Chinese Astronaut Science Researching and Training Center

Beijing, China

Chinese Astronaut Science Researching and Training Center

Beijing, China

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Wu J.,Beijing University of Technology | Zhang T.,Beijing University of Technology | Wang C.,Chinese Astronaut Science Researching and Training Center | Chen T.,Beijing University of Technology
Current Nanoscience | Year: 2017

Background: Microfluidic chip, also known as lab-on-chip, is recognized as one of the most important advanced science and technology in twenty-first Century. Due to high chemical specificity and high information content, SERS can be used in a microfluidic channel to achieve simultaneous monitoring of composition of multiple analytes. In this paper, we present a simple method to prepare SERS substrate inside a microfluidic chip to detect SYBR Green I. Methods: A microfluidic chip was prepared by a 248 nm excimer laser. A particle-free and lowviscosity precursor solution synthesized by silver acetate, formic acid, and aqueous ammonium hydroxide was dropped in the microfluidic channel. After fifteen minutes of heating on a heating platform, Ag nanoparticles were generated in the channel of microfluidic chip. Then the SERS-based microfluidic systems was used to detect the SYBR Green I dye molecules. Results: Raman signal of SYBR Green I dye can be significantly enhanced in this system. Moreover, SYBR Green I could be quantitatively detected using this system by measuring the SERS signal. Conclusion: In this paper, a method for rapid and in situ fabrication of Ag nano materials in microfluidic chip was presented to detect SYBR Green I by SERS. This method is simple and fast. It has good research and application prospects in polymerase chain reaction (PCR) detection or biological and pharmaceutical testing. © 2017 Bentham Science Publishers.


Wu J.,Beijing University of Technology | Guo W.,Beijing University of Technology | Wang C.,Chinese Astronaut Science Researching and Training Center | Li Y.,Chinese Astronaut Science Researching and Training Center | And 2 more authors.
Surface Engineering | Year: 2015

In order to improve surface roughness and hydrophilicity of microchannels in microfluidic chip, then to achieve high fluid speed under small pressure, a technology named as coating solidifiable adhesive film is proposed. In this paper the microfluidic chip is manufactured using CO2 laser. The film is coated on inside wall of microchannels using ultraviolet optic solidifiable adhesive. Two coating methods were used to synthesise the NOA61 film. The surface roughness and waterphase contact angle of the microchannel with and without the NOA61 film are measured. The roughness is reduced from 2 μm to 243·36 nm and the contact angle is reduced from 100 to 72·55°, when NOA61 film is coated with the action of gas blowing and consequent drying by UV light. The roughness is reduced from 2 μm to 60·49 nm and the contact angle is reduced from 100 to 55·35u, when NOA61 film is obtained by gravity flow. © 2015 Institute of Materials, Minerals and Mining.


Wu J.,Beijing University of Technology | Yu K.,Beijing University of Technology | Chen T.,Beijing University of Technology | Li Y.,Chinese Astronaut Science Researching and Training Center | Wang C.,Chinese Astronaut Science Researching and Training Center
Laser and Tera-Hertz Science and Technology, LTST 2012 | Year: 2012

A new manufacture technology of micro-lens generated on needing positions is suggested. A micro-detector to fluorescence spectrum using the micro-lens is made. Experiment results indicatethat the micro-detection with the micro-lens can increase sensitivity greatly. © 2012 OSA.


Wu J.,Beijing University of Technology | Yu K.,Beijing University of Technology | Chen T.,Beijing University of Technology | Li Y.,Chinese Astronaut Science Researching and Training Center | Wang C.,Chinese Astronaut Science Researching and Training Center
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

In this paper a new manufacture technology of the micro-lens generated on needing positions is suggested. This technology doesn't need mould or masks, it generates the micro-lens on the needing positions directly according to needing curve shape. It is a simple and quick laser micro-manufacture technology, which solves the difficulties of high accurate separation the micro-lens from substrate and adhesion the micro-lens to working face coaxially. A microdetector to fluorescence spectrum using the micro-lens generated on needing positions is made. The detector has many advantages, for example high sensitivity and micro volume. It can be inserted in biochip, and it is used in alarm system of harmful microbes in spacecraft also. © 2012 SPIE.


Wu J.,Beijing University of Technology | Liu W.-C.,Beijing University of Technology | Yu K.-X.,Beijing University of Technology | Chen T.,Beijing University of Technology | And 2 more authors.
Journal of Applied Optics | Year: 2013

In order to solve the difficulty for manufacture of micro-lens in micro-detector of fluorescent spectrum, a new manufacture technique of micro-lens, molded on needing position, was proposed. Light gathering ability of the optic micro-lens was researched. Using principles of traditional geometric optics, ray tracking equations of stimulating micro-lens and detecting micro-lens were derived. Distributed probabilities of illumination points for the two micro-lenses were calculated by using these equations. Some corresponding distributed curves were given. Influence of the micro-lens's shape on the light gathering ability was discussed. Results indicate that the contact angle between optic glue and glass base can be decreased and the shape parameter of the micro-lens can be increased by adulteration in the optic glue and milling the glass. It can both strengthen adhesiveness between the glue and the glass, and increase light gathering ability of the micro-lens. A micro-detector for fluorescent spectrum with micro-lens molded on needing position was manufactured and the biological fluorescent reagent was measured. Experiment results indicate that the intensity of the biologic fluorescent spectrum can be increased more than 4 times by using micro-lens molded on needing position, the light gathering ability of the micro-lens can be increased 1.2 times though adulteration in the glue and milling the glass.


Wu J.,Beijing University of Technology | Guo W.,Beijing University of Technology | Wang C.,Chinese Astronaut Science Researching and Training Center | Yu K.,Beijing University of Technology | And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2015

Fluorescent polymerase chain reaction (PCR) is becoming the preferred method of quantitative analysis due to its high specificity and sensitivity. We propose to use a new kind of micro-lens, dynamically solidified with optic glue, to improve the sensitivity of fluorescent PCR micro-detector. We developed light ray track equations for these lenses and used them to calculate relative light intensity distribution curve for stimulation lenses and illumination point probability distribution curve for detection lenses. We manufactured dynamically solidified micro-lenses using optic glue NOA61, and measured their light gathering ability. Lenses with radius/thickness (R/H) ratio of 4 reached light focusing ratio of 3.85 (stimulation lens) and photon collection efficiency of 0.86 (detection lens). We then used dynamically solidified lenses in PCR fluorescence micro-detector and analyzed their effect on the detector sensitivity. We showed that the use of dynamically solidified micro-lenses with R/H = 4 resulted in over 4.4-fold increased sensitivity of the detector. © 2015, Springer Science+Business Media New York.


Wu J.,Beijing University of Technology | He H.-Y.,Beijing University of Technology | Yu K.-X.,Beijing University of Technology | Chen T.,Beijing University of Technology | And 2 more authors.
Beijing Gongye Daxue Xuebao/Journal of Beijing University of Technology | Year: 2013

Fluid mechanics principle about the optic micro-lens generated on needing positions is researched. A set of surface equations of optic glue drip, which spreads on horizontal plane under surface tention, is given. The equations set reflect relationships between coordinates of an arbitary point on the surface. A numerical calculation method of the equations set is proposed. Some surface shape parameters of the glue drip are calculated for different performance parameters, such as volume, contact angle and character length. Some shape curves and curvature radius curves are given. Results show that the contact angle and character length decrease, spread radius increase and thickness decrease, and non-sphere performance is strengthened. The intensity of the micro lens increases and the contact angle and character length decrease by mixing appropriate amount nanometer quartz powder into the glue. The bond strength between the glue and the glass increases and the contact angle decreases by milling or roughing the glass face.


Wu J.,Beijing University of Technology | Yang Y.,Beijing University of Technology | Yu K.,Beijing University of Technology | Chen T.,Beijing University of Technology | And 2 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

In this paper a new technology of optic micro lens generated on needing positions is proposed. It is formed directly on positions where the mocro-lens is mounted. The technique is easy, quick and cheap. It has application prospects in astronavigation, military affairs, biology, chemistry and civil affair. In this paper fluid mechanics principle about the optic micro lens generated on needing positions is researched. A surface equation set of optic glue drip, which spreads on horizontal plane under surface tention, is given. The equation set reflect relations between coordinates of the surface curve. A numerical calculation method of the equation set is proposed. Some shape curves and curvature radius curves for different character parameters of the glu drip are given. Influences on shape of the micro lens through mixing nanometer quartz powder in the glue drip and milling the glass are discussed. © 2012 SPIE.


PubMed | Beijing University of Technology and Chinese Astronaut Science Researching and Training Center
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

Fluorescent polymerase chain reaction (PCR) is becoming the preferred method of quantitative analysis due to its high specificity and sensitivity. We propose to use a new kind of micro-lens, dynamically solidified with optic glue, to improve the sensitivity of fluorescent PCR micro-detector. We developed light ray track equations for these lenses and used them to calculate relative light intensity distribution curve for stimulation lenses and illumination point probability distribution curve for detection lenses. We manufactured dynamically solidified micro-lenses using optic glue NOA61, and measured their light gathering ability. Lenses with radius/thickness (R/H) ratio of 4 reached light focusing ratio of 3.85 (stimulation lens) and photon collection efficiency of 0.86 (detection lens). We then used dynamically solidified lenses in PCR fluorescence micro-detector and analyzed their effect on the detector sensitivity. We showed that the use of dynamically solidified micro-lenses with R/H=4 resulted in over 4.4-fold increased sensitivity of the detector.


PubMed | Beijing University of Technology and Chinese Astronaut Science Researching and Training Center
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

In this study, a Norland optical adhesive 68 (NOA68) film, approximately 2.2 m thick, was manufactured using ultraviolet solidified NOA68 in apparatus manufacturing film on the inwall of a capillary copper pipe, developed in our laboratory. The roughness of the inwall of capillary copper pipe was improved from Ra = 0.766 to 0.204 m and the contact angle was improved from approximately 96 to 55, increasing hydrophilicity. Polymerase chain reaction experiments indicated that the ratio of work pressure in the microfluidic chip before modification to that after modification was 2.71/1, indicating that the extension efficiency increased. Our results provide a basis for the construction of a microform chip based on function integration.

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