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Tanjiagang, China

p73 gene shares structural and functional similarities to p53 and plays an important role in modulating cell cycle arrest and apoptosis. A common non-coding polymorphism of p73 G4C14-to-A4T14 (rs2273953 and rs1801173) at exon 2 may affect gene expression, thus, it may lead to functional significance. The correlation of this polymorphism with clinicopathologic variables of patients with breast cancer has not been investigated. In this study, single-nucleotide polymorphisms (SNPs) of p73 G4C14-to-A4T14 were genotyped by Sequenom MassArray-iPLEX GOLD System in 170 patients with breast cancer. Data were analyzed via t test, chi-square test, and logistic regression analysis. There was no significant correlation between p73 G4C14-to-A4T14 polymorphisms and the patient characteristics, such as clinical TNM stage, menopausal status, axillary lymph node metastasis, pathological type, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2). No significant relationship was observed between the p73 G4C14-to-A4T14 polymorphism and p73 protein expression in cancer tissues. The frequency of GC/GC genotype in patients with triple-negative breast cancer (TNBC) was 78.9 %, that of patients with others was 57.6 %, and the difference had statistical significance (χ 2 = 5.74, P = 0.02). p73 G4C14-to-A4T14 polymorphisms were negatively correlated with chemosensitivity for anthracycline-based chemotherapy in breast cancer (P > 0.05). p73 G4C14-to-A4T14 polymorphisms are positively correlated with TNBC, and p73 gene may play a critical role in a novel therapeutic strategy to TNBC. Additional larger studies are required to test these hypotheses. © 2013 Springer Science+Business Media New York. Source

Zhou X.,Chongqing Medical University | Zhou X.,Chongqing Cancer Institute | Wu C.,Chongqing Medical University
Medical Oncology | Year: 2012

The aim of this study was to evaluate the association of p73 G4C14-A4T14 polymorphisms with susceptibility to breast cancer in Chongqing women of Han Nationality in China. In a case-control study, single-nucleotide polymorphisms of p73 G4C14-A4T14 at exon 2 were genotyped by Sequenom MassArray ® iPLEX GOLD System in 170 patients with breast cancer and 178 healthy controls. Data were analyzed via t test, Chi-square test, and logistic regression analysis. The distribution of p73 genotypes and allelotypes had no significant difference between patients with breast cancer and healthy controls (χ2 = 2.750, P = 0.253; χ2 = 2.195, P = 0.138). More risk of developing triple negative breast cancer (TNBC) was found in the individuals who carried with GC/GC genotype than individuals carried with GC/AT and AT/AT genotypes (OR = 2.99; 95 % CI, 1.30-6.89; P = 0.010). p73 G4C14-A4T14 polymorphisms are closely associated with the increased risk for TNBC in Chongqing women of Han Nationality in China; GC/GC genotype is susceptible genotype for TNBC in Chongqing women of Han Nationality in China. The patients with breast cancer who carried with GC/GC genotype may have bad prognosis. Additional larger studies are required to confirm these findings. © 2012 Springer Science+Business Media, LLC. Source

Jiang Q.M.,Chongqing Cancer Institute
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2012

To investigate the clinicopathologic features, immunophenotyping, differential diagnoses and prognosis of histiocytic sarcoma (HS). The clinical and pathologic findings of 4 cases of HS were reviewed. The samples were used for paraffin section, HE stain, immunohistochemistry stain by EnVision method, electron microscope observation. Follow-up information was available in all patients. The age of patients, 2 males and 2 females, ranged from 22 to 65 years old (median, 43.25 years). The sites of involvement included lymph node (2 cases), skin or soft tissue (1 case) and colon (1 case). The tumor cells were widespread infiltration, diffused distribution, no adhesion to each other. Tumor cells were middling and large, round, orbicular-ovate, polygon, epithelium appearance, plentiful cytoplasm and acidophilia, cystose. Nucelus was round, orbicular-ovate, dissymmetry. Nuclear chromatin was vacuole appearance, basophilia nucleolus, caryocinesia and pathological mitotic figure. Three of the cases showed conjugate nuclei, increased pleomorphism with multinucleated tumor giant cell formation. Focal cytoplasmic with foamy appearance was identified in 2 cases. One case demonstrated foci of spindly sarcomatoid appearance. Hemophagocytosis was identified in 2 cases. The tumor cells of 4 cases were often accompanied by various numbers of inflammatory cells. Immunohistochemical study showed that all cases were diffusely positive for α-1-ACT, CD68, CDl63 and lysozyme. Three of 4 cases also expressed CD45, CD45RO. The electron microscope results of 4 cases showed that the tumor cells were plentiful cytoplasm and a few cytolysosome in the cytoplasm, and no birbeck cytorrhyctes, cell-cell junction and digitation. Amongst the 4 patients with follow-up information available, three died of the disease 6-13 months after diagnosis. One patient, whose lesion was localized at the skin and soft tissue, survived at the present time. HS was a scarce malignant tumor with mature histiocyte morphology and immunophenotype character. The diagnosis should be based on tissue morphology, immunohistochemistry and electron microscope observation to exclude other disorders. Source

Objective: To investigate the effect of mycophenolate mofetil (MMF) on transforming growth factor-β1 (TGF-β1) gene expression in bleomycin (BLM)-induced mouse model of pulmonary fibrosis. Methods: C57BL/6 mice were randomly divided into six groups, six for each, i.e. normal control, MMF control, BLM model as well as low (20 mg/kg), moderate (60 mg/kg) and high (100 mg/kg) dose MMF treatment groups. The mice in BLM model and three MMF treatment groups were intratracheally instilled with BLM (6 mg/kg), while those in normal control and MMF control groups with sterile physiological saline at an equal volume. One day after instillation, the mice in MMF control and MMF treatment groups were treated with MMF by lavage at the calculated dosages based the bodyweight, once a day for 14 d, while those in normal control and BLM model groups with double distilled water at an equal volume. Lung specimens of mice were collected on day 16 after lavage, observed for pulmonary fibrosis by HE and Masson staining and subjected to Aschcroft scoring. The TGF-β1 mRNA transcription level in lung tissue was determined by RT-PCR, while the protein expression level by Western blot. Results: BLM-induced pulmonary fibrosis in mouse model was improved significantly, of which the Ashcroft score was significantly higher than that in normal control group (P < 0.01), indicating that the mouse model of pulmonary fibrosis was established successfully. The lung tissue lesion of mice in high dose MMF treatment group was relieved, while the inflammatory cell infiltration and collagen deposition decreased, and the Ashcroft score decreased significantly (P = 0.000). Both the mRNA transcription and protein expression levels of TGF-β1 was significantly lower in high dose MMF treatment group than in BLM model group (P < 0.05), while showed no significant difference in low and moderate dose MMF treatment and BLM model groups, or in MMF control and normal control groups (P > 0.05). Conclusion: High dose (100 mg/kg) MMF significantly inhibited the mRNA transcription and protein expression of TGF-β1 in lung tissue of mice with BLM-induce pulmonary fibrosis, which might be an ideal drug for pulmonary fibrosis. Source

Zeng L.,Chongqing Medical University | Wang W.,Chongqing Medical University | Wang W.,Chongqing Cancer Institute | Rong X.-F.,Chongqing Medical University | And 4 more authors.
International Immunopharmacology | Year: 2014

Cartilage degradation is the most predominant pathological change during osteoarthritis (OA). Furthermore, accumulating evidence suggests that an excess of matrix metalloproteinase-13 (MMP-13) plays a critical role in the breakdown of cartilage. Here, the effects of Icariin on the expression of MMP-13 in IL-1β-induced SW 1353 chondrosarcoma cells were investigated. In addition, the in vivo effects of Icariin on an experimental rat model of OA induced by anterior cruciate ligament transection (ACLT) was examined. SW1353 chondrosarcoma cells were pretreated with or without Icariin and MAPK and Wnt/β-catenin signaling pathway inhibitors, then were stimulated with IL-1β. In rats, experimental OA was induced by ACLT. These rats then received intra-articular injections of vehicle, signaling pathway inhibitors, and/or Icariin. Expression of MMP-13, phosphorylated p38, phosphorylated JNK, and β-catenin were verified by western blotting. In addition, levels of MMP-13 mRNA were detected using quantitative real-time PCR. In histological analyses, treatment with Icariin reduced the number of cartilage lesions present. In addition, treatment with Icariin was associated with lower levels of phosphorylated p38, phosphorylated JNK, and β-catenin in both IL-1β-induced SW1353 chondrosarcoma cells and in the rat OA model. Furthermore, the suppressive effect of Icariin on MMP-13 was greater than that exhibited by other signaling pathway inhibitors. Overall, these data suggest that Icariin has therapeutic potential for the treatment of OA. © 2013 Elsevier B.V. Source

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