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Sharma M.C.,Devi Ahilya University | Sharma S.,Chodhary Dilip Singh Kanya Mahavidyalya
International Journal of ChemTech Research | Year: 2011

Ultraviolet absorption spectrophotometric method for the estimation of poorly water soluble drug like Lamivudine in pharmaceutical formulations has been developed. Aqueous solubility of this selected model drug was to a great extent (21 to 243fold) in 5.0 M sodium benzoate. Lamivudine shows maximum absorbance at 315 nm. Beer's law was obeyed in the concentration. Results of analysis were validated statistically and by recovery studies. Source


Sharma S.,Chodhary Dilip Singh Kanya Mahavidyalya | Sharma M.C.,Devi Ahilya University | Sahu N.K.,Dr Hari Singh Gour University
Arabian Journal of Chemistry | Year: 2012

Simple, precise, and accurate UV-Spectrophotometric and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous determination of Nitazoxanide and Ofloxacin in pharmaceutical preparations have been developed and validated. The method was developed using aluminum plates pre-coated with silica gel 60 F 254 HPTLC plates as a stationary phase with toluene:chloroform:carbon tetra chloride:toluene:glacial acetic acid solutions in the proportion of (10:5:3:0.5 v/v/v/v) as mobile phase. Densitometric quantification was performed at 241 nm. Well-resolved bands were obtained with R F values 0.36, 0.57 and 0.63 for Rosiglitazone maleate, Nitazoxanide, and Ofloxacin, respectively. Rosiglitazone maleate was used as an internal standard. The calibration curves were linear within the concentration range of 5-25 μg/ml for each drug. Two simple spectrophotometric methods have been developed for simultaneous estimation of Nitazoxanide, and Ofloxacin from tablet dosage form. The first method, simultaneous equation method, involves the measurement of absorbances at two wavelengths 221.8 nm (λ max of Nitazoxanide) and 244.3 nm (λ max of Ofloxacin), and the second method is First order derivative spectroscopy, wavelengths selected for quantitation were 263.6 nm for Nitazoxanide and 269.2 nm for Ofloxacin. The proposed method gave good validation results and the statistical analysis performed proved that the method is precise, accurate and reproducible, and hence can be employed for routine analysis of Nitazoxanide and Ofloxacin in bulk and commercial formulations. © 2012. Source


Sharma S.,Chodhary Dilip Singh Kanya Mahavidyalya | Sharma M.C.,Devi Ahilya University
Arabian Journal of Chemistry | Year: 2012

The study is focused to develop and validate a UV-Spectrophotometric and Densitometry method for simultaneous estimation of Irinotecan from their dosage form. Based on spectrophotometric characteristic of method are described for the simultaneous determination of Irinotecan HCl, at 247 nm for simple UV spectrum and at 268 nm for derivative spectrum was found adequate for quantification. The method was validated for linearity, accuracy, precision, reproducibility, and specificity as per International Conference on Harmonization guidelines. The linearity signal and concentration of Irinotecan in the range of 2-10 μg/ml in aqueous solution present a correlation coefficient (r 2) of 0.9999 for simple UV and 0.9997 for first order derivative spectrum. Second method is the high performance thin layer chromatographic (HPTLC) separation followed by densitometric measurements on normal phase silica gel 60F 254. The chromatographic separation was carried out on precoated silica gel 60F 254 aluminium plates using a mixture of toluene/ethyl acetate/methanol/carbon tetrachloride, in the volume ratio of 9.2:5:0.9:0.8 (v/v/v/v) respectively as mobile phase. Densitometric analysis was carried out at 317 nm. The linear regression analysis data showed good linear relationship in the concentration range of 200-1200 (ng/band) for Irinotecan. The limits of quantitation for Irinotecan were 34 (ng/band). The limit of detection (LOD) and limit of quantitation (LOQ) were 36 and 57 ng/spot, respectively. The drug was satisfactorily resolved with R f value 0.34 ± 0.08. The accuracy and repeatability of the proposed method were ascertained by evaluating various validation parameters like linearity (200-1200 ng/spot). The method was found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of Irinotecan bulk and marketed dosage form. © 2012. Source


Sharma S.,Chodhary Dilip Singh Kanya Mahavidyalya | Sharma M.C.,Devi Ahilya University
Arabian Journal of Chemistry | Year: 2012

A rapid and precise method (in accordance with ICH guidelines) is developed for the quantitative simultaneous determination of Drotaverine hydrochloride and Omeprazole in a combined pharmaceutical dosage form. Three methods are described for the simultaneous determination of Drotaverine hydrochloride and Omeprazole in a binary mixture. The first method was based on UV-Spectrophotometric determination of two drugs, using Vierordt !s simultaneous equation method. It involves absorbance measurement at 226.8 nm (λ max of Drotaverine hydrochloride) and 269.4 nm (λ max of Omeprazole) in methanol; linearity was obtained in the range of 5-30 μg ml -1 for both the drugs. The second method was based on HPLC separation of the two drugs using potassium dihydrogen phosphate buffer pH 5.0: Acetonitrile: Triethylamine (60:40:0.5, v/v) as a mobile phase. Areas were recorded at 260 nm for both the drugs and retention time was found to be 2.71 min. and 3.87 min for Drotaverine hydrochloride and Omeprazole, respectively at 1.0 mL/min flow rate. The selected chromatographic conditions were found to determine Drotaverine hydrochloride and Omeprazole quantitatively in a combined dosage form without any physical separation. The method has been validated for linearity, accuracy and precision. Linearity was found over the range of 5-30 μg mL -1 for both drugs. The third method was based on HPTLC method for simultaneous quantification of these compounds in pharmaceutical dosage forms. Precoated silica gel 60 F 254 plate was used as stationary phase. The separation was carried out using Glacial acetic acid:Cyclohexane:Methanol:(80:15:5 v/v/v) as mobile phase. The proposed method was found to be fast, accurate, precise, reproducible and rugged and can be used for a simultaneous analysis of these drugs in combined formulations. © 2012. Source


Sharma M.C.,Devi Ahilya University | Sharma S.,Chodhary Dilip Singh Kanya Mahavidyalya
International Journal of ChemTech Research | Year: 2011

A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of Repaglinide in pure and tablet forms. The drug was found almost stable to neutral and photolytic condition. Resolution of drug and the degradation products formed under different stress conditions were successfully achieved on a Luna C18 (5μm×25cm×4.6mm) column utilizing mixture of Acetonitrile and potassium dihydrogen phosphate buffer (pH 4.5 adjusted with orthophosphoric acid) in the ratio of 60:40 (v/v) as mobile phase at a flow rate of 1.0 ml min-1 and detection was performed at 278 nm. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. Source

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