Song Q.-Z.,Chonggang General Hospital |
Li T.,Chonggang General Hospital |
Feng C.-B.,Chonggang General Hospital |
Lin Y.-J.,Chonggang General Hospital |
Hong H.,Chonggang General Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014
Background: The study on polymethylmethacrylate effects on the cardiovascular system during percutaneous vertebroplasty is limited. Objective: To estimate the effects of polymethylmethacrylate on the cardiovascular system during percutaneous vertebroplasty. Methods: From July 2011 to September 2013, a total of 104 patients (167 vertebral bodies) with osteoporotic vertebral compression fractures and without serious cardiovascular related basic diseases were enrolled in this retrospective study. Mean arterial pressure, heart rate and oxygen saturation were recorded at the following time points: before local anesthesia, before polymethylmethacrylate injection, 0, 3, 5 and 10 minutes after polymethylmethacrylate injection. Results And Conclusion: The mean arterial pressure was decreased after polymethylmethacrylate injection at 0, 5 minutes (P < 0. 05) and 3 minutes (P < 0. 01), and returned to the pre-injection level at 10 minutes. At the same time, the heart rate was increased after polymethylmethacrylate injection at 0, 5 minutes (P < 0. 05) and 3 minutes (P < 0. 01), and returned to the pre-injection level at 10 minutes. Oxygen saturation was over 95%, which was unchanged before and after polymethylmethacrylate injection. The results indicated that polymethylmethacrylate injection has a transient effect on cardiovascular system during percutaneous vertebroplasty.
Lai X.-F.,Chongqing Medical University |
Lai X.-F.,ChongGang General Hospital |
Qin H.-D.,Chongqing Medical University |
Guo L.-L.,Chongqing Medical University |
And 4 more authors.
Cellular Physiology and Biochemistry | Year: 2014
Aims: Neutrophils can synthesize leukotriene B4 (LTB4) by activating the 5-lipoxygenase (5-LO)signaling pathway. LTB4 is a pro-inflammatory mediator associated with the etiology and progression of atherosclerosis. It can increase function and number of neutrophils in an autocrine manner. Since hypercholesterolemia is associated with an increase in the number and function of neutrophils, we hypothesized that this effect could be mediated through increased production of LTB4 in neutrophils.Methods/Results: Hypercholesterolemia was modeled in Wistar rats by feeding them with a high cholesterol diet. The induction of hypercholesterolemia caused an increase in the plasma levels of LTB4, following lipopolysaccharide stimulation. This effect was recapitulated in vitro, both in the presence and absence of stimulation with the activator of 5-LO, A23187. Neutrophils in hypercholesterolemia rats expressed similar total levels of 5-LO as control rats, but displayed increased nuclear localization of 5-LO, as well as elevated levels of phosphorylated 5-LO and ERK1/2. In vitro, MβCD/cholesterol complexes enriched cholesterol in neutrophils, resulted in similar changes in 5-LO/LTB4. In addition, these alterations could be inhibited with the ERK inhibitor PD98059.Conclusion: Hypercholesterolemia increases LTB4 production in neutrophils by increasing the nuclear localization of 5-LO, which is the result of its phosphorylation by activated ERK1/2. Copyright © 2014 S. Karger AG, Base.