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Hao R.-J.,Beijing Forestry University | Hao R.-J.,Shanxi Agricultural University | Zhang Q.,Beijing Forestry University | Zhang Q.,Agricultural University of Hebei | And 10 more authors.
Biochemical Systematics and Ecology | Year: 2014

Prunus mume is the only species of Prunus known to produce a strong floral fragrance. Most interspecific hybrids between P. mume and other species of Prunus lack the fragrance. The analysis of variations in emitted and endogenous compounds among genetically close cultivars is a powerful approach for revealing the mechanisms underlying floral scent emission. Compounds emitted by flowers from five cultivars were collected using the static headspace method, and endogenous compounds in the flowers were extracted with ethyl acetate. Samples were analysed quantitatively and qualitatively using gas chromatography-mass spectrometry. The result showed that benzenoids were the dominant compounds, of which benzyl acetate was the principal component contributing to the floral scent of P. mume. A clustering analysis of the floral volatiles from the different cultivars suggested that the scent traits of hybrids are related to the taxonomic relationship between their parents. The correlations between the amount of the endogenous and emitted compounds revealed that benzyl acetate had a stronger tendency to be volatile than the other compounds and the volatilisation rate of volatile compounds varied greatly among different cultivars. The importance of the biosynthetic pathway and the function of benzaldehyde are discussed. © 2014 Elsevier Ltd.

Kong Y.,Beijing Forestry University | Kong Y.,Beijing Radiation Center | Sun M.,Beijing Forestry University | Sun M.,Chinese National Engineering Research Center for Floriculture | And 4 more authors.
Journal of the American Society for Horticultural Science | Year: 2012

Floral scents emitted from eight cultivars of cut lily flowers (Lilium) were analyzed. Floral volatiles were collected by headspace adsorption on sorbent tubes and analyzed by gas chromatography-mass spectrometry (GC/ MS) using a direct thermal desorption. Fifty volatile compounds were identified. Nine compounds were detected in all lilies, whereas 20 compounds were detected in all scented lilies. The results revealed that non-scented lilies emitted trace amounts of volatile compounds, whereas scented lilies emitted high levels of volatile compounds. Monoterpenoids and benzenoids were the dominant compound classes of volatiles emitted from scented lilies. Myrcene, (E)- b-ocimene, linalool, methyl benzoate, and ethyl benzoate were the major compounds of the aroma of scented lilies; 1,8-cineole was also a major compound in the two scented oriental · trumpet hybrid lilies. Scent emissions occurred in a circadian rhythm with higher levels of volatiles emitted during the night. Lilium 'Siberia' was selected as a model to investigate the source of the emissions. GC/MS analysis of four flower parts and neutral red staining revealed that tepals were the source of floral scent.

Kong Y.,Beijing Forestry University | Kong Y.,Chinese National Engineering Research Center for Floriculture | Sun M.,Beijing Forestry University | Sun M.,Chinese National Engineering Research Center for Floriculture | And 4 more authors.
Asian Journal of Chemistry | Year: 2012

Headspace solid-phase microextraction (HS-SPME) and direct thermal desorption (DTD) coupled to gas chromatography with mass spectrometry (GC/MS) were used to determine the floral scent composition of Lilium 'Siberia'. Three types of fiber coating 100 μm PDMS, 2 cm-50/30 μm DVB/CAR/PDMS and 75 μm CAR/PDMS were employed for solid-phase microextraction sampling and Tenax GR was used as adsorbent for direct thermal desorption. The results demonstrated that the major floral scent components were monoterpenoids (73.78-98.52 %) and benzenoids (1.09-25.98 %). Linalool, (E)-b-ocimene and methyl benzoate were the most major components. Their total relative amounts were 83.28-97.92 %. In the tests, 26 compounds were separated by DTD-GC/MS, more than the other sampling mediums. As direct thermal desorption provided a more flexible sampling site. We considered that direct thermal desorption with Tenax GR absorbent is more suitable for Lilium flower fragrance sampling.

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