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PubMed | Guangdong Pharmaceutical University and China Tobacco Yunnan Industrial Co.
Type: Journal Article | Journal: Natural product research | Year: 2016

Two new compounds, 19-hydroxy-melodinine K (1) and melodiside (2), and 25 known compounds were isolated from leaves and twigs of Melodinus suaveolens. Their structures were elucidated based on 1- and 2-D NMR, FTIR, UV and MS spectroscopic data. 19-hydroxy-melodinine K showed cytotoxic activity against MDA-MB-231 breast, BCG-823 gastric, SW480 colon and Hela cancer cells.


A method of ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was developed for the simultaneous quantification of 11 sesquiterpene lactones in 11 Jerusalem artichoke leaf samples harvested in a number of areas at different periods. The optimal chromatographic conditions were achieved on a ZORBAX Eclipse Plus C


PubMed | University of Agricultural science, Yunnan Agricultural University and China Tobacco Yunnan Industrial Co.
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Endophyte microorganisms live inside plants without causing them any apparent damage. Recently, endophytic microorganisms have attracted attention because they can produce bioactive compounds of biotechnological interest. The endophytic microorganisms in Paris polyphylla var. yunnanensis (Liliaceae) - a species used since antiquity in traditional Chinese medicine - are under scrutiny because they may be responsible for producing the bioactive metabolites associated with the plant. The levels of bioactive metabolites in the rhizomes of P. polyphylla increase with rhizome age. To elucidate the roles played by endophytes in the accumulation of bioactive metabolites, we investigated the community structure and diversity of the endophytic microorganisms in P. polyphylla rhizomes of different ages (4, 6, and 8 years) using 16S rRNA and internal transcribed spacer (ITS) sequence analysis. 16S rDNA amplicon pyrosequencing revealed that the number of operational taxonomic units was lower in the 8-year-old samples than in the other samples. A total of 28 phyla were observed in the P. polyphylla samples and the predominant bacteria were of the Cyanobacteria and Proteobacteria phyla. Moreover, the percentage of Cyanobacteria increased with rhizome age. Similarly, ITS1 amplicon pyrosequencing identified developmental changes in the most abundant fungal classes; some classes were more prevalent in the 8-year-old rhizomes than in younger rhizomes, indicating the importance in secondary metabolism in older rhizomes. Our study showed that endophyte microorganism diversity and prevalence depend on P. polyphylla rhizome age. There was also an indication that some endophyte microorganisms contribute to the higher saponin content in older P. polyphylla specimens.


PubMed | China Tobacco Yunnan Industrial Co., CAS Kunming Institute of Zoology and Kunming University of Science and Technology
Type: | Journal: Scientific reports | Year: 2016

Elongator protein 3 (Elp3) is the enzymatic unit of the elongator protein complex, a histone acetyltransferase complex involved in transcriptional elongation. It has long been shown to play an important role in cell migration; however, the underlying mechanism is unknown. Here, we showed that Elp3 is expressed in pre-migratory and migrating neural crest cells in Xenopus embryos, and knockdown of Elp3 inhibited neural crest cell migration. Interestingly, Elp3 binds Snail1 through its zinc-finger domain and inhibits its ubiquitination by -Trcp without interfering with the Snail1/Trcp interaction. We showed evidence that Elp3-mediated stabilization of Snail1 was likely involved in the activation of N-cadherin in neural crest cells to regulate their migratory ability. Our findings provide a new mechanism for the function of Elp3 in cell migration through stabilizing Snail1, a master regulator of cell motility.


Ling J.,China Tobacco Yunnan Industrial Co. | Ling J.,Sun Yat Sen University | Rong M.-Z.,Sun Yat Sen University | Zhang M.-Q.,Sun Yat Sen University
Chinese Journal of Polymer Science (English Edition) | Year: 2014

Polyurethanes consisting of tri-functional homopolymer of hexamethylene diisocyanate (tri-HDI) and polyethylene glycol (PEG) are synthesized, in which photo-reversible coumarin moieties act as pendant groups. Accordingly, the polyurethanes can be repeatedly self-healed under UV lights at room temperature by taking advantages of the photodimerization and photocleavage habits of coumarin. Molecular weight of the soft segment, PEG, is found to be closely related to the healing performance of the polyurethanes. Lower molecular weight PEG that corresponds to higher initial coumarin concentration in the polymer is critical for obtaining higher healing efficiency in the case of the first healing action. Nevertheless, it does not guarantee high reversibility of the photo-remendability during the repeated healing events. In contrast, the polyurethane with moderate molecular weight PEG has achieved balanced performance. Reaction kinetics is less important for the healing effect. © 2014 Chinese Chemical Society, Institute of Chemistry, Chinese Academy of Sciences and Springer-Verlag Berlin Heidelberg.


PubMed | Sun Yat Sen University, Southwest forestry University, Yunnan University, King Saud University and China Tobacco Yunnan Industrial Co.
Type: Journal Article | Journal: Antonie van Leeuwenhoek | Year: 2016

A Gram-staining negative, aerobic, rod-shaped bacterium, designated YIM C01544(T), was isolated from a soil sample collected from Sigangli Cave, Yunnan province, South-West China. The strain was able to grow over a range of temperatures (4-30C), pH (6.0-10.0) and NaCl concentration (0-2%, w/v). Comparative 16S rRNA gene sequence analysis revealed that strain YIM C01544(T) should be a member of the genus Lysobacter. The strain is closely related to Lysobacter niastensis GH41-7(T) (97.6%), Lysobacter soli DCY21(T) (97.5%), Lysobacter enzymogenes DSM 2043(T) (97.3%), Lysobacter antibioticus DSM 2044(T) (97.1%) and Lysobacter panacisoli CJ29(T) (97.1%). The genomic DNA relatedness values (<47%) as indicated by DNA-DNA hybridization studies were below the threshold limit for characterization of new bacterial species. The chemotaxonomic features of the new isolate include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and two unidentified polar lipids as its characteristic polar lipids and Q-8 as the only quinone. The major fatty acids detected were iso-C15:0 and iso-C17:1 9c. The DNA G+C content of the strain was determined to be 64.9mol%. Based on the data from phenotypic, chemotaxonomic and molecular studies, strain YIM C01544(T) merits recognition as novel species in the genus Lysobacter for which the name Lysobacter cavernae sp. nov. is proposed. The type strain of Lysobacter cavernae is YIM C01544(T) (=KCTC 42875(T)=DSM 101561(T)=CPCC 100816(T)).


PubMed | King Saud University, Jiujiang University, Sun Yat Sen University and China Tobacco Yunnan Industrial Co.
Type: Journal Article | Journal: Applied microbiology and biotechnology | Year: 2016

An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074(T) by using bioassay methods. Based on the data of (1)H-NMR, (13)C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity mainly against Microcystis. L-valine exhibited greater antialgal activities than both L-lysine and copper sulfate (CuSO4) did on Microcystis aeruginosa lawn. However, M. aeruginosa recovered growth earlier with higher growth rate in L-valine treatment than in L-lysine treatment. L-valine dissipated completely within 2days, much quicker than L-lysine (6days), which resulted in the lysing of more than 80% M. aeruginosa cells and the release of amount of intracellular microcystin-LR (MC-LR) within 2days. As a resultant, the extracellular MC-LR content was more than twice of the control from day 1 to 5. Exposure to L-valine significantly promoted the synthesis of MC-LR. L-lysine also promoted the release and synthesis of MC-LR with much lesser efficiency than L-valine. L-valine could damage Microcystis severely, causing perforation and collapse of M. aeruginosa cells and decrease of the chlorophyll. The superoxide dismutase (SOD) activity in L-valine-treated cells of M. aeruginosa initially increased with 32.943.37% higher than the control after 36h and then decreased quickly. However, the increase rate of superoxide anion radical (O2 (-)) was much higher than that of SOD, which resulted in serious lipid peroxidation and accumulation of malondialdehyde (MDA). To our knowledge, this is the first report showing L-valine active against cyanobacteria.


Zhou M.,China Tobacco Yunnan Industrial Co. | Zhou M.,Yunnan Nationalities University | Miao M.-M.,China Tobacco Yunnan Industrial Co. | Du G.,Yunnan Nationalities University | And 9 more authors.
Organic Letters | Year: 2014

Aspergillines A-E (1-5) are highly oxygenated cyclopiazonic acid (CPA)-derived alkaloids bearing a rigid and sterically congested hexacyclic indole-tetrahydrofuran-tetramate scaffold, isolated from the endophytic fungus Aspergillus vesicolor. Apergillines A-C represent a new subclass of CPA-derived alkaloids, and aspergillines B and E possess a butanoic acid methyl ester moiety. The structures, including absolute configuration, were elucidated by interpretation of the NMR, X-ray crystallographic, and circular dichroism data. All compounds displayed anti-TMV and cytotoxic activities. © 2014 American Chemical Society.


Patent
China Tobacco Yunnan Industrial Co. | Date: 2016-03-16

This invention relates to an electric-heating smoking article comprising an igniting activator, the electric-heating smoking article comprising a mouthpiece 20 on its one end, and the igniting activator 10 on its other end, wherein the igniting activator 10 comprises at least one temperature measurement element 1 and a heat conductor 2 contacting with the temperature measurement element 1, wherein the temperature measurement element 1 is connected to a positive wire terminal of a heating element 5 and a negative wire terminal of a power supply 6. When the heat conductor 2 is heated to a temperature above a specified value, the smoking article activates the heating element to heat, enabling consumers to get used to novel electric-heating cigarettes without changing their traditional smoke behaviors while keeping the igniting manner unchanged. This invention also discloses an igniting activation method of an electric-heating smoking article.


PubMed | Yunnan University, Kunming Medical University and China Tobacco Yunnan Industrial Co.
Type: | Journal: Journal of Asian natural products research | Year: 2016

Three new benzolactones (1-3), together with four known ones (4-7), were isolated from the whole herb of Lavandula angustifolia. Their structures were established on the basis of detailed spectroscopic analysis (1D- and 2D-NMR, HRESIMS, UV, and IR) and comparison with data reported in the literature. New compounds were evaluated for their anti-tobacco mosaic virus (TMV) activities and cytotoxic activities. The results revealed that compounds 1-3 showed obvious anti-TMV activities with inhibition rates of 26.9, 30.2, and 28.4%, which were at the same grade as positive control. Compounds 1-3 also showed weak inhibitory activities against some tested human tumor cell lines with IC

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