Chinese Institute of Microbiology and Epidemiology

Beijing, China

Chinese Institute of Microbiology and Epidemiology

Beijing, China
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Wu Y.R.,Chinese Institute of Microbiology and Epidemiology
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2016

Population genomics, an interdiscipline of genomics and population genetics, is booming in recent years with the rapid growth number of deciphered genomes and revolutionizes the understanding of bacterial population diversity and evolution dynamics. It also largely improves the prevention and control of infectious disease through providing more accurate genotyping and source-tracing results and more comprehensive characteristics of emerging pathogens. In this review, taking one of the best characterized bacteria, Escherichia coli, as model, we reviewed the phylogenetic relationship across its five major populations (designated A, B1, B2, D and E); and summarized researches on molecular mutation rate, selection signals, and patterns of adaptive evolution. We also described the application of population genomics in responding against large-scale outbreaks of E. coli O157:H7 and E. coli O104:H4. These results indicated that, although being a novel discipline, population genomics has played an important role in deciphering bacterial population structures, exploring evolutionary patterns and combating emerging infectious diseases.


Han Y.H.,Chinese Institute of Microbiology and Epidemiology
Human vaccines | Year: 2011

We used site-directed mutagenesis to mutate two key amino acid residues, Glu164 and Arg167, of abrin A chain (ABRA), creating a mutant ABRA(E164AR167L). The mutant ABRA(mABRA) encoded by mABRA(E164AR167L) was expressed in the cytoplasm of Escherichia coli, and used to develop an effective vaccine to protect mice against native abrin intoxication. The cytotoxicity of mABRA was dramatically reduced as compared to that of recombinant ABRA(rABRA) and native abrin, but the antigenicity and immunogenicity remained the same. Balb/c mice were vaccinated with purified mABRA, and survival was evaluated after challenge with native abrin. Mice that were given three vaccinations developed a protective immune response that was 100% protective against an intraperitoneal (i.p.) administration of 10×LD50 of native abrin. Furthermore, the sera from immunized mice provided complete passive protection for naive mice. This study describes the generation of a substantial amount of mABRA from E. coli and the potential application of mABRA as an effective vaccine candidate for humans, to protect against a high-dose of native abrin.


Jin-Song L.,Chinese Institute of Microbiology and Epidemiology
Journal of International Pharmaceutical Research | Year: 2015

Ebola virus is a highly virulent pathogen causing severe hemorrhagic fever with a high fatality rate in humans. Although safe and effective therapeutics and treatment strategies or other medicinal agents in post-exposure therapeutics for the prevention of Ebola hemorrhagic are currently unavailable, a significant effort has been put forth to identify several promising candidates for post-exposure therapeutics and treatment of Ebola hemorrhagic fever. These potential strategies and novel technology include monoclonal antibody cocktail, polymerase inhibitors and lipid nanoparticle/small interfering RNA. This article summarizes recent advances in therapeutics and treatment strategies and novel technology in Ebola virus post-infection in small animals and non-human primates. © 2015, Editorial office of Journal of International Pharmaceutical Research. All rights reserved.


Liu G.,Fudan University | Bi Y.,Chinese Institute of Microbiology and Epidemiology | Wang R.,St Jude Childrens Research Hospital | Wang X.,Shanghai JiaoTong University
Journal of Leukocyte Biology | Year: 2013

Autophagy (macroautophagy; "self-eating") is a degradation process, in which cytoplasmic content is engulfed and degraded by the lysosome. And, immunity is an important mechanism of the "self-defense" system. Autophagy has long been recognized as a stress response to nutrient deprivation. This will provide energy and anabolic building blocks to maintain cellular bioenergetic homeostasis. Thus, autophagy plays critical roles in regulating a wide variety of pathophysiological processes, including tumorigenesis, embryo development, tissue remodeling, and most recently, immunity. The latter shows that a self-eating (autophagy) process could regulate a self-defense (immune) system. In this review, we summarize the recent findings regarding the regulatory and mechanistic insights of the autophagy pathway in immunity. © Society for Leukocyte Biology.


Patent
Chinese Institute of Microbiology, Epidemiology and Shanghai Gongtai Textile Products Co. | Date: 2011-10-26

Disclosed is an insect repellant fabric and a method for preparing the same, wherein, the method for preparing the insect repellant comprises the following steps: making a fabric composed of a lining layer made of natural fibers, an outshell layer made of synthetic fiber multifilaments, and a lapping layer made of polypropylene or polyethylene fiber monofilaments, and both the lining layer and the outshell layer are made as a mesh structure; dipping the prepared fabric into an exhaust dyeing solution and being dyed for 10 to 120 minutes at a temperature of 60 to 105C; water rinsing, centrifuge drying and setting. Wherein, the exhaust dyeing solution is an aqueous solution containing emulsifiable concentrate, the emulsifiable concentrate is a mixture of pyrethroids and an alcohol-ether solvent in a mass ratio of 1:5 to 1:20; the mass ratio of the fabric to the exhaust dyeing solution is 1:7 to 1:20, and the amount of the emulsifiable solution is 5 to 10% based on the mass of the fabric. The method of the present invention has advantages such as a short processing flow, easy operation and low dosage of pyrethroids. In the method of the present invention, pesticides such as pyrethoroids are applied to the fabric by using exhaust dyeing process, which has a low requirement for the property and quality of the fibers. And the fabric prepared by using the method of the present invention shows good air permeability, strong defense function for insect invasion and permanent insect repellant effect.


Bi Y.,Chinese Institute of Microbiology and Epidemiology | Liu G.,CAS Institute of Zoology | Yang R.,Chinese Institute of Microbiology and Epidemiology
Journal of Cellular Physiology | Year: 2011

T helper 17 (TH17) cells have well-described roles in autoimmune disease. However, TH17 is not stable in some physiological or pathological courses. Also, TH17 cells can reciprocally modulate and convert into other helper T cell subpopulations. The fully exploring the reciprocal regulatory effects and its immunoregulatory mechanisms are becoming interesting topics in the immunological study. In this review, we summarized reciprocal modulation pattern between TH17 cell and other helper T cell subpopulations in the mouse model of autoimmune diseases and human diseases. © 2010 Wiley-Liss, Inc.


Patent
Chinese Institute of Microbiology, Epidemiology and The Second Military Medical University | Date: 2016-03-30

The present invention discloses a mercaptonicotinic acid compound and preparation method and use thereof; the structural formula of the compound is as shown in Formula I. Experiment demonstrates that the mercaptonicotinic acid compound inhibits in vitro botulinum toxin endopeptidase activity and Clostridium tetanus toxin endopeptidase activity, has a significant protective effect on botulinum toxin poisoning, and can be used for the prevention and/or treatment of botulism and prevention of botulinum toxin-exposure drugs and tetanus drugs.


Patent
Chinese Institute of Microbiology, Epidemiology and The Second Military Medical University | Date: 2014-05-19

The present invention discloses a class of mercaptonicotinic acid compound and preparation method and use thereof; the general structural formula of the compound is as shown in Formula I. Experiment demonstrates that the mercaptonicotinic acid compound could inhibit activity of botulinum toxin endopeptidase and tetanus toxin endopeptidase in vitro, which has a significant protective effect on botulinum toxin poisoning in mice, and can be used for preparing medicine intended to prevent and/or treat botulism, as well as to prevent botulinum toxin exposure and tetanus.


Patent
Chinese Institute of Microbiology and Epidemiology | Date: 2013-07-31

The present invention provides a polypeptide TF1 for inhibiting type-2 Shiga-toxin activity, an encoding gene for the same and use thereof. The present polypeptide is named TF1 (also known as P1); its amino acid sequence is shown in Sequence 1 in the sequence list. The polypeptide P1 can be prepared into medicine for preventing and/or treating diseases caused by type-2 Shiga toxin or the pathogens which produce type-2 Shiga toxin.


Patent
Chinese Institute of Microbiology and Epidemiology | Date: 2013-07-31

The present invention discloses a fusion protein SAmB, a coding gene and an application thereof. The fusion protein provided in the present invention is named SAmB, comprising on the N-terminal a Shiga toxin type-II A subunit mutant protein and on the C-terminal a Shiga toxin type-I B subunit protein. The Shiga toxin type-II A subunit mutant protein is prepared by mutating the active site amino acid residues of Shiga toxin type-II A subunit protein. The fusion protein SAmB provided in the present invention can be used to prepare a medicament for use in the prevetion and/or treatment of a Shiga toxin pathogenic bacteria infection or complication of the infection.

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