Chinese Academy of Fishery Sciences

Shanghai, China

Chinese Academy of Fishery Sciences

Shanghai, China
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News Article | May 5, 2017

The Food and Agriculture Organization of the United Nations (FAO) in cooperation with the Brazil's Ministry of Industry, Foreign Trade and Services/MDIC, The Ministry of Agriculture, Livestock and Food Supply/MAPA and the Brazilian Association of Shrimp Growers/ABCC, is tackling Infectious myonecrosis virus (IMNv), one of the most serious pathogens, affecting cultured whiteleg shrimp (Penaeus vannamei). Penaeus vannamei is a very important culture commodity with a global production worth almost USD 19 billion. This event, 4-day workshop, which started yesterday, is being carried out through an FAO Project "FAO – TCP/INT/3501: Strengthening biosecurity governance and capacities" for dealing with the serious shrimp infectious myonecrosis virus (IMNv) disease. The workshop is sharing information on Emergency Preparedness and Response Systems in the 6 participating countries and is developing a Disease Strategy Plan for IMNv. During the next three days, the workshop will prepare an active surveillance design for IMNv and a framework for developing a National Strategy on Aquatic Animal Health. The workshop will also deliberate on appropriate export/import sanitary measures/actions for live shrimp and shrimp products from countries that are free and countries that are not free from IMNv, as well as practical application of biosecurity zoning and maintenance of disease free status. Some 25 delegates representing China (Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences), Ecuador (National Fisheries Institute), Indonesia (Ministry of Marine Affairs and Fisheries), Mexico (National Health Service, Food Safety and Quality), Thailand (Department of Fisheries), FAO experts from Canada, Chile and the USA and local Brazilians representing the government, producer and academic sectors are participating in this important event. A half-day Technical Seminar for Aquaculture Stakeholders will also be held tomorrow to share country and expert experiences on important shrimp diseases and other emerging issues affecting aquaculture. Dr Rodrigo Roubach, in welcoming the delegates, pointed out the work that been developed by the Brazilian shrimp industry alongside with the government towards better governance and improving the management and health status of the shrimp sector, especially in facing the challenges posed by shrimp diseases. In this regard, the present FAO TCP project is extremely important for better understanding of these challenges and closer cooperation amongst the main world shrimp producers in order provide more efficient and systematic response to future disease outbreaks through improved sector governance.

Agency: European Commission | Branch: FP7 | Program: CP | Phase: ENV.2013.WATER INNO&DEMO-1 | Award Amount: 9.23M | Year: 2014

The objective of INAPRO is to mobilise industry, member states and stakeholders to promote a new and innovative technical and technological approach right up to an Aquaponic system which allows a nearly emission free sustainable production and contributes remarkably to global food security for the 21st century. Considering that traditional Aquaponic systems, combining aquaculture and hydroponics, have a great potential in saving water and energy and recovering nutrients from wastewater by value chains, the project aims at a real breakthrough for these systems towards commercialization. This will be achieved by a) the model based optimisation of the system concept in respect to water consumption and quality, environmental impact, waste avoidance, CO2 release and nutrient recycling, energy efficiency, management efforts and finally costs and b) the integration of new technologies containing cutting edge approaches such as: 1) innovative one-way water supply for horticulture and water retrieval by condensation, 2) alternative water and energy sources, 3) optimized filter systems, 4) intelligent sensor and management network for an optimized system construction and operation. The viability of INAPRO systems will be proved in concept-based demonstration projects both in rural and in urban areas that offer a potential economic advantage while simultaneously reducing water and carbon footprint. The dissemination activities (to policy, public and end-users) will open new market opportunities and improve market access inside and outside Europe for producers and technology suppliers. These ambitions meet perfectly with the EU strategies under Europe 2020 to face the challenges of dramatic water resource developments in Europe and worldwide. The project supports particularly the Innovation Union with the EIP Water as one key initiative and further the Common Agricultural Policy and will consequently be closely connected to an EIP Action Group in agricultural water management.

Agency: European Commission | Branch: H2020 | Program: RIA | Phase: SFS-11a-2014 | Award Amount: 3.63M | Year: 2015

The AquaSpace project has the goal of providing increased space for aquaculture to allow increased production. Following the call, we will achieve this by identifying the key constraints experienced by aquaculture development in a wide range of contexts and aquaculture types, taking into account all relevant factors and advised by a Reference User Group. We will then map these constraints against a wide variety of tools/methods that have already been developed in national and EU projects for spatial planning purposes, including some that have been designed specifically for aquaculture. In the freshwater sector only, we will also consider ecosystem services provided by aquaculture that are relevant to integrated catchment planning and management. At 16 case study sites having a variety of scales, aquaculture at different trophic levels with different environmental interactions and most importantly with a range of key space-related development constraints as defined by local stakeholders, we will assess appropriate tools using a common process so as to facilitate synthesis and comparison. This case study approach will generate a large amount of information and is allocated about a third of the projects resources. The project will develop the outcomes leading to a set of evaluated tools for facilitating the aquaculture planning process by overcoming present constraints. This information will be presented on an interactive web-based platform with tailored entry points for specific user types (e.g. planners, farmers, public) to enable them to navigate to the tools most appropriate to their application. The knowledge and information gained during this process will be developed into an on-line module at Masters Level which will also be developed into a short CPD course aimed at aquaculture planning professionals. The public will be engaged by an innovative school video competition and a vehicle to ensure project legacy will be established.

Agency: European Commission | Branch: FP7 | Program: CSA-CA | Phase: KBBE.2011.1.4-07 | Award Amount: 1.06M | Year: 2012

Aquaculture is widely considered as important for enhancing food security, alleviating poverty and improving nutrition. However, little information is available concerning the direct and indirect impacts of aquaculture on food security and poverty alleviation in most developing countries and LIFDCs. Strengthening the knowledge base surrounding aquaculture and food and nutrition security through this project will provide the evidence upon which sound resource allocation and strategies can be based, and subsequently plan, implement and coordinate efficiently development and research programmes supporting the sustainable expansion of aquaculture and increasing its impact to food security and poverty alleviation. The project is to be implemented by 18 partners in 11 selected LIFDCs, 3 EU partners, and 3 international organizations. The project will strengthen the knowledge base on food security and poverty and develop new methodologies or more rigorous methodologies to quantify the contribution of aquaculture in combating hunger and poverty in developing countries and LIFDCs. This will endeavour to better understand aquacultures contribution to human development. Project partner countries were selected based on varied human development conditions and national level efforts in including aquaculture for improving national food security and alleviating poverty. They represent all major aquaculture regions and ICPCs where aquaculture has major contributions to national economy involve high numbers of small-scale aquaculture farms, and with high international trade of fish and fishery products. The results of the project will be brought to the attention of countries and development partners, particularly the EU, and outputs will help LIFDCs and various development partners to improve efficiency and coordination in development initiatives focused on aquaculture as a means of promoting food security and poverty alleviation.

Tian J.T.,Chinese Academy of Fishery Sciences
Genetics and molecular research : GMR | Year: 2012

Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coastal areas of Qingdao, China. The values of observed heterozygosity, expected heterozygosity and polymorphism information content per locus ranged from 0.1034 to 0.9655, from 0.1831 to 0.9208, and from 0.1638 to 0.8964, respectively. Forty-three of 48 loci conformed to Hardy-Weinberg equilibrium. These microsatellite loci would be useful for molecular genetic breeding, population genetics, genome mapping, and other relevant research on S. broughtonii.

Sun X.,Chinese Academy of Fishery Sciences
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

We determined the phylogenetic position of a heterotrophic nitrifying-aerobic denitrifying bacterium X3, and detected its nitrogen removal characteristics for providing evidence to explain the principle of heterotrophic nitrification-aerobic denitrification and to improve the process in purification of marine-culture wastewater. The evolutionary position of the strain was determined based on its morphological, physiological, biochemical characteristics and 16SrRNA gene sequence. The nitrification-denitrification ability of this strain was detected by detecting its nitrogen removal efficiency and growth on different inorganic nitrogen source. Strain X3 was identified as Halomonas sp. It grew optimally at salinity 3%, pH 8.5, C:N 10:1 at 28 degrees C, and it could still survive at 15% salinity. The removal of NH4+ -N, NO2(-) -N and NO3(-) -N was 98.29%, 99.07%, 96.48% respectively within 24 h. When three inorganic nitrogen existed simultaneously, it always utilized ammonia firstly, and the total inorganic nitrogen removal was higher than with only one nitrogen, suggesting that strain X3 has the ability of simultaneous nitrification and denitrification and completing the whole nitrogen removing process. Strain X3 belonged to the genus of Halomonas. It had strong simultaneous nitrification and denitrification capability and could live in high-salinity environment.

Antimicrobial peptide plays an important role in fish immunity. The small molecular antimicrobial peptide Hepcidin in turbot was studied and reported in this paper. The Ferroportin 1 (FPN1) and Transferrin Receptor (TFR) genes, which are related to Hepcidin, were cloned in turbot. The characteristics of Hepcidin and its related genes were studied, including an analysis of the expression patterns and cloning of the Hepcidin promoter, the relationship between Hepcidin and NF-κB and the regulation of iron-metabolism. The results showed that the promoter of SmHepcidin contains the binding sites of NF-κB, and NF-κB may directly or indirectly receive feedback signals from SmHepcidin. In the liver, spleen and kidney, in which there was an increased SmHepcidin expression level, SmFPN1 dramatically decreased and SmTFR was also either decreased or exhibited no obvious change after bacterial/viral infection and an injection of exogenous Hepcidin protein. RNAi experiments in turbot kidney cells confirmed the expression changes of these gene patterns. Furthermore, the administration of exogenous Hepcidin protein, which regulates the level of chelatable iron in cells, further confirmed the function of Hepcidin in iron metabolism. It is speculated that the rapidly increased expression of SmHepcidin may induce changes in the expression of related genes, and that the in vivo chelatable iron concentration which participates in the antibacterial process was also changed when exogenous pathogens are present in turbot. It is suggested that SmHepcidin plays a defensive role against pathogenic infection. Copyright © 2012 Elsevier Ltd. All rights reserved.

Zheng X.H.,Chinese Academy of Fishery Sciences
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Based on a full-sib family, the genetic linkage map was constructed with 246 microsatellite and 306 SNP markers, which was used to detect the QTLs for standard length (SL), body depth (H), body thickness (BT), and the ratio of standard length and body depth (SLH) in mirror carp by GridQTL software. The results indicated that a total of 14 related QTLs distributed on the 7 linkage groups were obtained. Seven QTLs were related to standard length, of which the linkage groups of LG6, LG17, LG21, LG23, and LG35 were at 5% significant level, and linkage group LG1 and LG28 were at 1% significant level, which explained 6.6%-12.6% of the phenotypic variance. Three QTLs were identified for body depth on the linkage groups of LG17, LG23 and LG28 (P amp; 0.01), accounting for 11.6%, 12.7%, and 15.6% of the phenotypic variance, respectively. Two QTLs were associated with body thickness on the linkage of LG23 and LG28 (P amp; 0.05), which explained 8.6% and 7.2% of the phenotypic variation, respectively. Two QTLs were responsible for the ratio of standard length and body depth on the linkage of LG21 and LG35 (P amp; 0.05), both of which explained 8.2% of the phenotypic variance. The results provide a useful reference for further candidate gene research and molecular marker assisted selection in mirror carp.

Chinese Academy of Fishery Sciences | Date: 2014-10-15

A method for constructing a cage body of a tapered cage assembled in a rigid-flexible manner includes steps as follows. A cage body of a tapered aquaculture cage is assembled by combining a rigid-structure copper alloy stretched mesh and a flexible synthetic fiber mesh. Pipe rings mounted on the standard component of the synthetic fiber mesh are mated in a staggered manner with plugging rings on the standard component of the copper alloy stretched mesh, and high-performance synthetic fiber rope is rove through the pipe rings and the plugging rings; in this way, the connection is completed. The cage effectively improves the cage resilience under the ocean current condition, avoids mesh sheet deformation or corner breakage, enhances the water exchange capability in and out of the cage body of the gravity cage, and effectively improves the growth environment for the fishes farmed in the cage body.

The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line of Carassius auratus gibelio and establishing method and use thereof. The cell line is deposited in China Center for Type Culture Collection under an accession number of CCTCC No: C2013179. The brain tissue cell line of Carassius auratus gibelio is in good growth state and sensitive to CyHV-2 that is presently hardly cultured with ordinary fish cell lines. After six passages of CyHV-2 in GiCB cells, viral nucleic acid can still be detected and a cytopathic effect is stable. When ultrathin microscopic sections are prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and their replication process can be observed in GiCB cells. The construction method of the brain tissue cell line of Carassius auratus gibelio of the present invention has high repeatability, scientific and reasonable conditions.

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