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CAMBRIDGE, United Kingdom

Agency: GTR | Branch: Innovate UK | Program: | Phase: Feasibility Study | Award Amount: 135.62K | Year: 2014

The purpose of the EDAM project is to test the feasibility of combining two well established technologies in order to develop an innovative process for the production and isolation of amino acids. Biocatalysis is a well established technology in the chemical and pharmaceutical industries, allowing the conversion of an amino acid precursor into a target amino acid using 1 or 2 enzymes. Electrodialysis membranes have been used for many years in the water purification industry, however wider applications of this technology remain under utilised. We propose to utilise a two enzyme reaction system to facilitate the production of amino acids. Subsequent application of a direct current electric field to the reaction mixture will then lead to electromigration of the charged amino acid species across an ion exchange membrane and product purification.

Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: KBBE-2009-3-3-02 | Award Amount: 3.47M | Year: 2010

This project intends to engineer transaminase libraries that will be applied as the main enzymatic technology to deliver the amine functionality in the commercially valuable products of both chiral and bulk amine targets. These enzymes will be used in enzymatic cascades where simple starting materials are converted into the required intermediates for transamination or further enzymatic steps will be used to remove products from the transaminase reaction which will add value by extra functionality. This will also be supported by the development of enzymatic cascades to deliver efficient co-factor recycling and achieve the high conversions required for industrial use. A high throughput screening method based on a further enzymatic cascade will be developed. Engineering solutions will be used to overcome obstacles associated with the implementation of this core technology on a larger scale and integrate the use of other enzymes into the synthetic pathway to allow multi-step, multi-enzyme cascades to be used to deliver complex multi-functional amine products and processes. The industrial partner will target the development of enzymes from the project for specific application into their new products range. Life cycle analysis and environmental impact analysis will compare the final methods with conventional chemical synthesis and allow advantages to be objectively defined.

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