Chinese National Institute for Radiological Protection

Beijing, China

Chinese National Institute for Radiological Protection

Beijing, China
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Gao A.,Capital Medical University | Zuo X.,Capital Medical University | Liu Q.,Chinese National Institute for Radiological Protection | Lu X.,Chinese National Institute for Radiological Protection | And 2 more authors.
Toxicology Letters | Year: 2010

Benzene is an established hematotoxic carcinogen which can cause leukemia. DNA damage and disorder of repair capacity are the crucial mechanisms in leukemogenesis of benzene. DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine (5-aza) and histone deacetylase inhibitor, trichostatin A (TSA) are two kinds of key epigenetic modification reagents. The mRNA expression of poly(ADP-ribose) polymerases-1 (PARP-1), a pivotal repair gene, has been decreased by benzene. However, the effect of epigenetic modification on benzene-induced low PARP-1 expression has not been reported. In this study, lymphoblastoid cell line F32 was incubated by benzene and then further treated with 5-aza and TSA, alone or in combination. The reverse transcription-polymerase chain reaction and methylation-specific PCR were performed to examine the mRNA expression and methylation status of PARP-1, respectively. Results showed a dramatic decrease of PARP-1 mRNA expression and a simultaneously obvious increase in the level of PARP-1 methylation in benzene-treated cells compared to the control. Further, the PARP-1 mRNA expression was restored and the level of PARP-1 methylation was also reduced following epigenetic inhibitors, 5-aza and TSA, alone or in combination treatments. Taken together, methylation of PARP-1 promoter might be involved in the regulation of benzene-induced decrease of PARP-1 mRNA expression. © 2010 Elsevier Ireland Ltd.

Chen H.-P.,Liaoning University of Traditional Chinese Medicine | Yang K.,Beijing Normal University | Zheng L.-S.,Liaoning University of Traditional Chinese Medicine | You C.-X.,Beijing Normal University | And 2 more authors.
Pharmacognosy Magazine | Year: 2015

Context: It was found that the essential oil of Acorus calamus rhizomes showed insecticidal activity. Aim: The aim of this study was to determine the chemical composition of the essential oil from A. calamus rhizomes, evaluate insecticidal and repellant activity against Lasioderma serricorne (LS) and Tribolium castaneum (TC), and to isolate any insecticidal constituents from the essential oil. Materials and Methods: Essential oil from A. calamus was obtained by hydrodistillation and analyzed by gas chromatography (GC) flame ionization detector and GC-mass spectrometry. The insecticidal and repellant activity of the essential oil and isolated compounds was tested using a variety of methods. Results: The main components of the essential oil were identified to be isoshyobunone (15.56%), β-asarone (10.03%), bicyclo[6.1.0]non-1-ene (9.67%), shyobunone (9.60%) and methylisoeugenol (6.69%). Among them, the two active constituents were isolated and identified as shyobunone and isoshyobunone. The essential oil showed contact toxicity against LS and TC with LD 50 values of 14.40 and 32.55 μg/adult, respectively. The isolated compounds, shyobunone and isoshyobunone also exhibited strong contact toxicity against LS adults with LD 50 values of 20.24 and 24.19 μg/adult, respectively, while the LD 50 value of isoshyobunone was 61.90 μg/adult for TC adults. The essential oil, shyobunone and isoshyobunone were strongly repellent (98%, 90% and 94%, respectively, at 78.63 nL/cm 2 , after 2 h treatment) against TC. Conclusion: The essential oil, shyobunone and isoshyobunone possessed insecticidal and repellant activity against LS and TC.

Luo J.,Hexi University | Luo J.,Lanzhou University | Tuo F.,Chinese National Institute for Radiological Protection | Kong X.,Lanzhou University
Journal of Radioanalytical and Nuclear Chemistry | Year: 2011

The cross sections for formation of metastable state of 178Hf (178m2Hf, 574.215 keV, 31 y) and 179Hf ( 179m2Hf, 362.55 keV, 25.05 d) through reactions induced by 14.8 ± 0.2 MeV neutrons on natural hafnium were measured for the first time. The monoenergetic neutron beam was produced via the 3H(d, n) 4He reaction on ZF-300-II Intense Neutron Generator at Lanzhou University. Induced gamma activities were measured by a gamma-ray spectrometer with high-purity germanium (HPGe) detector. Measurements were corrected for gamma-ray attenuations, random coincidence (pile-up), dead time and fluctuation of neutron flux. The neutron fluence were determined by the cross section of 93Nb(n, 2n)92mNb reaction. The neutron energy in the measurement were by the cross section ratios of 90Zr(n, 2n) 89m+gZr and 93Nb(n, 2n)92mNb reactions. © 2011 Akadémiai Kiadó, Budapest, Hungary.

Zhao Y.,Beijing Normal University | Yan D.,Beijing Normal University | Zhang Q.,Beijing Normal University | Zhang Q.,Chinese National Institute for Radiological Protection | And 2 more authors.
Journal of Environmental Radioactivity | Year: 2012

Artificial Radiocaesium ( 137Cs) has been widely deposited over global soils. In this study, we measured 137Cs activity concentrations in the soil samples taken from Jing-Jin-Ji Region, North China, during 2007-2008. The surface soil (0-20cm) samples were collected from 452 sites in this region. The activity concentration of 137Cs in the samples was measured using a GEM series HPGe (high-purity germanium) coaxial detector system (ADCOM-100). The main findings are as following. (1) The activity concentration of 137Cs in surface soils in the region of study fluctuates within 0.3±0.1 to 12.9±0.4Bq/kg with mean value of 3.7Bq/kg. Compared to other regions located at roughly the same latitude, the 137Cs activity is relatively low. The results indicate that there are no new inputs of the radionuclides into the area at that time and the data obtained could serve as baseline levels of 137Cs in Jing-Jin-Ji Region. (2) Considering land use types, surface soil in woodland mostly shows high 137Cs activities with rare natural erosion and anthropogenic activities, and the surface soil with relatively low 137Cs activities appears in the areas of intensive anthropogenic activities, especially in the cities, river banks, reservoirs and the strongly eroded areas. The findings suggest that the varying distribution of 137Cs activities in surface soil corresponds with land use types. © 2012 Elsevier Ltd.

Wang X.-C.,Chinese Academy of Sciences | Tian L.-L.,Centers for Disease Control and Prevention | Jiang X.-Y.,Chinese National Institute for Radiological Protection | Wang Y.-Y.,Chinese Academy of Sciences | And 4 more authors.
Cancer Letters | Year: 2011

miRNAs are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional and translational levels. miRNA-451 was previously reported to be down-regulated in gastric and colorectal cancers. Here, we showed that miRNA-451 expression decreased in non-small cell lung cancer (NSCLC) tissues and that its expression was negatively associated with lymph node metastasis, the stage of TNM classification and poor prognosis of NSCLC patients. Moreover, significantly different miRNA-451 expression levels were found between smoking and non-smoking patients. The overexpression of miRNA-451 inhibited cell cycle progression, cellular migration and the invasive ability of NSCLC cells. Increased miRNA-451 expression also promoted anoikis of NSCLC cells. Together, these data suggested that aberrantly expressed miRNA-451 may be associated with the development of NSCLC. © 2011 Elsevier Ireland Ltd.

Wang X.-C.,Chinese Academy of Sciences | Tian L.-L.,Centers for Disease Control and Prevention | Tian J.,Cancer Prevention and Treatment Institute of Shandong Province | Jiang X.-Y.,Chinese National Institute for Radiological Protection
Radiation Research | Year: 2012

SKP2 is the substrate recognition subunit of the SCF SKP2 ubiquitin ligase complex. It is implicated in ubiquitin-mediated degradation of the cyclin-dependent kinase (CDK) inhibitor p27 KIP1 and positively regulates the G1/S transition. Overexpression of SKP2 has been found in many kinds of tumors. In the present study, we found that SKP2 expression levels increased in esophageal squamous cell carcinoma tissues. Elevated expression of SKP2 correlated significantly with tumor stage and positive lymph node metastasis (P < 0.05). Moreover, a significantly negative correlation was found between SKP2 expression and the survival of patients who received radiotherapy (P < 0.05). At the molecular level, induced expression of SKP2 promoted the radioresistance of EC9706 cells. Knockdown of SKP2 expression sensitized cancer cells to radiation, and a wobble mutant of SKP2 that was resistant to SKP2 siRNA was able to rescue this effect. Increased or decreased expression levels of SKP2 had effects on Rad51 expression after irradiation. These results demonstrate for the first time that overexpression of SKP2 was correlated with the increased radioresistance of esophageal squamous cell carcinoma. Elevated expression of SKP2 promoted the radioresistance of cancer cells, and this effect was mediated at least in part by the Rad51 pathway. © 2012 by Radiation Research Society.

Fu X.M.,Beijing University of Technology | Fu X.M.,Chinese National Institute for Radiological Protection | Xu F.R.,Beijing University of Technology | Jiao C.F.,Beijing University of Technology | And 3 more authors.
Physical Review C - Nuclear Physics | Year: 2014

Motivated by new experimental spectra in transfermium mass region, we have investigated broken-pair high-K multiparticle excited states and their rotational bands for the N=150 isotones around Z=100, by using the configuration-constrained pairing-deformation-frequency self-consistent total-Routhian-surface (TRS) model. In order to avoid the spurious phase transition encountered in the Bardeen-Copper-Schrieffer (BCS) pairing, the particle-number-conserving method has been employed for pairing calculations. Pairing correlations are remarkably reduced for the rotations of broken-pair multiparticle states. The present configuration-constrained TRS calculations reproduce reasonably existing experimental data. The abnormal feature in the recently observed Kπ=8- bands of No252 and Fm250 would be associated with configuration mixing. © 2014 American Physical Society.

Song Y.,CAS Beijing National Laboratory for Molecular | Song Y.,Chinese National Institute for Radiological Protection | Wang Z.,CAS Beijing National Laboratory for Molecular | Li L.,CAS Beijing National Laboratory for Molecular | And 3 more authors.
Chemical Communications | Year: 2014

Gold nanoparticles are functionalized as a nanoprobe with cresyl violet and porphyrin via hyaluronic acid. The nanoprobe becomes highly fluorescent in the presence of hyaluronidase or under ultraviolet irradiation, and can be used to target cancer cells via the overexpressed CD44 receptor for fluorescence imaging and phototherapy. © 2014 The Royal Society of Chemistry.

Yu Z.,Harvard University | Liu N.,Harvard University | Liu J.,Chinese National Institute for Radiological Protection | Yang K.,Harvard University | Wang X.,Harvard University
International Journal of Molecular Sciences | Year: 2012

Brain neurons and tissues respond to sublethal injury by activating endogenous protective pathways. Recently, following the failure of a large number of clinical trials for protective strategies against stroke that aim to inhibit a specific ischemia response pathway, endogenous neuroprotection has emerged as a more promising and hopeful strategy for development of therapeutics against stroke and neurodegenerative disorders. Neuroglobin (Ngb) is an oxygen-binding globin protein that is highly and specifically expressed in brain neurons. Accumulating evidence have clearly demonstrated that Ngb is an endogenous neuroprotective molecule against hypoxic/ischemic and oxidative stress-related insults in cultured neurons and animals, as well as neurodegenerative disorders such as Alzheimer's disease, thus any pharmacological strategy that can up-regulate endogenous Ngb expression may lead to novel therapeutics against these brain disorders. In this review, we summarize recent studies about the biological function, regulation of gene expression, and neuroprotective mechanisms of Ngb. Furthermore, strategies for identification of chemical compounds that can up-regulate endogenous Ngb expression for neuroprotection against stroke and neurodegenerative disorders are discussed. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Zhang W.,Chinese National Institute for Radiological Protection | Li X.,Chinese National Institute for Radiological Protection | Xu Z.,Security First
Procedia Engineering | Year: 2010

The study object in this research is the attenuation coefficient of linear (ACOL) from an interaction,which is get form the incident γ ray, radiated by a 600 keV (γ ray energy level is Near 137Cs) source and A 1250 keV (γ ray energy is equal to 60Co) source which are promoted by American NIST,interates with No.1No.92 element. After further research on two groups ACOL of interaction of rays and different meterials, we built a ratio of the ACOL of dual energy γ ray system(it is defined as Mz value). Then, we studied the relations of the ratio and material atom number. We get a quite good linear correlation value of a ACOL Mz of elements No.1 to No.92 and atom number. And, ten kinds of pure materials were choosed to test the ACOL Mz value, and we find the value can be located in the elemental distribution curve area. This rule promoted a important theory basis for the realization of dual energy CT technology which is widely used in container safety inspection. © 2010 Published by Elsevier Ltd.

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