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Liu G.,Fudan University | Bi Y.,Chinese Institute of Microbiology and Epidemiology | Wang R.,St Jude Childrens Research Hospital | Wang X.,Shanghai JiaoTong University
Journal of Leukocyte Biology | Year: 2013

Autophagy (macroautophagy; "self-eating") is a degradation process, in which cytoplasmic content is engulfed and degraded by the lysosome. And, immunity is an important mechanism of the "self-defense" system. Autophagy has long been recognized as a stress response to nutrient deprivation. This will provide energy and anabolic building blocks to maintain cellular bioenergetic homeostasis. Thus, autophagy plays critical roles in regulating a wide variety of pathophysiological processes, including tumorigenesis, embryo development, tissue remodeling, and most recently, immunity. The latter shows that a self-eating (autophagy) process could regulate a self-defense (immune) system. In this review, we summarize the recent findings regarding the regulatory and mechanistic insights of the autophagy pathway in immunity. © Society for Leukocyte Biology.


Patent
Chinese Institute of Microbiology, Epidemiology and Shanghai Gongtai Textile Products Co. | Date: 2011-10-26

Disclosed is an insect repellant fabric and a method for preparing the same, wherein, the method for preparing the insect repellant comprises the following steps: making a fabric composed of a lining layer made of natural fibers, an outshell layer made of synthetic fiber multifilaments, and a lapping layer made of polypropylene or polyethylene fiber monofilaments, and both the lining layer and the outshell layer are made as a mesh structure; dipping the prepared fabric into an exhaust dyeing solution and being dyed for 10 to 120 minutes at a temperature of 60 to 105C; water rinsing, centrifuge drying and setting. Wherein, the exhaust dyeing solution is an aqueous solution containing emulsifiable concentrate, the emulsifiable concentrate is a mixture of pyrethroids and an alcohol-ether solvent in a mass ratio of 1:5 to 1:20; the mass ratio of the fabric to the exhaust dyeing solution is 1:7 to 1:20, and the amount of the emulsifiable solution is 5 to 10% based on the mass of the fabric. The method of the present invention has advantages such as a short processing flow, easy operation and low dosage of pyrethroids. In the method of the present invention, pesticides such as pyrethoroids are applied to the fabric by using exhaust dyeing process, which has a low requirement for the property and quality of the fibers. And the fabric prepared by using the method of the present invention shows good air permeability, strong defense function for insect invasion and permanent insect repellant effect.


Bi Y.,Chinese Institute of Microbiology and Epidemiology | Liu G.,CAS Institute of Zoology | Yang R.,Chinese Institute of Microbiology and Epidemiology
Journal of Cellular Physiology | Year: 2011

T helper 17 (TH17) cells have well-described roles in autoimmune disease. However, TH17 is not stable in some physiological or pathological courses. Also, TH17 cells can reciprocally modulate and convert into other helper T cell subpopulations. The fully exploring the reciprocal regulatory effects and its immunoregulatory mechanisms are becoming interesting topics in the immunological study. In this review, we summarized reciprocal modulation pattern between TH17 cell and other helper T cell subpopulations in the mouse model of autoimmune diseases and human diseases. © 2010 Wiley-Liss, Inc.


Bi Y.,Chinese Institute of Microbiology and Epidemiology | Liu G.,CAS Institute of Zoology | Yang R.,Chinese Institute of Microbiology and Epidemiology
Critical Reviews in Eukaryotic Gene Expression | Year: 2011

mTOR is an evolutionarily conserved PI3-kinase family member that plays a central role in integrating environmental cues in the form of amino acids, energy, and growth factors. Recently, the kinase mTOR has emerged as an important regulator of the differentiation and function of helper T cells in immune responses and autoimmune diseases. In this review, we summarize the regulatory effects and mechanisms of mTOR complex in the differentiation of CD4 +T and CD8 + T cells, and T-cell homeostasis modulation. This should contribute to understanding the central role for mTOR in regulating immune responses and autoimmune diseases. © 2011 Begell House.


Li S.S.,Chinese Institute of Microbiology and Epidemiology
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] | Year: 2013

To confirm the hypothesis that the high frequency sequences of high throughput sequencing are the terminal sequences of the bacteriophage genome. An adaptor of specific sequence was linked to the end of the bacteriophage T3 genomic DNA, which was then subject to high throughput sequencing; as a control, the same T3 genomic DNA without adaptor was also analyzed by high throughput sequencing. The sequencing results were examined with bioinformatics software. Similar high throughput sequencing technique was applied to analyze the genomic sequence of N4-like bacteriophage IME11. Bioinformatics study showed that the sequences tagged with adaptors were consistent with the high frequency sequences without adaptor labeling. Our analysis also indicated that the end of the T4-like phage genome had specific sequences instead of random sequences, disagreeing with the previous assertion. Evidences were provided that N4-like bacteriophage had a particular terminal sequence: the left end of the genome was unique while the right end was permuted. The high throughput sequencing technique was convenient and practical to be used to simultaneously detect the terminal sequence and the complete sequence of bacteriophage genome.


Ke Y.,Academy of Military Medical science | Ke Y.,Chinese Institute of Microbiology and Epidemiology | Chen Z.,Academy of Military Medical science | Yang R.,Chinese Institute of Microbiology and Epidemiology
Frontiers in Cellular and Infection Microbiology | Year: 2013

During infection, Yersinia, a facultative intracellular bacterial species, exhibits the ability to first invade host cells and then counteract phagocytosis by the host cells. During these two distinct stages, invasion or antiphagocytic factors assist bacteria in manipulating host cells to accomplish each of these functions; however, the mechanism through which Yersinia regulates these functions during each step remains unclear. Here, we discuss those factors that seem to function reversely and give some hypothesis about how bacteria switch between the two distinct status. © 2013 Ke, Chen and Yang.


Patent
Chinese Institute of Microbiology, Epidemiology and The Second Military Medical University | Date: 2016-03-30

The present invention discloses a mercaptonicotinic acid compound and preparation method and use thereof; the structural formula of the compound is as shown in Formula I. Experiment demonstrates that the mercaptonicotinic acid compound inhibits in vitro botulinum toxin endopeptidase activity and Clostridium tetanus toxin endopeptidase activity, has a significant protective effect on botulinum toxin poisoning, and can be used for the prevention and/or treatment of botulism and prevention of botulinum toxin-exposure drugs and tetanus drugs.


Patent
Chinese Institute of Microbiology, Epidemiology and The Second Military Medical University | Date: 2014-05-19

The present invention discloses a class of mercaptonicotinic acid compound and preparation method and use thereof; the general structural formula of the compound is as shown in Formula I. Experiment demonstrates that the mercaptonicotinic acid compound could inhibit activity of botulinum toxin endopeptidase and tetanus toxin endopeptidase in vitro, which has a significant protective effect on botulinum toxin poisoning in mice, and can be used for preparing medicine intended to prevent and/or treat botulism, as well as to prevent botulinum toxin exposure and tetanus.


Patent
Chinese Institute of Microbiology and Epidemiology | Date: 2013-07-31

The present invention provides a polypeptide TF1 for inhibiting type-2 Shiga-toxin activity, an encoding gene for the same and use thereof. The present polypeptide is named TF1 (also known as P1); its amino acid sequence is shown in Sequence 1 in the sequence list. The polypeptide P1 can be prepared into medicine for preventing and/or treating diseases caused by type-2 Shiga toxin or the pathogens which produce type-2 Shiga toxin.


Patent
Chinese Institute of Microbiology and Epidemiology | Date: 2013-07-31

The present invention discloses a fusion protein SAmB, a coding gene and an application thereof. The fusion protein provided in the present invention is named SAmB, comprising on the N-terminal a Shiga toxin type-II A subunit mutant protein and on the C-terminal a Shiga toxin type-I B subunit protein. The Shiga toxin type-II A subunit mutant protein is prepared by mutating the active site amino acid residues of Shiga toxin type-II A subunit protein. The fusion protein SAmB provided in the present invention can be used to prepare a medicament for use in the prevetion and/or treatment of a Shiga toxin pathogenic bacteria infection or complication of the infection.

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