Li Z.,Key Laboratory for Regenerative Medicine |
Li Z.,Chinese University |
Li Z.,Jinan University |
Li Z.,Chinese University of Hong Kong |
And 18 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011
BACKGROUND: Adult bone marrow-derived stem cells are promising cell source in cell therapy. Tracking of adult bone marrow-derived stem cells is crucial to demonstrate the mechanism of stem cell migration and differentiation, and develop novel strategy for functional regeneration and stem cell therapy. OBJECTIVE: To explore effects of transfected adult bone marrow clonogenic stem cells (AMCSCs) on cell phenotype, proliferation and cardiac differentiation potential. METHODS: Plasmid-encoded reporter gene maxGFP was used for nucleofection of AMCSCs with U-23 program. Growth curves of AMCSCs before and after nucleofection were compared based on results of MTT assay. AMCSCs before and after nucleofection were treated with 3 μmol/L 5-azacytidine for inducing cardiac differentiation. The cardiac differentiation specific markers, GATA4 and MLC-2v, were applied to confirm cardiac differentiation by RT-PCR. The maxGFP transfected AMCSCs were conducted the intramyocardium injection into an adult Sprague-Dawley rat left anterior descending coronary artery (LAD) ligation model to trace the in vivo expression of transfected maxGFP gene. Fluorescence images of the injected heart were analyzed on days 2 and 7 postinjection. RESULTS AND CONCLUSION: At 24 hours following nucleofection, the transfection efficiencies of AMCSCs at passages 47 and 119 were 49.4% and 43.1%. On 5 hours, green fluorescence positive cells were observed. Following nucleofection, AMCSCs maintained round shape, and could adhere and show clone-shape growth. MTT assay results demonstrated that the passages 47 and 119 of AMCSCs exhibited similar growth curves before and after nucleofection. Mean population doubling time was 8.57 and 10.28 hours in passages 47 and 119 of AMCSCs prior to nucleofection, and 9.42 and 10.42 hours following nucleofection (P =0.551, P=0.774). RT-PCR results showed that AMCSCs expressed GATA4 before and after 5-azacitidine treatment prior to nucleofection, and strongly expressed MLC-2v strip after treatment. AMCSCs expressed GATA4 prior to and following 5-azacitidine treatment after nucleofection, and strongly expressed MLC-2v after treatment. No significant difference was determined in above-mentioned indexes prior to and following nucleofection. In vivo experiment results demonstrated that a few green fluorescence positive cells were apparent in injected myocardium on days 2 and 7 following transfected AMCSCs injection. Results indicated that nucleofection is an effective and fast method for transfection of exogenous DNA into cell. The AMCSCs which are experienced with nucleofection are able to maintain their morphology, proliferation and cardiac differentiation potential. However, only a few transfected AMCSCs express the transferred gene, GFP, after intramyocardium injection.
Park J.H.,Future Technology Research Association International FTRA |
Hung J.C.,Chinese University |
Yen N.Y.,University of Aizu |
Jeong Y.-S.,Dongguk University
Journal of Internet Technology | Year: 2014
The special issue 2014, Volume 15 of Journal of Internet Technology presents an overview of the state-of-the-art of issues and solution guidelines for the Advanced Convergence Technologies; Big Data, IoT and Cloud Computing. Joon-Min Gil and co-researchers presented a user-created computing framework for desktop grids as entitled 'Organizing a User-Created Computing Environment by RESTful Web Service Open APIs in Desktop Grids.' Using the framework, application developers can utilize DGSs easily and conveniently as computational tools in order to solve their own applications. Sadiq Almuairfi and co-researchers compared IPAS with other authentication schemes by performing two experiments and asking participants to answer a questionnaire as entitled 'A Comparative Study of Authentication Schemes with Security and Usability of IPAS.' They explained the usability and security of IPAS from the users' point of view. Prosper Mafole et al. proposed a novel fragmentation scheme called backoff-free fragment retransmission (BFFR).
News Article | March 14, 2016
Academics will carry out research in China to resolve copyright issues posed by the new technology, which allows the copying of physical objects by scanning them. They hope to develop a single system for protecting intellectual property which could be used around the world. This automated licensing platform framework will enable 3D printing companies to licence 3D printed content and files in new ways. This could be an online database, or digital versions of a watermark to prevent unauthorised copying. Academics will analyse what the impact of this system would be on copyright law, in particular copyright law in China and internationally. The use of 3D printing is rapidly growing, but innovation has become constrained because of the lack of clarity over legal rules. The research project, which will run until December 2016, will be carried out in partnership with academics from the University of Nottingham Ningbo China, Durham University's Law School, the University of Sussex, and the Chinese University of Political Science and Law. The study, funded by the Arts and Humanities Research Council and many of the universities involved, will see academics collaborate with colleagues from around the world to establish a viable, technical and operational online licensing system and a workable legal framework for IP licensing. The academics, who are specialists in IP law, international law and contract law, will carry out interviews with 3D printing companies to discover how they operate and their needs, and spend time with one firm in particular to write a case study. Dr James Griffin, from the Law School, who will lead the project, said: "We are in contact with 3D printing companies who wish to develop a new means of creating and disseminating 3D printed content utilising their 3D printing systems and to capture new business opportunities. "However, to date they have been limited in their opportunities to do so because of the complex legal licensing environment and the lack of appropriate digital licensing standards. We can enable these companies to learn how to exploit their products." In the UK, the relevant regulatory body called the Copyright Hub has set out a list of licensing standards to use in online licensing systems. Academics will explore the appropriateness of these guidelines in the development of the proposed licensing system in China. The proposed licensing system will help companies develop their markets because they will be able to licence works in new ways. The system will also help the UK regulatory Copyright Hub find out how their system operates in practice both in the UK and in China. Explore further: 3D printing to transform the economy, UK report claims
News Article | October 31, 2016
SHENZHEN, China, Oct. 31, 2016 /PRNewswire/ -- The first World Medical Robotics Conference, hosted by Medical Robotics Society (MRS) and organized by ROBO Health Institute, was held in Shenzhen on Oct 29th -30th, 2016. The event was chaired by Yangsheng Xu, dean of Chinese University...
News Article | March 15, 2016
A shoddy building collapses in an earthquake, people are injured, then hospitals and health professionals respond. So Ardalan has worked to strengthen Iran's healthcare system - from hospitals to the country's 150,000 female community health volunteers - by training them in what to do when disasters strike. "Disasters have an impact on public health, and health systems have to take a proactive approach, preventive measures to reduce the risk of disasters," said Ardalan, chair of the Disaster and Emergency Health Academy at Tehran University of Medical Sciences. Ardalan was one of several speakers at a conference held last week in Bangkok to discuss implementation of health aspects of the Sendai Framework for Disaster Risk Reduction adopted by U.N. member states a year ago. Health is a relatively new aspect of disaster risk reduction. The Sendai accord was the first to give health a higher profile, with measures to protect health by reducing damage to hospitals and ensuring medical care continues in disasters. It also tackles the risks of epidemics and pandemics. In the decade ending in 2014, disasters caused $1.4 trillion in damage, killed about 700,000 people and affected 1.7 billion others, according to the U.N. Office for Disaster Risk Reduction. Key infrastructure and healthcare facilities are often wiped out. The 2008 Sichuan earthquake in China damaged or destroyed 11,000 hospitals, while the 2004 Indian Ocean tsunami damaged 61 percent of health facilities in Aceh, Indonesia, killed 7 percent of the area's health workers and 30 percent of its midwives, according to the Overseas Development Institute. Disaster health experts like Ardalan have focused on building resilience and preparing for such catastrophes. Iran has worked to ensure its hospitals have disaster contingency plans, including evacuation plans in the event of an earthquake or flood, said Ardalan, who is also a visiting scientist at the Harvard School of Public Health and an adviser to the World Health Organization. Iran has also trained its community health volunteers - all women - to conduct household training, which includes drawing a household earthquake risk map to show danger spots near big windows or under large ceiling lights, as well as safe spots under tables or near pillars. Last year, the volunteers trained 500,000 households across Iran, he said. "We believe it's better to be proactive, work with them, so they are sensitive to their safety and know how to react if something happens," he said on the sidelines of the conference. "It's a very-cost effective intervention for the entire society." Similar efforts are under way to provide health and disaster preparedness for ethnic minority communities in rural China. Emily Ying Yang Chan, who worked for Médecins Sans Frontières for 17 years and now heads the disaster and medical humanitarian response center at Chinese University in Hong Kong (CUHK), began the ethnic minority health program about six months after the Sichuan quake. The typical community her team works with is two flights and a seven-hour bumpy car ride away, though one village, 5,000 meters above sea level on the Tibetan Plateau, took 17 hours to get to in a four-wheel-drive car. Chan's approach has been to provide the knowledge or help that villagers request, on condition that her team gets to conduct disaster risk training. Most communities want to learn more about economic development, though women also ask them to teach their husbands not to smoke or how to read food labels. In return, Chan and her students from the Collaborating Centre for Oxford University and CUHK for Disaster and Medical Humanitarian Response (CCOUC) give health advice such as not burning waste indoors and reducing salt intake. Then the team helps the community to prepare disaster kits, handing out red cloth bags, with large Chinese characters that read "rescue bag". They put in soap, a towel, a bottle of water and non-perishable food, as well as a manual battery-less torch and a multipurpose knife with a can opener - which Chan said has often been missing from aid packages. "A lot of agencies sent food supplies, but forgot to send a can opener, and many people come to the clinic with cuts because they use whatever they can to cut (open the can)," she said. The ethnic minority health program team has worked in 11 villages, visiting each one four times over a two-year period. The biggest challenge now, Chan says, is digesting the data they have gathered, to improve and scale up assistance for the villagers.