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Wu S.,CAS Dalian Institute of Chemical Physics | Wu S.,Chongqing Academy of Agricultural science | Meng Y.,CAS Dalian Institute of Chemical Physics | Cao X.,CAS Dalian Institute of Chemical Physics | Xue S.,CAS Dalian Institute of Chemical Physics
Algal Research | Year: 2016

Oxidative species and phytohormones are important factors in plants that can regulate stress responses. In this study, the content variations of four oxidative species, O2 -, H2O2, ClO-, and NO, and the phytohormones, salicylic acid (SA), jasmonic acid (JA), and folic acid (FA), were investigated in the microalgae Isochrysis zhangjiangensis under nitrogen deficiency. The O2 - and H2O2 contents were found to be positively correlated with the photosynthetic activity of the microalgae, while the opposite relationship was observed for NO and ClO-. In higher plants, there is a balance model for NO and ROS interactions, which revealed that the cooperation of NO and H2O2 regulates the pathogen-induced hypersensitive response-associated cell death. Because the NO and H2O2 levels in both nitrogen-replete and nitrogen-depleted I. zhangjiangensis were not simultaneously high, we proposed that as a unicellular lower plant, the microalgae I. zhangjiangensis follows this model and regulates the different types of intracellular oxidative species, especially when they were cultured from nitrogen sufficient condition to corresponding nitrogen deficient condition, to avoid cell death. Together with the corresponding changes in the SA, JA and FA contents in the nitrogen-stressed microalgae, we proposed that the balance model of oxidative species interactions, as well as the correlations among nitrogen assimilation, photosynthesis and the biosyntheses of SA, JA, and FA, may originally exist in algae, and plants have followed this model and maintained these correlations all along to protect themselves from stressful conditions during their evolutions from alga to higher plants. © 2016.

Zhang Y.,Chongqing University | Hu Z.,Chongqing University | Chu G.,Chongqing University | Huang C.,Chongqing University | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Eggplant (Solanum melongena L.) is an edible fruit vegetable cultivated and consumed worldwide. The purple eggplant is more eye-catching and popular for the health-promoting anthocyanins contained in the fruit skin. Two kinds of anthocyanin were separated and identified from purple cultivar (Zi Chang) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. To investigate the molecular mechanisms of anthocyanin accumulation in eggplant, the transcripts of anthocyanin biosynthetic and regulatory genes were analyzed in the fruit skin and the flesh of the purple cultivar and the white cultivar (Bai Xue). Compared with the other tissues, SmMYB1 and all anthocyanin biosynthetic genes except PAL were dramatically upregulated in the fruit skin of the purple cultivar. Overexpression of SmMYB1 activated abundant anthocyanin accumulation in the regenerating shoots of eggplant. These results prove that transcriptional activation of SmMYB1 accounts for constitutive upregulation of most anthocyanin biosynthetic genes and the onset of anthocyanin biosynthesis in the purple cultivar. © 2014 American Chemical Society.

Hu H.,Beijing University of Chemical Technology | Song H.-Q.,Beijing University of Chemical Technology | Yu B.-R.,Beijing University of Chemical Technology | Cai Q.,Beijing University of Chemical Technology | And 2 more authors.
Polymer Chemistry | Year: 2015

The development of safe and effective supramolecular polycations has attracted much attention. In this work, a series of novel cyclodextrin (CD)-based supramolecular assemblies were readily prepared via the host-guest interaction by assembling different adamantane-functionalized α-CD derivatives with multiple β-CD-cored polycations. The supramolecular assemblies were investigated in terms of their pDNA-binding capabilities, cytotoxicities, cellular internalization and gene transfection efficiencies in HEK293, HepG2 and C6 cell lines. The supramolecular polycations displayed low cytotoxicities, similar to those of the CD-cored carriers. After self-assemblies, the gene transfection efficiencies and antitumor abilities of the supramolecular carriers were significantly enhanced. The present study demonstrates that such supramolecular preparation of CD-based polycations could provide a flexible strategy for the design and development of new assemblies with high efficiency and low cytotoxicity. This journal is © 2015 The Royal Society of Chemistry.

Sun G.-M.,Jinan University | Yang P.-H.,Jinan University | Sun J.-H.,Chongqing Academy of Agricultural science | Cai J.-Y.,Jinan University
Faguang Xuebao/Chinese Journal of Luminescence | Year: 2011

Due to the unique and tunable optical properties, gold nanorods have potential and important applications in the biomedical field. In this paper, gold nanorods with the aspect ratio 8 were prepared, an outstanding fluorescence feature of the gold nanorods excited at 480 nm was observed in terms of a strong fluorescence emission at 707 nm and a relatively weak fluorescence at 560 nm. Based on their fluorescence properties, HepG2 cells were labeled by this type of gold nanorods, and were studied by using laser scanning confocal microscope. Green and red fluorescence images of gold nanorods were collected under the excitation of 488 nm. Furthermore, high-resolution atomic force microscopy was used to obtain the morphology and mechanical properties changes of the HepGz cells.

Song J.,Chinese Academy of Agricultural Sciences | Gu J.,Chinese Academy of Agricultural Sciences | Zhai Y.,Chongqing Academy of Agricultural science | Wu W.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Bioresource Technology | Year: 2013

The fungal strain LZM1 was isolated from activated sludge and found to be capable of utilizing nicosulfuron as the sole nitrogen source for growth. Based on morphological and internal transcribed spacer evaluations, LZM1 was identified as a Talaromyces flavus strain. Under optimum conditions (pH 6.1, 29°C), T. flavus LZM1 degraded 100% of the initially added nicosulfuron (100mgL-1) within 5d. T. flavus LZM1 was also found to be highly efficient in degrading tribenuron methyl, chlorsulfuron, bensulfuron methyl, ethametsulfuron methyl, cinosulfuron, and rimsulfuron. Metabolites from nicosulfuron degradation were identified by liquid chromatography mass spectrometry, and a possible degradation pathway was deduced. These results show that T. flavus LZM1 may possess potential to be used in bioremediation of nicosulfuron-contaminated environments. © 2013 Elsevier Ltd.

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