Chinese PLA Medical School

Beijing, China

Chinese PLA Medical School

Beijing, China

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Li B.-S.,Chinese PLA Medical School | Sun D.-X.,Chinese PLA Medical School | Sun D.-X.,Bethune International Peace Hospital of Chinese PLA
World Chinese Journal of Digestology | Year: 2016

During hepatitis B virus (HBV) infection, covalently closed circular DNA (cccDNA) acts as the template for the synthesis of viral RNA and new virions. Current therapies rarely achieve an elimination of cccDNA. Biosynthesis of relaxed circular (RC) DNA by reverse transcription of the viral pregenomic RNA is now understood quite well, yet conversion of RC-DNA to cccDNA is still obscure. Conceptual and recent experimental data link cccDNA formation to cellular DNA repair, which is increasingly appreciated as a critical interface between cells and viruses. This review aims to summarize current knowledge on cccDNA molecular biology, to highlight the experimental restrictions that have hitherto hampered faster progress and to discuss cccDNA as a target for potentially curative therapies for chronic hepatitis B. © 2016 Baishideng Publishing Group Inc. All rights reserved.


Ji Y.,Chinese PLA Medical School | Ji Y.,302 hospital of PLA | Li B.,302 hospital of PLA | Zhu Z.,302 hospital of PLA | And 4 more authors.
Biomedicine and Pharmacotherapy | Year: 2015

Background: Wiskott-Aldrich syndrome verprolin-homologous (WAVE) 3 has been reported to be implicated in various malignant tumors, but its role in hepatocellular carcinoma (HCC) remains elusive. The aim of this study was to investigate the effect of WAVE3 on the behaviors of HCC cells and to evaluate its clinical impact. Materials and methods: A total of 120 paired of HCC and adjacent non-cancerous tissues were used to detect expression pattern of WAVE3 by immunohistochemistry. Then, the associations of WAVE3 expression with clinicopathologic characteristics and patients' prognosis were examined. The roles of WAVE3 in migration and invasion of HCC cell line HepG2 were also evaluated in vitro. Results: Positive immunostaining of WAVE3 protein was predominantly observed in the cytoplasm of HCC cells. Compared to adjacent non-cancerous tissues, the expression levels of WAVE3 protein were significantly upregulated in HCC tissues (P< 0.001). Additionally, high WAVE3 expression was significantly associated with advanced tumor stage (P= 0.008) and positive distant metastasis (P= 0.001). Then, high WAVE3 expression correlated significantly with poor prognosis, and WAVE3 status was identified as an independent significant prognostic factor. Moreover, small interfering RNA targeting WAVE3 was used to inhibit the expression of WAVE3 in HepG2 cells. We found that suppression of WAVE3 could inhibit migration and invasion of HepG2 cells. Conclusion: Our clinical study have characterized WAVE3 as biomarker for HCC progression and metastasis, and more importantly, have identified it as an independent prognostic marker for HCC patients. Our data also indicated that WAVE3 is pivotal in controlling oncogenic phenotypes of human HCC cells. © 2014 Elsevier Masson SAS.


Liu T.,Chinese PLA General Hospital | Zhang B.,Chinese PLA Medical School | Jin X.,Chinese PLA General Hospital | Wang W.,Chinese PLA General Hospital | And 4 more authors.
Eye (Basingstoke) | Year: 2014

PurposeTo describe the characteristic ophthalmic phenotypes of a large Chinese family with familial amyloid polyneuropathy due to a missense mutation in transthyretin (TTR) (c.307 C>G).MethodsTwenty-seven individuals (12 affected, 15 unaffected) from a five-generation Chinese family underwent general medical examination and comprehensive ophthalmic examination, including best correct visual acuity, intraocular pressure measurements, Schirmer test, slitlamp examination, fundoscopy, and ocular ultrasonography. Histological examination of vitreous biopsies using Congo red staining and immunohistochemistry was performed. Cardiovascular magnetic resonance (CMR), electrocardiogram, and echocardiogram were used to evaluate cardiac amyloidosis. Electromyography was used to evaluate nerve function. All four exons of TTR were amplified by PCR, sequenced using a Bigdye terminator v3.1 cycle sequencing kit and analyzed on an ABI 3700XL Genetic Analyzer.ResultsAll 12 affected individuals in the family had ocular manifestations, including severe vitreous opacities, secondary glaucoma, xerophthalmia, dyscoria, and attenuated retinal arteries. Congo red staining demonstrated amyloid deposits in the vitreous, and immunohistochemical staining confirmed the deposition of TTR proteins in the vitreous. Twelve individuals had polyneuropathy, and electromyography detected functional damage in peripheral nerves. One individual was diagnosed with cardiac amyloidosis by CMR. Direct sequencing revealed the heterozygous missense mutation in TTR (c.307 C>G p.Gly83Arg) in all 12 affected individuals. The mutation co-segregated with the disease phenotype and was absent in 100 normal controls.ConclusionsVitreous opacity is very common in patients with the TTR Gly83Arg mutation; other clinical characteristics associated with the mutation include polyneuropathy and cardiac amyloidosis. © 2014 Macmillan Publishers Limited All rights reserved.


Chen Y.,Chinese PLA Medical School | Zhang Q.,Chinese PLA Medical School | Zhang H.,Chinese PLA General Hospital
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2015

OBJECTIVE: To compare cricothyroid membrane puncture directed tracheostomy (CMPDT) with conventional surgical tracheostomy (ST) and cricothyroidotomy (CT) and to evaluate the feasibility, efficacy and safety of this novel technique.METHODS: 15 minipigs were divided randomly into 3 groups, 5 in each. After general anesthesia, CMPDT, ST and CT were performed respectively according to the established techniques when SPO₂reached 80% (T0). Procedure duration, ECG and arterial blood gas results were recorded. Complications were recorded and scored according to an established score scale by an observer blinded to the grouping.RESULTS: Airway was successfully established in all animals (15/15). ECG monitor showed T-wave decreased and Q-T shortened after seasing of oxygen supply and both recovered rapidly to normal levels after reoxygenation. There were no significant differences between 3 groups in HR, BP, SPO2, SaO₂, PaO₂, PaCO₂and pH at pre-apnea, T0 or post-operation, but with significant intragroup variation in the parameters before and after operation. The time for CMPDT, ST and CT was (174 ± 34) s, (619 ± 128) s and (86 ± 12) s respectively. Three of 5 minipigs in ST group experienced hypotension due to longer time of hypoxia. 1 and one had minor bleeding and stoma infection after surgery. One of 5 animals in CT group had minor laryngeal cartilage injury leading to difficult decannulation, postoperative fiber bronchoscopy showed no subglottic stenosis. The complication scores were 13, 9, and 3 for ST, CT and CMPDT, respectively.CONCLUSIONS: All 3 methods can provide with effective airway access with no significant differences in ventilation effect, however CMPDT has short recovery time for SpO2 and other vital signs with the lowest complication score. The animal experiment suggests that CMPDT is a fast, safe and effective surgical technique for emergency airway.


PubMed | Chinese PLA General Hospital and Chinese PLA Medical School
Type: Journal Article | Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2015

To compare cricothyroid membrane puncture directed tracheostomy (CMPDT) with conventional surgical tracheostomy (ST) and cricothyroidotomy (CT) and to evaluate the feasibility, efficacy and safety of this novel technique.15 minipigs were divided randomly into 3 groups, 5 in each. After general anesthesia, CMPDT, ST and CT were performed respectively according to the established techniques when SPOreached 80% (T0). Procedure duration, ECG and arterial blood gas results were recorded. Complications were recorded and scored according to an established score scale by an observer blinded to the grouping.Airway was successfully established in all animals (15/15). ECG monitor showed T-wave decreased and Q-T shortened after seasing of oxygen supply and both recovered rapidly to normal levels after reoxygenation. There were no significant differences between 3 groups in HR, BP, SPO2, SaO, PaO, PaCOand pH at pre-apnea, T0 or post-operation, but with significant intragroup variation in the parameters before and after operation. The time for CMPDT, ST and CT was (174 34) s, (619 128) s and (86 12) s respectively. Three of 5 minipigs in ST group experienced hypotension due to longer time of hypoxia. 1 and one had minor bleeding and stoma infection after surgery. One of 5 animals in CT group had minor laryngeal cartilage injury leading to difficult decannulation, postoperative fiber bronchoscopy showed no subglottic stenosis. The complication scores were 13, 9, and 3 for ST, CT and CMPDT, respectively.All 3 methods can provide with effective airway access with no significant differences in ventilation effect, however CMPDT has short recovery time for SpO2 and other vital signs with the lowest complication score. The animal experiment suggests that CMPDT is a fast, safe and effective surgical technique for emergency airway.


PubMed | University of California at San Diego, Chinese PLA General Hospital, Chinese PLA Medical School and Nankai University
Type: Journal Article | Journal: Eye (London, England) | Year: 2014

To describe the characteristic ophthalmic phenotypes of a large Chinese family with familial amyloid polyneuropathy due to a missense mutation in transthyretin (TTR) (c.307 C>G).Twenty-seven individuals (12 affected, 15 unaffected) from a five-generation Chinese family underwent general medical examination and comprehensive ophthalmic examination, including best correct visual acuity, intraocular pressure measurements, Schirmer test, slitlamp examination, fundoscopy, and ocular ultrasonography. Histological examination of vitreous biopsies using Congo red staining and immunohistochemistry was performed. Cardiovascular magnetic resonance (CMR), electrocardiogram, and echocardiogram were used to evaluate cardiac amyloidosis. Electromyography was used to evaluate nerve function. All four exons of TTR were amplified by PCR, sequenced using a Bigdye terminator v3.1 cycle sequencing kit and analyzed on an ABI 3700XL Genetic Analyzer.All 12 affected individuals in the family had ocular manifestations, including severe vitreous opacities, secondary glaucoma, xerophthalmia, dyscoria, and attenuated retinal arteries. Congo red staining demonstrated amyloid deposits in the vitreous, and immunohistochemical staining confirmed the deposition of TTR proteins in the vitreous. Twelve individuals had polyneuropathy, and electromyography detected functional damage in peripheral nerves. One individual was diagnosed with cardiac amyloidosis by CMR. Direct sequencing revealed the heterozygous missense mutation in TTR (c.307 C>G p.Gly83Arg) in all 12 affected individuals. The mutation co-segregated with the disease phenotype and was absent in 100 normal controls.Vitreous opacity is very common in patients with the TTR Gly83Arg mutation; other clinical characteristics associated with the mutation include polyneuropathy and cardiac amyloidosis.


PubMed | Chinese PLA General Hospital and Chinese PLA Medical School
Type: Journal Article | Journal: PloS one | Year: 2016

Surgery carries the best hope for cure in the treatment of cholangiocarcinoma (CC), whereas surgical outcome is not fully satisfactory. Bio-molecular markers have been used to improve tumor staging and prognosis prediction. Mucin antigen MUC4 (MUC4) has been implicated as a marker for poor survival in various tumors. However, prognostic significance of MUC4 for patients with CC remains undefined. The aim of the present meta-analysis was to investigate the association between MUC4 expression and overall survival (OS) of patients with resected CC.The meta-analysis was conducted in adherence to the MOOSE guidelines. PubMed, Embase databases, Cochrane Library and the Chinese SinoMed were systematically searched to identify eligible studies from the initiation of the databases to April, 2016. OSs were pooled by using hazard ratio (HR) with corresponding 95% confidence interval (CI). Random effect models were utilized because of the between-study heterogeneities.Five studies reporting on 249 patients were analyzed: 94 (37.75%) were in positive or high expression group and 155 (62.25%) in negative or low expression group. The pooled HR for positive or high expression group was found to be 3.04 (95% CI 2.25-4.12) when compared with negative or low expression group with slight between-study heterogeneities (I2 3.10%, P = 0.39). The result indicated that a positive or high expression level of MUC4 was significantly related to poor survival in patients with resected CC. A commensurate result was identified by sensitivity analysis. The main limitations of the present meta-analysis were the rather small size of the studies included and relatively narrow geographical distribution of population.The result of this meta-analysis indicated that a positive or high expression level of MUC4 was significantly related to poor survival in patients with resected CC.


Li Y.,Chinese PLA Medical School | Wang Y.,Beijing Electrical Power Hospital | Ma L.,Chinese PLA Medical School
Genomics, Proteomics and Bioinformatics | Year: 2014

Although molecular mechanisms of heat stroke under physiological and pathological conditions have not yet been elucidated, a novel disease-associated gene encoding a calcium-binding protein, calsequestrin-1 (CASQ1), was suggested relevant based on results from a transgenic murine model. Here, we show the association between single nucleotide polymorphisms (SNPs) of CASQ1 and physiological parameters for heat stroke from a study involving 150 patients. Pooled DNA from heat stroke patients were subjected to sequencing and 3 SNPs were identified. Genotypes were assigned for all patients according to g. 175A>G, one SNP which leads to a nonsynonymous substitution (N59D) in the first exon of human CASQ1 gene. We analyzed the genotypic data with a linear model based on significance scores between SNP (175A>G) and heat stroke parameters. As a result, we found a significant association between SNP A175G and heat stroke (P<. 0.05). Further bioinformatics analysis of the 1-Mb flanking sequence revealed the presence of two genes that encode DDB1 and CUL4 associated factor 8 (DCAF8), and peroxisomal biogenesis factor 19 (PEX19), respectively, which might be functionally related to CASQ1. Our results showed that the blood calcium of patients with allele D increased significantly, compared to patients with allele N (P<. 0.05), which may result from the decreased calcium in muscle, suggesting that N59D in CASQ1 might account for the dysfunction of CASQ1 in calcium regulation during heat stroke. © 2014.


Deng S.,Chinese PLA Medical School | Hu N.,Chinese PLA Medical School
Chinese Journal of Rehabilitation Medicine | Year: 2015

Objective: To observe the effects of ankle stretch training on lower limb function in stroke patients and to explore its mechanism. Method: Twenty-five patients with stroke were randomly divided into two groups: 12 in ankle stretching group (experimental group) and 13 in straight leg raising group (control group). Both groups were trained by the leg rehabilitation robot(LR2) for 2 weeks, 12 sessions in total, 45 min/session. Before and after training, the spasticity of the ankle joint was assessed by isokinetic testing system.; Active peak torque (PT) of ankle joint muscle was also tested. Besides the active and passive range of motion (AROM, PROM) of the ankle, torqueangular velocity slope(SLOPE), modified Ashworth scale (MAS), clinical spasticity index (CSI) were tested also. Result: Before training, there was no significant difference between two groups in all the measured parameters. After 2 weeks training, the spasticity measured under different angular velocity showed a significant difference between the two groups except 240°/s. There was significant difference between the two groups in muscle strength at 60°/s, but not at 120°/s and 180°/s. Also, there was significant difference between the two groups in the SLOPE. The AROM, PROM, MAS value and CSI in experimental group were significantly better than control group(P<0.05). Conclusion: Ankle stretch training could effectively reduce spasticity in patients with stroke. Ankle joint mobility and its muscle strength might be improved also. © 2015, Editorial Board of Chinese Journal of Rehabilitation Medicine. All right reserved.


PubMed | The General Hospital of Beijing Military Command, The Third Peoples Hospital of Shizuishan City and Chinese PLA Medical School
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014

The aim of the current study was to observe the effects of suppressor of cytokine signaling 1 (SOCS1) silencing in human melanoma cells on cell biological behavior and interferon- (IFN-) sensitivity, and to investigate the use of SOCS1 as a therapeutic target in the treatment of melanoma. Western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to verify that SOCS1 interference effectively silenced the expression of SOCS1 in the Mel526 human melanoma cell line. For IFN- stimulation, western blot analysis was used to observe changes in expression levels of signal transduction and transcription activator (STAT) 1 and phosphorylated STAT (pSTAT) 1. Changes in the expression levels of IFN- regulatory factor 1 (IRF-1) were measured with RT-qPCR. Changes in the sensitivity of melanoma cells to IFN- were detected using an MTT assay. The cell proliferation rate was observed by cell counting and changes in the cell cycle were detected with flow cytometry. The results revealed that SOCS1 interference effectively silences SOCS1 expression in Mel526 cells. However, the S stage of the cell cycle was markedly extended. Following the inhibition of SOCS1 expression, the proliferation experiment demonstrated that the proliferation ability of Mel526 cells was decreased. Following IFN- stimulation, the expression levels of pSTAT and IRF-1 increased significantly compared with those in the controls. The MTT experiment showed that SOCS1 interference caused the median inhibitory concentration (IC50) of oxaliplatin in Mel526 cells to decrease significantly. In conclusion, SOCS1 interference reduced the proliferation ability of Mel526 human melanoma cells and increased their sensitivity to IFN-.

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