Gu Y.R.,Sichuan Agricultural University |
Li M.Z.,Sichuan Agricultural University |
Zhang K.,Sichuan Agricultural University |
Chen L.,Chongqing Academy of Animal Science |
And 3 more authors.
Journal of Animal Breeding and Genetics | Year: 2011
To normalize a set of quantitative real-time PCR (q-PCR) data, it is essential to determine an optimal number/set of housekeeping genes, as the abundance of housekeeping genes can vary across tissues or cells during different developmental stages, or even under certain environmental conditions. In this study, of the 20 commonly used endogenous control genes, 13, 18 and 17 genes exhibited credible stability in 56 different tissues, 10 types of adipose tissue and five types of muscle tissue, respectively. Our analysis clearly showed that three optimal housekeeping genes are adequate for an accurate normalization, which correlated well with the theoretical optimal number (r≥0.94). In terms of economical and experimental feasibility, we recommend the use of the three most stable housekeeping genes for calculating the normalization factor. Based on our results, the three most stable housekeeping genes in all analysed samples (TOP2B, HSPCB and YWHAZ) are recommended for accurate normalization of q-PCR data. We also suggest that two different sets of housekeeping genes are appropriate for 10 types of adipose tissue (the HSPCB, ALDOA and GAPDH genes) and five types of muscle tissue (the TOP2B, HSPCB and YWHAZ genes), respectively. Our report will serve as a valuable reference for other studies aimed at measuring tissue-specific mRNA abundance in porcine samples. © 2011 Blackwell Verlag GmbH.
Liu L.,China Agricultural University |
Liu L.,Chongqing Academy of Animal science |
Pang C.,China Agricultural University |
Wu S.,China Agricultural University |
Dong R.,China Agricultural University
Process Safety and Environmental Protection | Year: 2015
An air-recirculated stripping involved two processes and did not require any pretreatment. First, stripping CO2 decreased the buffer capacity of the anaerobic digestate, thereby reducing the amount of lime used to achieve a high pH. Second, lime was added to increase pH and remove ammonia from the anaerobic digestate of pig manure. pH increased from 8.03 to 8.86 by stripping CO2 in the first process (gas-to-liquid ratio = 180) and further reached 12.38 in the second process (gas-to-liquid ratio = 300). During process optimization, the maximum ammonia removal efficiency reached 96.78% with a lime dose of 22.13 g. The value was close to 98.25%, which was the optimal result predicted by response surface methodology using the software Design-Expert 8.05b. All these results indicated that air-recirculated stripping coupled with absorption was a promising technology for the removal and recovery of nitrogen in the anaerobic digestate of pig manure. © 2014 The Institution of Chemical Engineers.
PubMed | Tsinghua University, Chinese PLA General Hospital and Chongqing Academy of Animal Science
Type: | Journal: Acta oto-laryngologica | Year: 2017
In this investigation, a large mammal, Rongchang pigs were used to successfully establish a research platform for cochlear implant study on the routine use of it in clinic.The aim of this study was to establish a standard method of cochlear implant in a large mammal-pig.Rongchang pigs were selected, then divided into two groups: normal-hearing group (Mitf+/+) and mutation group with hearing loss (Mitf -/-). Cochlear implants were used and ABR and EABR were recorded. The implanted electrodes were observed by X-ray and HE stains.The success with cochlear implant and the best electrode position could be defined in all animals, the coiling of the cochlea reached 1.5-1.75 turns. Immediately after the operation of cochlear implants, the ABR threshold of the operated ear (right) could not be derived for each frequency at 120dB SPL. Moreover, 7 days after surgery, the low-frequency ABR threshold of the operated ear (right) could be derived partly at 100dB SPL, but the high-frequency ABR threshold could not be derived at 120dB SPL. Immediately or 1 week after cochlear implants, the EABR threshold was 90CL in the Mitf+/+group. This was obviously lower than the 190CL in the Mitf -/- group.
Ge L.,Chongqing Academy of Animal science |
Ge L.,University of Manitoba |
Ge L.,Manitoba Institute of Child Health |
Ge L.,Key Laboratory of Pig Industry science |
And 9 more authors.
International Journal of Nanomedicine | Year: 2014
Nanosilver particles (NSPs), are among the most attractive nanomaterials, and have been widely used in a range of biomedical applications, including diagnosis, treatment, drug delivery, medical device coating, and for personal health care. With the increasing application of NSPs in medical contexts, it is becoming necessary for a better understanding of the mechanisms of NSPs' biological interactions and their potential toxicity. In this review, we first introduce the synthesis routes of NSPs, including physical, chemical, and biological or green synthesis. Then the unique physiochemical properties of NSPs, such as antibacterial, antifungal, antiviral, and anti-inflammatory activity, are discussed in detail. Further, some recent applications of NSPs in prevention, diagnosis, and treatment in medical fields are described. Finally, potential toxicology considerations of NSPs, both in vitro and in vivo, are also addressed. © 2014 Ge et al.
Liu J.,Chinese Academy of Agricultural Sciences |
Liu J.,Inner Mongolia Academy of Agricultural and Animal Husbandry science |
Zhang L.,Chinese Academy of Agricultural Sciences |
Xu L.,Chinese Academy of Agricultural Sciences |
And 8 more authors.
BMC Genomics | Year: 2013
Background: In recent years, genome-wide association studies have successfully uncovered single-nucleotide polymorphisms (SNPs) associated with complex traits such as diseases and quantitative phenotypes. These variations account for a small proportion of heritability. With the development of high throughput techniques, abundant submicroscopic structural variations have been found in organisms, of which the main variations are copy number variations (CNVs). Therefore, CNVs are increasingly recognized as an important and abundant source of genetic variation and phenotypic diversity.Results: Analyses of CNVs in the genomes of three sheep breeds were performed using the Ovine SNP50 BeadChip array. A total of 238 CNV regions (CNVRs) were identified, including 219 losses, 13 gains, and six with both events (losses and gains), which cover 60.35 Mb of the sheep genomic sequence and correspond to 2.27% of the autosomal genome sequence. The length of the CNVRs on autosomes range from 13.66 kb to 1.30 Mb with a mean size of 253.57 kb, and 75 CNVRs events had a frequency > 3%. Among these CNVRs, 47 CNVRs identified by the PennCNV overlapped with the CNVpartition. Functional analysis indicated that most genes in the CNVRs were significantly enriched for involvement in the environmental response. Furthermore, 10 CNVRs were selected for validation and 6 CNVRs were further experimentally confirmed by qPCR. In addition, there were 57 CNVRs overlapped in our new dataset and other published ruminant CNV studies.Conclusions: In this study, we firstly constructed a sheep CNV map based on the Ovine SNP50 array. Our results demonstrated the differences of two detection tools and integration of multiple algorithms can enhance the detection of sheep genomic structure variations. Furthermore, our findings would be of help for understanding the sheep genome and provide preliminary foundation for carrying out the CNVs association studies with economically important phenotypes of sheep in the future. © 2013 Liu et al.; licensee BioMed Central Ltd.
Yong W.J.,Chongqing Academy of Animal science |
Jing L.,Chongqing Academy of Animal science |
Jiugang Z.,Chongqing Academy of Animal science |
Lei C.,Chongqing Academy of Animal science |
Yonggang L.,Yunnan Agricultural University
Cytokine | Year: 2012
The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Wujin and Large White pigs. One novel gene differentially expressed was identified through quantitative real time PCR and the cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 507 amino acids that shares high homology with the protection of telomeres 1 isoform 4 (POT1) of human (86%)-so that this gene can be defined as swine POT1 gene. This gene is structured in 12 exons and 11 introns as revealed by computer-assisted analysis. The tissue expression analysis indicated that the swine POT1 gene is differentially expressed in tissues including muscle, heart, liver, fat, kidney, lung, pancreas and spleen. Our experiment is the first to establish the primary foundation for further research on the swine POT1 gene. © 2012 Elsevier Ltd.
Yang L.,Chongqing Academy of Animal science
Chinese Journal of Biologicals | Year: 2013
Phage, a virus using different bacteria as hosts, has strictly bacterial host specificity, and parasitizes only in the susceptible hosted bacteria. Without toxicity to animal or plant cells, phage has simple structure and a small quantity of gene, which becomes a fine operating system for molecular biology and genetic engineering. Based on phage display technology, phage particles are safe, reliable and have good immune effect. Satisfactory results in study on prophylactic and therapeutic vaccines indicate that, as a delivery vehicle, phage provides a new approach for developing novel vaccine. This paper reviews the advantage and prospect of phage particles in research on vaccine.
Liu L.,Chongqing Medical University |
Li D.,Chongqing Medical University |
Wang Y.,Chongqing Medical University |
Xu H.,Chongqing Medical University |
And 2 more authors.
International Urogynecology Journal and Pelvic Floor Dysfunction | Year: 2011
Introduction and hypothesis: Xenogeneic (porcine) extracellular matrix (ECM) scaffolds have been suggested as ideal biomaterials for regeneration medicine; however, ECM prepared from different tissue sources has shown distinctive biological properties. Therefore, a comprehensive understanding of biological characteristics of different tissue-derived ECM is essential in the design of scaffolds for pelvic reconstruction. Methods: We compared the biological properties of ECM derived from different tissue sources of Bama miniature pigs as a pelvic biological patch in terms of histological structure, water absorption ability, biodegradation ability, mechanical properties, antimicrobial activity, and biocompatibility in vitro. Results: Different ECM scaffolds have distinct structural differences, and all have good biocompatibility, and UBM exhibited better water uptake ability (above 500%), anti-biodegradation ability, mechanical properties, antimicrobial activity, and stem cell attachment properties than other tissue-derived ECM. Conclusions: Porcine UBM might serve as an ideal pelvic biological patch. © 2010 The International Urogynecological Association.
Yang J.L.,Chongqing Academy of Animal Science
Virology journal | Year: 2012
Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.
Liu L.,Chongqing Medical University |
Deng L.,Chongqing Medical University |
Wang Y.,Chongqing Medical University |
Ge L.,Chongqing Medical University |
And 3 more authors.
International Urogynecology Journal and Pelvic Floor Dysfunction | Year: 2012
Introduction and hypothesis: The present study set out to modify polypropylene vaginal surgical material using porcine urinary bladder matrix (UBM) in order to improve biocompatibility. The aim was to develop a compound scaffold that induced less vaginal erosion and to evaluate host immunoreactivity to this material in vivo. Methods: Forty-eight Sprague-Dawley rats were randomly divided into four equal groups. One group underwent a sham operation, and the other groups underwent vaginal implantation with different materials: UBM (U); UBM+ polypropylene (UP); or polypropylene (P). The host tissue response was determined by macro-observation, and by histological and immunohistochemical methods at 7, 14, 21, or 28 days after surgery. Results: The inflammation reaction was strongest throughout the entire observation time in Group P, but was weaker and had a tendency to decrease with time in Groups U and UP. The presence of the UBM material in the compound scaffold allowed the polypropylene to fuse with newly proliferating surrounding tissue and resulted in less rejection of the material by the host, as indicated by the reduced appearance of CD4-, and CD8-positive cells. Conclusions: Porcine UBM allowed mechanical isolation of polypropylene, and also reduced the immune reaction to polypropylene. This study suggests that the UBM+polypropylene compound scaffold may be a promising material for clinical use in pelvic reconstruction surgery. © The International Urogynecological Association 2012.