Zhejiang Province Hospital of Traditional Chinese Medicine

Hangzhou, China

Zhejiang Province Hospital of Traditional Chinese Medicine

Hangzhou, China
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Ma Z.-X.,China Pharmaceutical University | Xu Y.,China Pharmaceutical University | Zhao H.,Zhejiang Province Hospital of Traditional Chinese Medicine | Sun F.-M.,China Pharmaceutical University | And 3 more authors.
Yaoxue Xuebao | Year: 2017

Transglutaminase (TG) posttranslational modification of antibody permits more precisely conjugating. Based on the amino acid sequence of an anti-CD24 antibody (cG7), this article is aimed to generate a deglycosylated cG7 mutant (cG7Q). Firstly, we introduced additional glutamines at position 297 (N297Q) by site-directed mutagenesis, and then transfected the recombinant plasmids into CHO-s cells via electroporation method and screened by Dot blot assay. Subsequently, cG7Q was expressed and purified through Protein A affinity chromatography, further identified by SDS-PAGE electrophoresis and Western blot. Its affinity was detected with surface plasmon resonance and flow cytometry assay, and ADCC effect was determined by lactate dehydrogenase (LDH) release. Eventually, a cG7 mutant, cG7Q was successfully expressed with sequence-specific conjugation sites for further study.


Yu P.,Zhejiang Cancer Hospital | Cheng X.,Zhejiang Province Hospital of Traditional Chinese Medicine | Du Y.,Zhejiang Cancer Hospital | Yang L.,Zhejiang Cancer Hospital | And 6 more authors.
Journal of Gastrointestinal Surgery | Year: 2015

Aim: The aim of this study was to determine the clinicopathological features, surgical management, and prognosis of solid pseudopapillary neoplasms (SPNs) of the pancreas. Methods: This study conducted a retrospective analysis of 97 patients who underwent surgery for a pathologically confirmed SPN in five hospitals between January 1996 and December 2014. Results: The 97 cases included 93 female and 4 male patients, and the average age was 31.2 years. The tumor was located in the body or tail (70.1 %), the head (20.6 %), and the neck (9.3 %). All patients underwent surgical exploration, including distal pancreatectomy (63.9 %), pancreaticoduodenectomy (20.6 %) (partial portal vein or superior mesenteric vein resection and artificial vascular graft reconstruction performed in 4.1 % of the patients), central pancreatectomy (10.3 %), enucleation (5.2 %), and liver resection (1.0 %). 16.5 % of the patients had malignant tumors. The positive rate of Ki-67 was 66.7 % in patients diagnosed with a malignant neoplasm and was comparable to 8.4 % of the patients diagnosed to have a benign neoplasm (p < 0.001). After a median follow-up of 70.1 months, three patients had recurrence and one patient died of liver metastasis. Conclusions: SPN is a rare neoplasm with low malignant potential. Surgical resection is warranted even in the presence of local invasion or metastases as patients demonstrate excellent long-term survival. Positive immunoreactivity for Ki-67 may predict the malignant potential and poor outcome of SPNs. © 2015, The Society for Surgery of the Alimentary Tract.

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