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Wu M.,Chengdu University of Traditional Chinese Medicine | Wu M.,China Military Institute of Chinese Medicine | Qu F.,Clinical Examination Center | Zhao Y.,Chengdu University of Traditional Chinese Medicine | And 12 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2016

As a healthcare-associated pathogen with the resistance to anti-bacterial agent, P. aeruginosa has caused prevalent public burden and should not be ignored. Facing the realistic situation, developing novel anti-P. aeruginosa agents is urgent. Leaves of Dracontomelon dao were extensively used in southern China to treat various infectious diseases 1000 years ago. And in the study, the heat flow power-time (HFP-time) curves generated by P. aeruginosa which were disturbed by the five fractions (PE, CHCl3, EtOAc, n-BuOH and Vestiges fraction) from them were investigated through microcalorimetry, and then, some thermal kinetic parameters were obtained from the curves to characterize the metabolism of P. aeruginosa. The parameters were analyzed by principal component analysis (PCA), and the anti-P. aeruginosa activities of the five fractions were systematically compared and evaluated. The results showed that five fractions all expressed respective anti-P. aeruginosa effects in a dose-dependent manner and especially the performance of EtOAc fraction with half-inhibitory concentration (IC50) of 18.06 μg mL-1. Simultaneously, the prospective performance of EtOAc fraction was confirmed by turbidimetry. Based on the promising anti-P. aeruginosa activities of EtOAc fraction, it could be developed as a novel anti-bacterial agent used in practice for treating certain infectious diseases. © 2015 Akadémiai Kiadó, Budapest, Hungary. Source


Wu M.,Chengdu University of Traditional Chinese Medicine | Wu M.,China Military Institute of Chinese Medicine | Qu F.,Clinical Examination Center | Zhao Y.,Chengdu University of Traditional Chinese Medicine | And 13 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2015

As a healthcare-associated pathogen with the resistance to anti-bacterial agent, P. aeruginosa has caused prevalent public burden and should not be ignored. Facing the realistic situation, developing novel anti-P. aeruginosa agents is urgent. Leaves of Dracontomelon dao were extensively used in southern China to treat various infectious diseases 1000 years ago. And in the study, the heat flow power–time (HFP–time) curves generated by P. aeruginosa which were disturbed by the five fractions (PE, CHCl3, EtOAc, n-BuOH and Vestiges fraction) from them were investigated through microcalorimetry, and then, some thermal kinetic parameters were obtained from the curves to characterize the metabolism of P. aeruginosa. The parameters were analyzed by principal component analysis (PCA), and the anti-P. aeruginosa activities of the five fractions were systematically compared and evaluated. The results showed that five fractions all expressed respective anti-P. aeruginosa effects in a dose-dependent manner and especially the performance of EtOAc fraction with half-inhibitory concentration (IC50) of 18.06 μg mL−1. Simultaneously, the prospective performance of EtOAc fraction was confirmed by turbidimetry. Based on the promising anti-P. aeruginosa activities of EtOAc fraction, it could be developed as a novel anti-bacterial agent used in practice for treating certain infectious diseases. © 2015 Akadémiai Kiadó, Budapest, Hungary Source


Wei S.-Z.,Chengdu University of Traditional Chinese Medicine | Luo S.-Q.,302 Hospital of Peoples Liberation Army | Wang J.,Chengdu University of Traditional Chinese Medicine | Wang J.-B.,China Military Institute of Chinese Medicine | And 15 more authors.
Chinese Journal of Integrative Medicine | Year: 2015

Objective: To investigate the possible mechanism of San-Cao Granule (SCG, 三草颗粒) mediating antiliver fifibrosis. Methods: A total of 60 male Sprague-Dawley rats were randomly divided into the normal control group, porcine serum-treated group, ursodesoxycholic acid (UDCA, 60 mg/kg), SCG (3.6 g/kg) group, SCG (1.8 g/kg) group and SCG (0.9 g/kg) group, with 10 rats in each group. Liver fifibrosis was induced with porcine serum by intraperitoneal injection for 8 weeks, except for the normal control group. Then, the rats in the three SCG-treated groups and UDCA group were administered SCG and UDCA respectively for 4 weeks. The serum levels of alanine transaminase (ALT), aspartate transaminase (AST), albumin (ALB), total bilirubin (TBIL), hyaluronic acid (HA), laminin (LN), and type IVcollagen (IVC) were examined using commercial kits and hepatic histopathology was examined with hematoxylin and eosin and Masson staining. Moreover, the protein expression levels of high mobility group box-1 protein (HMGB1), transforming growth factor β1 (TGF-β1), phosphorylated mothers against decapentaplegic homolog 3 (p-Smad3), Smad7, toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-kappa B (NF-κB) and α-smooth muscle actin (α-SMA) were determined by western blot, immunohistochemistry and real time quantitativereverse transcription polymerase. Results: Both SCG (3.6 and 1.8 g/kg) and UDCA signifificantly ameliorated the liver fifibrosis induced by porcine serum as indicated by retarding the serum levels increasing of ALT, AST, TBIL, HA, LN and IVC and preventing the serum level reducing of ALB compared with the model group (all P<0.01). Meanwhile, the collagen deposition was attenuated by SCG and UDCA treatment. Furthermore, SCG markedly reduced the expressions of HMGB1, TGF-β1, p-Smad3, TLR4, MyD88, NF-κB and α-SMA, and enhanced the expression of the Smad7 compared with the model group (all P<0.01). Conclusion: SCG ameliorates hepatic fifibrosis possibly through inhibiting HMGB1, TLR4/NF-κB and TGF-β1/Smad signaling pathway. © 2015 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg Source


Ke X.-M.,Chengdu University of Traditional Chinese Medicine | Ke X.-M.,Chongqing Academy of Chinese Traditional Materia Medica | Yang R.-P.,Chengdu University of Traditional Chinese Medicine | Yang R.-P.,Chongqing Academy of Chinese Traditional Materia Medica | And 7 more authors.
Chinese Traditional and Herbal Drugs | Year: 2014

Objective: To explore the feasibility of self-emulsifying drug delivery system (SEDDS) for multicomponents simuitaneous solubilization. Methods: The curcumin (Cur) components were used as model drug, D-optimal mixture design was used to optimize SEDDS prescription, and the contents of bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC), and Cur, SEDDS particle size, and emulsifying time were made as indicators to select and evaluate SEDDS, so as to explore the feasibility of SEDDS for the multicomponents simuitaneous solubilization. Results: The optimal SEDDS prescription was Obleique CC497-Tween 20-Transcutol P (0.21:0.50:0.29), SEDDS particle size was (248.8 ± 3.4) nm, and emulsifying time was (70 ± 1) s. At 37°C, the maximum loading capacities of SEDDS for BDMC, DMC, and Cur were 2.998, 12.220, and 52.561 mg/g, respectively, and the solubilities in water were 0.107, 0.661, and 1.648 mg/mL. Conclusion: SEDDS can realize the solubilization of multicomponent simultaneously. Source


Jia C.-Y.,Chengdu University of Traditional Chinese Medicine | Jia C.-Y.,Chongqing Academy of Chinese Traditional Materia Medica | Li W.,Chengdu University of Traditional Chinese Medicine | Zhang C.-H.,Chongqing Academy of Chinese Traditional Materia Medica | And 6 more authors.
Chinese Traditional and Herbal Drugs | Year: 2016

Objective: To provide a reference for the industrial production of the extraction process of Compound Baihuang Xiere Zhili Tablets (CBXZT) by exploring the optional extraction process parameters based on determining the index weight. Methods: With the extraction capacity of anemoside B4, berberine hydrochloride, aesculin, aesculetin, scopoletin, and ratio of extraction as comprehensive evaluation indexes, the weighting coefficient in six synthetic evaluation indexes was determined by Analytic Hierarchy Process (AHP), Criteria importance through intercriteria correlation (CRITIC) method, and mixed weighted AHP-CRITIC. And ethanol-extracting process was optimized by orthogonal test. Results: The excellent linearity with correlation coefficients were 121.1-1211.0, 82.2-822.0, 22.3-223.0, 15.42-154.2, and 1.6-16.0 μg/mL, r of 0.9997-0.9999 was obtained, and the average recoveries of the five compounds were 99.23%-100.63% with all RSD values less than 3.0%. Mixed weighted AHP-CRITIC was more scientific, reasonable, and stable than AHP and CRITIC. The optimal process parameters were confirmed to be decocting for thrice with 2 h in each by eight times the amount of 60% ethanol, according to the weight coefficient determined by comprehensive evaluation. Conclusion: These optimized technologies are stable, feasible, and suitable for standardized production of CBXZT. The weight coefficient determined by mixed weighted AHP-CRITIC was objective, real and could reflect the compound compatibility information. These optimized technologies are stable, feasible and suitable for standardized production of CBXZT. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved. Source

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