Chitose, Japan
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Imura Y.,Osaka University | Naka N.,Osaka University | Naka N.,Japan National Cardiovascular Center Research Institute | Outani H.,Osaka University | And 9 more authors.
BMC Research Notes | Year: 2013

Abstract. Background: Whereas we can use several human epithelioid sarcoma (ES) cell lines for basic and preclinical research, an angiomatoid ES cell line has not been reported to date. We have treated a case of an angiomatoid ES developing in the right upper extremity of a 67-year-old man. Methods. An angiomatoid ES cell line, Asra-EPS was newly established and characterized for its morphology, growth rate and chromosomal analysis. Tumorigenicity of Asra-EPS cells was also analyzed in athymic nude mice. Results: Asra-EPS cells were round, polygonal or spindle-shaped with an abundant cytoplasm and have been maintained continuously in vitro for over 150 passages during more than 15 months. These cells secreted cancer antigen 125 (CA 125), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) into the culture medium. Asra-EPS cells were tumorigenic when implanted in nude mice with tumors reaching a volume of 1000 mm3 at around 50 days. Histological features of tumors formed in mice were essentially the same as those of the original tumor, exhibiting a multinodular proliferation of eosinophilic epithelioid and spindle-shaped cells with prominent areas of hemorrhage and blood-filled cystic spaces strikingly corresponding to the potential of hemorrhagic cyst formation in the original tumor. They showed immunopositive staining for cytokeratins (AE1/AE3 and CAM5.2), epithelial membrane antigen (EMA), vimentin, CD31, CD34 and CA 125, but negative for integrase interactor 1 (INI-1) and factor VIII-related antigen. Conclusions: The established cell line represents a biologically relevant new tool to investigate the molecular pathology of human angiomatoid ES and to evaluate the efficacy of novel therapeutics both in vitro and in vivo. © 2013 Imura et al.; licensee BioMed Central Ltd.


Emori M.,Sapporo Medical University | Kaya M.,Chitose City Hospital | Sasaki M.,Sapporo Medical University | Wada T.,Sapporo Medical University | And 2 more authors.
Japanese Journal of Clinical Oncology | Year: 2012

The management of giant cell tumor of the proximal humerus that extends to the joint is challenging. Here, we report a case of proximal humerus giant cell tumor with cortical bone destruction extending to the shoulder joint. Pre-operative selective arterial embolization induced peripheral tumor ossification. Subsequently, the lesion was removed by intralesional curettage, and the cavity was filled with cement. Macroscopically, the inner wall of the cavity was found to be lined with a thick fibrous membrane. Histologically, massive fibrosis and resultant remodeling of the destroyed cortical bone were induced, which was consistent with the peripheral ossification on the plain radiograph. We believe that selective arterial embolization can be an effective neoadjuvant therapy for giant cell tumors of the extremities, especially for tumors with large cortical defects or joint involvement. © The Author 2012. Published by Oxford University Press. All rights reserved.


Saito H.,Hokkaido University | Nakayama N.,Hokkaido University | Takikawa S.,Chitose City Hospital | Ushikoshi S.,Hokkaido Medical Center | And 3 more authors.
Neurological Surgery | Year: 2012

Isolated abducens nerve palsies associated with the rupture of intracranial aneurysms have rarely been reported. We report two cases of isolated bilateral abducens nerve palsies occurring after subarachnoid hemorrhage due to the rupture of an intracranial aneurysm. Case 1 : A 49-year-old woman had bilateral abducens nerve palsies following subarachnoid hemorrhage due to the rupture of the left vertebral artery-posterior inferior cerebellar artery aneurysm. Case 2: A 55-year-old man had bilateral abducens nerve palsies following subarachnoid hemorrhage due to dissecting aneurysm of the right vertebral artery. Case 1 and 2 were treated with surgical clipping of the aneurysm and internal occlusion of the parent artery. In both cases, bilateral abducens nerve palsies achieved almost full recovery several months after treatment. It is speculated that the main causes of palsies are compression and stretching of the bilateral abducens nerves by a thick clot in the prepontine cistern. Although most of the abducens palsies may be reversible and have good prognosis, it is important that they are kept in mind as isolated symptoms of subarachnoid hemorrhage.


Kaya M.,Chitose City Hospital | Wada T.,Sapporo Medical | Nagoya S.,Sapporo Medical | Yamashita T.,Sapporo Medical
Journal of Orthopaedic Science | Year: 2011

Background: Myxofibrosarcoma is characterized by a high local recurrence rate despite optimal surgical treatment. The definition of prognostic factors for recurrence offers high-risk patients a closer follow-up and a multidisciplinary therapeutic approach. Methods: A cohort of 23 patients treated for primary myxofibrosarcoma was retrospectively analyzed. The patients (sex and age), tumors (size, stage, tumor location, bone and/or joint attachment), radiological findings (abnormal signal extension in MRI), histological findings (FNCLCC grade and microscopic extension along the muscle fascia), and treatment (surgical margin) characteristics were included in univariate prognostic factor analysis. Results: After a median follow-up of 63.3 months (range 15-191), the overall recurrence rate was 34.7%. Median time between initial surgery and recurrence was 24.8 months (range 8-52). Inadequate surgical margins (p = 0.026) and bone and/or joint attachment (p = 0.001) were associated with an increased recurrence rate. Conclusion: For the further improvement of local recurrence-free survival of patients with myxofibrosarcoma, accurate diagnosis of the tumor extension and adequate planning for the surgical margin should be focused on in cases with bone and/or joint attachment. © 2011 The Japanese Orthopaedic Association.


Sato Y.,Sapporo Medical University | Takayama T.,Tokushima University | Sagawa T.,Sapporo Medical University | Takahashi Y.,Hokkaido Cancer Center | And 15 more authors.
Cancer Chemotherapy and Pharmacology | Year: 2010

Purpose: We evaluated the activity and toxicity of docetaxel, cisplatin, and S-1 (DCS) combination chemotherapy in patients with unresectable metastatic gastric cancer. Methods: Patients with histologically proven, unresectable metastatic gastric adenocarcinoma, performance status (PS) 0-2, and no prior chemotherapy were eligible. Patients received oral S-1 (40 mg/m2 b.i.d.) on days 1-14 and intravenous cisplatin (60 mg/m2) and docetaxel (60 mg/m2) on day 8 every 3 weeks. Results: Thirty-four patients were enrolled between March 2005 and April 2007. Three patients were considered ineligible and did not receive the DSC therapy. Clinical characteristics were as follows: median age, 63 years (range, 44-77); PS, 0/1/2:23/8/0; women/men, 8/23; and well-differentiated/undifferentiated adenocarcinoma, 10/21. The objective response rate was 87.1% with 1 complete response (3.2%) and 26 partial responses (83.9%) in 31 assessable patients. Four had stable disease (12.9%) but none had progressive disease. Of these 27 responders, 8 (25.8%) achieved downstaging and 7 (22.6%) underwent curative surgery. The median survival time and progression-free survival were 687 days [confidence interval (95% CI), 600.0-1,138.1] and 226 days (95% CI, 182.5-379.3), respectively. Most common grade 3/4 hematologic toxicity was neutropenia (77.4%). Most common grade 3 nonhematologic toxicities included anorexia (35.5%) and nausea (32.3%). All treatment-related toxicities resolved, and no toxic deaths were observed. Conclusions: DCS combination chemotherapy is highly active against unresectable metastatic gastric cancer and can be given safely with proper management of adverse events. Further studies of this combination are warranted. © Springer-Verlag 2009.


Hamada K.,Chitose City Hospital | Onuma N.,Chitose City Hospital
Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society | Year: 2010

An 82-year-old woman was admitted because of progressing cough and dyspnea. Two years prior to admission, she had undergone an operation for extramammary Paget's disease which resected the primary lesion, the left labium majora, and ipsilateral inguinal and obturator lymph nodes. Thoracic images showed diffuse infiltrative shadows and bilateral effusions. Repeated thoracenteses revealed no microbes or malignant cells. Antibiotic treatment was ineffective. Transbronchial lung biopsy specimens revealed Paget cells, and immunohistochemical stains confirmed pulmonary metastases of Paget's disease. Cytokeratin 19 fragments were positive in the serum, pleural effusion, and lung biopsy specimens. The patient died of respiratory failure 3 years after the initial diagnosis of extramammary Paget's disease.


We investigated whether objective evaluation by using the palliative prognostic index(PPI), controlling nutritional status(COUNT), and prognostic nutritional index(PNI)can provide prognostic information during the transition from chemotherapy to palliative care in patients with advanced or recurrent gastrointestinal cancer.The subjects were 28 patients with gastrointestinal cancer who died of their disease between January 2009 and June 2012. We compared the PPI, COUNT, and PNI scores between patients who died within 90 days of completing chemotherapy(Group A, n=14)and patients who survived for 90 or more days(Group B, n=14).The PPI score for Group A(4.0)was significantly higher than that for Group B(0.8)(p<0.001). The COUNT score was also significantly higher for Group A(6.3)than for Group B (3.9)(p=0.033). A significant difference in survival was evident when the cutoff value for PNI was set at 40 in the critical region(68/118, p=0.04).Our study suggests that the PPI, COUNT, and PNI may be useful for objective evaluation during the transition from chemotherapy to palliative care.


PubMed | Chitose City Hospital
Type: Case Reports | Journal: Masui. The Japanese journal of anesthesiology | Year: 2010

An 83-year-old woman had felt malaise. Blood examination showed renal failure, anemia, and hypoproteinemia. On the 7th day, she suddenly had tarry stool. But, gastroscopy and colonfiberscopy disclosed no remarkable abnormality on the mucosal surface of the stomach, the duodenum and the large intestine. On the 10th day, her symptom became aggravated, and emergency laparotomy and endoscopy in the small intestine was scheduled. After the jejunotomy, she underwent endoscopy of the small intestine. The endoscopic examination showed multiple ulcer of the jejunum, and she underwent jejunectomy. Pathologic examination diagnosed her as having non specific multiple ulcer of the small intestine with jejunal lesion.


PubMed | Chitose City Hospital
Type: Case Reports | Journal: Masui. The Japanese journal of anesthesiology | Year: 2010

We report successful anesthetic management of elective cesarean section in a 30-year-old patient with Basedow disease using landiolol hydrochloride, a short-acting beta-1 adrenergic blocker of which greatest advantages are its short duration of action, rapid clearance and high beta-1 selectivity. The patient received landiolol for the prevention of aggravated tachycardia and tachyarrhythmia during cesarean section. She received landiolol continuous infusion at a rate of 5 microg x kg x min(-1) to 8 microg x kg x min(-1) until the uneventful delivery of the infant with good Apgar score, under combined spinal epidural anesthesia (CESA). We could maintain maternal hemodynamics stable leading to good post-cesarean uterus contraction. No severe adverse effects were observed in the infant. In conculusion, landiolol is useful for maintaining the hemodynamics stable in a pregnant woman with Basedow disease and keeping the infant safe from any adverse effect by infusion of landiolol.


PubMed | Chitose City Hospital
Type: Case Reports | Journal: Nihon Kokyuki Gakkai zasshi = the journal of the Japanese Respiratory Society | Year: 2010

An 82-year-old woman was admitted because of progressing cough and dyspnea. Two years prior to admission, she had undergone an operation for extramammary Pagets disease which resected the primary lesion, the left labium majora, and ipsilateral inguinal and obturator lymph nodes. Thoracic images showed diffuse infiltrative shadows and bilateral effusions. Repeated thoracenteses revealed no microbes or malignant cells. Antibiotic treatment was ineffective. Transbronchial lung biopsy specimens revealed Paget cells, and immunohistochemical stains confirmed pulmonary metastases of Pagets disease. Cytokeratin 19 fragments were positive in the serum, pleural effusion, and lung biopsy specimens. The patient died of respiratory failure 3 years after the initial diagnosis of extramammary Pagets disease.

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