Cha W.-S.,Chosun University |
Park S.-S.,Dongguk University |
Kim S.-J.,Chosun University |
Choi D.,Chosun University |
Choi D.,Chodang University
Bioresource Technology | Year: 2010
Biochemical and enzymatic properties of a fibrinolytic enzyme purified from Pleurotus eryngii cultivated under solid-state conditions using corn cob as energy source were investigated. The molecular mass of the enzyme was estimated to be 14 kDa by SDS-PAGE. The enzyme exhibited the highest activity (28.96 mol/min/mg) for the substrate tosyl-Gly-Pro-Lys-p-nitroanilide. Km and Vmax values were 0.18 mM and 53.5 U/ml, respectively. The enzyme was completely inhibited by 1.0 mM phenylmethylsulfonyl fluoride (PMSF). The N-terminal sequence was A-M-D-S-Q-T-D-A-S-Y-G-LA-N-D. This sequence exhibited a high degree of similarity to the N-terminal sequences of the subtilisin-like serine proteases. The enzyme was very stable at pH 4.0-6.0 with an optimum pH 5.0 at 40 °C. The enzyme rapidly hydrolyzed the A α-chain of fibrinogen within 5 min of incubation, followed by the B β-chain after 10 min. The fibrinolytic enzyme from P. eryngii cultivated under solid-state conditions using corn cob could be potentially exploited in thrombolytic therapy. © 2010 Elsevier Ltd. All rights reserved.
Kim J.,Korea Institute of Toxicology |
Kim J.,Korea University |
Kang C.-M.,Korea Institute of Toxicology |
Kang C.-M.,Chodang University
Bioresource Technology | Year: 2015
The co-digestion of multiple substrates is a promising method to increase methane production during anaerobic digestion. However, limited reliable data are available on the anaerobic co-digestion of food waste leachate with microalgal biomass. This report evaluated methane production by the anaerobic co-digestion of different mixtures of food waste leachate, algal biomass, and raw sludge. Co-digestion of substrate mixture containing equal amounts of three substrates had higher methane production than anaerobic digestion of individual substrates. This was possibly due to a proliferation of methanogens over the entire digestion period induced by multistage digestion of different substrates with different degrees of degradability. Thus, the co-digestion of food waste, microalgal biomass, and raw sludge appears to be a feasible and efficient method for energy conversion from waste resources. © 2015 Elsevier Ltd.
Lee C.-K.,Chodang University |
Choi J.-S.,Chosun University
Pharmacology | Year: 2010
Silibinin, a flavonoid, is an inhibitor of P-glycoprotein (P-gp)-mediated efflux transporters, and its oxidative metabolism is catalyzed by CYP3A4. The purpose of this study was to investigate the effect of oral silibinin on the bioavailability and pharmacokinetics of orally and intravenously administered paclitaxel in rats. The pharmacokinetic parameters of paclitaxel were determined in rats after oral (40 mg/kg) or intravenous (4 mg/kg) administration in the presence and absence of silibinin (0.5, 2.5 or 10 mg/kg). The effect of silibinin on the P-gp as well as CYP3A4 activity was also evaluated. Silibinin inhibited CYP3A4 enzyme activity with an IC50 of 1.8 μmol/l. In addition, silibinin significantly inhibited P-gp activity. Compared to the control group, silibinin significantly (p < 0.05 by 2.5 mg/kg, p < 0.01 by 10 mg/kg) increased the area under the plasma concentration-time curve (65.8-101.7% higher) of oral paclitaxel. Silibinin also significantly increased (p < 0.05 by 2.5 mg/kg, 31.0% higher; p < 0.01 by 10 mg/kg, 52.9% higher) the peak plasma concentration of paclitaxel. Consequently, the absolute bioavailability of paclitaxel was increased by silibinin compared to that in the control group, and the relative bioavailability of oral paclitaxel was increased 1.15- to 2.02-fold. The intravenous pharmacokinetics of paclitaxel were not affected by the concurrent use of silibinin in contrast to the oral administration of paclitaxel. Accordingly, the enhanced oral bioavailability in the presence of silibinin could mainly be due to the increased intestinal absorption of paclitaxel via P-gp inhibition. Copyright © 2010 S. Karger AG.
Lee C.-K.,Chodang University |
Ki S.-H.,Chosun University |
Choi J.-S.,Chosun University
Biopharmaceutics and Drug Disposition | Year: 2011
This study aimed to investigate the effects of oral curcumin on the pharmacokinetics of intravenous and oral etoposide in rats. Intravenous (6 mg/kg) or oral (2 mg/kg) etoposide was administered to rats in the absence and the presence of oral curcumin (0.4, 2 or 8 mg/kg). The effects of curcumin on the P-glycoprotein (P-gp) and CYP3A4 activity was also evaluated. Curcumin inhibited CYP3A4 enzyme activity with a 50% inhibition concentration (IC 50) of 2.7 μM. In addition, curcumin (10 μm) significantly enhanced the cellular accumulation of rhodamine-123 in MCF-7/ADR cells overexpressing P-gp. Compared with the control group (given etoposide alone), curcumin (2 or 8 mg/kg) increased significantly the oral bioavailability (AUC and C max) of etoposide. Consequently, the extent of absolute oral bioavailability (F) of etoposide with curcumin was significantly enhanced compared with that in the control group. In contrast, curcumin did not affect the pharmacokinetics of etoposide after intravenous administration. Therefore, the enhanced oral bioavailability of etoposide in the presence of curcumin might be due mainly to inhibition of the P-gp efflux pump in the small intestine and possibly by reduced first-pass metabolism of etoposide in the small intestine by inhibition of CYP3A activity in rats. The combined use of curcumin may be helpful to improve the F of etoposide in chemotherapeutic applications. Copyright © 2011 John Wiley & Sons, Ltd.
Chun C.H.,Wonkwang University |
Kim J.W.,Wonkwang University |
Kim S.H.,Wonkwang University |
Kim B.G.,Wonkwang University |
And 2 more authors.
Knee | Year: 2014
Background: Proper treatment of bone loss is essential for the long term durability of revision TKA. However, the method of choice in managing large bone defects is still under debate. We therefore assessed the mid to long term clinical and radiographic results of revision TKA using a fresh frozen femoral head allograft and a standard condylar implant or varus-valgus constrained prosthesis with a diaphyseal-engaging stem. Methods: We retrospectively reviewed the records of 27 patients who had undergone revision TKA between August 1997 and March 2003 using a fresh frozen femoral head allograft and a standard condylar implant or varus-valgus constrained prosthesis with a diaphyseal-engaging stem. The median follow-up period was 107. months (range, 96-157. months). Results: Clinical evaluation revealed that the mean range of motion had increased from 71° to 113° and the mean Hospital for Special Surgery knee score had improved from 46 to 83 points. The overall tibio-femoral angle improved from varus 7.3° to valgus 6.l°. In 26 out of 27 knees, union was demonstrated at an average of seven months postoperatively, and there were no cases of collapse, disease transmission or stress fractures. In one knee, an infection recurred. Conclusions: Our results demonstrate that femoral head allografts in treatment of severe bone defects are reliable and durable. If possible, less constrained prostheses with diaphyseal-engaging stems should be chosen for increased durability. Level of evidence: Level IV, therapeutic study. See Guidelines for Authors for a complete description of levels of evidence. © 2013.
Kim S.H.,Wonkwang University |
Chun C.H.,Wonkwang University |
Chun K.C.,Wonkwang University |
Jo H.J.,Wonkwang University |
Kim K.M.,Chodang University
American Journal of Sports Medicine | Year: 2012
Background: There is a lack of histological studies investigating the presence of mechanoreceptors in anterior cruciate ligament (ACL) allografts. Hypothesis: Mechanoreceptors would not grow in Achilles allografts after ACL reconstruction. Study Design: Case series study; Level of evidence, 4. Methods: Tissue samples were obtained from 11 patients who underwent ACL reconstruction using Achilles tendon allografts. They underwent biopsies during second-look arthroscopies. The mean period from ACL reconstruction to harvesting tissue was 26.63 months (range, 12-120 months). The control group consisted of 2 normal ACLs procured from 42- and 45-year-old men who underwent amputation above the knee due to trauma. Results: Ruffini corpuscles and free nerve endings were shown to be present in the specimens of the control group by prcessing hematoxylin-eosin stains and immunohistochemical stains with monoclonal antibodies against S-100. In the Achilles allografts, mechanoreceptors were not observed. However, fibroblasts, collagen fibers, and vessels that were not present in fresh-frozen Achilles allografts before surgery were observed. Conclusion: The results demonstrate that Achilles tendon allografts appeared similar to normal ligaments except for the lack of histological evidence of mechanoreceptors. In other words, there are no newly ingrown mechanoreceptors in ACL allografts. © 2012 The Author(s).
Kim T.-H.,Korea Institute of Oriental Medicine |
Lim H.-J.,Chodang University |
Kim M.-S.,Korea Food Research Institute |
Lee M.S.,Korea Institute of Oriental Medicine
Maturitas | Year: 2012
Benign prostatic hyperplasia (BPH) is a common chronic condition in older men. The aim of this overview of systematic reviews (SRs) is to summarise the current evidence on the efficacy and adverse effects of dietary supplements for treating BPH with lower urinary tract symptoms. We searched 5 electronic databases and relevant overviews without limitations on language or publication status. Six SRs of 195 articles were included in this overview. Serenoa repens was reviewed in 3 studies and no specific effect on BPH symptoms and urinary flow measures was observed. However, β-sitosterol, Pygeum africannum and Cernilton were reviewed in one study each, and significant improvement was observed for all three. All the included compounds have mild and infrequent adverse effects. SRs on β-sitosterol, Pygeum africannum and Cernilton have not been updated since 2000, thus an update of reviews on these compounds will be necessary in the future. © 2012 Elsevier Ireland Ltd.
Chung D.-O.,Chodang University
Journal of the Korean Society of Food Science and Nutrition | Year: 2010
This study was conducted to investigate the quality characteristics of tofu (soybean curd) prepared with the addition of Enteromorpha intenstinalis powder (EIP). The moisture, crude ash, carbohydrate, crude protein, and crude lipid contents of the EIP were 6.04%, 24.09%, 39.68%, 28.25%, and 1.94%, respectively. The yield rate of the tofu did not differ significantly according to the level of added EIP, however there was a significant decrease in pH and a significant increase in acidity. The L and a values of the tofu color were decreased as the amount of EIP in the formulation increased, whereas the b values was increased. Furthermore, hardness and brittleness were significantly increased as the level of EIP increased. In terms of overall acceptability, the preferred tofu samples were the control and that of tofu mixed with 0.2% EIP addition group.
Bae H.-S.,Chodang University
Journal of the Korean Society of Food Science and Nutrition | Year: 2013
This study was carried out to investigate the levels of awareness of fermented vegetable beverages according to consumers' gender. The data were obtained through questionnaires completed randomly by 441 respondents and analyzed using the SPSS, t-tests, ANOVA, X-square tests, multiple regression, and logistic regression analyses. Among 441 respondents, 32.9% of male and 30.2% of female ever consumed fermented vegetable beverages. This beverage were consumed as an alternative for water in males (6.3%) and in females (7.9%) which was the most common reason for consumption. As for the most common reason for non-consumption, males (5.9%) and females (6.6%) responded that they did not consume fermented vegetable beverages because people around them have never consumed fermented vegetable beverages. The awareness that fermented vegeta-ble beverages help reduce hunger was higher in females (3.2%) than and in males (2.9%). These results revealed that the awareness of fermented vegetable beverages as hunger alleviators was more prevalent in females than in males which can be used as preliminary data for research on the development of fermented vegetable beverages.
Park G.,Chodang University
International Journal of Bio-Science and Bio-Technology | Year: 2016
The purpose of the study is to assess the effect of basic CPR training by 2010 AHA guideline in nursing students in order to provide nursing students with the basic data on CPR training. The study features a pre-experiment that uses a before-after design in a single group. The study selected 102 nursing students who participated in training pro-grams conducted in the CPR Center of the C University located in G City from Jan. - Sept. 2013. The study used a knowledge measurement tool that was modified by the re-searcher based on the 2010 AHA guideline and Park Jeong-mi's tool (2006), and the tools of Kang Kyung-hui (2004) and Park So-hyun (2002) were modified by the re-searcher and used for attitude measurement. For self-efficacy measurement, the study used a tool modified by Park Jeong-mi (2006), which modified the tools of Kang Kyung-hui (2004) and Schlesse, et al., (1995). The study used the SPSS WIN 19.0 program for data analysis to calculate technical statistics for general characteristics, CPR knowledge, attitude, and self-efficacy of participants, and conducted a paired t-test to verify differences in general characteristics, CPR knowledge, attitude, and self-efficacy, and a paired t-test to verify differences in CPR knowledge, attitude, and self-efficacy before and after training. For correlation analysis between CPR knowledge, attitude, and self-efficacy of study participants, the study used Pearson's correlation coefficients. The study showed that the basic CPR training for nursing students significantly im-proved knowledge, attitude, and self-efficacy in relation to CPR, and the correlation between the variable results suggests a net significant correlation between CPR attitude and self-efficacy only. © 2016 SERSC