism used by bacteria to recognize population density fluctuations and control gene expression, which play a critical role both in intraspecies and interspecies communications and regulates microbe-host interactions. Low-molecular-weight signal compounds, such as acyl-homoserine lactone and autoinducing peptide, are used by QS to control the expression of different pathogenic factors. Thus QS-and QS signal molecules in particular-is an attractive target for developing novel antimicrobial methods. Quorum-quenching enzymes, which hydrolyze or modify signal molecules in QS circuit systems to inhibit the expression of bacteria virulence factors, have been identified both in prokaryotes and eukaryotes. Understanding the mechanism of action of quorum-quenching enzymes also provides a promising means to control bacterial infection. This review first introduces the novel principle underling signal-based QS systems in several important pathogens and then focuses on the newly identified quorum-quenching enzymes, including lactonases, acylases, oxidoreductases, and paraoxonases; this summary introduces new concepts of antimicrobial infection. © 2014 Begell House, Inc.


Hu Q.,Chongqing Center for Clinical Laboratory | Hu Q.,Third Peoples Hospital of Chongqing | Tian H.,Chongqing Center for Clinical Laboratory | Tian H.,Third Peoples Hospital of Chongqing | And 7 more authors.
Transplantation Proceedings | Year: 2015

Background Interleukin-2 (IL-2) -330 T/G promoter polymorphism is involved in the acute rejection (AR) risk of kidney transplantation. However, results from published studies on the association between recipient IL-2-330 T/G polymorphism and AR risk are conflicting and inconclusive. Methods We searched Medline, Embase, Web of Science, and Cochrane Central Register from their inceptions through January 2015 for relevant studies. Data concerning publication information, population characteristics, and transplant information were extracted. Odds ratios (ORs) were calculated for the association between IL-2-330 T/G polymorphism and AR risk. Results This meta-analysis included 8 case-control studies with 1,405 cases of renal transplant recipients. The pooled estimate showed that IL-2-330 T/G polymorphism was not associated with AR risk: TT vs TG+GG: ORfixed, 0.93; 95% confidence interval [CI], 0.72-1.21; P =.60; GG vs TG+TT: ORfixed, 1.15; 95% CI, 0.76-1.72; P =.51; TG vs TT+GG: ORfixed, 1.01; 95% CI, 0.78-1.31; P =.91; T vs G: ORfixed, 0.93; 95% CI, 0.77-1.13; P =.48. None of subgroup analyses yielded significant results in the association between IL-2-330 T/G polymorphism and AR risk. Meta-regression confirmed that there was no significant correlation between the preselected trial characteristics and our study results. Conclusions This meta-analysis suggests that IL-2-330 T/G polymorphism may not be associated with AR risk in renal transplant recipients. © 2015 Elsevier Inc.


PubMed | Chongqing Center for Clinical Laboratory and Luzhou Medical College
Type: Journal Article | Journal: Critical reviews in eukaryotic gene expression | Year: 2014

The quick spread of nosocomial bacterial infections and the increasing prevalence of drugresistant strains make the development of novel drugs for pathogens an urgent priority. Quorum sensing (QS) is a communication mechanism used by bacteria to recognize population density fluctuations and control gene expression, which play a critical role both in intraspecies and interspecies communications and regulates microbe-host interactions. Low-molecular-weight signal compounds, such as acyl-homoserine lactone and autoinducing peptide, are used by QS to control the expression of different pathogenic factors. Thus QS--and QS signal molecules in particular--is an attractive target for developing novel antimicrobial methods. Quorum-quenching enzymes, which hydrolyze or modify signal molecules in QS circuit systems to inhibit the expression of bacteria virulence factors, have been identified both in prokaryotes and eukaryotes. Understanding the mechanism of action of quorum-quenching enzymes also provides a promising means to control bacterial infection. This review first introduces the novel principle underling signal-based QS systems in several important pathogens and then focuses on the newly identified quorum-quenching enzymes, including lactonases, acylases, oxidoreductases, and paraoxonases; this summary introduces new concepts of antimicrobial infection.

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