Chongqing Academy of Agricultural science

Chongqing, China

Chongqing Academy of Agricultural science

Chongqing, China
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PubMed | Chongqing Academy of Agricultural Science, Southwest University and Sichuan Agricultural University
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2016

There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of

PubMed | University of Sichuan, CAS Chengdu Institute of Biology, Chongqing Academy of Agricultural science and Biogas Institute of Ministry of Agriculture
Type: Journal Article | Journal: Journal of applied microbiology | Year: 2016

To reveal the microbial communities from Qinghai-Tibetan Plateau wetland soils that have the potential to be used in the utilization of cellulosic and chitinous biomass at low temperatures (25C).Soil samples collected from six wetlands on Qinghai-Tibetan Plateau were supplemented with or without cellulose and chitin flakes, and anaerobically incubated at 25 and 15C; high-throughput 16S rRNA gene sequencing was used to access the composition and localization (in the slurry and on the surface) of enriched microbial communities; a hypothetical model was constructed to demonstrate the functional roles of involved microbes mainly at genus level. Overall, microbial communities from Qinghai-Tibetan Plateau wetlands showed significant potential to convert both cellulose and chitin to methane at low temperatures; Clostridium III, Clostridium XIVa, Paludibacter, Parcubacteria, Saccharofermentans, Pelotomaculum, Methanosaeta, Methanobrevibacter, Methanoregula, Methanospirillum and Methanosarcina participated in methanogenic degradation of both cellulose and chitin through the roles of hydrolytic, saccharolytic and secondary fermenters and methanogens respectively. Acetotrophic methanogens were mainly enriched in the slurries, while hydrogenotrophic methanogens could be both in the slurries and on the surface.The composition and localization of microbial communities that could effectively convert cellulose and chitin to methane at low temperatures have been revealed by high-throughput 16S rRNA gene sequencing methods, and reviewing the literatures on the microbial pure culture helped to elucidate functional roles of significantly enriched microbes.This study will contribute to the understanding of carbon and nitrogen cycling of cellulose and chitin in cold-area wetlands and provide fundamental information to obtain microbial resources for the utilization of biomass wastes at low temperatures.

Hu H.,Beijing University of Chemical Technology | Song H.-Q.,Beijing University of Chemical Technology | Yu B.-R.,Beijing University of Chemical Technology | Cai Q.,Beijing University of Chemical Technology | And 2 more authors.
Polymer Chemistry | Year: 2015

The development of safe and effective supramolecular polycations has attracted much attention. In this work, a series of novel cyclodextrin (CD)-based supramolecular assemblies were readily prepared via the host-guest interaction by assembling different adamantane-functionalized α-CD derivatives with multiple β-CD-cored polycations. The supramolecular assemblies were investigated in terms of their pDNA-binding capabilities, cytotoxicities, cellular internalization and gene transfection efficiencies in HEK293, HepG2 and C6 cell lines. The supramolecular polycations displayed low cytotoxicities, similar to those of the CD-cored carriers. After self-assemblies, the gene transfection efficiencies and antitumor abilities of the supramolecular carriers were significantly enhanced. The present study demonstrates that such supramolecular preparation of CD-based polycations could provide a flexible strategy for the design and development of new assemblies with high efficiency and low cytotoxicity. This journal is © 2015 The Royal Society of Chemistry.

Zheng M.,Qiqihar University | Chi Y.,Northeast Forestry University | Yi H.,Chongqing Academy of Agricultural science | Shao S.,Qiqihar University
Biotechnology Letters | Year: 2014

A cDNA encoding for a laccase was isolated from the white-rot fungus Lenzites gibbosa by RT-PCR and expressed in the Pichia pastoris. The laccase native signal peptide efficiently directed the secretion of the recombinant laccase in an active form. Factors influencing laccase expression, such as pH, cultivation temperature, copper concentration and methanol concentration, were optimized. The recombinant enzyme was purified to electrophoretic homogeneity, and was estimated to have a MW of ~61.5 kDa. The purified enzyme behaved similarly to the native laccase produced by L. gibbosa and efficiently decolorized Alizarin Red, Neutral Red, Congo Red and Crystal Violet, without the addition of redox mediators. The decolorization capacity of this recombinant enzyme suggests that it could be a useful biocatalyst for the treatment of dye-containing effluents. This study is the first report on the synthetic dye decolorization by a recombinant L. gibbosa laccase. © 2013 Springer Science+Business Media Dordrecht.

PubMed | Chongqing Academy of Agricultural science and Southwest University
Type: Journal Article | Journal: Genes | Year: 2016

We identified a novel member of the metallothionein (MT) family,

Xia K.,CAS South China Botanical Garden | Liu T.,CAS South China Botanical Garden | Liu T.,University of Chinese Academy of Sciences | Ouyang J.,Chongqing Academy of Agricultural science | And 5 more authors.
DNA Research | Year: 2011

Autophagy is an intracellular degradation process for recycling macromolecules and organelles. It plays important roles in plant development and in response to nutritional demand, stress, and senescence. Organisms from yeast to plants contain many autophagy-associated genes (ATG). In this study, we found that a total of 33 ATG homologues exist in the rice [Oryza sativa L. (Os)] genome, which were classified into 13 ATG subfamilies. Six of them are alternatively spliced genes. Evolutional analysis showed that expansion of 10 OsATG homologues occurred via segmental duplication events and that the occurrence of these OsATG homologues within each subfamily was asynchronous. The Ka/Ks ratios suggested purifying selection for four duplicated OsATG homologues and positive selection for two. Calculating the dates of the duplication events indicated that all duplication events might have occurred after the origin of the grasses, from 21.43 to 66.77 million years ago. Semi-quantitative RTPCR analysis and mining the digital expression database of rice showed that all 33 OsATG homologues could be detected in at least one cell type of the various tissues under normal or stress growth conditions, but their expression was tightly regulated. The 10 duplicated genes showed expression divergence. The expression of most OsATG homologues was regulated by at least one treatment, including hormones, abiotic and biotic stresses, and nutrient limitation. The identification of OsATG homologues showing constitutive expression or responses to environmental stimuli provides new insights for in-depth characterization of selected genes of importance in rice. © 2011 The Author.

Zhang Y.,Chongqing University | Hu Z.,Chongqing University | Chu G.,Chongqing University | Huang C.,Chongqing University | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Eggplant (Solanum melongena L.) is an edible fruit vegetable cultivated and consumed worldwide. The purple eggplant is more eye-catching and popular for the health-promoting anthocyanins contained in the fruit skin. Two kinds of anthocyanin were separated and identified from purple cultivar (Zi Chang) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. To investigate the molecular mechanisms of anthocyanin accumulation in eggplant, the transcripts of anthocyanin biosynthetic and regulatory genes were analyzed in the fruit skin and the flesh of the purple cultivar and the white cultivar (Bai Xue). Compared with the other tissues, SmMYB1 and all anthocyanin biosynthetic genes except PAL were dramatically upregulated in the fruit skin of the purple cultivar. Overexpression of SmMYB1 activated abundant anthocyanin accumulation in the regenerating shoots of eggplant. These results prove that transcriptional activation of SmMYB1 accounts for constitutive upregulation of most anthocyanin biosynthetic genes and the onset of anthocyanin biosynthesis in the purple cultivar. © 2014 American Chemical Society.

Wang K.-J.,Chinese Academy of Agricultural Sciences | Li X.-H.,Chinese Academy of Agricultural Sciences | Zhang J.-J.,Chongqing Academy of Agricultural science | Chen H.,Chongqing Academy of Agricultural science | And 2 more authors.
Genetic Resources and Crop Evolution | Year: 2010

Introgression from soybean cultivars to its wild progenitor species is an interesting antidromic recombination in natural ecosystem with many consequences, including the alteration of genetic diversity, the origin of semi-wild soybean, and implication for biosafety of the wild progenitor species with future release of the genetically modified varieties. Although such interspecific introgression is not suspected to be ubiquitous in the sympatric regions of wild and cultivated soybeans, the documentations published based on some molecular experimental analyses on the introgression have been little substantiated by the occurring process morphologically and remain deficient for unquestionable evidence, owing to the lack of actual insight into the population dynamics. Here, we found the phenomenon of gene escape and presented the evidence for occurrence of introgression from soybeans into the wild species and for how originates about the semi-wild type soybean based on morphological investigation of population dynamics. Our results suggest that more attention should be paid to the escape of genetically modified genotypes to safeguard the biosafety of wild soybean gene pool, if GM soybeans are released in China, the place of origin of cultivated soybeans. © 2010 Springer Science+Business Media B.V.

Song J.,Chinese Academy of Agricultural Sciences | Gu J.,Chinese Academy of Agricultural Sciences | Zhai Y.,Chongqing Academy of Agricultural science | Wu W.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Bioresource Technology | Year: 2013

The fungal strain LZM1 was isolated from activated sludge and found to be capable of utilizing nicosulfuron as the sole nitrogen source for growth. Based on morphological and internal transcribed spacer evaluations, LZM1 was identified as a Talaromyces flavus strain. Under optimum conditions (pH 6.1, 29°C), T. flavus LZM1 degraded 100% of the initially added nicosulfuron (100mgL-1) within 5d. T. flavus LZM1 was also found to be highly efficient in degrading tribenuron methyl, chlorsulfuron, bensulfuron methyl, ethametsulfuron methyl, cinosulfuron, and rimsulfuron. Metabolites from nicosulfuron degradation were identified by liquid chromatography mass spectrometry, and a possible degradation pathway was deduced. These results show that T. flavus LZM1 may possess potential to be used in bioremediation of nicosulfuron-contaminated environments. © 2013 Elsevier Ltd.

Sun G.-M.,Jinan University | Yang P.-H.,Jinan University | Sun J.-H.,Chongqing Academy of Agricultural science | Cai J.-Y.,Jinan University
Faguang Xuebao/Chinese Journal of Luminescence | Year: 2011

Due to the unique and tunable optical properties, gold nanorods have potential and important applications in the biomedical field. In this paper, gold nanorods with the aspect ratio 8 were prepared, an outstanding fluorescence feature of the gold nanorods excited at 480 nm was observed in terms of a strong fluorescence emission at 707 nm and a relatively weak fluorescence at 560 nm. Based on their fluorescence properties, HepG2 cells were labeled by this type of gold nanorods, and were studied by using laser scanning confocal microscope. Green and red fluorescence images of gold nanorods were collected under the excitation of 488 nm. Furthermore, high-resolution atomic force microscopy was used to obtain the morphology and mechanical properties changes of the HepGz cells.

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