Chen Y.,National Defense Medical Center |
Yang S.-H.,National Taiwan University Hospital |
Hueng D.-Y.,Tri Service General Hospital |
Syu J.-P.,National Taiwan University |
And 2 more authors.
Chemico-Biological Interactions | Year: 2014
Cordycepin, 3′-deoxyadenosine from Cordyceps sinensis, has been shown to exert anti-tumor effects in several cancer cell lines. This study investigated the effect of cordycepin on a rat glioma cell line. Cordycepin caused apoptosis in C6 glioma cells in a time- and concentration-dependent manner, but did not affect the survival of primary cultured rat astrocytes. Cordycepin increased the total protein levels of p53 and phosphorylated p53 in the C6 cells. Levels of cleaved caspase-7 and poly (ADP-ribose) polymerase (PARP), but not cleaved caspase-3, were also increased after cordycepin treatment. Specific inhibitors for p53 and caspases abrogated cordycepin-induced caspase-7 and PARP cleavage, and prevented cordycepin-induced apoptosis. Moreover, siRNA knockdown of p53 blocked cordycepin-induced cleavage of caspase-7 and PARP. Both adenosine 2A receptor (A2AR) antagonist and small interference RNA (siRNA) knockdown of A2AR blocked cordycepin-induced apoptosis, p53 activation, and caspase-7 and PARP cleavage. These may provide a new strategy of cordycepin for glioma therapy in the future. © 2014 Elsevier Ireland Ltd. All rights reserved.
Chen K.-T.,China Medical University at Taichung |
Wu C.-H.,Taipei Medical University |
Tsai M.-H.,China Medical University at Taichung |
Wu Y.-C.,Ching Kuo Institute of Management and Health |
And 3 more authors.
Behavioural Brain Research | Year: 2017
Sarcosine, an N-methyl-D-aspartate receptor enhancer, can improve depression-like behavior in rodent models and depression in humans. We found that a single dose of sarcosine exerted antidepressant-like effects with rapid concomitant increases in the mammalian target of rapamycin (mTOR) signaling pathway activation and enhancement of α-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor (AMPAR) membrane insertion. Sarcosine may play a crucial role in developing novel therapy for depression. For a detailed understanding of sarcosine, this study examined the effects of long-term sarcosine treatment on the forced swim test (FST), mTOR signaling, and AMPAR membrane insertion in rats. The effects of long-term sarcosine treatment were examined in naive rats and rats exposed to chronic unpredictable stress (CUS). Long-term sarcosine treatment (560 mg/kg/d for 21 d) significantly ameliorated the increased immobility induced by CUS in the FST, reaffirming the potential role of sarcosine as an antidepressant for depressed patients. The same long-term treatment exhibited no such effect in naive rats despite increased mTOR activation and AMPAR membrane insertion in both groups. Our findings clearly show CUS-exposed rats are sensitive to long-term sarcosine treatment in FST and the response at the same dose is absent in naïve rats. Nevertheless, the distinct sensitivity to long-term sarcosine treatment in rats with or without CUS is not associated with the activated mTOR signaling pathway or increased AMPAR membrane insertion. Additionally, understanding the behavioral and molecular basis of distinct responses is vital important for developing personalized treatment programs to increase the probability of success when treating depression. © 2016
Chen Y.-C.,Ching Kuo Institute of Management and Health
MATEC Web of Conferences | Year: 2017
This study explores the KSFs of TTQS, as introduced in international hotels in Taiwan. The method to carry out this study was using a survey AHP method. The findings of this study are, as follows: (1) Order of importance of the four dimensions: organizational management, training design, training execution, and result assessment, shows that when introducing TTQS, tourist hotels must first focus on reforming the organizational system. They should change the previous model to authorize training of the operational department. The training department should not play a passive role, and must establish a systematic training model with documents to dominate, as well as assist and audit training operations. (2) Among the 19 key factors of the sub-dimensions, the top 5 are high-ranking supervisors' high degree of support and participation, TTQS knowledge of persons in charge, connection between target members' and organizational needs, specific training goals before training, and regular training organization experience before introduction of TTQS. Based on the order, "high-ranking supervisors' support and participation" is the most important factor for organizations to introduce the TTQS system. © The Authors, published by EDP Sciences, 2017.
Huang C.-C.,China Medical University at Taichung |
Lai C.-J.,China Medical University at Taichung |
Tsai M.-H.,China Medical University at Taichung |
Wu Y.-C.,Ching Kuo Institute of Management and Health |
And 5 more authors.
BMC Neuroscience | Year: 2015
Background: It is well documented that the nitric oxide (NO) might be directly involved in brain response to hypobaric hypoxia, and could contribute to memory deficiencies. Recent studies have shown that melatonin could attenuate hypoxia or ischemia-induced nerve injuries by decreasing the production of free radicals. The present study, using immunohistochemical and immunoblot methods, aimed to explore whether melatonin treatment may affect the expression of nitric oxide system and protein nitration, and provide neuroprotection in the rat hippocampus injured by hypobaric hypoxia. Prior to hypoxic treatment, adult rats were pretreated with melatonin (100 mg/kg, i.p.) before they were exposed to the altitude chamber with 48 Torr of the partial oxygen concentration (pO2) for 7 h to mimic the ambience of being at 9000 m in height. They were then sacrificed after 0 h, 1, and 3 days of reoxygenation. Results: The results obtained from the immunohistochemical and immunoblotting analyses showed that the expressions of neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), nitrotyrosine (Ntyr) and Caspase 3 in the hypoxic hippocampus were increased from 0 h to 3 days of reoxygenation. Interestingly, the hypoxia-induced increase of nNOS, eNOS, iNOS, Ntyr and Caspase 3 protein expression was significantly depressed in the hypoxic rats treated with melatonin. Conclusions: Activation of the nitric oxide system and protein nitration constitutes a hippocampal response to hypobaric hypoxia and administration of melatonin could provide new therapeutic avenues to prevent and/or treat the symptoms produced by hypobaric hypoxia. © 2015 Huang et al.
Wang J.-J.,National Cheng Kung University |
Lin Y.-H.,Ching Kuo Institute of Management and Health |
Hsieh L.-Y.,Chia Yi Christian Hospital
Aging and Mental Health | Year: 2011
Objective: The purpose of this research was to test the clinical use of the gerotranscendence (GT) theory and its influence on GT perspective, depression, and life satisfaction among a group of institutionalized elders. Methods: An experimental study utilizing pre-post group design and random assignment of elders into intervention and control group was conducted. The intervention support group was implemented in the experimental group once a week for eight weeks for 60 min. Over the eight-week period, 35 subjects in the experimental group and 41 in the control group completed the study. Data were collected one week before and one week after the intervention for both groups. Instruments include the GT Scale, Geriatric Depression Scale short form, and Life Satisfaction scale. Data were analyzed by paired t-test, Wilcoxon, McNemar, and analysis of co-variance. Results: Pre- and post-test scores on the GT perspective and life satisfaction were significantly increased (p = 0.000) in the experimental group. The mean depression score of the experimental group showed a slight but not significant decrease (p = 0.06). However, significant post-test differences were noted between groups in GT perspective, depression, and life satisfaction (p = 0.000, 0.01, and 0.000). Conclusion: The findings of this study suggest that after participating in a GT support group, institutionalized elders' GT perspective and life satisfaction were enhanced, and depression reduced. The positive effects demonstrated by this study can be extended and applied to the clinical health promotion of institutionalized elders. Ongoing GT intervention is encouraged to promote mental and spiritual health among institutionalized elders. © 2011 Taylor & Francis.
Lai J.C.-Y.,Ching Kuo Institute of Management and Health
Menopause | Year: 2016
OBJECTIVE:: The study compared the in-hospital complications and related outcomes between women who underwent bilateral salpingo-oophorectomy at hysterectomy for benign diseases and those who had hysterectomy only. METHODS:: We conducted a population-based, retrospective cohort study using data from Taiwanʼs National Health Insurance program. Women who underwent concurrent bilateral salpingo-oophorectomy at hysterectomy for benign indications (n?=?34,509) were compared with those who had hysterectomy only (n?=?176,305). Separate models were estimated to account for the effect of baseline comorbid condition, age, and hysterectomy approach on the relationship between bilateral salpingo-oophorectomy and study outcomes. A secondary analysis was also performed to evaluate the association of inpatient readmission within 30 days and complications among women who underwent bilateral salpingo-oophorectomy. RESULTS:: The addition of a bilateral salpingo-oophorectomy to hysterectomy was associated with a lower risk of surgical complications, a longer length of hospital stay, and an increased risk of inpatient readmission within 30 days. Among women who underwent bilateral salpingo-oophorectomy, women with complications were also more likely to require inpatient readmission within 30 days than those without complications. Our data also suggested that bilateral salpingo-oophorectomy was not associated with an overall risk of medical complications, with the exception of urethral obstruction. The relationships remained even after adjustments by age, surgical indications, hysterectomy approach, and health-related risk factors, such as baseline comorbid condition and status of any prior catastrophic illness. CONCLUSIONS:: Bilateral salpingo-oophorectomy at hysterectomy for benign conditions is not associated with an increased risk of in-hospital complications. © 2016 by The North American Menopause Society.
Hsu P.-S.,Ching Kuo Institute of Management and Health
Applied Mechanics and Materials | Year: 2013
The purpose of this study is aims to develop an inventory assessing self-determined motivation for e-Learning courses in college. The study involves 397 college students, with exploratory factor analysis and Cronbach alpha scale reliability, and the validity of the test. Selfdetermined learning motivation scale is accordance with the Learning Self-Regulation Questionnaire (SRQ-L) by Williams & Deci (1996) and Black & Deci (2000). It compiled and revised the initial inventory consisted of 14 items, it divided into four dimensions, such as, "Intrinsic motivation", " Identified regulation", "Introjected Regulation ", and "External regulation", the total explained variance was 71.35%. The Cronbach alpha is.87, with acceptable construct validity and reliability of measurement tools, it can provide the future development of e-Learning courses. © (2013) Trans Tech Publications, Switzerland.
Hsiao W.-L.,National Taiwan University of Science and Technology |
Ho W.-L.,Ching Kuo Institute of Management and Health |
Chou C.-C.,National Taiwan University of Science and Technology
International Journal of Food Microbiology | Year: 2010
Cronobacter spp., formerly Enterobacter sakazakii, are considered emerging opportunistic pathogens and the etiological agent of life-threatening bacterial infections in infants. In the present study, C. sakazakii BCRC 13988 was first subjected to sub-lethal heat treatment at 47 °C for 15 min. Survival rates of the heat-shocked and non-shocked C. sakazakii cells in phosphate buffer solution (PBS, pH 4.0) containing organic acids (e.g. acetic, propionic, citric, lactic or tartaric acid), simulated gastric juice (pH 2.0-4.0), and bile solution (0.5 and 2.0%) were examined. Results revealed that sub-lethal heat treatment enhanced the test organism's tolerance to organic acids, although the extent of increased acid tolerance varied with the organic acid examined. Compared with the control cells, heat-shocked C. sakazakii cells after 120-min of exposure, exhibited the largest increase in tolerance in the lactic acid-containing PBS. Furthermore, although heat shock did not affect the behavior of C. sakazakii in bile solution, it increased the test organism's survival when exposed to simulated gastric juice with a pH of 3.0-4.0. © 2010.
Fong J.J.,University of California at Berkeley |
Chen T.-H.,Ching Kuo Institute of Management and Health
Conservation Genetics | Year: 2010
Field surveys in Taiwan have uncovered turtles presumed to be hybrids based on their intermediate morphology. We sequenced a mitochondrial (ND4) and nuclear (R35) gene of two putative hybrid individuals, along with representatives of the potential parental species (Mauremys mutica, M. reevesii, M. sinensis), to determine their genetic identity. Based on our data, both individuals are hybrids, with independent, recent origins resulting from the mating of a female M. reevesii and a male M. sinensis. Since we question whether the highly traded M. reevesii is endemic to Taiwan, this hybridization could represent human-mediated genetic pollution. We also discuss the implications of our findings on turtle conservation in Taiwan. © 2010 The Author(s).
Tsai C.-J.,National Taiwan Ocean University |
Tsai C.-J.,Ching Kuo Institute of Management and Health |
Sun Pan B.,National Taiwan Ocean University
Journal of Agricultural and Food Chemistry | Year: 2012
The fatty acid compositions of 21 species of marine macroalgae, including 5 species of Chlorophyta (green algae), 13 of Rhodophyta (red algae), and 3 of Heterokontophyta (brown algae), were collected from northeastern Taiwan to survey their functional lipids. The lipid contents of green algae ranged from 15.36 to 20.15 mg/g, dry basis (db), and were characterized by a high content of C18:2 and C18:3, red algae (18.57-28.34 mg/g db) were high in C20:4 and C20:5, and brown algae (13.11-19.56 mg/g db) were high in C18:4, C20:4, and C:20:5. All algal lipids contained fatty acids of odd-number carbons, C17:0, and C17:1. Red algae had relatively higher levels of polyunsaturated fatty acids (PUFAs) and were richer in eicosapentaenoic acid (EPA) than green and brown algae. A red alga, Porphyra crispata, was extracted with ethanol and separated on a hydrophobic column (Diaion HP-20 column) to obtain sulfoglycolipids (sulfoquinovosyldiacylglycerols, SQDGs). The main fatty acids in SQDGs were palmitic acid (C16:0), 33.3%; EPA (C20:5), 30.0%; arachidonic acid (C20:4), 12.7%; oleic acid (C18:1), 7.52%; and stearic acid (C18:0), 6.83%. The n-3/n-6 ratio was 1.9, whereas the authentic standard, spinach SQDG, did not contain n-3 fatty acids. Sulfoglycolipids inhibited the growth of human hepatocellular carcinoma cell line (HepG2). The IC50 was 126 μg/mL, which is lower than that of the spinach SQDG (255 μg/mL). © 2012 American Chemical Society.